FastQCFastQC Report
Fri 10 Feb 2017
SRR2031805_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031805_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700373
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15660.22359514144605802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13640.19475336713436983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7610.10865638738215208No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11050.049.864251
GTATCAA19600.039.0178531
TCAACGC22150.033.668174
ATCAACG22700.033.0616723
CAACGCA22600.032.9977875
TATCAAC23350.032.1413272
AACGCAG23850.031.2683456
GTATACC953.4889166E-530.01
ACGCAGA26450.027.6559567
CGCAGAG26800.027.1175378
GTACATG18200.025.3159351
TACATGG18650.023.6863252
GAGTACT18100.023.61878612-13
GCAGAGT31100.023.3681669
ACATGGG18650.023.1769433
AGAGTAC25400.022.44094710-11
CATGGGG11200.022.053574
TATAGTG2901.05501385E-1021.2931025
CAGAGTA28550.021.21278410-11
GTACTTT20500.020.73780614-15
Produced by FastQC (version 0.11.5)