##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031805_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 700373 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.606675300161484 34.0 31.0 34.0 31.0 34.0 2 32.73595212836589 34.0 31.0 34.0 31.0 34.0 3 32.816840455014685 34.0 33.0 34.0 31.0 34.0 4 35.99367337118935 37.0 37.0 37.0 35.0 37.0 5 36.01396969900325 37.0 37.0 37.0 35.0 37.0 6 36.08690940398902 37.0 37.0 37.0 35.0 37.0 7 36.10785966906206 37.0 37.0 37.0 35.0 37.0 8 36.10097191068188 37.0 37.0 37.0 35.0 37.0 9 37.94261200817279 39.0 39.0 39.0 37.0 39.0 10-11 37.97820732666736 39.0 39.0 39.0 37.0 39.0 12-13 37.956798020483376 39.0 39.0 39.0 37.0 39.0 14-15 39.55051879498495 41.0 40.0 41.0 37.0 41.0 16-17 39.48238724222664 41.0 40.0 41.0 37.0 41.0 18-19 39.47818019826578 41.0 40.0 41.0 37.0 41.0 20-21 39.41563923794892 41.0 40.0 41.0 37.0 41.0 22-23 39.33042464515337 41.0 40.0 41.0 37.0 41.0 24-25 39.294690829029676 41.0 40.0 41.0 36.5 41.0 26-27 39.182268591165 41.0 39.5 41.0 36.0 41.0 28-29 39.05713669715995 41.0 39.0 41.0 36.0 41.0 30-31 38.91559283410411 41.0 39.0 41.0 35.5 41.0 32-33 38.80559644646495 41.0 39.0 41.0 35.0 41.0 34-35 38.620178819000735 40.5 38.5 41.0 35.0 41.0 36-37 38.50636732141302 40.0 38.0 41.0 35.0 41.0 38-39 38.322261137993614 40.0 38.0 41.0 34.0 41.0 40-41 38.225634054996405 40.0 38.0 41.0 34.0 41.0 42-43 38.061462963306695 40.0 38.0 41.0 33.5 41.0 44-45 38.23933532560507 40.0 38.0 41.0 34.0 41.0 46-47 38.12572229369208 40.0 38.0 41.0 33.5 41.0 48-49 37.96506433000701 40.0 38.0 41.0 33.0 41.0 50-51 36.895071626119226 39.0 36.5 40.0 31.5 40.5 52-53 36.90578306131161 39.5 37.0 40.0 32.0 40.5 54-55 37.667592839815356 40.0 37.0 41.0 33.0 41.0 56-57 37.643466410041505 40.0 37.0 41.0 33.0 41.0 58-59 37.49673673885201 40.0 37.0 41.0 33.0 41.0 60-61 37.266892070368215 40.0 36.0 41.0 33.0 41.0 62-63 36.974903372917 39.0 35.5 41.0 32.0 41.0 64-65 36.63880389449622 39.0 35.0 41.0 31.5 41.0 66-67 36.34450571338415 38.5 35.0 41.0 31.0 41.0 68-69 36.002602184835794 37.0 35.0 40.5 31.0 41.0 70-71 35.629315379090855 37.0 35.0 39.5 31.0 41.0 72-73 35.226530006153865 36.5 35.0 39.0 30.0 41.0 74-75 34.86433229150752 36.0 35.0 39.0 30.0 41.0 76-77 34.50430413508231 35.5 35.0 37.5 30.0 39.5 78-79 34.14738789188047 35.0 35.0 37.0 29.0 39.0 80-81 33.81379707670055 35.0 34.5 37.0 29.0 39.0 82-83 33.553248054964996 35.0 34.0 36.0 29.0 38.0 84-85 33.27405753791194 35.0 34.0 36.0 29.0 37.0 86-87 33.037904088250116 35.0 34.0 35.5 28.5 37.0 88-89 32.86031300464181 35.0 34.0 35.0 27.0 36.0 90-91 32.70207517993983 35.0 34.0 35.0 27.0 36.0 92-93 32.590443235247506 35.0 34.0 35.0 27.0 36.0 94-95 32.43896966330798 35.0 34.0 35.0 26.0 36.0 96-97 32.351095773252254 35.0 34.0 35.0 25.5 35.0 98-99 32.257297896977754 35.0 34.0 35.0 25.0 35.0 100-101 30.691305204512453 34.0 30.5 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17780568025409593 1101 2 0.1922020717465287 1101 3 0.2967006025793353 1101 4 0.1518487716265824 1101 5 0.18530131124471438 1101 6 0.1842272800475797 1101 7 0.21363223510507368 1101 8 0.254161966677799 1101 9 0.18646931287243262 1101 10-11 0.26655392212226303 1101 12-13 0.3095093082960574 1101 14-15 0.282818606748549 1101 16-17 0.403991968907917 1101 18-19 0.42484764482080095 1101 20-21 0.4752233235211918 1101 22-23 0.3874141045994577 1101 24-25 0.42014673299105 1101 26-27 0.31572799317289935 1101 28-29 0.47980489129311366 1101 30-31 0.355265018025932 1101 32-33 0.279738778080187 1101 34-35 0.3238125903265754 1101 36-37 0.5539776186694638 1101 38-39 0.6149783904397665 1101 40-41 0.33057496167679545 1101 42-43 0.42544528550082106 1101 44-45 0.6023908486688043 1101 46-47 0.6855347742584215 1101 48-49 0.4074826485926337 1101 50-51 0.4297356278732636 1101 52-53 0.4522458184678726 1101 54-55 0.44184515040708305 1101 56-57 0.578627421631893 1101 58-59 0.49610905415991624 1101 60-61 0.4218979388519273 1101 62-63 0.5621672183791091 1101 64-65 0.6031854284222078 1101 66-67 0.43907532499552815 1101 68-69 0.4473581336855901 1101 70-71 0.4861273589016264 1101 72-73 0.6060632331684204 1101 74-75 0.4471496485834905 1101 76-77 0.3992477203986553 1101 78-79 0.41956919560947625 1101 80-81 0.5867499603356876 1101 82-83 0.6639502740138283 1101 84-85 0.4861789732550861 1101 86-87 0.6928812953211221 1101 88-89 0.7486950041222897 1101 90-91 0.716317434901903 1101 92-93 0.6983244666474988 1101 94-95 0.6934091789544823 1101 96-97 0.5222068505005595 1101 98-99 0.3452060346681307 1101 100-101 0.6726618279603969 1102 1 0.2753914203263008 1102 2 0.2364885739527054 1102 3 0.2278453077538245 1102 4 0.22772372148618558 1102 5 0.21549836298959946 1102 6 0.22687795192884863 1102 7 0.20060200044803622 1102 8 0.24516803366536521 1102 9 0.2692197641359684 1102 10-11 0.4360033806060173 1102 12-13 0.39486706000083416 1102 14-15 0.3984047479438999 1102 16-17 0.3681290527426526 1102 18-19 0.5467013351537275 1102 20-21 0.5281151706932832 1102 22-23 0.49918017459545183 1102 24-25 0.5164601605883234 1102 26-27 0.5079537252226416 1102 28-29 0.5759647639365113 1102 30-31 0.5711413467463515 1102 32-33 0.43018502757877997 1102 34-35 0.39099131575778046 1102 36-37 0.32341015477054214 1102 38-39 0.40889197347225803 1102 40-41 0.4194801461934432 1102 42-43 0.3531146346785121 1102 44-45 0.4733921523190219 1102 46-47 0.67899458673336 1102 48-49 0.6397454845332717 1102 50-51 0.7452785229795609 1102 52-53 0.6807199209548429 1102 54-55 0.7454201604351667 1102 56-57 0.8214980844878852 1102 58-59 0.6178373935100936 1102 60-61 0.6971906333966587 1102 62-63 0.5042268603766971 1102 64-65 0.5515846963724655 1102 66-67 0.8090456168126181 1102 68-69 0.5381404491689423 1102 70-71 0.5089349702132893 1102 72-73 0.6246277137139415 1102 74-75 0.49751567345312253 1102 76-77 0.4900927113733857 1102 78-79 0.6890017317105546 1102 80-81 0.6590900124816983 1102 82-83 0.5278316418437541 1102 84-85 0.5885813248395237 1102 86-87 0.5949633751446228 1102 88-89 0.5721256098182366 1102 90-91 0.5872685981590209 1102 92-93 0.5838226114529519 1102 94-95 0.48330839676434323 1102 96-97 0.3795386294817078 1102 98-99 0.5294719793131364 1102 100-101 0.40002030670086697 1103 1 0.3038885655139012 1103 2 0.2085918456583471 1103 3 0.32747486185931507 1103 4 0.22878294751482287 1103 5 0.07868827598579031 1103 6 0.14303199851213577 1103 7 0.20363523862095434 1103 8 0.2297922633191618 1103 9 0.18655765157479465 1103 10-11 0.20568119463538181 1103 12-13 0.22796532445615725 1103 14-15 0.3207527178550862 1103 16-17 0.16628135471836458 1103 18-19 0.3416929519744656 1103 20-21 0.350054743830853 1103 22-23 0.295671417571711 1103 24-25 0.34107328819390403 1103 26-27 0.35284791590597564 1103 28-29 0.2799974696642664 1103 30-31 0.2810281171831832 1103 32-33 0.3135342209809906 1103 34-35 0.48672240828995683 1103 36-37 0.48979218984395345 1103 38-39 0.707360060747952 1103 40-41 0.5720738338860301 1103 42-43 0.6422564350229081 1103 44-45 0.6261642657864854 1103 46-47 0.6415067650005284 1103 48-49 0.581420081234377 1103 50-51 0.36866561061777503 1103 52-53 0.5867900791023857 1103 54-55 0.6749901259961533 1103 56-57 0.6367778447742722 1103 58-59 0.6154413902474474 1103 60-61 0.7391192744153301 1103 62-63 0.7549193223125386 1103 64-65 0.6969790456564979 1103 66-67 0.5429660559099503 1103 68-69 0.5843258193882619 1103 70-71 0.6348440920186249 1103 72-73 0.647505863070478 1103 74-75 0.6310914392678768 1103 76-77 0.6136015816833336 1103 78-79 0.569086866294569 1103 80-81 0.5475540780970718 1103 82-83 0.5886606703012447 1103 84-85 0.6042303981583572 1103 86-87 0.612973049786639 1103 88-89 0.7260022637968433 1103 90-91 0.6779372133484358 1103 92-93 0.6978253529791445 1103 94-95 0.7340390927948022 1103 96-97 0.6171989294635765 1103 98-99 0.558359961912366 1103 100-101 0.7696179707392972 1104 1 -0.026019869996282807 1104 2 -0.0537219995239937 1104 3 -0.12423802557950836 1104 4 -0.02216427134535337 1104 5 -0.012794647763094247 1104 6 -0.017868911870429827 1104 7 -0.07268428987454456 1104 8 -0.0289695111375039 1104 9 0.13645766736177478 1104 10-11 0.065438864476981 1104 12-13 0.1369257517571114 1104 14-15 0.059000626080106144 1104 16-17 0.11855467998280034 1104 18-19 0.06204631723258558 1104 20-21 0.23957350402660893 1104 22-23 0.16343308678182922 1104 24-25 0.19503318926215485 1104 26-27 0.16196358185346327 1104 28-29 0.29539240730569105 1104 30-31 0.4279009793628461 1104 32-33 0.31436961539240116 1104 34-35 0.3545945863671065 1104 36-37 0.4662024934802247 1104 38-39 0.5438214089561271 1104 40-41 0.45416876626512703 1104 42-43 0.47788501998316946 1104 44-45 0.6251170627132865 1104 46-47 0.6390808322530646 1104 48-49 0.5136926171497507 1104 50-51 0.49144227745984637 1104 52-53 0.6180575105605755 1104 54-55 0.716970339646636 1104 56-57 0.6555314626240829 1104 58-59 0.6490873935100936 1104 60-61 0.6351330617120681 1104 62-63 0.7434237689788503 1104 64-65 0.7964659687739015 1104 66-67 0.6734441383179899 1104 68-69 0.5261836839359617 1104 70-71 0.5650169594606069 1104 72-73 0.4739332692695015 1104 74-75 0.5133310856394999 1104 76-77 0.535506017825 1104 78-79 0.5922779413879766 1104 80-81 0.5932073959942414 1104 82-83 0.578929312094651 1104 84-85 0.549810043477521 1104 86-87 0.47158747908727605 1104 88-89 0.4009338535458298 1104 90-91 0.46309745120561985 1104 92-93 0.4550031670085062 1104 94-95 0.5392895795600481 1104 96-97 0.6310283248222035 1104 98-99 0.7031278932916294 1104 100-101 0.4426222332241636 1105 1 0.1183674265182546 1105 2 0.09825542981786128 1105 3 0.15873411064340104 1105 4 0.11348438540087358 1105 5 0.20192122160673165 1105 6 0.22152986971350686 1105 7 0.24244604069571096 1105 8 0.33278520772797293 1105 9 0.34908625020408124 1105 10-11 0.19819378308279312 1105 12-13 0.1717579698735605 1105 14-15 0.3963945140257721 1105 16-17 0.43850873970516346 1105 18-19 0.4349989787725832 1105 20-21 0.43686452569843937 1105 22-23 0.43939184040212353 1105 24-25 0.4062036676402627 1105 26-27 0.3448882797462005 1105 28-29 0.4579011459454563 1105 30-31 0.3726952468151552 1105 32-33 0.4565223168804664 1105 34-35 0.4699521215713389 1105 36-37 0.5082253637488705 1105 38-39 0.5357543960728748 1105 40-41 0.4220655005106053 1105 42-43 0.6126148711766248 1105 44-45 0.5518772744380485 1105 46-47 0.7615566137371346 1105 48-49 0.7612732623110432 1105 50-51 0.673068663158439 1105 52-53 0.7508174226748281 1105 54-55 0.8512208140299364 1105 56-57 0.8127395920558271 1105 58-59 0.8855425018692245 1105 60-61 0.6750861227327434 1105 62-63 0.6377811044427446 1105 64-65 0.8116412409201175 1105 66-67 0.6570928949343937 1105 68-69 0.6468892666784001 1105 70-71 0.7641755532886236 1105 72-73 0.7429735169475222 1105 74-75 0.7440112014888953 1105 76-77 0.7284102423331333 1105 78-79 0.9148386125155028 1105 80-81 0.9969275597813194 1105 82-83 0.8388589036256562 1105 84-85 0.9363974798102817 1105 86-87 0.891666376520611 1105 88-89 0.912446387252011 1105 90-91 1.0281978207252465 1105 92-93 0.9259009998258492 1105 94-95 0.8604046299944841 1105 96-97 0.8522607227049619 1105 98-99 0.7772789968569924 1105 100-101 0.7281909938353692 1106 1 0.36454723962185653 1106 2 0.21332092889570475 1106 3 0.2890850984633673 1106 4 0.30677659710299565 1106 5 0.34344948601154357 1106 6 0.38032305572330216 1106 7 0.4191845042765223 1106 8 0.43055970883977324 1106 9 0.3319351163564903 1106 10-11 0.3124390620761588 1106 12-13 0.4488619766249684 1106 14-15 0.4158190324427977 1106 16-17 0.5250384027511643 1106 18-19 0.4234613742893458 1106 20-21 0.43363935395004205 1106 22-23 0.40494496743193054 1106 24-25 0.415390843760008 1106 26-27 0.530504558981356 1106 28-29 0.5624946154764103 1106 30-31 0.5639961727630265 1106 32-33 0.548432007330355 1106 34-35 0.4802096137839911 1106 36-37 0.48333310653164574 1106 38-39 0.5676185240082461 1106 40-41 0.4051419421777922 1106 42-43 0.29147737289529374 1106 44-45 0.3924144000644674 1106 46-47 0.4348423300547779 1106 48-49 0.29151828528194557 1106 50-51 0.30781707368570466 1106 52-53 0.5030955484008146 1106 54-55 0.5346727552964907 1106 56-57 0.5292717679148069 1106 58-59 0.5317443613508317 1106 60-61 0.46754673557464344 1106 62-63 0.3547684313312729 1106 64-65 0.250966289872558 1106 66-67 0.3486664373843382 1106 68-69 0.3467574625260923 1106 70-71 0.26770060318930433 1106 72-73 0.23641412684989405 1106 74-75 0.2619256615422785 1106 76-77 0.21811482086802414 1106 78-79 0.21416204947471584 1106 80-81 0.33560970790453126 1106 82-83 0.2840582455531262 1106 84-85 0.2640149538168899 1106 86-87 0.43590847893907636 1106 88-89 0.28633991984035134 1106 90-91 0.22245130191944185 1106 92-93 0.274834713716011 1106 94-95 0.23692950447236427 1106 96-97 0.25950236523123493 1106 98-99 0.3388772881441682 1106 100-101 0.52612764250145 1107 1 0.22607357371862946 1107 2 0.10002716785634647 1107 3 0.25393306586591535 1107 4 0.16869594370840701 1107 5 0.175030073904054 1107 6 0.2487406608767344 1107 7 0.18663629248343483 1107 8 0.18025591468798297 1107 9 0.17643768448994734 1107 10-11 0.228591876181234 1107 12-13 0.2937270649172632 1107 14-15 0.21179584922217032 1107 16-17 0.26491494261482984 1107 18-19 0.14905507163356901 1107 20-21 0.2459894332301502 1107 22-23 0.18996034912839121 1107 24-25 0.2353177072943211 1107 26-27 0.2485203427250937 1107 28-29 0.21687399451665357 1107 30-31 0.28344622629404626 1107 32-33 0.2005267188275539 1107 34-35 0.40628599373221874 1107 36-37 0.409900752115675 1107 38-39 0.4401650191949713 1107 40-41 0.3242705837549025 1107 42-43 0.27559650537664737 1107 44-45 0.3944528701164671 1107 46-47 0.3810210739506559 1107 48-49 0.4279399289777146 1107 50-51 0.30164067883010404 1107 52-53 0.383161231180992 1107 54-55 0.49437542100546494 1107 56-57 0.33163052057442144 1107 58-59 0.31317293923280687 1107 60-61 0.43493799082045115 1107 62-63 0.3071167940050188 1107 64-65 0.41891815261632104 1107 66-67 0.2632076743347369 1107 68-69 0.21546192163697953 1107 70-71 0.28801867227819145 1107 72-73 0.43396276779457565 1107 74-75 0.2585161650952017 1107 76-77 0.3738731317273718 1107 78-79 0.4524834794981629 1107 80-81 0.4700843585977239 1107 82-83 0.5155098620994067 1107 84-85 0.3897044197264421 1107 86-87 0.5476889159509142 1107 88-89 0.3225557966422272 1107 90-91 0.531805225494324 1107 92-93 0.5429087717282712 1107 94-95 0.5090274233130145 1107 96-97 0.5533187425593837 1107 98-99 0.45535693506534614 1107 100-101 0.3366368873301724 1108 1 0.18022096578084756 1108 2 0.16704412950826253 1108 3 0.16723924714776217 1108 4 0.16964291340537585 1108 5 0.23017639329263062 1108 6 0.14891078021167914 1108 7 0.21196563681166936 1108 8 0.15930944780677692 1108 9 0.3274584004996015 1108 10-11 0.21938406242420427 1108 12-13 0.20981339838468216 1108 14-15 0.13435803077218367 1108 16-17 0.1773935729446734 1108 18-19 0.1365056280830288 1108 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-0.39782509669216637 2316 98-99 -0.5587428045083485 2316 100-101 -0.1986147758188892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 13.0 7 297.0 8 677.0 9 504.0 10 538.0 11 714.0 12 844.0 13 842.0 14 935.0 15 884.0 16 1082.0 17 1341.0 18 1645.0 19 2242.0 20 2898.0 21 3699.0 22 4148.0 23 4201.0 24 3998.0 25 3686.0 26 3746.0 27 3946.0 28 4598.0 29 5430.0 30 6689.0 31 8086.0 32 10355.0 33 14264.0 34 21277.0 35 35850.0 36 71320.0 37 162525.0 38 242202.0 39 74781.0 40 116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86633979322447 18.725165019211193 13.193398374865964 24.215096812698373 2 15.817428712985796 21.016943828502814 38.915834848002426 24.249792610508973 3 17.900889954352895 25.400322399635623 31.104425784546237 25.59436186146525 4 12.92297104542865 17.720557474374367 38.197074987185395 31.15939649301158 5 13.657436822950055 37.59211163194469 35.08030720773074 13.670144337374513 6 30.828001650549062 37.51872216661693 17.888753564172234 13.76452261866177 7 26.370234146661854 33.12035158408448 22.686197212057007 17.823217057196665 8 25.528968135550627 34.23618557540053 21.165864475072567 19.068981813976265 9 26.39978982627828 15.00500447618626 20.38813603608363 38.207069661451825 10-11 24.023270457313462 25.758917034208917 29.85444898646864 20.36336352200899 12-13 25.08370539698132 24.144206015937222 28.750822775863718 22.021265811217734 14-15 22.636652183907717 25.59850251223277 26.334681662485558 25.430163641373955 16-17 22.164261043758113 28.24580616328728 27.008179926981768 22.581752865972845 18-19 22.13713264217781 27.603148607956047 28.55785417199121 21.701864577874932 20-21 23.030370959474453 27.071788889634522 28.25894202089458 21.638898129996445 22-23 22.734742772779647 27.052941789589262 28.016000045689935 22.19631539194115 24-25 22.584712124413883 27.18790871137053 27.965852432707187 22.2615267315084 26-27 22.57497076557777 27.227991370312676 28.018998447969867 22.178039416139686 28-29 22.527496062812244 27.069789954781236 28.20539912303872 22.197314859367793 30-31 22.791712416098278 27.01974519291863 28.094244067089964 22.094298323893124 32-33 22.51343212830877 27.231917849488774 28.00007995739413 22.254570064808323 34-35 22.845755047667456 27.190154389161204 27.914625492416185 22.049465070755154 36-37 22.76458401451798 27.267398943134584 27.79590304023713 22.172114002110305 38-39 22.705972388998433 27.166452733043677 27.889281854097746 22.23829302386014 40-41 22.850181260556877 26.981551259114784 27.938541320125132 22.229726160203207 42-43 22.718108779179094 27.23784326351815 27.724226947640755 22.319821009661993 44-45 22.679843454844775 27.41996050675854 27.50191683574324 22.39827920265344 46-47 22.76122865958568 27.247623766193158 27.439735683699972 22.551411890521194 48-49 22.6358668880725 27.39747248965908 27.426956778745037 22.53970384352338 50-51 22.652929224855896 27.416390980234816 27.40625352490744 22.52442627000184 52-53 22.794996380500105 27.405825181724598 27.32936592358643 22.469812514188867 54-55 22.675345851424883 27.379196513857618 27.35278201758206 22.59267561713544 56-57 22.533707038963524 27.49570585959196 27.267613114726007 22.702973986718504 58-59 22.730744903073077 27.41674793288719 27.29195728561781 22.560549878421927 60-61 22.642934550589473 27.26968344010977 27.369772963834986 22.71760904546577 62-63 22.50764949534034 27.35342453235633 27.450015920088294 22.688910052215032 64-65 22.516287749527752 27.26375802608039 27.388120330166927 22.831833894224935 66-67 22.469883904719342 27.36891627746929 27.336647757694827 22.824552060116538 68-69 22.510290944967895 27.35499512402677 27.3440009823337 22.790712948671636 70-71 22.506435856322273 27.149533177321228 27.296883232220548 23.047147734135955 72-73 22.38878426210034 27.195080335763944 27.32872340881216 23.08741199332356 74-75 22.41005864018173 27.138253473506257 27.382623259320393 23.06906462699162 76-77 22.39121154013647 27.09663279423964 27.361634443360895 23.15052122226299 78-79 22.337811423341563 27.102272646147124 27.365560922536993 23.19435500797432 80-81 22.29983166112914 27.139324331463378 27.370201307017833 23.19064270038965 82-83 22.287909442539906 27.151817674296407 27.400328110878057 23.159944772285627 84-85 22.314395329345935 26.87567910242114 27.423315861690845 23.386609706542085 86-87 22.13948852968347 26.925509692692323 27.499632338768055 23.435369438856153 88-89 22.22437187041762 26.854761676992116 27.384836365765096 23.536030086825164 90-91 22.18881938624133 26.685923072419982 27.56702499953596 23.558232541802727 92-93 22.19931379422108 26.580764821031078 27.648267423215916 23.571653961531926 94-95 22.077450158701147 26.628953429101347 27.67054126872395 23.623055143473547 96-97 22.05974530714348 26.55770567968782 27.646054316771206 23.736494696397493 98-99 21.985142202797654 26.543855916775776 27.63734467205332 23.833657208373253 100-101 22.239078319695363 26.198682701931688 27.761064461365585 23.801174517007368 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 197.0 1 128.5 2 73.5 3 90.0 4 86.0 5 79.0 6 112.0 7 206.0 8 333.5 9 429.0 10 395.0 11 340.0 12 372.0 13 427.5 14 468.5 15 509.0 16 562.5 17 615.0 18 688.5 19 767.5 20 868.5 21 982.0 22 1152.0 23 1386.5 24 1748.5 25 2179.0 26 2743.0 27 3594.0 28 4594.0 29 5525.0 30 6689.0 31 8375.0 32 10088.0 33 11842.0 34 13663.0 35 15448.5 36 17387.5 37 18828.5 38 20081.5 39 21513.5 40 22468.0 41 23269.5 42 24113.0 43 24874.0 44 25756.0 45 26569.0 46 27197.5 47 28084.0 48 29151.0 49 29137.0 50 28066.0 51 27026.0 52 26190.5 53 25229.5 54 23616.0 55 21489.0 56 19252.5 57 17210.0 58 15390.0 59 13096.0 60 10637.5 61 8574.0 62 6745.0 63 5124.0 64 3857.5 65 3023.0 66 2356.5 67 1761.5 68 1306.5 69 987.5 70 742.0 71 578.0 72 448.0 73 355.0 74 277.5 75 230.0 76 191.5 77 124.0 78 78.0 79 65.5 80 42.5 81 34.0 82 36.0 83 34.5 84 26.5 85 17.0 86 15.0 87 12.5 88 8.5 89 5.5 90 3.5 91 4.0 92 4.5 93 3.0 94 2.5 95 3.5 96 1.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.4278106094895148E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 700373.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.87936587733166 #Duplication Level Percentage of deduplicated Percentage of total 1 72.5164949472372 45.59791217929001 2 15.668371588070649 19.704345395765646 3 5.613825658180471 10.589813925968462 4 2.5617236247676423 6.443182283134755 5 1.3566705352890904 4.265329148171905 6 0.7758385976853368 2.927054342736733 7 0.468776695466081 2.063346694428473 8 0.2991952488225653 1.5050566015578697 9 0.19480766231698698 1.1024444047083752 >10 0.5323929316057052 4.740786782766246 >50 0.009613623967386437 0.38759692178523125 >100 0.0016022206138251488 0.14190880508436016 >500 2.2888865902758948E-4 0.10952596413223553 >1k 4.5777731805517896E-4 0.421696550469711 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1566 0.22359514144605802 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1364 0.19475336713436983 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 761 0.10865638738215208 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 2.8556212189790296E-4 10-11 0.0 0.0 0.0 0.0 2.8556212189790296E-4 12-13 0.0 0.0 0.0 1.4278106094895148E-4 2.8556212189790296E-4 14-15 0.0 0.0 0.0 2.1417159142342722E-4 2.8556212189790296E-4 16-17 0.0 0.0 0.0 2.8556212189790296E-4 5.711242437958059E-4 18-19 0.0 0.0 0.0 3.5695265237237867E-4 5.711242437958059E-4 20-21 0.0 0.0 0.0 5.711242437958059E-4 5.711242437958059E-4 22-23 0.0 0.0 0.0 0.0016419822009129423 5.711242437958059E-4 24-25 0.0 0.0 0.0 0.0034981359932493112 5.711242437958059E-4 26-27 0.0 0.0 0.0 0.00721044357792205 5.711242437958059E-4 28-29 0.0 0.0 0.0 0.020417691715700063 5.711242437958059E-4 30-31 0.0 0.0 0.0 0.04076399290092565 5.711242437958059E-4 32-33 0.0 0.0 0.0 0.06996271986498623 5.711242437958059E-4 34-35 0.0 0.0 0.0 0.10844221579072866 7.139053047447575E-4 36-37 0.0 0.0 0.0 0.1733362079920271 7.139053047447575E-4 38-39 0.0 0.0 0.0 0.2619318563108515 7.139053047447575E-4 40-41 0.0 0.0 0.0 0.3642344864807753 7.139053047447575E-4 42-43 0.0 0.0 0.0 0.45068841888536537 7.139053047447575E-4 44-45 0.0 0.0 0.0 0.5465659013125863 7.852958352192332E-4 46-47 0.0 0.0 0.0 0.6753544182885405 8.566863656937089E-4 48-49 0.0 0.0 0.0 0.8112105977814679 8.566863656937089E-4 50-51 0.0 0.0 0.0 0.9484231973534103 8.566863656937089E-4 52-53 0.0 0.0 0.0 1.0789964775912264 8.566863656937089E-4 54-55 0.0 0.0 0.0 1.231843603337079 8.566863656937089E-4 56-57 0.0 0.0 0.0 1.4232416155391485 8.566863656937089E-4 58-59 0.0 0.0 0.0 1.6367706921883054 8.566863656937089E-4 60-61 0.0 0.0 0.0 1.8473727570880087 8.566863656937089E-4 62-63 0.0 0.0 0.0 2.0508357689402645 8.566863656937089E-4 64-65 0.0 0.0 0.0 2.2772151410748274 8.566863656937089E-4 66-67 0.0 0.0 0.0 2.552211464462508 8.566863656937089E-4 68-69 0.0 0.0 0.0 2.8831779637421775 8.566863656937089E-4 70-71 0.0 0.0 0.0 3.2267805869158295 8.566863656937089E-4 72-73 0.0 0.0 0.0 3.5883022332385743 9.994674266426604E-4 74-75 0.0 0.0 0.0 3.956677370486869 9.994674266426604E-4 76-77 0.0 0.0 0.0 4.359676915015285 9.994674266426604E-4 78-79 0.0 0.0 0.0 4.802726547139882 9.994674266426604E-4 80-81 0.0 0.0 0.0 5.293036710438581 9.994674266426604E-4 82-83 0.0 0.0 0.0 5.8191135295049925 9.994674266426604E-4 84-85 0.0 0.0 0.0 6.387453542612294 9.994674266426604E-4 86-87 0.0 0.0 0.0 7.027255476724545 9.994674266426604E-4 88-89 0.0 0.0 0.0 7.664344570678767 9.994674266426604E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1105 0.0 49.86425 1 GTATCAA 1960 0.0 39.017853 1 TCAACGC 2215 0.0 33.66817 4 ATCAACG 2270 0.0 33.061672 3 CAACGCA 2260 0.0 32.997787 5 TATCAAC 2335 0.0 32.141327 2 AACGCAG 2385 0.0 31.268345 6 GTATACC 95 3.4889166E-5 30.0 1 ACGCAGA 2645 0.0 27.655956 7 CGCAGAG 2680 0.0 27.117537 8 GTACATG 1820 0.0 25.315935 1 TACATGG 1865 0.0 23.686325 2 GAGTACT 1810 0.0 23.618786 12-13 GCAGAGT 3110 0.0 23.368166 9 ACATGGG 1865 0.0 23.176943 3 AGAGTAC 2540 0.0 22.440947 10-11 CATGGGG 1120 0.0 22.05357 4 TATAGTG 290 1.05501385E-10 21.293102 5 CAGAGTA 2855 0.0 21.212784 10-11 GTACTTT 2050 0.0 20.737806 14-15 >>END_MODULE