FastQCFastQC Report
Fri 10 Feb 2017
SRR2031805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700373
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT22590.3225424166836814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18960.270712891559212No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18180.2595759688051938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10100.144208871558441No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11700.049.1899531
GTATCAA20650.039.1566731
GTTTACG550.002562007434.5918851
TCAACGC24850.032.685654
CAACGCA24900.032.6200185
AACGCAG25400.032.1648946
ATCAACG25650.031.6662143
TATCAAC26350.031.0052492
ACGCAGA27800.029.0463497
CGCAGAG27950.028.5505778
GCAGAGT31950.024.232839
GAGTACT20100.023.51334612-13
GTAATAC1452.3329161E-522.9307083
ATAGCCC1503.031365E-522.166353
AGAGTAC28100.021.80573810-11
CAGAGTA31100.021.0768710-11
GTACTTT22700.020.82018914-15
TACACTG5350.019.532435
AGTACTT22300.019.38313112-13
GTACATG15750.018.421551
Produced by FastQC (version 0.11.5)