FastQCFastQC Report
Fri 10 Feb 2017
SRR2031804_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031804_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314108
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24330.77457435022349No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24260.7723458173621811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12700.4043195334088912No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11220.3572019814840755No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.22221656245622526No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.10442268264418608No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3240.10314923529486675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11550.054.2857171
GTATCAA19200.036.3671881
GTGTACC550.00257678534.5454524
CATGGGT1751.5097612E-1029.8571434
ATCAACG23600.029.1843223
CAACGCA23800.028.9390755
TCAACGC23700.028.8607584
TATCAAC24050.028.8357582
AACGCAG24500.028.1122466
ATGGGTA1457.856979E-726.2068965
CATGGGG4400.025.909094
GTACATG12350.025.7692321
TACATGG12100.025.5165312
ACGCAGA27000.025.3333347
TTGTACT1701.1174052E-725.1470584
ACATGGG11600.024.9784493
CGCAGAG26700.024.9063668
CCTATGT1151.2788575E-424.7826083
GAGTACT18150.024.20798912-13
GCAGAGT27500.024.009099
Produced by FastQC (version 0.11.5)