FastQCFastQC Report
Fri 10 Feb 2017
SRR2031804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314108
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29700.9455346568696118No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29680.9448979331949522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15740.5011015319571612No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT10540.3355533765456467No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9090.28939091013282053No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9020.28716237727151167No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.13753231372648897No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.1305283533052326No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.12861818228125357No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3670.11683879430004966No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.055.3686641
CGCAGAA652.5836653E-643.845359
GTATCAA21650.035.1032752
ATCAACG25800.029.456824
TCAACGC25800.029.2727155
CAACGCA26200.028.8258066
AACGCAG26350.028.661717
TATCAAC26450.028.5533493
TAAGGCA1658.370989E-825.9086134
ACGCAGA29000.025.8788348
CGCAGAG28950.025.2672378
GCCTAGC1000.001634393223.7912221
GTACATG12700.023.603891
GCAGAGT30350.023.475689
GAGTACT21050.023.467512-13
TACATGG13050.023.294592
CAGAGTA30100.023.2761510-11
ATGGGAG3500.023.0710035
ACATGGG12250.022.8771293
TCTAGGG1050.002192930322.618633
Produced by FastQC (version 0.11.5)