FastQCFastQC Report
Fri 10 Feb 2017
SRR2031803_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031803_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960219
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96060.490047285532892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78170.3987819728305868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46600.23772853951522763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39200.066.887761
GTATCAA74750.044.481611
TCAACGC92650.035.47764
ATCAACG93300.035.230443
CAACGCA94600.034.9973565
AACGCAG98100.034.184516
TATCAAC96500.034.11142
ACGCAGA108900.030.4889817
CGCAGAG110300.029.9297378
GCAGAGT117950.027.6663869
TACATGG66400.025.3237952
GTACATG67350.025.1076471
GAGTACT82300.024.9620312-13
CAGAGTA116450.024.00493810-11
ACATGGG69200.023.4754333
AGAGTAC109700.022.90565310-11
GTACTTT89200.022.84473214-15
GTGGTAT14650.021.723551
AGTACTT85300.021.18845212-13
CATACGG1150.003724201820.6521745
Produced by FastQC (version 0.11.5)