##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031803_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1960219 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.60417739038342 34.0 31.0 34.0 31.0 34.0 2 32.7292312746688 34.0 31.0 34.0 31.0 34.0 3 32.80451112860349 34.0 33.0 34.0 31.0 34.0 4 36.002976708214746 37.0 37.0 37.0 35.0 37.0 5 36.03733205320426 37.0 37.0 37.0 35.0 37.0 6 36.112145122560285 37.0 37.0 37.0 35.0 37.0 7 36.13410950511142 37.0 37.0 37.0 35.0 37.0 8 36.12944114917772 37.0 37.0 37.0 35.0 37.0 9 37.9619991439732 39.0 39.0 39.0 37.0 39.0 10-11 37.998875635834565 39.0 39.0 39.0 37.0 39.0 12-13 37.97323895952442 39.0 39.0 39.0 37.0 39.0 14-15 39.53300498566742 41.0 40.0 41.0 37.0 41.0 16-17 39.47310275025393 41.0 40.0 41.0 37.0 41.0 18-19 39.49235008945429 41.0 40.0 41.0 37.0 41.0 20-21 39.44137185692007 41.0 40.0 41.0 37.0 41.0 22-23 39.35107225264116 41.0 40.0 41.0 37.0 41.0 24-25 39.31161008030225 41.0 40.0 41.0 36.0 41.0 26-27 39.19107354841474 41.0 39.5 41.0 36.0 41.0 28-29 39.065160066298716 41.0 39.0 41.0 36.0 41.0 30-31 38.88487102716584 41.0 39.0 41.0 35.0 41.0 32-33 38.72784826593355 40.5 38.5 41.0 35.0 41.0 34-35 38.52190622578395 40.0 38.0 41.0 35.0 41.0 36-37 38.38825381245667 40.0 38.0 41.0 34.5 41.0 38-39 38.18912198075827 40.0 38.0 41.0 34.0 41.0 40-41 38.083234577361 40.0 38.0 41.0 34.0 41.0 42-43 37.89506708179035 40.0 38.0 41.0 33.0 41.0 44-45 38.00416535091232 40.0 38.0 41.0 33.0 41.0 46-47 37.817543600995606 40.0 38.0 41.0 33.0 41.0 48-49 37.596605787414575 40.0 38.0 41.0 32.5 41.0 50-51 36.51934732802815 39.0 36.5 40.0 30.5 40.5 52-53 36.531184780884175 39.5 36.5 40.0 30.5 40.5 54-55 37.29371743667417 40.0 37.0 41.0 31.5 41.0 56-57 37.27238997275305 40.0 37.0 41.0 31.5 41.0 58-59 37.13652530661115 40.0 36.5 41.0 31.0 41.0 60-61 36.93909175454375 40.0 36.0 41.0 31.0 41.0 62-63 36.67042611055193 39.0 35.0 41.0 31.0 41.0 64-65 36.34958568404856 39.0 35.0 41.0 30.0 41.0 66-67 36.07127877038229 38.5 35.0 41.0 30.0 41.0 68-69 35.7379229055529 37.5 35.0 40.5 29.5 41.0 70-71 35.37857912814844 37.0 35.0 40.0 29.0 41.0 72-73 34.98249430293248 36.5 35.0 39.0 29.0 41.0 74-75 34.63185924633932 36.0 35.0 39.0 29.0 41.0 76-77 34.26880669965958 35.5 35.0 38.0 28.5 40.0 78-79 33.90695605950151 35.0 34.5 37.0 28.0 39.0 80-81 33.57314871450588 35.0 34.0 37.0 27.0 39.0 82-83 33.29879493056643 35.0 34.0 36.0 27.0 38.5 84-85 33.005584580090286 35.0 34.0 36.0 26.0 37.0 86-87 32.763179012140995 35.0 34.0 36.0 26.0 37.0 88-89 32.57338720826601 35.0 34.0 35.0 25.5 36.5 90-91 32.40935120004448 35.0 34.0 35.0 25.0 36.0 92-93 32.287165107572164 35.0 34.0 35.0 25.0 36.0 94-95 32.125812983141174 35.0 34.0 35.0 24.5 36.0 96-97 32.02908629086852 35.0 34.0 35.0 24.0 36.0 98-99 31.924023030079802 35.0 33.0 35.0 24.0 35.0 100-101 30.436322676190773 34.0 30.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1898405955941982 1101 2 0.19226968517116916 1101 3 0.18188628820108477 1101 4 0.15780202711572144 1101 5 0.15280777374600518 1101 6 0.10107005949856784 1101 7 0.2574927525685382 1101 8 0.2639382046352665 1101 9 0.19820390107727803 1101 10-11 0.25158500630234926 1101 12-13 0.2664874238541941 1101 14-15 0.3366847387950216 1101 16-17 0.33876181204978906 1101 18-19 0.31782377341685475 1101 20-21 0.32099314438193716 1101 22-23 0.41116206771257424 1101 24-25 0.32081428540107737 1101 26-27 0.3104541962556624 1101 28-29 0.47530057502772394 1101 30-31 0.5134231648789296 1101 32-33 0.36584120344003423 1101 34-35 0.5001242482158474 1101 36-37 0.5580632015602589 1101 38-39 0.5708112382561552 1101 40-41 0.5887403624792853 1101 42-43 0.5941073369599152 1101 44-45 0.7254624163005658 1101 46-47 0.7476437128224305 1101 48-49 0.7510266673327379 1101 50-51 0.884976840628731 1101 52-53 0.887365775106062 1101 54-55 0.9185772807086465 1101 56-57 0.9038525007456144 1101 58-59 0.7611042327953115 1101 60-61 0.8163172100721923 1101 62-63 0.757199462022129 1101 64-65 0.8370630348728199 1101 66-67 0.8316622102182478 1101 68-69 0.8573763346279151 1101 70-71 0.7770017567877332 1101 72-73 0.8066290436384946 1101 74-75 0.7711227910977314 1101 76-77 0.6831604986058224 1101 78-79 0.6869876743452394 1101 80-81 0.7356409018133192 1101 82-83 0.6674514204533821 1101 84-85 0.7227822422843246 1101 86-87 0.7742937534740335 1101 88-89 0.8046837020792381 1101 90-91 0.6525334490155288 1101 92-93 0.7114383662815058 1101 94-95 0.7165566677029105 1101 96-97 0.5654677441818023 1101 98-99 0.5465468195154521 1101 100-101 0.6074697944115854 1102 1 0.10694519876807362 1102 2 0.14148772647822483 1102 3 0.15792746414255276 1102 4 0.18018976824760102 1102 5 0.13068797810112898 1102 6 0.1439783085605626 1102 7 0.0985763042984047 1102 8 0.22040868794336888 1102 9 0.16349409785522084 1102 10-11 0.3190192243512797 1102 12-13 0.3109610386560675 1102 14-15 0.3261531090308907 1102 16-17 0.39375943370008315 1102 18-19 0.3920684949288784 1102 20-21 0.37575693309300817 1102 22-23 0.37183144356348663 1102 24-25 0.38225460483173634 1102 26-27 0.43221445708134354 1102 28-29 0.42464001126737827 1102 30-31 0.44296131528515303 1102 32-33 0.40597318660254444 1102 34-35 0.49495152819182664 1102 36-37 0.559409408473627 1102 38-39 0.4964927650346951 1102 40-41 0.4326748846825552 1102 42-43 0.26770209056441274 1102 44-45 0.3410290581165256 1102 46-47 0.39424858051148703 1102 48-49 0.44051100757700823 1102 50-51 0.4848545690608361 1102 52-53 0.5715664819028063 1102 54-55 0.5408673341757719 1102 56-57 0.5827474676392939 1102 58-59 0.49049644248038504 1102 60-61 0.4158473335481787 1102 62-63 0.3140599336976635 1102 64-65 0.37639099100141493 1102 66-67 0.42433857018452414 1102 68-69 0.4148176724801118 1102 70-71 0.36078884417835866 1102 72-73 0.3923719806259456 1102 74-75 0.46280862939739364 1102 76-77 0.4042395375695875 1102 78-79 0.3148891466351742 1102 80-81 0.4053001346125953 1102 82-83 0.4743959347745559 1102 84-85 0.39335698725967916 1102 86-87 0.3497935880567624 1102 88-89 0.4774845290131253 1102 90-91 0.4416233796012605 1102 92-93 0.4978233052295806 1102 94-95 0.3977522305561436 1102 96-97 0.2906551535643942 1102 98-99 0.3298723012456044 1102 100-101 0.2307910378006106 1103 1 0.12873499467792726 1103 2 0.19413675565640176 1103 3 0.1873694240529673 1103 4 0.1959684901019756 1103 5 0.15119604175400525 1103 6 0.19756291887642163 1103 7 0.14145838076026962 1103 8 0.28698508655375576 1103 9 0.299577710727597 1103 10-11 0.2586606868606083 1103 12-13 0.2182055714841269 1103 14-15 0.31852840079179856 1103 16-17 0.2767610504621416 1103 18-19 0.28435745743473007 1103 20-21 0.2741643599112322 1103 22-23 0.4336480905129818 1103 24-25 0.3320454892408975 1103 26-27 0.3623720392505305 1103 28-29 0.3487818275737311 1103 30-31 0.36490840838033733 1103 32-33 0.2774409507582405 1103 34-35 0.4978653143204781 1103 36-37 0.42545691875737646 1103 38-39 0.4541429480499346 1103 40-41 0.3841876623854148 1103 42-43 0.26978907597740687 1103 44-45 0.46995418938286804 1103 46-47 0.49125360873878776 1103 48-49 0.44550309058654847 1103 50-51 0.35195773599603086 1103 52-53 0.49907857981492043 1103 54-55 0.5824315340870783 1103 56-57 0.6390583655608566 1103 58-59 0.5612546315956664 1103 60-61 0.6083831198742402 1103 62-63 0.5437682144943352 1103 64-65 0.5416312323896904 1103 66-67 0.5244671257056339 1103 68-69 0.5864120111716034 1103 70-71 0.5000163155096757 1103 72-73 0.5092025014235091 1103 74-75 0.4180383611372278 1103 76-77 0.4620587646655778 1103 78-79 0.46984442502269275 1103 80-81 0.5536170269262328 1103 82-83 0.4733609568295236 1103 84-85 0.4568235864569914 1103 86-87 0.5576338761545898 1103 88-89 0.5005091391065619 1103 90-91 0.43552820055494124 1103 92-93 0.3798038456979782 1103 94-95 0.49230379108600886 1103 96-97 0.42667725162942105 1103 98-99 0.5001895994874666 1103 100-101 0.5820082663951993 1104 1 0.10620252390359752 1104 2 0.09062179269265869 1104 3 0.12754056073371345 1104 4 0.172938085948239 1104 5 0.07928802410693692 1104 6 0.09610275223296583 1104 7 0.12359518500019107 1104 8 0.1861910207634878 1104 9 0.21017370676514702 1104 10-11 0.15141253735973237 1104 12-13 0.15321048512749513 1104 14-15 0.19814873644357078 1104 16-17 0.30908007992560727 1104 18-19 0.2862999231377614 1104 20-21 0.32852515271576976 1104 22-23 0.2541486909234081 1104 24-25 0.2908526694665099 1104 26-27 0.3404291399907322 1104 28-29 0.293757561532793 1104 30-31 0.2757665091914916 1104 32-33 0.18801592586157767 1104 34-35 0.2723402507978463 1104 36-37 0.2078330988857573 1104 38-39 0.24234291231434923 1104 40-41 0.23344785688161096 1104 42-43 0.28207975345584657 1104 44-45 0.25901929647138644 1104 46-47 0.40753445367523966 1104 48-49 0.31869299107869864 1104 50-51 0.16039691849190518 1104 52-53 0.30433177696762925 1104 54-55 0.23154681551798006 1104 56-57 0.3045611074789676 1104 58-59 0.11145032335519289 1104 60-61 0.1423260165064235 1104 62-63 0.2117862425730479 1104 64-65 0.3230634411906337 1104 66-67 0.3440368928725164 1104 68-69 0.31928980334288326 1104 70-71 0.22161177814103183 1104 72-73 0.20397328127389613 1104 74-75 0.24753690675133555 1104 76-77 0.320171728538476 1104 78-79 0.1894990642114962 1104 80-81 0.20977596358227402 1104 82-83 0.24847111966293056 1104 84-85 0.2167678021528019 1104 86-87 0.19531212710775492 1104 88-89 0.2445529887753466 1104 90-91 0.23491294408640329 1104 92-93 0.3110719598311462 1104 94-95 0.30451294675390983 1104 96-97 0.20061812499668719 1104 98-99 0.1443613484029136 1104 100-101 0.27318118197249674 1105 1 0.15010777116671647 1105 2 0.12078537643495224 1105 3 0.15781928226385133 1105 4 0.19957895671708314 1105 5 0.18882558545083583 1105 6 0.20219813413808652 1105 7 0.15773448284843283 1105 8 0.23954889165816695 1105 9 0.24606649649712153 1105 10-11 0.2539726229266108 1105 12-13 0.19198360706030115 1105 14-15 0.23879457764150658 1105 16-17 0.3078818290133185 1105 18-19 0.33238272167809413 1105 20-21 0.264826901803481 1105 22-23 0.2701336538024819 1105 24-25 0.31264422602873054 1105 26-27 0.36976505291384854 1105 28-29 0.3997853078521558 1105 30-31 0.36241807582040764 1105 32-33 0.40550617375386366 1105 34-35 0.4211844687417212 1105 36-37 0.5050521752667834 1105 38-39 0.446392239104334 1105 40-41 0.42958853889997783 1105 42-43 0.5201145464594958 1105 44-45 0.4817155969621396 1105 46-47 0.5299910410667081 1105 48-49 0.45209112874071167 1105 50-51 0.3904560603064269 1105 52-53 0.4698042823155646 1105 54-55 0.41967566917344357 1105 56-57 0.4460704820857373 1105 58-59 0.3930147501829282 1105 60-61 0.3959185671544603 1105 62-63 0.3526532364071997 1105 64-65 0.45026490086095095 1105 66-67 0.3412974943573488 1105 68-69 0.3242020343734566 1105 70-71 0.31087156170715247 1105 72-73 0.37182624465630454 1105 74-75 0.3041359378322497 1105 76-77 0.2462054519561221 1105 78-79 0.2890529839296292 1105 80-81 0.3224666015588653 1105 82-83 0.3068025654915445 1105 84-85 0.33943830674572695 1105 86-87 0.45024795194731837 1105 88-89 0.40101957994182413 1105 90-91 0.25882479761604316 1105 92-93 0.3361012161542831 1105 94-95 0.29813003496330026 1105 96-97 0.3469054032148833 1105 98-99 0.40591916896413593 1105 100-101 0.20811228805025905 1106 1 0.2610183142977007 1106 2 0.158481507070384 1106 3 0.229216158373184 1106 4 0.26121722437770956 1106 5 0.23694592623890998 1106 6 0.1858545061650716 1106 7 0.19523714525524838 1106 8 0.16468733681258385 1106 9 0.20785563387731543 1106 10-11 0.23391759985241833 1106 12-13 0.25741491696445706 1106 14-15 0.3039747338360357 1106 16-17 0.36698637285427793 1106 18-19 0.3369372122709251 1106 20-21 0.40240588653900744 1106 22-23 0.36505466906695005 1106 24-25 0.3729202288686295 1106 26-27 0.41344361166428456 1106 28-29 0.38136943990753736 1106 30-31 0.3984244655967615 1106 32-33 0.3761859749608192 1106 34-35 0.38301797956884087 1106 36-37 0.5060283910992354 1106 38-39 0.5122850328565463 1106 40-41 0.4855771792975645 1106 42-43 0.36284173850776824 1106 44-45 0.3146256076117666 1106 46-47 0.425345141173203 1106 48-49 0.31372939640134234 1106 50-51 0.25331371028867267 1106 52-53 0.370989940817509 1106 54-55 0.490172651779055 1106 56-57 0.41427247001615797 1106 58-59 0.3817376823803045 1106 60-61 0.3929721702783482 1106 62-63 0.38906786189530607 1106 64-65 0.3764026360402184 1106 66-67 0.367822165993843 1106 68-69 0.3722451653638785 1106 70-71 0.34492463234968085 1106 72-73 0.38080654284586757 1106 74-75 0.37479000243998684 1106 76-77 0.3893222428684382 1106 78-79 0.33290992393673235 1106 80-81 0.4476609572564172 1106 82-83 0.40028499360656866 1106 84-85 0.4602272666321312 1106 86-87 0.4514992831507314 1106 88-89 0.5050877375563161 1106 90-91 0.5274430084786559 1106 92-93 0.527711972277821 1106 94-95 0.46363411732041726 1106 96-97 0.3248979484757939 1106 98-99 0.4941868295960212 1106 100-101 0.34846638815675846 1107 1 0.13812957802029757 1107 2 0.2260917108067062 1107 3 0.17066974955356784 1107 4 0.12664541569942855 1107 5 0.2113022209648534 1107 6 0.1912324020508649 1107 7 0.18852887537180152 1107 8 0.10298087504342135 1107 9 0.15109245703710172 1107 10-11 0.1329393934354357 1107 12-13 0.1519836070603091 1107 14-15 0.1348355952946676 1107 16-17 0.21925514157820913 1107 18-19 0.22606911835514154 1107 20-21 0.27530457573909217 1107 22-23 0.2263200504795364 1107 24-25 0.24447444409726415 1107 26-27 0.2043003592482151 1107 28-29 0.24482944077012547 1107 30-31 0.18694559399278177 1107 32-33 0.21785819867597667 1107 34-35 0.30972548639488906 1107 36-37 0.3651248647787284 1107 38-39 0.4595594249817978 1107 40-41 0.4496612284119266 1107 42-43 0.5014618984844148 1107 44-45 0.4560068742207122 1107 46-47 0.4495523079410617 1107 48-49 0.5246923748466301 1107 50-51 0.4985869014279203 1107 52-53 0.46716669145367007 1107 54-55 0.4726455549470643 1107 56-57 0.3970829431449303 1107 58-59 0.48238910116943146 1107 60-61 0.5177254207370154 1107 62-63 0.3714694357841495 1107 64-65 0.45781422588690646 1107 66-67 0.6243504538589022 1107 68-69 0.5606116916943336 1107 70-71 0.5345709386541913 1107 72-73 0.5624830463177801 1107 74-75 0.5442891050181302 1107 76-77 0.45128333357605044 1107 78-79 0.43599483232007685 1107 80-81 0.44705382897320334 1107 82-83 0.2764391179318366 1107 84-85 0.34331940747262024 1107 86-87 0.46553870999508007 1107 88-89 0.41191262251711436 1107 90-91 0.3462624923200863 1107 92-93 0.2890425453339276 1107 94-95 0.4035557878189593 1107 96-97 0.3953944997880896 1107 98-99 0.4296471025051609 1107 100-101 0.4858615092340628 1108 1 0.06079882807728154 1108 2 0.14115800516124466 1108 3 0.05636264269745084 1108 4 0.18571039303144232 1108 5 0.25321582935327314 1108 6 0.2344677818345673 1108 7 0.2136694421980252 1108 8 0.2014188103573531 1108 9 0.2554364151963142 1108 10-11 0.12982966899706838 1108 12-13 0.1385181599058356 1108 14-15 0.1671651738474651 1108 16-17 0.2200125878209036 1108 18-19 0.20491998455072036 1108 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0.31833397070236913 1108 86-87 0.22722965926438832 1108 88-89 0.3101354336003652 1108 90-91 0.2115890252583199 1108 92-93 0.1701052811949353 1108 94-95 0.15868220298498414 1108 96-97 0.26713914305227604 1108 98-99 0.28867662153865936 1108 100-101 0.16073423926977526 1109 1 0.1338672315155094 1109 2 0.1032017414673092 1109 3 0.08286315634523334 1109 4 0.206558774639646 1109 5 0.19579662855711888 1109 6 0.17981796522287397 1109 7 0.11124386093332816 1109 8 0.1538973615495749 1109 9 0.2005317811302305 1109 10-11 0.16356514239573983 1109 12-13 0.20002572617843128 1109 14-15 0.18314167646786927 1109 16-17 0.23957509434182356 1109 18-19 0.25026470039915694 1109 20-21 0.3254071365298117 1109 22-23 0.3319020534953623 1109 24-25 0.34942140053788506 1109 26-27 0.3058232409142789 1109 28-29 0.2644472032124696 1109 30-31 0.43186855295103754 1109 32-33 0.42880385939607635 1109 34-35 0.46530369655788917 1109 36-37 0.352315529440304 1109 38-39 0.3468386578824365 1109 40-41 0.3636644410607772 1109 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84-85 -0.3820691316550153 2316 86-87 -0.3059933311768148 2316 88-89 -0.27626455851385856 2316 90-91 -0.32299687095249396 2316 92-93 -0.6523681004426507 2316 94-95 -0.8038341677331218 2316 96-97 -0.6945482547032142 2316 98-99 -0.6055892203685183 2316 100-101 -0.2540212861858251 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 22.0 7 351.0 8 737.0 9 728.0 10 849.0 11 1281.0 12 1696.0 13 2135.0 14 2461.0 15 2630.0 16 3158.0 17 4185.0 18 5402.0 19 7496.0 20 10162.0 21 13734.0 22 17236.0 23 18249.0 24 16814.0 25 14352.0 26 12717.0 27 12782.0 28 13932.0 29 15623.0 30 19042.0 31 23603.0 32 29321.0 33 40001.0 34 59727.0 35 99753.0 36 195834.0 37 432456.0 38 655297.0 39 226069.0 40 384.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.13705815523674 18.998999601575132 13.933494165702914 22.93044807748522 2 15.08775295005303 20.665752143000347 40.65724288969753 23.589252017249095 3 17.61486854274956 26.15126167025215 31.420468835369924 24.813400951628363 4 11.938155889724566 17.585688129744685 39.596443050495886 30.87971293003486 5 12.633333316328429 38.43254248632423 35.51266465634707 13.421459541000266 6 29.767541279826386 38.70506305672989 18.090325621780014 13.437070041663713 7 26.278696410962244 33.974673238041255 22.450705762978522 17.29592458801797 8 25.8207373767931 34.86130886395857 20.589536169172934 18.728417590075395 9 26.551523069616202 15.34910129939563 20.396700572742127 37.702675058246044 10-11 23.931866796516104 26.23635420328035 29.537592483288854 20.294186516914692 12-13 25.279343787607406 24.377556793399105 29.0297920793544 21.313307339639092 14-15 22.450272137960095 25.554823211079984 27.22241749518804 24.77248715577188 16-17 21.42309099136372 28.71120522757916 27.675479117384334 22.190224663672783 18-19 21.243850814628367 27.44843815920568 30.25238506513813 21.055325961027823 20-21 22.397726988668104 26.5782037619266 30.29809424355136 20.72597500585394 22-23 21.97615265240907 26.579773270115876 29.96758523796843 21.476488839506626 24-25 21.667393627739813 26.70398400175491 30.14465249985333 21.483969870651944 26-27 21.625593874970093 26.900310628557317 29.96999314872471 21.50410234774788 28-29 21.646433383208713 26.951019248359493 30.041056637039027 21.361490731392767 30-31 21.84758437705175 26.80996358059992 29.988205399498728 21.3542466428496 32-33 21.614855278925468 26.946682998175202 29.86577010017758 21.572691622721745 34-35 21.954842800727878 27.083938070185017 29.695967644431565 21.265251484655543 36-37 21.93150357179478 26.913472423234342 29.657272988375276 21.497751016595597 38-39 21.87097462069289 27.144441513932883 29.552718344225827 21.431865521148403 40-41 22.00374039839426 27.050446914349873 29.54705571163222 21.39875697562364 42-43 21.90324142353482 27.162857823539106 29.36291812292402 21.570982630002057 44-45 22.081843916419544 27.252796753832097 29.126898576128486 21.538460753619876 46-47 22.10003065983954 27.29485838061972 28.76464313426204 21.840467825278704 48-49 21.998996030545566 27.57498014252489 28.62514341509801 21.800880411831535 50-51 22.1094938881829 27.798909203512466 28.324156637600183 21.767440270704448 52-53 22.173389810016126 27.82385539574915 28.26174014230043 21.7410146519343 54-55 22.125078881492325 27.806102277347584 28.274315267834872 21.794503573325226 56-57 22.132705580345867 27.94208198165613 28.13287698976492 21.79233544823308 58-59 22.036619377732794 27.70945491294595 28.303597710255847 21.95032799906541 60-61 22.09219990215379 27.783477254327195 28.417232972438285 21.707089871080733 62-63 21.97068286757755 27.791512070845148 28.456106179972746 21.781698881604555 64-65 21.943389998770545 27.674331286453196 28.45266268717934 21.929616027596914 66-67 21.927192828964518 27.81408607915748 28.286252709518685 21.972468382359317 68-69 22.02259033301891 27.705960405444497 28.234319736723297 22.0371295248133 70-71 22.00062850120318 27.605844040895434 28.217127780110285 22.176399677791103 72-73 22.001954883612495 27.74052287014869 28.141779056319727 22.115743189919083 74-75 21.938569108859777 27.864437596003306 28.029674235378803 22.16731905975812 76-77 21.92632557892766 27.805745174391227 28.04324414772023 22.22468509896088 78-79 21.937650844114867 27.847220132036266 27.909228509671625 22.305900514177242 80-81 21.994812824485425 27.944224599394254 27.757536275283528 22.303426300836797 82-83 22.019733509368088 27.888899148513506 27.817274498410637 22.27409284370777 84-85 22.052663503414667 27.78370682051342 27.857040463335984 22.306589212735926 86-87 21.99516992744178 27.788094085405763 27.814927821840314 22.40180816531214 88-89 21.958821947955816 27.74315012761329 27.79635846810994 22.501669456320954 90-91 22.00572997200823 27.627040652090407 27.90019890634669 22.46703046955468 92-93 22.01006622219252 27.61120058524073 27.930272076742447 22.4484611158243 94-95 21.918061196223483 27.575949421977853 27.99855526346801 22.507434118330654 96-97 21.922320924345698 27.462033578901135 28.054773471739637 22.56087202501353 98-99 21.90023155575984 27.498407065741127 27.839159808164293 22.762201570334742 100-101 22.08860336523623 27.346128162210448 27.949147518721123 22.6161209538322 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 217.0 1 197.0 2 284.0 3 432.5 4 400.5 5 426.0 6 690.5 7 1344.5 8 2471.5 9 3169.5 10 2787.5 11 2340.0 12 2423.0 13 2580.0 14 2760.0 15 2971.0 16 3153.0 17 3419.5 18 3744.5 19 4118.5 20 4596.5 21 5252.5 22 6392.5 23 7800.5 24 9274.0 25 11291.0 26 14143.0 27 17650.5 28 20972.0 29 24160.0 30 27502.5 31 30875.5 32 34547.5 33 38020.0 34 41203.5 35 43972.5 36 47645.5 37 51669.5 38 54477.0 39 56768.0 40 59392.5 41 62234.0 42 63840.0 43 65778.0 44 68376.5 45 71084.0 46 73074.0 47 73534.5 48 73748.5 49 73558.5 50 71660.0 51 68774.0 52 66290.0 53 64091.0 54 61228.0 55 56522.5 56 50611.0 57 44540.0 58 40165.5 59 35595.5 60 29619.5 61 24143.0 62 19080.5 63 14370.5 64 10307.0 65 7463.0 66 5595.5 67 4289.0 68 3287.0 69 2528.5 70 1916.0 71 1434.0 72 1030.0 73 760.0 74 616.0 75 454.5 76 325.5 77 217.0 78 152.0 79 107.0 80 75.0 81 57.0 82 49.5 83 42.0 84 30.5 85 20.0 86 18.0 87 19.5 88 19.0 89 11.0 90 7.5 91 7.5 92 8.0 93 9.0 94 5.0 95 3.5 96 2.5 97 3.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.101470805047804E-5 24-25 2.0405883220191216E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1960219.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.46633206626725 #Duplication Level Percentage of deduplicated Percentage of total 1 73.45437007634558 44.415163327547965 2 15.337345430093146 18.54786043582117 3 5.2913478849865605 9.598451948752148 4 2.342884007542654 5.6666240957128435 5 1.163835066602199 3.518641880376741 6 0.6857141007156797 2.4877569909837667 7 0.43368289362205154 1.8356249700047431 8 0.2696160764311846 1.3042156166313679 9 0.19115599590277602 1.0402651732243746 >10 0.7921587634427358 7.965077845404004 >50 0.02661506250266093 1.0738675070266979 >100 0.01042748738996085 1.152738892595514 >500 4.235772113798841E-4 0.15008725946933668 >1k 2.5414632682792075E-4 0.35114271797347035 >5k 1.6943088455194716E-4 0.8924813384758239 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9606 0.490047285532892 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7817 0.3987819728305868 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4660 0.23772853951522763 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.5507354025239017E-5 0.0 14-15 0.0 0.0 0.0 5.1014708050478034E-5 0.0 16-17 0.0 0.0 0.0 2.0405883220191214E-4 0.0 18-19 0.0 0.0 0.0 3.060882483028682E-4 0.0 20-21 5.1014708050478034E-5 0.0 0.0 3.060882483028682E-4 0.0 22-23 5.1014708050478034E-5 0.0 0.0 5.611617885552583E-4 0.0 24-25 5.1014708050478034E-5 0.0 0.0 0.0011223235771105167 0.0 26-27 5.1014708050478034E-5 0.0 0.0 0.0018110221357919704 0.0 28-29 5.1014708050478034E-5 0.0 0.0 0.0035200148554829845 0.0 30-31 5.1014708050478034E-5 0.0 0.0 0.007830757685748379 0.0 32-33 5.1014708050478034E-5 0.0 0.0 0.014182088838032894 0.0 34-35 5.1014708050478034E-5 0.0 0.0 0.021910817107680315 0.0 36-37 5.1014708050478034E-5 0.0 0.0 0.03599087652961225 0.0 38-39 5.1014708050478034E-5 0.0 0.0 0.056702847998106334 0.0 40-41 5.1014708050478034E-5 0.0 0.0 0.08310295941422871 0.0 42-43 5.1014708050478034E-5 0.0 0.0 0.11177322533859738 0.0 44-45 5.1014708050478034E-5 0.0 0.0 0.14613163121059433 0.0 46-47 5.1014708050478034E-5 0.0 0.0 0.19150921402149454 0.0 48-49 1.0202941610095607E-4 0.0 0.0 0.24448798833191598 0.0 50-51 1.0202941610095607E-4 0.0 0.0 0.2968290787917064 0.0 52-53 1.0202941610095607E-4 0.0 0.0 0.35121075757351605 0.0 54-55 1.0202941610095607E-4 0.0 0.0 0.41143362042710535 0.0 56-57 1.0202941610095607E-4 0.0 0.0 0.48805771191892333 0.0 58-59 1.0202941610095607E-4 0.0 0.0 0.5769508406968813 0.0 60-61 1.0202941610095607E-4 0.0 0.0 0.6760214037309096 0.0 62-63 1.0202941610095607E-4 0.0 0.0 0.7795047390113043 0.0 64-65 1.0202941610095607E-4 0.0 0.0 0.8907678172693969 0.0 66-67 1.0202941610095607E-4 0.0 0.0 1.031109279116262 0.0 68-69 1.0202941610095607E-4 0.0 0.0 1.1997639039311423 0.0 70-71 1.530441241514341E-4 0.0 0.0 1.3841820735336205 0.0 72-73 1.530441241514341E-4 0.0 0.0 1.583062912868409 0.0 74-75 1.530441241514341E-4 0.0 0.0 1.7770208328763266 0.0 76-77 1.530441241514341E-4 0.0 0.0 1.9858240329269332 0.0 78-79 1.530441241514341E-4 0.0 0.0 2.2267409916953156 0.0 80-81 1.530441241514341E-4 0.0 0.0 2.4986238782503385 0.0 82-83 1.530441241514341E-4 0.0 0.0 2.7888975670575586 5.1014708050478034E-5 84-85 1.530441241514341E-4 0.0 0.0 3.085981719389517 5.1014708050478034E-5 86-87 1.530441241514341E-4 5.1014708050478034E-5 0.0 3.4210208145110315 5.1014708050478034E-5 88-89 1.530441241514341E-4 5.1014708050478034E-5 0.0 3.791999771454108 5.1014708050478034E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3920 0.0 66.88776 1 GTATCAA 7475 0.0 44.48161 1 TCAACGC 9265 0.0 35.4776 4 ATCAACG 9330 0.0 35.23044 3 CAACGCA 9460 0.0 34.997356 5 AACGCAG 9810 0.0 34.18451 6 TATCAAC 9650 0.0 34.1114 2 ACGCAGA 10890 0.0 30.488981 7 CGCAGAG 11030 0.0 29.929737 8 GCAGAGT 11795 0.0 27.666386 9 TACATGG 6640 0.0 25.323795 2 GTACATG 6735 0.0 25.107647 1 GAGTACT 8230 0.0 24.96203 12-13 CAGAGTA 11645 0.0 24.004938 10-11 ACATGGG 6920 0.0 23.475433 3 AGAGTAC 10970 0.0 22.905653 10-11 GTACTTT 8920 0.0 22.844732 14-15 GTGGTAT 1465 0.0 21.72355 1 AGTACTT 8530 0.0 21.188452 12-13 CATACGG 115 0.0037242018 20.652174 5 >>END_MODULE