FastQCFastQC Report
Fri 10 Feb 2017
SRR2031803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960219
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116730.5954946870732301No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98320.5015766095523001No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62730.3200152636006487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39650.069.1239551
GTATCAA79300.046.4426571
TCAACGC102300.035.6126254
TATCAAC102350.035.4560052
ATCAACG102700.035.427673
CAACGCA103250.035.2849585
AACGCAG106200.034.4837236
ACGCAGA119450.030.4995567
CGCAGAG120350.030.153078
GCAGAGT131300.027.3851689
TACATGG61250.026.2116812
GTACATG62850.026.1193981
TGGTATC14100.025.2654132
GTGGTAT14450.025.0262091
ACATGGG63350.024.7429563
GAGTACT96350.023.90970812-13
CAGAGTA129450.023.59354410-11
AGAGTAC122300.022.9533110-11
AGTACTT100550.021.91897212-13
GTACTTT104100.021.90154614-15
Produced by FastQC (version 0.11.5)