FastQCFastQC Report
Fri 10 Feb 2017
SRR2031802_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031802_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236123
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67140.3002518197791445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55870.24985208774293724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35150.15719171083164923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44700.043.8870241
GTATCAA71050.036.1013371
TCAACGC83550.029.9611024
CAACGCA84700.029.610395
ATCAACG86000.029.2180233
AACGCAG89350.028.707336
TATCAAC88600.028.414222
ACGCAGA97700.026.1566037
CGCAGAG98750.025.7822788
GTACATG61600.024.5982151
GTATAGG7800.024.3589731
TACATGG62950.023.5424942
GAGTACT65750.022.9011412-13
ACATGGG63300.022.5868853
GCAGAGT111100.022.5742579
AGAGTAC89950.022.02056710-11
GTACTTT71050.020.9922614-15
CAGAGTA108000.020.12152710-11
AGTACTT70900.019.59626412-13
GTATAGC9450.019.1005291
Produced by FastQC (version 0.11.5)