##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031802_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2236123 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.357749551344 34.0 31.0 34.0 30.0 34.0 2 32.49034780287131 34.0 31.0 34.0 31.0 34.0 3 32.564768574895034 34.0 31.0 34.0 31.0 34.0 4 35.77805872038345 37.0 35.0 37.0 35.0 37.0 5 35.801172386313276 37.0 37.0 37.0 35.0 37.0 6 35.87391838463269 37.0 37.0 37.0 35.0 37.0 7 35.893160170527295 37.0 37.0 37.0 35.0 37.0 8 35.88517044903165 37.0 37.0 37.0 35.0 37.0 9 37.68211542924964 39.0 39.0 39.0 35.0 39.0 10-11 37.705807998933864 39.0 39.0 39.0 35.0 39.0 12-13 37.67090987392017 39.0 39.0 39.0 35.0 39.0 14-15 39.194978317382365 41.0 40.0 41.0 36.0 41.0 16-17 39.13044541825293 41.0 40.0 41.0 36.0 41.0 18-19 39.129732130119855 41.0 40.0 41.0 36.0 41.0 20-21 39.071118851691075 41.0 39.5 41.0 36.0 41.0 22-23 38.97959615817198 41.0 39.0 41.0 35.5 41.0 24-25 38.93548722498717 41.0 39.0 41.0 35.0 41.0 26-27 38.80578125621891 41.0 39.0 41.0 35.0 41.0 28-29 38.68479238396099 41.0 39.0 41.0 35.0 41.0 30-31 38.52530943065297 40.0 38.5 41.0 34.0 41.0 32-33 38.39346762230879 40.0 38.0 41.0 34.0 41.0 34-35 38.19578328204665 40.0 38.0 41.0 33.5 41.0 36-37 38.07036777493904 40.0 38.0 41.0 33.0 41.0 38-39 37.884877754935665 40.0 38.0 41.0 33.0 41.0 40-41 37.78597420624894 40.0 38.0 41.0 33.0 41.0 42-43 37.61402570431054 40.0 38.0 41.0 32.0 41.0 44-45 37.7742548151421 40.0 38.0 41.0 33.0 41.0 46-47 37.643046469268455 40.0 38.0 41.0 32.0 41.0 48-49 37.46115888973907 40.0 38.0 41.0 31.0 41.0 50-51 36.4009258435247 39.0 36.0 40.0 29.5 40.5 52-53 36.43567393206904 39.0 36.5 40.0 30.0 40.5 54-55 37.214748696739846 40.0 37.0 41.0 31.0 41.0 56-57 37.20691885017058 40.0 37.0 41.0 31.0 41.0 58-59 37.07847578152007 40.0 36.0 41.0 31.0 41.0 60-61 36.876234223251586 40.0 36.0 41.0 30.5 41.0 62-63 36.602490560671306 39.0 35.0 41.0 30.0 41.0 64-65 36.28532777490326 39.0 35.0 41.0 29.0 41.0 66-67 36.00455788881023 38.5 35.0 41.0 29.0 41.0 68-69 35.675761798434166 37.5 35.0 40.5 29.0 41.0 70-71 35.32457069669245 37.0 35.0 40.0 29.0 41.0 72-73 34.93151047594431 36.5 35.0 39.0 28.0 41.0 74-75 34.583945963616486 36.0 35.0 39.0 28.0 41.0 76-77 34.221137880161336 35.5 34.0 38.0 28.0 40.0 78-79 33.85975436056067 35.0 34.0 37.0 27.0 39.0 80-81 33.521721971465794 35.0 34.0 37.0 26.0 39.0 82-83 33.245193131147076 35.0 34.0 36.0 26.0 38.5 84-85 32.95881308854656 35.0 34.0 36.0 26.0 37.0 86-87 32.710707774125126 35.0 34.0 36.0 25.5 37.0 88-89 32.51574555603605 35.0 34.0 35.0 25.0 36.5 90-91 32.35311004806086 35.0 33.5 35.0 25.0 36.0 92-93 32.228751727879015 35.0 33.0 35.0 25.0 36.0 94-95 32.07191308349317 35.0 33.0 35.0 24.5 36.0 96-97 31.96661028932666 35.0 33.0 35.0 24.0 35.5 98-99 31.85948738061368 35.0 33.0 35.0 24.0 35.0 100-101 30.348343315640506 34.0 30.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28696313524840633 1101 2 0.32887683797694933 1101 3 0.3616219711865014 1101 4 0.3441221832870198 1101 5 0.316632619256616 1101 6 0.3430055347293006 1101 7 0.3593826429161524 1101 8 0.4109153056941679 1101 9 0.43148542890772035 1101 10-11 0.4306583561471129 1101 12-13 0.4750681608088101 1101 14-15 0.4647033924079551 1101 16-17 0.5128703576938491 1101 18-19 0.5945056034013447 1101 20-21 0.6402979537104443 1101 22-23 0.6012330662367731 1101 24-25 0.5673818792246834 1101 26-27 0.5590319888215802 1101 28-29 0.592492966908722 1101 30-31 0.544548215586893 1101 32-33 0.33722764678514494 1101 34-35 0.49319732353594503 1101 36-37 0.5726725033079063 1101 38-39 0.6063735331067264 1101 40-41 0.5179636458659331 1101 42-43 0.37443952701575256 1101 44-45 0.5180917903924112 1101 46-47 0.5535668094592197 1101 48-49 0.4293042471132793 1101 50-51 0.5277354213807257 1101 52-53 0.4140616148032308 1101 54-55 0.4765602857597955 1101 56-57 0.5004393025349003 1101 58-59 0.37576292782930665 1101 60-61 0.4250860340117484 1101 62-63 0.2794144759417776 1101 64-65 0.38804489257978503 1101 66-67 0.42603843661890295 1101 68-69 0.34898109655932075 1101 70-71 0.2753984651407535 1101 72-73 0.3157546191350207 1101 74-75 0.43711660877482217 1101 76-77 0.3292308558238659 1101 78-79 0.4004171982225486 1101 80-81 0.5182609253310275 1101 82-83 0.44196659070853883 1101 84-85 0.4314561050519572 1101 86-87 0.507566075109537 1101 88-89 0.33715954321774433 1101 90-91 0.3431212042272591 1101 92-93 0.31826167759415114 1101 94-95 0.3644665321182359 1101 96-97 0.3522776183260703 1101 98-99 0.3077031985975758 1101 100-101 0.2099871065899599 1102 1 0.0018128048853114365 1102 2 0.10448081984571189 1102 3 0.17284943341869763 1102 4 0.17641829099683548 1102 5 0.1669951430824952 1102 6 0.1284488476548873 1102 7 0.11848362423935299 1102 8 0.24225332674850364 1102 9 0.27879268342537245 1102 10-11 0.30551860009719434 1102 12-13 0.33943828197561743 1102 14-15 0.337530859354807 1102 16-17 0.39157389301676915 1102 18-19 0.4108842547386189 1102 20-21 0.4396932983185593 1102 22-23 0.31014226184809957 1102 24-25 0.3288886009855432 1102 26-27 0.4397725106673036 1102 28-29 0.41351976976176985 1102 30-31 0.3291748817147422 1102 32-33 0.319486205580354 1102 34-35 0.33979990447658537 1102 36-37 0.2451234708238914 1102 38-39 0.3672951316465287 1102 40-41 0.3573389026466316 1102 42-43 0.30155253746209354 1102 44-45 0.456060730873169 1102 46-47 0.4492570191269749 1102 48-49 0.3372163759178406 1102 50-51 0.31861490788669045 1102 52-53 0.43602941366228265 1102 54-55 0.43980023511085165 1102 56-57 0.5188146973878389 1102 58-59 0.5555397222633118 1102 60-61 0.55312657000146 1102 62-63 0.3568683732833762 1102 64-65 0.49392104514624435 1102 66-67 0.4045496810875946 1102 68-69 0.38045777918848245 1102 70-71 0.4893746594656534 1102 72-73 0.47856999634165476 1102 74-75 0.46413414731718206 1102 76-77 0.27014982762344886 1102 78-79 0.3052441701299031 1102 80-81 0.2903924745015871 1102 82-83 0.32861433137519214 1102 84-85 0.4201598592699014 1102 86-87 0.4897383387534262 1102 88-89 0.44722164205052906 1102 90-91 0.3074905676805457 1102 92-93 0.310183609527904 1102 94-95 0.32220716509527136 1102 96-97 0.3317423189787192 1102 98-99 0.45508984465443447 1102 100-101 0.23724795536380938 1103 1 0.2148105770936084 1103 2 0.2544313133634546 1103 3 0.22845681607286394 1103 4 0.2870621846816661 1103 5 0.171440670481239 1103 6 0.17959291151824885 1103 7 0.20063963154156284 1103 8 0.2626329795842963 1103 9 0.29185156519771027 1103 10-11 0.3447656374436363 1103 12-13 0.33001116479996284 1103 14-15 0.35523290768551163 1103 16-17 0.3801301602280063 1103 18-19 0.3078113719142763 1103 20-21 0.32087542029015736 1103 22-23 0.3466432365069707 1103 24-25 0.33596454702347245 1103 26-27 0.4360436669530756 1103 28-29 0.41473925365668407 1103 30-31 0.35258139181716075 1103 32-33 0.42070762332223666 1103 34-35 0.4385939575104061 1103 36-37 0.4701555726834741 1103 38-39 0.43760709983861545 1103 40-41 0.3959187627908136 1103 42-43 0.3995985784905898 1103 44-45 0.44741140694607395 1103 46-47 0.45372227176473956 1103 48-49 0.45288324370459776 1103 50-51 0.3347308149382755 1103 52-53 0.3681846474257 1103 54-55 0.4517289767179591 1103 56-57 0.45247446408853875 1103 58-59 0.4515977686137518 1103 60-61 0.5852117985048153 1103 62-63 0.6982561792179709 1103 64-65 0.6484385116520102 1103 66-67 0.6057471191271375 1103 68-69 0.5408228121412364 1103 70-71 0.5330204251143442 1103 72-73 0.6035580064904806 1103 74-75 0.6045987347116011 1103 76-77 0.5630506754219553 1103 78-79 0.6027556958578018 1103 80-81 0.5988174721809685 1103 82-83 0.539612505823662 1103 84-85 0.687368389855628 1103 86-87 0.6173339481472908 1103 88-89 0.530641611727809 1103 90-91 0.5150190339078478 1103 92-93 0.4115489054838477 1103 94-95 0.47486117666741023 1103 96-97 0.5452842757224303 1103 98-99 0.5174784550693623 1103 100-101 0.45898439522890655 1104 1 0.1592247466997989 1104 2 0.023579943999791908 1104 3 0.14441517426020312 1104 4 0.15507231775185204 1104 5 0.16050539890366622 1104 6 0.20446213649056943 1104 7 0.14246358417534566 1104 8 0.2339130406576473 1104 9 0.2876569454455833 1104 10-11 0.1910757074276077 1104 12-13 0.18229135106386707 1104 14-15 0.2087156596192159 1104 16-17 0.3394876627011385 1104 18-19 0.3117669022680758 1104 20-21 0.28993543565668745 1104 22-23 0.2352474600923813 1104 24-25 0.272824939925421 1104 26-27 0.30526360710708644 1104 28-29 0.33394016975692864 1104 30-31 0.2897643344823422 1104 32-33 0.22249171135819523 1104 34-35 0.27911089628241115 1104 36-37 0.33784327872624687 1104 38-39 0.3835920426068924 1104 40-41 0.260012640973045 1104 42-43 0.3422563983585789 1104 44-45 0.32490779237586054 1104 46-47 0.4184001270297344 1104 48-49 0.37907777017748145 1104 50-51 0.27550378065324566 1104 52-53 0.4071810119903958 1104 54-55 0.402324368232982 1104 56-57 0.3924518561485115 1104 58-59 0.2027215378802154 1104 60-61 0.25216713984543304 1104 62-63 0.3175492699271132 1104 64-65 0.373616171767047 1104 66-67 0.35270327052423767 1104 68-69 0.3872510767302302 1104 70-71 0.391514849225544 1104 72-73 0.3086856640280544 1104 74-75 0.19643937941836498 1104 76-77 0.2923747201076239 1104 78-79 0.28735492654961803 1104 80-81 0.18734731785293945 1104 82-83 0.28228579040631985 1104 84-85 0.3224033327466387 1104 86-87 0.3487598439347437 1104 88-89 0.2845825365177461 1104 90-91 0.24008635451102123 1104 92-93 0.20552294440091856 1104 94-95 0.3414970366405612 1104 96-97 0.27564187883893254 1104 98-99 0.2385787081506443 1104 100-101 0.20261919230975778 1105 1 0.21367114666443854 1105 2 0.15690352846674216 1105 3 0.23551550937356325 1105 4 0.24843080065270584 1105 5 0.2786814079466069 1105 6 0.28429970971873786 1105 7 0.2511191669138384 1105 8 0.28654633955647313 1105 9 0.45408937287099604 1105 10-11 0.31998585312928185 1105 12-13 0.30888104713903886 1105 14-15 0.4407931837958543 1105 16-17 0.46409463213750257 1105 18-19 0.4432317760210367 1105 20-21 0.5147519743733966 1105 22-23 0.5676790232854856 1105 24-25 0.49612190152350166 1105 26-27 0.5035848194021284 1105 28-29 0.4809768591923742 1105 30-31 0.48966401676647564 1105 32-33 0.5523395114920149 1105 34-35 0.6595930466879807 1105 36-37 0.6701893826043417 1105 38-39 0.6357226479503737 1105 40-41 0.5495097734427432 1105 42-43 0.5535943456328809 1105 44-45 0.6463735265995041 1105 46-47 0.7613468300399333 1105 48-49 0.6905105573078529 1105 50-51 0.6515625004891561 1105 52-53 0.7455453559661365 1105 54-55 0.6973463807657652 1105 56-57 0.6935769803407652 1105 58-59 0.6146444876677535 1105 60-61 0.6665460751469752 1105 62-63 0.665059288768127 1105 64-65 0.7293419223631403 1105 66-67 0.7166707315667864 1105 68-69 0.6894298073055793 1105 70-71 0.7505617580434034 1105 72-73 0.6836503400608436 1105 74-75 0.6060583366689656 1105 76-77 0.6188020317291958 1105 78-79 0.5089184864155669 1105 80-81 0.5995882421207028 1105 82-83 0.5615113479842719 1105 84-85 0.5556663551240533 1105 86-87 0.5640128152622594 1105 88-89 0.5633651632855319 1105 90-91 0.5652605763140315 1105 92-93 0.6416023155332127 1105 94-95 0.5341302085847062 1105 96-97 0.47359797884725907 1105 98-99 0.5945443796022509 1105 100-101 0.7142289636831656 1106 1 0.12289790250031274 1106 2 0.04884084205305328 1106 3 0.046216793206234286 1106 4 0.11710809221823837 1106 5 0.21541988137356327 1106 6 0.10794926833518304 1106 7 0.11720155094368323 1106 8 0.14172361298280833 1106 9 0.1486207482387627 1106 10-11 0.12318874667708712 1106 12-13 0.09756356457548065 1106 14-15 0.13170050226305108 1106 16-17 0.2295447258492942 1106 18-19 0.19097756277390232 1106 20-21 0.19987333231640037 1106 22-23 0.14536221754789125 1106 24-25 0.19192747241762476 1106 26-27 0.2471528027968617 1106 28-29 0.3459758988161781 1106 30-31 0.1590833909038878 1106 32-33 0.25474508136233 1106 34-35 0.3233468689963601 1106 36-37 0.36333427744259694 1106 38-39 0.4070791108815186 1106 40-41 0.3707874365614714 1106 42-43 0.22580003484358002 1106 44-45 0.3085346601416532 1106 46-47 0.26525911108139155 1106 48-49 0.2401898272706049 1106 50-51 0.17929389161466247 1106 52-53 0.3113499319862072 1106 54-55 0.30939435016549055 1106 56-57 0.23809358839443462 1106 58-59 0.32596274921547064 1106 60-61 0.406264430083759 1106 62-63 0.3935444694846666 1106 64-65 0.3442203863666151 1106 66-67 0.47009854009959895 1106 68-69 0.4808533474502994 1106 70-71 0.4380835000478527 1106 72-73 0.45128336996871354 1106 74-75 0.46435521910756705 1106 76-77 0.5621659880428709 1106 78-79 0.5528653231945739 1106 80-81 0.5374638098610518 1106 82-83 0.43622120420382515 1106 84-85 0.46047326609350847 1106 86-87 0.4384765124858063 1106 88-89 0.36775456099945814 1106 90-91 0.36392432913478956 1106 92-93 0.47158390257322935 1106 94-95 0.47641582824768847 1106 96-97 0.4573293397036622 1106 98-99 0.549707497773479 1106 100-101 0.45894361176816645 1107 1 0.14024748168632328 1107 2 0.14831205482106213 1107 3 0.20798303937188223 1107 4 0.19082158317790743 1107 5 0.279184602769206 1107 6 0.2136309769789264 1107 7 0.1875923869674807 1107 8 0.15518073059999438 1107 9 0.23166667452819212 1107 10-11 0.18232804030046879 1107 12-13 0.23736742674195455 1107 14-15 0.24355734163267329 1107 16-17 0.28834319957705645 1107 18-19 0.2766627307343654 1107 20-21 0.3029671257013007 1107 22-23 0.22998841312989526 1107 24-25 0.34337731837580066 1107 26-27 0.29456192479390353 1107 28-29 0.31407163050339904 1107 30-31 0.3947004157496181 1107 32-33 0.3827476243148169 1107 34-35 0.41799892808265326 1107 36-37 0.4417141947110892 1107 38-39 0.46957642459064175 1107 40-41 0.428483748612706 1107 42-43 0.4572708870748343 1107 44-45 0.5144959725454967 1107 46-47 0.531465008444421 1107 48-49 0.6332665827903838 1107 50-51 0.5279126895167039 1107 52-53 0.49901024878283096 1107 54-55 0.5052535397005755 1107 56-57 0.4821628840583543 1107 58-59 0.4825111231070949 1107 60-61 0.5432281264534069 1107 62-63 0.4497681725225604 1107 64-65 0.43350852241248816 1107 66-67 0.4649890583586611 1107 68-69 0.5805826527869584 1107 70-71 0.4810962853319438 1107 72-73 0.568518111523538 1107 74-75 0.5139794096211929 1107 76-77 0.5588469843761672 1107 78-79 0.5980016866222897 1107 80-81 0.5316659074004306 1107 82-83 0.5672502320022872 1107 84-85 0.4813750085626083 1107 86-87 0.43862558858952383 1107 88-89 0.534014580603646 1107 90-91 0.4586471185208154 1107 92-93 0.4621594552606254 1107 94-95 0.5224193159399917 1107 96-97 0.493970347337104 1107 98-99 0.487028455182152 1107 100-101 0.5554760744720149 1108 1 0.197767970577317 1108 2 0.22560925822561018 1108 3 0.09856959802775123 1108 4 0.24169877616036217 1108 5 0.2973178634636966 1108 6 0.33504403933779514 1108 7 0.26417346612802817 1108 8 0.286567816677028 1108 9 0.3556986829921911 1108 10-11 0.3369648855639369 1108 12-13 0.2800111647999657 1108 14-15 0.3585537096905256 1108 16-17 0.4160324158671074 1108 18-19 0.38083768770374604 1108 20-21 0.449634818786393 1108 22-23 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#Quality Count 5 1.0 6 24.0 7 419.0 8 724.0 9 714.0 10 1058.0 11 1877.0 12 3061.0 13 3880.0 14 4254.0 15 4823.0 16 5257.0 17 6052.0 18 7422.0 19 9138.0 20 11329.0 21 14296.0 22 17389.0 23 18276.0 24 17721.0 25 16029.0 26 15622.0 27 16445.0 28 18398.0 29 21090.0 30 25269.0 31 30689.0 32 38649.0 33 51779.0 34 75110.0 35 120207.0 36 228669.0 37 490295.0 38 719886.0 39 239782.0 40 489.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.713740254896535 18.862558097206637 13.9580425584818 23.465659089415027 2 14.953068324059096 20.25760658067557 40.78635209243856 24.002973002826767 3 17.847631816317797 25.80511000512941 30.89320220757087 25.45405597098192 4 12.023175827090013 17.520637281580665 39.50748684218176 30.948700049147565 5 12.99481289714385 38.16207784634387 35.571925157963136 13.27118409854914 6 30.22199583833269 38.37364939227404 18.16711334752158 13.237241421871696 7 26.509230485085123 33.312255184531445 22.585161907462155 17.593352422921278 8 25.72032039382449 35.428507286942626 20.386222045925024 18.464950273307863 9 26.191761365542053 15.788889967144026 20.35312905417099 37.66621961314293 10-11 24.21148121100673 26.23384312938063 29.346104843069902 20.20857081654274 12-13 25.252054560504945 24.048475866488563 28.881729672294416 21.81773990071208 14-15 22.424526736677723 25.370742128228187 26.744146006279617 25.460585128814472 16-17 21.857608011723865 28.60050632277384 27.09003485049794 22.451850815004363 18-19 21.8054641895817 27.702612960020534 29.131112197316515 21.36081065308125 20-21 22.75389144514859 26.6924046664696 29.188577730294803 21.365126158087012 22-23 22.40196805136396 26.718394648231165 28.86651233687988 22.013124963524998 24-25 22.16151190454895 26.759029032431176 29.042582687870063 22.036876375149813 26-27 22.175636134506018 26.99118071769755 28.897046361045437 21.936136786751 28-29 22.13916676318789 27.09940374478506 28.860509909338617 21.90091958268843 30-31 22.338708559412876 26.919158740373405 28.883876244732516 21.858256455481207 32-33 22.169688340042118 27.04656675862643 28.81990391405124 21.963840987280218 34-35 22.451604853579163 27.068278444432618 28.752108001214605 21.728008700773614 36-37 22.40907588714932 26.95075360344668 28.747703055690586 21.89246745371341 38-39 22.328780661886668 27.19906284224973 28.634158317766957 21.837998178096644 40-41 22.434544074722186 27.126727823111697 28.647999237966786 21.79072886419933 42-43 22.277084042335773 27.07232562788362 28.60571623296214 22.044874096818468 44-45 22.388996490801265 27.212970842838253 28.39734218555956 22.000690480800923 46-47 22.476871799985958 27.22547015526427 28.145477686155907 22.15218035859387 48-49 22.44064839009303 27.38722333252688 28.040295636689038 22.131832640691055 50-51 22.43065341217813 27.620014641412837 27.86944188669407 22.079890059714963 52-53 22.58207620958239 27.700444027452875 27.66853612256571 22.04894364039903 54-55 22.426561508468005 27.52391080454877 27.879861707070674 22.169665979912555 56-57 22.498181003460008 27.6053240362896 27.757372917321632 22.139122042928765 58-59 22.5998525125854 27.53558279218093 27.856137609603763 22.008427085629904 60-61 22.57574829291591 27.52659402009639 27.968228939105767 21.92942874788194 62-63 22.409031166890195 27.553851018034337 27.959061285984717 22.078056529090752 64-65 22.39091946194373 27.55729447798712 27.91007024211101 22.141715817958136 66-67 22.381595287915736 27.61370908487592 27.73662271708667 22.26807291012167 68-69 22.395257327079058 27.574489417621486 27.760704576626598 22.269548678672862 70-71 22.345036476079358 27.44303421591746 27.785211278628232 22.42671802937495 72-73 22.318696243453513 27.60199237698463 27.720277462375726 22.35903391718613 74-75 22.32480055882436 27.7131222209154 27.600896730636016 22.36118048962423 76-77 22.314403098577316 27.55948577068435 27.60009176597173 22.526019364766608 78-79 22.29653735505605 27.612948840470764 27.61234511697255 22.478168687500645 80-81 22.346847646574002 27.689219242411976 27.429305096365454 22.53462801464857 82-83 22.34284518338213 27.609594821036232 27.623592262143003 22.423967733438634 84-85 22.35905627731569 27.64210644942161 27.498017774514196 22.500819498748502 86-87 22.301903786151296 27.656797054544853 27.518477293064826 22.52282186623902 88-89 22.288286467247108 27.530417602251756 27.518477293064826 22.662818637436313 90-91 22.328892462534487 27.469754570745884 27.58663096797448 22.61472199874515 92-93 22.35525505528989 27.490169369037392 27.612814679693383 22.541760895979333 94-95 22.251705295281162 27.4541695604401 27.649977215027977 22.64414792925076 96-97 22.10334583562711 27.430959745953153 27.749748113140466 22.71594630527927 98-99 22.145919522316078 27.372063164682803 27.59716258899891 22.884854724002214 100-101 22.324017954289634 27.205211877879705 27.64601947209523 22.824750695735432 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 56.0 1 49.0 2 110.0 3 203.5 4 212.5 5 217.5 6 348.5 7 751.5 8 1393.5 9 1867.5 10 1844.5 11 1700.5 12 1747.5 13 1936.0 14 2098.5 15 2287.0 16 2570.0 17 2797.0 18 3042.0 19 3354.0 20 3844.0 21 4606.5 22 5653.0 23 7102.0 24 8932.0 25 11210.0 26 13936.0 27 17329.5 28 21230.5 29 24911.0 30 28860.0 31 32857.5 32 36660.5 33 41387.5 34 46059.5 35 49540.5 36 53473.5 37 57488.5 38 61486.0 39 65138.5 40 68093.5 41 71333.5 42 73871.0 43 77005.5 44 80467.0 45 82991.5 46 84951.0 47 86604.5 48 88570.0 49 88412.0 50 85405.5 51 82379.5 52 79489.5 53 76324.0 54 72507.5 55 66735.5 56 59858.5 57 53121.0 58 47312.5 59 41146.0 60 34326.5 61 28090.0 62 22075.0 63 16543.0 64 12074.0 65 8890.5 66 6680.0 67 5118.0 68 3854.5 69 2909.0 70 2245.5 71 1694.0 72 1201.0 73 888.5 74 696.0 75 513.0 76 379.5 77 271.0 78 188.0 79 134.5 80 106.5 81 76.5 82 61.5 83 50.0 84 38.0 85 25.0 86 17.5 87 18.5 88 20.0 89 21.5 90 22.5 91 17.0 92 12.0 93 9.0 94 4.5 95 1.5 96 0.5 97 2.5 98 2.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.1180064781767371E-4 24-25 1.3416077738120845E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2236123.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.11773207601418 #Duplication Level Percentage of deduplicated Percentage of total 1 75.25811864197347 48.25379887630943 2 14.903699549714581 19.11182829340025 3 4.791978177277468 9.217523186543504 4 2.0389336435774106 5.229272043186711 5 1.0041200729709685 3.2190950905450184 6 0.5703063360582836 2.1940049313982994 7 0.3672925946292692 1.6484977723160494 8 0.24092169648853562 1.2357882229400585 9 0.1633615148841176 0.9426932858584489 >10 0.637963375685125 6.670166348357482 >50 0.017338119187074504 0.7350287539940527 >100 0.005405512533573468 0.6620353649408827 >500 3.5047813761521684E-4 0.16943784947884064 >1k 7.009562752297327E-5 0.15797719918241568 >5k 1.4019125504594653E-4 0.5528527815484766 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6714 0.3002518197791445 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5587 0.24985208774293724 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3515 0.15719171083164923 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.4720259127069485E-5 0.0 12-13 0.0 0.0 0.0 8.944051825413897E-5 0.0 14-15 0.0 0.0 0.0 8.944051825413897E-5 0.0 16-17 0.0 0.0 0.0 1.3416077738120845E-4 0.0 18-19 0.0 0.0 0.0 1.7888103650827794E-4 0.0 20-21 0.0 4.4720259127069485E-5 0.0 1.7888103650827794E-4 0.0 22-23 0.0 4.4720259127069485E-5 0.0 2.2360129563534742E-4 2.2360129563534742E-5 24-25 0.0 4.4720259127069485E-5 0.0 4.4720259127069485E-4 4.4720259127069485E-5 26-27 0.0 4.4720259127069485E-5 0.0 5.590032390883685E-4 4.4720259127069485E-5 28-29 0.0 4.4720259127069485E-5 0.0 0.0013416077738120846 4.4720259127069485E-5 30-31 0.0 4.4720259127069485E-5 0.0 0.0031304181388948638 4.4720259127069485E-5 32-33 0.0 4.4720259127069485E-5 0.0 0.0059701545934637765 4.4720259127069485E-5 34-35 0.0 4.4720259127069485E-5 0.0 0.01046454063573426 4.4720259127069485E-5 36-37 0.0 4.4720259127069485E-5 0.0 0.01777630300301012 4.4720259127069485E-5 38-39 0.0 4.4720259127069485E-5 0.0 0.028911647525650425 4.4720259127069485E-5 40-41 0.0 4.4720259127069485E-5 0.0 0.04322213044631266 4.4720259127069485E-5 42-43 0.0 4.4720259127069485E-5 0.0 0.05820341725388094 4.4720259127069485E-5 44-45 0.0 4.4720259127069485E-5 0.0 0.0760691607751452 4.4720259127069485E-5 46-47 0.0 4.4720259127069485E-5 0.0 0.0988541328003871 4.4720259127069485E-5 48-49 0.0 4.4720259127069485E-5 0.0 0.130001793282391 4.4720259127069485E-5 50-51 0.0 4.4720259127069485E-5 0.0 0.16244634127907992 4.4720259127069485E-5 52-53 0.0 4.4720259127069485E-5 0.0 0.19779770611902833 4.4720259127069485E-5 54-55 2.2360129563534742E-5 4.4720259127069485E-5 0.0 0.23768817726037433 4.4720259127069485E-5 56-57 4.4720259127069485E-5 4.4720259127069485E-5 0.0 0.2858966165993552 4.4720259127069485E-5 58-59 4.4720259127069485E-5 4.4720259127069485E-5 0.0 0.3379062779641371 4.4720259127069485E-5 60-61 4.4720259127069485E-5 4.4720259127069485E-5 0.0 0.399038872190841 4.4720259127069485E-5 62-63 4.4720259127069485E-5 4.4720259127069485E-5 0.0 0.46960744109335667 4.4720259127069485E-5 64-65 4.4720259127069485E-5 4.4720259127069485E-5 0.0 0.5442679137059991 4.4720259127069485E-5 66-67 4.4720259127069485E-5 4.4720259127069485E-5 0.0 0.6373084128198672 4.4720259127069485E-5 68-69 4.4720259127069485E-5 4.4720259127069485E-5 0.0 0.7501376265974635 4.4720259127069485E-5 70-71 4.4720259127069485E-5 4.4720259127069485E-5 0.0 0.8755779534488934 4.4720259127069485E-5 72-73 4.4720259127069485E-5 4.4720259127069485E-5 0.0 1.0063847113955717 4.4720259127069485E-5 74-75 4.4720259127069485E-5 4.4720259127069485E-5 0.0 1.134061051203355 4.4720259127069485E-5 76-77 4.4720259127069485E-5 4.4720259127069485E-5 0.0 1.2698541180427014 4.4720259127069485E-5 78-79 4.4720259127069485E-5 4.4720259127069485E-5 0.0 1.4302209672723727 4.4720259127069485E-5 80-81 4.4720259127069485E-5 4.4720259127069485E-5 0.0 1.6111814958300594 4.4720259127069485E-5 82-83 4.4720259127069485E-5 4.4720259127069485E-5 0.0 1.8089121215603972 4.4720259127069485E-5 84-85 4.4720259127069485E-5 4.4720259127069485E-5 0.0 2.0067992681976796 4.4720259127069485E-5 86-87 4.4720259127069485E-5 4.4720259127069485E-5 0.0 2.2259061777907565 4.4720259127069485E-5 88-89 4.4720259127069485E-5 4.4720259127069485E-5 0.0 2.4846575970999805 4.4720259127069485E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4470 0.0 43.887024 1 GTATCAA 7105 0.0 36.101337 1 TCAACGC 8355 0.0 29.961102 4 CAACGCA 8470 0.0 29.61039 5 ATCAACG 8600 0.0 29.218023 3 AACGCAG 8935 0.0 28.70733 6 TATCAAC 8860 0.0 28.41422 2 ACGCAGA 9770 0.0 26.156603 7 CGCAGAG 9875 0.0 25.782278 8 GTACATG 6160 0.0 24.598215 1 GTATAGG 780 0.0 24.358973 1 TACATGG 6295 0.0 23.542494 2 GAGTACT 6575 0.0 22.90114 12-13 ACATGGG 6330 0.0 22.586885 3 GCAGAGT 11110 0.0 22.574257 9 AGAGTAC 8995 0.0 22.020567 10-11 GTACTTT 7105 0.0 20.99226 14-15 CAGAGTA 10800 0.0 20.121527 10-11 AGTACTT 7090 0.0 19.596264 12-13 GTATAGC 945 0.0 19.100529 1 >>END_MODULE