FastQCFastQC Report
Fri 10 Feb 2017
SRR2031802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2236123
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86280.38584639574835555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75610.3381298792597724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44260.19793186689640954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45650.048.2508541
GTATCAA77150.037.7997861
TCAACGC92750.031.08564
CAACGCA93500.030.836255
ATCAACG94450.030.5763823
TATCAAC96550.030.3541052
AACGCAG96400.030.154976
ACGCAGA106550.027.1932357
CGCAGAG108400.026.5976898
GCAGAGT121850.023.1550339
GAGTACT79950.022.90289912-13
GTACATG53150.022.3769841
AGAGTAC101450.021.72453310-11
TACATGG52600.021.4016422
GTACTTT85550.021.20937314-15
CAGAGTA118500.020.82340210-11
ACATGGG51850.020.6119123
GTATAGC8850.019.8894731
AGTACTT84700.019.76788512-13
GGACCGT2803.493551E-818.6603246
Produced by FastQC (version 0.11.5)