FastQCFastQC Report
Fri 10 Feb 2017
SRR2031799_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031799_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642225
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23410.36451399431663356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.3621783642804313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.18482619019813928No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.056.6806761
TAGCCCG500.001615285238.05
GTATCAA19200.036.3671881
TATCAAC24050.028.8357582
ATCAACG23900.028.8179913
TCAACGC24100.028.5788384
CAACGCA24550.027.8615065
AACGCAG25200.027.331356
TCAACCG908.943232E-426.3888875
ACGCAGA27850.024.3895877
CGCAGAG27750.023.9639638
GAGTACT21350.022.24824512-13
GTACATG15300.021.7320251
GCAGAGT31200.021.0096179
CAGAGTA30800.020.74269510-11
GTACTTT23300.020.28433614-15
TACATGG15950.020.2507842
AGAGTAC27500.019.9510-11
GTATAGC1951.03148395E-519.487181
GTAGGAC2201.3428598E-619.4318183
Produced by FastQC (version 0.11.5)