##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031799_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 642225 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.413028144341936 34.0 31.0 34.0 31.0 34.0 2 32.54707929463973 34.0 31.0 34.0 31.0 34.0 3 32.61449336291798 34.0 31.0 34.0 31.0 34.0 4 35.82950679279069 37.0 37.0 37.0 35.0 37.0 5 35.85461948693994 37.0 37.0 37.0 35.0 37.0 6 35.927997197243954 37.0 37.0 37.0 35.0 37.0 7 35.94496009965355 37.0 37.0 37.0 35.0 37.0 8 35.94457082798085 37.0 37.0 37.0 35.0 37.0 9 37.755526489937324 39.0 39.0 39.0 35.0 39.0 10-11 37.78471096578302 39.0 39.0 39.0 35.0 39.0 12-13 37.75818521546187 39.0 39.0 39.0 35.0 39.0 14-15 39.299371715520266 41.0 40.0 41.0 36.5 41.0 16-17 39.23156292576589 41.0 40.0 41.0 36.0 41.0 18-19 39.229843123515906 41.0 40.0 41.0 36.0 41.0 20-21 39.17379968079723 41.0 40.0 41.0 36.0 41.0 22-23 39.08054731597181 41.0 39.0 41.0 36.0 41.0 24-25 39.04055510140527 41.0 39.0 41.0 36.0 41.0 26-27 38.913281949472534 41.0 39.0 41.0 35.0 41.0 28-29 38.79302347308186 41.0 39.0 41.0 35.0 41.0 30-31 38.630401339094554 40.5 38.5 41.0 35.0 41.0 32-33 38.50073260928802 40.0 38.0 41.0 35.0 41.0 34-35 38.30798318346374 40.0 38.0 41.0 34.0 41.0 36-37 38.19290357740667 40.0 38.0 41.0 34.0 41.0 38-39 38.00190120284947 40.0 38.0 41.0 33.0 41.0 40-41 37.904499201993076 40.0 38.0 41.0 33.0 41.0 42-43 37.73973685234925 40.0 38.0 41.0 33.0 41.0 44-45 37.892885670909735 40.0 38.0 41.0 33.0 41.0 46-47 37.757577951652465 40.0 38.0 41.0 33.0 41.0 48-49 37.58362956907626 40.0 38.0 41.0 32.0 41.0 50-51 36.519588150570286 39.0 36.5 40.0 30.5 40.5 52-53 36.547758184436915 39.5 36.5 40.0 30.5 40.5 54-55 37.33997975787302 40.0 37.0 41.0 31.5 41.0 56-57 37.34267351784811 40.0 37.0 41.0 31.5 41.0 58-59 37.22297637120947 40.0 37.0 41.0 31.0 41.0 60-61 37.03087080073183 40.0 36.0 41.0 31.0 41.0 62-63 36.76645490287672 39.5 35.5 41.0 31.0 41.0 64-65 36.45643505002141 39.0 35.0 41.0 30.0 41.0 66-67 36.18519521974386 39.0 35.0 41.0 30.0 41.0 68-69 35.875094398380625 37.5 35.0 40.5 30.0 41.0 70-71 35.525972206002564 37.0 35.0 40.0 29.0 41.0 72-73 35.13783798512982 37.0 35.0 39.0 29.0 41.0 74-75 34.78717583401456 36.0 35.0 39.0 29.0 41.0 76-77 34.41497294561874 36.0 35.0 38.5 29.0 40.5 78-79 34.0375545953521 35.0 34.0 37.0 28.0 39.0 80-81 33.69230487757406 35.0 34.0 37.0 27.5 39.0 82-83 33.41121647397719 35.0 34.0 36.5 27.0 39.0 84-85 33.116362645490284 35.0 34.0 36.0 26.5 37.0 86-87 32.86602436840671 35.0 34.0 36.0 26.0 37.0 88-89 32.67142123087703 35.0 34.0 35.0 26.0 36.5 90-91 32.5074389816653 35.0 34.0 35.0 26.0 36.0 92-93 32.384850325041846 35.0 34.0 35.0 25.0 36.0 94-95 32.223910623223944 35.0 34.0 35.0 25.0 36.0 96-97 32.12819183308031 35.0 33.5 35.0 25.0 36.0 98-99 32.01745104908716 35.0 33.0 35.0 24.5 35.0 100-101 30.500337109268557 34.0 30.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1046345533534776 1101 2 0.11754515671674426 1101 3 0.20740330486941616 1101 4 0.18720184331223066 1101 5 0.18649927555065915 1101 6 0.08194260101386419 1101 7 0.1589417524679746 1101 8 0.2777241962046162 1101 9 0.33076981257323723 1101 10-11 0.3256686594157756 1101 12-13 0.29181428251907704 1101 14-15 0.26426399719571947 1101 16-17 0.36407142900554845 1101 18-19 0.3761375525922759 1101 20-21 0.3597957823396385 1101 22-23 0.46761731571876197 1101 24-25 0.36080859751246663 1101 26-27 0.44616963010009414 1101 28-29 0.5096973039352477 1101 30-31 0.425581492118404 1101 32-33 0.2710288455894698 1101 34-35 0.4216436583197236 1101 36-37 0.2546101093572375 1101 38-39 0.4139381591433775 1101 40-41 0.25302983086428554 1101 42-43 0.2990810326408493 1101 44-45 0.49734639509811274 1101 46-47 0.4514164114403414 1101 48-49 0.38394187864814455 1101 50-51 0.5249547149831457 1101 52-53 0.31392239129777266 1101 54-55 0.12404183922350853 1101 56-57 0.2321438047273361 1101 58-59 0.2092596250206853 1101 60-61 0.18780481652946435 1101 62-63 0.16121246844964077 1101 64-65 0.15432169540569873 1101 66-67 0.19995846252962934 1101 68-69 0.07790685233528194 1101 70-71 -0.004734799798221445 1101 72-73 -0.09908207700069482 1101 74-75 -0.0711277559836816 1101 76-77 -0.08017986787534426 1101 78-79 -0.21177669619641648 1101 80-81 -0.06334173501718254 1101 82-83 -0.06457896601644109 1101 84-85 0.07858532604634405 1101 86-87 -0.28752463567072795 1101 88-89 0.026936083504509156 1101 90-91 0.21684211537972686 1101 92-93 0.08294801503310367 1101 94-95 -0.08699077213984197 1101 96-97 0.03265267771974578 1101 98-99 0.07987273160003383 1101 100-101 0.14096073037425327 1102 1 5.481058474146039E-4 1102 2 -0.04803090032140034 1102 3 0.12255832723803906 1102 4 0.13965274733116217 1102 5 0.08403761381769925 1102 6 0.05751888062540189 1102 7 0.060857367475357194 1102 8 0.1625387554722053 1102 9 0.15845720082284487 1102 10-11 0.20121118206261457 1102 12-13 0.3034678544510925 1102 14-15 0.22024825383768132 1102 16-17 0.27081688665354164 1102 18-19 0.2159737385746041 1102 20-21 0.30363124124888685 1102 22-23 0.4292957416797165 1102 24-25 0.32711469528153003 1102 26-27 0.45264095151832606 1102 28-29 0.2889564312991624 1102 30-31 0.21619742688955768 1102 32-33 0.22819088643910845 1102 34-35 0.39844409159593397 1102 36-37 0.3234038357551867 1102 38-39 0.2686218452555451 1102 40-41 0.2613114158836112 1102 42-43 0.1362741595744481 1102 44-45 0.1762898391988017 1102 46-47 0.5371127323021412 1102 48-49 0.41486474212904767 1102 50-51 0.3909247269279206 1102 52-53 0.43240748258995154 1102 54-55 0.5275340157687225 1102 56-57 0.5053730479036105 1102 58-59 0.41695955157145903 1102 60-61 0.338156853446975 1102 62-63 0.1367868932828955 1102 64-65 0.23548679292994024 1102 66-67 0.18774289277883582 1102 68-69 0.41546351581173013 1102 70-71 0.30452351146325185 1102 72-73 0.38007021513429606 1102 74-75 0.32276037862871476 1102 76-77 0.19011511606227316 1102 78-79 0.1789323484443841 1102 80-81 0.12030913635765472 1102 82-83 0.11659206679829026 1102 84-85 0.17222566228054603 1102 86-87 0.05978074244437437 1102 88-89 0.1872686823456462 1102 90-91 0.1659469993818945 1102 92-93 -0.05509200366306999 1102 94-95 -0.04958731334930633 1102 96-97 -0.022360046059255012 1102 98-99 -0.04967425735290476 1102 100-101 -0.056230022443156 1103 1 0.2952128594369796 1103 2 0.2976662025879051 1103 3 0.2589566543131667 1103 4 0.29638260659788784 1103 5 0.34366659897099794 1103 6 0.29779322402274033 1103 7 0.27502024360362043 1103 8 0.5074319589605167 1103 9 0.4617613292025311 1103 10-11 0.4301088222616656 1103 12-13 0.31849263286994756 1103 14-15 0.41594688313828954 1103 16-17 0.5298454912266095 1103 18-19 0.4464537377605282 1103 20-21 0.5921904296157479 1103 22-23 0.5641982052752823 1103 24-25 0.4176704034762224 1103 26-27 0.5569596213191872 1103 28-29 0.5096827900418219 1103 30-31 0.33306812119408136 1103 32-33 0.4869701804322517 1103 34-35 0.30134525267091306 1103 36-37 0.34067749736441044 1103 38-39 0.42660334539291966 1103 40-41 0.5092889474035971 1103 42-43 0.562386644437737 1103 44-45 0.6226575366884006 1103 46-47 0.5830265827768528 1103 48-49 0.5353580717990383 1103 50-51 0.21650908039944028 1103 52-53 0.32003455466615094 1103 54-55 0.4586886799117522 1103 56-57 0.4772925358502036 1103 58-59 0.505337608170052 1103 60-61 0.5191207186767883 1103 62-63 0.4681613577739441 1103 64-65 0.4501075864498958 1103 66-67 0.43270325748459726 1103 68-69 0.5023457089307612 1103 70-71 0.38883119124670174 1103 72-73 0.5011690133555433 1103 74-75 0.324622576021639 1103 76-77 0.42450267699072697 1103 78-79 0.47849681812516565 1103 80-81 0.4001068386299451 1103 82-83 0.4067234382100011 1103 84-85 0.45708589571665215 1103 86-87 0.5439164488073871 1103 88-89 0.6293370443242523 1103 90-91 0.4177506851081034 1103 92-93 0.4120142636996391 1103 94-95 0.24158200415025988 1103 96-97 0.33510044584578225 1103 98-99 0.37744564577212714 1103 100-101 0.5837451482582736 1104 1 0.045893572260666815 1104 2 0.08376276052830178 1104 3 0.20986082872587986 1104 4 0.11244930706546086 1104 5 0.056565402794255704 1104 6 0.17549964431830745 1104 7 0.13544125274864172 1104 8 0.2188942239972249 1104 9 0.28719839378105405 1104 10-11 0.2007696301034727 1104 12-13 0.20176212003499217 1104 14-15 0.309628929220203 1104 16-17 0.26656454930784435 1104 18-19 0.2943684092344441 1104 20-21 0.39782579288166886 1104 22-23 0.3270284683590532 1104 24-25 0.23636136924340434 1104 26-27 0.21705858288003554 1104 28-29 0.2643351100989051 1104 30-31 0.09911416554839292 1104 32-33 0.17344324298271374 1104 34-35 0.1855536540755338 1104 36-37 0.2704607959579164 1104 38-39 0.3150039988772235 1104 40-41 0.4172800464966002 1104 42-43 0.3925182802141265 1104 44-45 0.2900518618907313 1104 46-47 0.22100472485335843 1104 48-49 0.33121464325979844 1104 50-51 0.3925085546074669 1104 52-53 0.19856252492134985 1104 54-55 0.5337877353640295 1104 56-57 0.43503670049820187 1104 58-59 0.37949962721123853 1104 60-61 0.6288516258557308 1104 62-63 0.5278387468412262 1104 64-65 0.404903560385641 1104 66-67 0.5282706433595692 1104 68-69 0.5930729919007334 1104 70-71 0.33084384781065523 1104 72-73 0.43795191760506924 1104 74-75 0.3907737787707788 1104 76-77 0.4550126952056601 1104 78-79 0.4016185201888973 1104 80-81 0.31710212528046355 1104 82-83 0.15513667314512247 1104 84-85 0.21446106706850543 1104 86-87 0.4104497896520414 1104 88-89 0.16060758307324363 1104 90-91 0.359505140256168 1104 92-93 0.27378261477844745 1104 94-95 0.23575604129370475 1104 96-97 0.18540934863060698 1104 98-99 0.0035183552908435445 1104 100-101 0.011293830961594864 1105 1 -0.05871545377303988 1105 2 -0.06827714182333722 1105 3 -0.07674120737123502 1105 4 0.015608828658749019 1105 5 -0.04844171008092246 1105 6 0.11480603653922117 1105 7 0.1379117328530839 1105 8 0.12865437667524304 1105 9 0.2813558839606003 1105 10-11 0.09746902185019479 1105 12-13 0.10889519227018951 1105 14-15 0.28085691004178415 1105 16-17 0.5087974652354106 1105 18-19 0.44874999888886435 1105 20-21 0.3906485212986226 1105 22-23 0.5044054273268372 1105 24-25 0.4761428312758724 1105 26-27 0.35845405288594634 1105 28-29 0.4353134085118242 1105 30-31 0.5532361206231187 1105 32-33 0.4908955457394697 1105 34-35 0.4874044651774767 1105 36-37 0.4959140588064628 1105 38-39 0.5875588382667516 1105 40-41 0.21460261977677675 1105 42-43 0.49797031367683786 1105 44-45 0.46998565068224707 1105 46-47 0.3966151734621022 1105 48-49 0.4267247505676224 1105 50-51 0.4522947585567252 1105 52-53 0.37723472967848437 1105 54-55 0.49420004795586436 1105 56-57 0.4466265890924248 1105 58-59 0.4709030004117949 1105 60-61 0.3765523816353067 1105 62-63 0.5529182178540637 1105 64-65 0.37820978868844435 1105 66-67 0.33186015728382756 1105 68-69 0.32104503215433766 1105 70-71 0.5061484135154757 1105 72-73 0.5329892967886352 1105 74-75 0.3112778855048788 1105 76-77 0.25810752768720846 1105 78-79 0.3243106409965506 1105 80-81 0.5167382856157943 1105 82-83 0.3745571640798673 1105 84-85 0.3202116656623204 1105 86-87 0.40255830344165133 1105 88-89 0.36440901969839956 1105 90-91 0.3635635770628127 1105 92-93 0.1655308014343433 1105 94-95 0.4239501320457961 1105 96-97 0.526517083809189 1105 98-99 0.4451357001686844 1105 100-101 0.27501904566505075 1106 1 0.2719486579997863 1106 2 0.26289719283185065 1106 3 0.26128428676545923 1106 4 0.3347248958607736 1106 5 0.21691670732107582 1106 6 0.23642616426501917 1106 7 0.11698976010465145 1106 8 0.09129188882846506 1106 9 0.2761629488467747 1106 10-11 0.16495875361469103 1106 12-13 0.21748762906633345 1106 14-15 0.20833297917130977 1106 16-17 0.2829118742130703 1106 18-19 0.29946586993853685 1106 20-21 0.3409046248398582 1106 22-23 0.3053263796295056 1106 24-25 0.2610810517885369 1106 26-27 0.2481679595775219 1106 28-29 0.39259499768603945 1106 30-31 0.48607661644638256 1106 32-33 0.48310897764029903 1106 34-35 0.4747523549806658 1106 36-37 0.3243643701841492 1106 38-39 0.14755112823208094 1106 40-41 0.14774216290053488 1106 42-43 -0.056198349000645464 1106 44-45 0.22309730162176322 1106 46-47 0.12436024343350027 1106 48-49 0.1444340199001104 1106 50-51 0.01873002969767157 1106 52-53 0.21494558637105143 1106 54-55 0.3440967278221905 1106 56-57 0.29868462977986354 1106 58-59 0.2651641268138434 1106 60-61 0.3631632828306479 1106 62-63 0.3726678118016835 1106 64-65 0.3928517681264623 1106 66-67 0.29796576775132877 1106 68-69 0.362314976908543 1106 70-71 0.3142620911736671 1106 72-73 0.43926702244178983 1106 74-75 0.41752329704215185 1106 76-77 0.3619733163925787 1106 78-79 0.38740395225801905 1106 80-81 0.41994475297428124 1106 82-83 0.3037878330104107 1106 84-85 0.2663533885716163 1106 86-87 0.36269147161394955 1106 88-89 0.30935880526305937 1106 90-91 0.5050172020546597 1106 92-93 0.3653525068826724 1106 94-95 0.4160983688005544 1106 96-97 0.3647353288125501 1106 98-99 0.3096275890421438 1106 100-101 0.4884410254999061 1107 1 0.08845322189696958 1107 2 0.022606300155608494 1107 3 0.15931175937944886 1107 4 0.22855064369429812 1107 5 0.20286284336143012 1107 6 0.15417103742594662 1107 7 0.255759528681736 1107 8 0.19666043861667504 1107 9 0.1003413686203416 1107 10-11 0.17012364703627014 1107 12-13 0.16290719792370112 1107 14-15 0.2414153287779115 1107 16-17 0.16606534608172296 1107 18-19 0.00267870658827718 1107 20-21 0.08644439294454287 1107 22-23 0.18152509155547847 1107 24-25 0.15957567431566133 1107 26-27 0.14701294453949032 1107 28-29 0.2270927065519075 1107 30-31 0.20735345788310866 1107 32-33 0.36032465918053447 1107 34-35 0.14413225283755082 1107 36-37 0.07621328655381632 1107 38-39 0.10379387822289488 1107 40-41 0.2461362694632001 1107 42-43 0.1252683967490853 1107 44-45 0.29819033793873473 1107 46-47 0.41842848931182175 1107 48-49 0.2920507680118334 1107 50-51 0.1448473840544935 1107 52-53 0.2264143791821951 1107 54-55 0.29597241345653913 1107 56-57 0.08228268139730943 1107 58-59 0.21104428449006463 1107 60-61 0.2543462218859531 1107 62-63 0.24393520526263757 1107 64-65 0.1366699050906206 1107 66-67 0.3327710607173131 1107 68-69 0.2983154388095741 1107 70-71 0.19156831581504008 1107 72-73 0.19912572271525875 1107 74-75 0.133507959828151 1107 76-77 0.15348798437496214 1107 78-79 0.2537492127859551 1107 80-81 0.35375643382166544 1107 82-83 0.3076890596725974 1107 84-85 0.3563952221087803 1107 86-87 0.37499877227478606 1107 88-89 0.43279529649481674 1107 90-91 0.4351769778227066 1107 92-93 0.5208556903256252 1107 94-95 0.5041902451160567 1107 96-97 0.42688332610412516 1107 98-99 0.4880381770736619 1107 100-101 0.36064665544369134 1108 1 0.3159389540712212 1108 2 0.23275150688857593 1108 3 0.1933161420702305 1108 4 0.18307179860556033 1108 5 0.28140210697306145 1108 6 0.22411589258042142 1108 7 0.30639164754416015 1108 8 0.269010957556155 1108 9 0.32953201566925117 1108 10-11 0.22136982422220086 1108 12-13 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-0.5901170705372749 2316 64-65 -0.394088094008076 2316 66-67 -0.17089185050395628 2316 68-69 -0.40015233946892437 2316 70-71 -0.4124001384436369 2316 72-73 -0.3178026233865907 2316 74-75 -0.6591869477878873 2316 76-77 -0.9730825428895784 2316 78-79 -0.3834693541360892 2316 80-81 -0.11646979420898873 2316 82-83 -0.13175483397319 2316 84-85 -0.18356151516026387 2316 86-87 0.11484645140952665 2316 88-89 -0.11653232856151163 2316 90-91 0.04682276421296905 2316 92-93 -0.30755170039092405 2316 94-95 -0.28682913298218793 2316 96-97 -0.25768539854661654 2316 98-99 -0.35289107033508316 2316 100-101 -0.007937391424267304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 11.0 7 179.0 8 423.0 9 383.0 10 411.0 11 576.0 12 759.0 13 909.0 14 984.0 15 1059.0 16 1305.0 17 1496.0 18 1938.0 19 2418.0 20 3098.0 21 3994.0 22 4826.0 23 5099.0 24 4938.0 25 4376.0 26 4338.0 27 4463.0 28 4971.0 29 5550.0 30 6661.0 31 8144.0 32 10380.0 33 13849.0 34 20093.0 35 33850.0 36 63922.0 37 138856.0 38 214462.0 39 73396.0 40 108.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.86161392035502 19.585036396901398 14.12137490754798 23.43197477519561 2 15.403168671415782 21.120946708708008 39.8972322784071 23.578652341469112 3 17.596325275409708 25.749386897115496 31.580832262836232 25.073455564638564 4 12.530343726886993 18.17649577640235 38.57900268597454 30.714157810736115 5 13.058974658414108 37.963642025769786 35.71583167892872 13.261551636887384 6 30.2882946008019 37.94744832418545 18.067032582039005 13.697224492973648 7 25.93545875666628 33.533107555763166 23.06029818210129 17.471135505469267 8 24.992954182724123 35.17583401455876 21.252520534080734 18.578691268636383 9 25.72961189614232 15.55825450581961 21.15504690723656 37.55708669080151 10-11 23.563626454902877 26.38857098368952 30.05185098680369 19.995951574603914 12-13 24.60516174238001 24.853672778231928 29.262875160574563 21.2782903188135 14-15 21.94145354042586 26.440344116158666 26.99980536416365 24.61839697925182 16-17 21.26489937327261 29.26848067266145 27.740589357312466 21.726030596753475 18-19 21.20643076803301 28.117793608159136 29.80972400638405 20.8660516174238 20-21 22.03137529681965 27.331231266300755 29.86710265094009 20.77029078593951 22-23 21.849585425668575 27.429172019152166 29.471836194480126 21.249406360699133 24-25 21.72216149555761 27.481307086957468 29.59451716073258 21.20201425675234 26-27 21.600685118143954 27.592588267351786 29.569543384327922 21.237183230176342 28-29 21.520728716571295 27.635096734010666 29.685468488458095 21.158706060959943 30-31 21.6712989995718 27.41266690022967 29.678461598349486 21.23757250184904 32-33 21.50352290863794 27.649966911907818 29.506714936354083 21.33979524310016 34-35 21.80341780528631 27.68866051617424 29.40231227373584 21.105609404803612 36-37 21.725174199073532 27.58433570789054 29.40480361244112 21.285686480594805 38-39 21.68873837050878 27.741757172330573 29.337226050060337 21.232278407100317 40-41 21.717622328623147 27.73077971116042 29.404881466775663 21.146716493440774 42-43 21.691618980886762 27.857993693798903 29.07306629296586 21.377321032348476 44-45 21.76449063801627 27.91856436607108 28.935964809840787 21.38098018607186 46-47 21.850597532017595 28.057923624897818 28.678111253844058 21.413367589240533 48-49 21.88656623457511 28.017672933940595 28.626960956051228 21.468799875433064 50-51 21.744092802366772 28.224843318151738 28.541165479388063 21.489898400093423 52-53 21.78278640663319 28.30308692436451 28.437074234107985 21.47705243489431 54-55 21.747674101755614 28.273113005566586 28.43956557281327 21.53964731986453 56-57 21.839464362178365 28.362567635953134 28.34551753668886 21.45245046517965 58-59 21.67900657869127 28.31772353925805 28.5538557359181 21.449414146132586 60-61 21.7117053991981 28.305033282728015 28.51866557670598 21.4645957413679 62-63 21.619525867102652 28.333527969169687 28.539530538362722 21.507415625364942 64-65 21.66110008174705 28.16536260656312 28.56421036241193 21.6093269492779 66-67 21.510451944412004 28.324341157693954 28.465802483553272 21.699404414340766 68-69 21.58760558994122 28.282922651718636 28.426408190275993 21.703063568064152 70-71 21.604967106543658 28.14169488886294 28.439954844485964 21.81338316010744 72-73 21.469344855774843 28.2043676281677 28.50402896181245 21.822258554245007 74-75 21.4630386546771 28.372455136439722 28.315699326560008 21.848806882323174 76-77 21.430028416832105 28.252715169917085 28.37891704620655 21.93833936704426 78-79 21.401845147728597 28.24570827980848 28.42500681225427 21.92743976020865 80-81 21.35077270427031 28.385846081980613 28.287516057456497 21.975865156292578 82-83 21.404725758106583 28.23838997236171 28.393320098096464 21.963564171435245 84-85 21.270504885359493 28.169255323290127 28.409046673673554 22.151193117676826 86-87 21.3175289034217 28.019230020631397 28.382420491260852 22.280820584686055 88-89 21.316438942738138 28.03900502160458 28.435828564755344 22.20872747090194 90-91 21.322745143835885 27.98123710537584 28.48456537817743 22.211452372610847 92-93 21.355599673011795 27.86266495387131 28.55401144458718 22.227723928529723 94-95 21.190704192455915 27.870761804663474 28.562419712717507 22.376114290163105 96-97 21.19716610222274 27.766670559383392 28.655222079489274 22.380941258904592 98-99 21.20713145704387 27.841877846549107 28.62820662540387 22.322784071003156 100-101 21.27891315348982 27.612051850986802 28.661917552259723 22.447117443263654 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 545.0 1 339.0 2 177.0 3 231.0 4 183.0 5 158.0 6 247.5 7 505.0 8 884.5 9 1110.0 10 927.5 11 690.0 12 726.5 13 770.0 14 796.5 15 858.0 16 897.5 17 959.0 18 1033.5 19 1106.0 20 1276.5 21 1523.5 22 1866.5 23 2377.0 24 2942.0 25 3654.0 26 4635.0 27 5620.5 28 6813.0 29 8209.5 30 9542.5 31 10855.5 32 12226.5 33 13682.0 34 14937.5 35 15952.0 36 17073.0 37 18198.0 38 19063.5 39 19832.5 40 20729.0 41 21767.5 42 22363.5 43 22689.0 44 23124.5 45 23961.5 46 24464.0 47 24435.5 48 24912.0 49 24504.5 50 23179.0 51 22171.5 52 21277.0 53 20340.0 54 18973.0 55 17129.5 56 15214.0 57 13115.0 58 11180.5 59 9537.5 60 7879.5 61 6384.0 62 5081.5 63 3830.0 64 2723.0 65 1956.5 66 1433.0 67 1046.0 68 786.0 69 626.5 70 507.5 71 390.0 72 328.5 73 271.5 74 192.0 75 150.0 76 109.0 77 74.0 78 54.5 79 37.0 80 26.0 81 22.0 82 20.0 83 16.0 84 11.5 85 9.5 86 8.0 87 6.5 88 6.5 89 4.5 90 1.5 91 3.0 92 2.5 93 1.5 94 2.0 95 0.5 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.671260072404531E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 642225.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.9228999284621 #Duplication Level Percentage of deduplicated Percentage of total 1 74.89180687736872 50.119768971158564 2 15.468451268767518 20.703872326160425 3 5.010269770435678 10.059053473843969 4 2.13554727678762 5.716680667878305 5 0.9835421800276439 3.291074744470573 6 0.5449218773562204 2.1880651360284022 7 0.2993303853952123 1.402244019914625 8 0.2196279550877438 1.1758511727863876 9 0.11887562220328034 0.7159951231779418 >10 0.31803270292593144 3.014101577541542 >50 0.005382080825722925 0.2416360969551834 >100 0.0035100023490060514 0.45492210125977806 >500 0.0 0.0 >1k 7.020004696879409E-4 0.916734588824295 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2341 0.36451399431663356 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2326 0.3621783642804313 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1187 0.18482619019813928 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.5570866908015105E-4 0.0 9 0.0 0.0 0.0 1.5570866908015105E-4 0.0 10-11 0.0 0.0 0.0 1.5570866908015105E-4 0.0 12-13 0.0 0.0 0.0 1.5570866908015105E-4 0.0 14-15 0.0 0.0 0.0 1.5570866908015105E-4 0.0 16-17 0.0 0.0 0.0 1.5570866908015105E-4 0.0 18-19 0.0 0.0 0.0 1.5570866908015105E-4 0.0 20-21 0.0 0.0 0.0 1.5570866908015105E-4 0.0 22-23 0.0 0.0 0.0 1.5570866908015105E-4 0.0 24-25 0.0 0.0 0.0 8.563976799408307E-4 0.0 26-27 0.0 0.0 0.0 0.00225777570166219 0.0 28-29 0.0 0.0 0.0 0.006773327104986571 0.0 30-31 0.0 0.0 0.0 0.01510374090077465 0.0 32-33 0.0 0.0 0.0 0.027716143096266882 0.0 34-35 0.0 0.0 0.0 0.043442718673362135 0.0 36-37 0.0 0.0 0.0 0.06664331036630465 0.0 38-39 0.0 0.0 0.0 0.09615010315699327 0.0 40-41 0.0 0.0 0.0 0.13118455370002724 0.0 42-43 0.0 0.0 0.0 0.16964459496282455 0.0 44-45 0.0 0.0 0.0 0.2137101483125073 0.0 46-47 0.0 0.0 0.0 0.2749036552610067 0.0 48-49 0.0 0.0 0.0 0.3446611390089143 0.0 50-51 0.0 0.0 0.0 0.41325080773872086 0.0 52-53 0.0 0.0 0.0 0.4869010082136323 0.0 54-55 0.0 0.0 0.0 0.5789248316400015 0.0 56-57 0.0 0.0 0.0 0.6850402896181245 0.0 58-59 0.0 0.0 0.0 0.805091673478921 0.0 60-61 0.0 0.0 0.0 0.9387675658842306 0.0 62-63 0.0 0.0 0.0 1.076024757678384 0.0 64-65 0.0 0.0 0.0 1.2362489781618593 0.0 66-67 0.0 0.0 0.0 1.4445871773911012 0.0 68-69 0.0 0.0 0.0 1.682198606407412 0.0 70-71 0.0 0.0 0.0 1.941297831756783 0.0 72-73 0.0 0.0 0.0 2.198995679084433 0.0 74-75 0.0 0.0 0.0 2.4689945112694147 0.0 76-77 0.0 0.0 0.0 2.7590797617657365 0.0 78-79 0.0 0.0 0.0 3.095955467320643 0.0 80-81 0.0 0.0 0.0 3.4753396395344307 0.0 82-83 0.0 0.0 0.0 3.8747323757250185 0.0 84-85 0.0 0.0 0.0 4.283156214722254 0.0 86-87 0.0 0.0 0.0 4.722410370197361 0.0 88-89 0.0 0.0 0.0 5.224492973646308 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1190 0.0 56.680676 1 TAGCCCG 50 0.0016152852 38.0 5 GTATCAA 1920 0.0 36.367188 1 TATCAAC 2405 0.0 28.835758 2 ATCAACG 2390 0.0 28.817991 3 TCAACGC 2410 0.0 28.578838 4 CAACGCA 2455 0.0 27.861506 5 AACGCAG 2520 0.0 27.33135 6 TCAACCG 90 8.943232E-4 26.388887 5 ACGCAGA 2785 0.0 24.389587 7 CGCAGAG 2775 0.0 23.963963 8 GAGTACT 2135 0.0 22.248245 12-13 GTACATG 1530 0.0 21.732025 1 GCAGAGT 3120 0.0 21.009617 9 CAGAGTA 3080 0.0 20.742695 10-11 GTACTTT 2330 0.0 20.284336 14-15 TACATGG 1595 0.0 20.250784 2 AGAGTAC 2750 0.0 19.95 10-11 GTATAGC 195 1.03148395E-5 19.48718 1 GTAGGAC 220 1.3428598E-6 19.431818 3 >>END_MODULE