FastQCFastQC Report
Fri 10 Feb 2017
SRR2031799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642225
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26800.41729923313480477No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26500.41262797306240023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15320.23854568103079138No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT11060.17221378800264703No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14200.055.617831
CATACCG405.3870026E-447.498915
GTATCAA23850.037.103841
TCAACGC26900.032.1368064
ATACCGC600.00395010931.6659436
ATCAACG27650.031.2651063
TATCAAC28050.030.8192562
CAACGCA28150.030.3723055
AACGCAG28350.030.158046
TACACCG804.4920604E-429.686825
ACGCAGA30400.027.968117
CGCAGAG31300.027.1639148
GCAGAGT34300.024.3726189
GAGTACT25200.023.93794312-13
GTACATG13250.022.9804441
CAGAGTA33900.022.97882310-11
GTACTTT26500.022.40514614-15
ACCGTGC1302.9400084E-421.9225738
ACATGGG13200.021.5904123
AGAGTAC30750.021.23935110-11
Produced by FastQC (version 0.11.5)