FastQCFastQC Report
Fri 10 Feb 2017
SRR2031798_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031798_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067390
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55200.26700332303048774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49430.23909373654704724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28630.13848378873845768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.053.1202281
GTATCAA46500.041.779571
TCAACGC52500.035.7380944
CAACGCA53100.035.5131845
ATCAACG52850.035.501423
AACGCAG55850.034.1897966
TATCAAC57750.032.818182
ACGCAGA61150.030.6827497
CGCAGAG62150.030.1126298
GCAGAGT75750.023.9537969
GTACATG39700.022.4937021
AGAGTAC65450.021.88120810-11
GAGTACT53100.021.78201712-13
TACATGG38800.021.6688162
CAGAGTA73200.021.0245910-11
ACATGGG41150.019.2770373
GTACTTT61450.018.70626614-15
AGTACTT59750.018.32426812-13
TATACTG10400.017.355775
CATGGGG26650.017.1106954
Produced by FastQC (version 0.11.5)