FastQCFastQC Report
Fri 10 Feb 2017
SRR2031798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067390
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66540.3218550926530553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60320.29176884864490976No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35470.17156898311397462No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT21830.1055920750318034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28700.058.1546331
GTATCAA50650.042.716051
TCAACGC59800.035.664114
ATCAACG60350.035.2603763
CAACGCA61000.035.0403865
AACGCAG63550.033.7838486
TATCAAC64550.032.8925442
ACGCAGA71100.030.0627787
CGCAGAG71750.029.6580318
GCAGAGT84350.025.0025379
GAGTACT62850.022.93715912-13
CAGAGTA84800.021.64910110-11
AGAGTAC75600.021.58197410-11
GTACATG38200.021.1614151
TACATGG40150.019.638522
GTACTTT74450.019.42713714-15
AGTACTT69550.018.71283712-13
ACATGGG41500.018.312943
GTGTAGC14250.017.6856141
TAAGCGT1700.001788419616.764424
Produced by FastQC (version 0.11.5)