FastQCFastQC Report
Fri 10 Feb 2017
SRR2031797_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031797_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1864489
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94650.5076457946386382No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80770.4332018048913134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48240.2587304081708178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47750.055.7065851
GTATCAA81550.038.733751
TCAACGC101750.030.7173234
ATCAACG102400.030.4759543
CAACGCA102850.030.4349785
AACGCAG105700.029.7941136
TATCAAC105900.029.558432
GTACATG77850.029.103931
TACATGG78350.027.523822
ACGCAGA117250.026.8186597
CGCAGAG119350.026.3467758
ACATGGG81650.025.5387863
GAGTACT78800.024.1718612-13
GCAGAGT132000.023.4620299
AGAGTAC111800.022.96390710-11
CAGAGTA127000.022.25384910-11
CATGGGG46000.022.097744
GTACTTT86700.021.85977614-15
AGTACTT82900.021.2001612-13
CATGGGA36100.020.920974
Produced by FastQC (version 0.11.5)