FastQCFastQC Report
Fri 10 Feb 2017
SRR2031797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1864489
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119310.6399072346364071No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108330.5810171044184225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65120.3492645974312533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49950.062.775131
GTATCAA91850.041.287341
ATCAACG114050.032.942853
TCAACGC114700.032.8389854
CAACGCA115450.032.6667945
TATCAAC116350.032.3324622
AACGCAG117250.032.3273476
ACGCAGA128850.029.3064167
CGCAGAG131500.028.679718
GTACATG68500.027.090171
TACATGG68100.026.2253172
GCAGAGT143650.026.1878329
GAGTACT99650.024.9051412-13
ACATGGG69550.024.7907413
CAGAGTA138900.024.29639410-11
AGAGTAC128100.023.67509710-11
GTACTTT107350.022.83114214-15
AGTACTT103800.022.1705512-13
CATGGGG36000.020.4507484
GTATAGC7300.020.2058221
Produced by FastQC (version 0.11.5)