FastQCFastQC Report
Fri 10 Feb 2017
SRR2031796_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031796_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135737
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62410.29221762791954253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54370.2545725433421812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32100.15029940484245016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32300.058.0882341
GTATCAA57000.040.01
TCAACGC67900.032.6693654
ATCAACG69500.032.0539553
CAACGCA69900.031.7346215
AACGCAG72700.030.708396
TATCAAC73600.030.5264952
ACGCAGA80100.027.6342097
CGCAGAG79750.027.5172428
GTACATG46350.025.51781
GCAGAGT90250.023.8421069
TACATGG48450.023.1372552
AGAGTAC80150.022.10542710-11
GAGTACT65300.021.9314712-13
ACATGGG50300.021.9085463
CAGAGTA87900.021.83162710-11
AGTACTT68750.019.8290912-13
CATGGGG31250.019.6080024
GTACTTT72850.019.56074114-15
GTGTAGC13800.017.2101441
Produced by FastQC (version 0.11.5)