FastQCFastQC Report
Fri 10 Feb 2017
SRR2031796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135737
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76490.3581433481744241No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67770.31731435097111677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39400.18447964332687028No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT27290.12777790523833224No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33350.058.766251
GTATCAA64950.044.3101121
ATCAACG78000.036.476893
TCAACGC78150.036.2853164
CAACGCA80300.035.4912535
TATCAAC81550.035.0637322
AACGCAG81550.035.0637326
ACGCAGA88600.031.9520037
CGCAGAG89400.031.2941638
GCAGAGT100600.027.385189
GAGTACT74700.024.25825312-13
CAGAGTA99350.023.0682710-11
AGAGTAC90500.022.93603910-11
GTACTTT82350.021.91823614-15
AGTACTT80050.020.79755412-13
GTACATG43950.020.348181
TACATGG45750.018.8958262
GTGGTAT15300.018.654621
ACATGGG46900.017.7235533
ACTTTTT102700.017.55201116-17
Produced by FastQC (version 0.11.5)