FastQCFastQC Report
Fri 10 Feb 2017
SRR2031795_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031795_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807208
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12900.15981011090078395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10540.13057353247242345No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9220.11422086996164557No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATA550.002579172834.5454562
TACCCGT650.00584305429.230774
GGTATCA24650.026.9776881
ATATATA908.945231E-426.3888871
GTATCAA31800.025.9905681
TACATGG24500.024.4285722
GTACCGA1000.001653220523.756
TATATAA1000.001653220523.752
GTACATG25400.023.7499981
GTACTAA1201.7102022E-423.7499981
GTATAGA2103.442983E-822.6190471
ACATGGG26700.022.415733
GTATAAA1703.1470609E-622.3529421
CAACGCA35750.022.3216785
TATCAAC36800.022.2010882
TCAACGC36100.022.1052634
AACGCAG36250.021.882766
ATCAACG36700.021.8732973
GTATGAC1100.002876632421.590913
TAATCCG550.002877865521.5909174-75
Produced by FastQC (version 0.11.5)