FastQCFastQC Report
Fri 10 Feb 2017
SRR2031795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807208
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT49560.6139681469955699No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15580.19301097114993904No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT14880.18433910466695078RNA PCR Primer, Index 23 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13400.16600430124577556No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10060.12462710974123149No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT9010.1116193100167491RNA PCR Primer, Index 23 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8490.1051773520579578No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGT851.6317561E-533.5289544
ATATATA902.3909362E-531.7074781
GGTATCA24200.026.5320851
GTATATA950.001217064925.032221
TATACTG1901.1901648E-824.9996595
GCATATA1000.001640872223.7806071
CCTAATA1401.7788178E-523.7496762
ATAGGAC2002.0523657E-823.7496743
GTACATG23250.023.729471
TACATGG23800.023.1509442
ACATGGG24050.023.1077923
CTAGGAC2606.2391337E-1021.9227773
GTATCAA29850.021.4821192
TAATAGA1150.003722626720.6518924
CATGGGG17300.020.5922034
GTCTTAC2151.0647418E-619.9093481
GTATTAC1200.004726937519.8171751
TATAGTG1200.00476266919.7913975
ATACGGT1200.00476266919.7913976
TCTAGGA3603.6379788E-1219.7913952
Produced by FastQC (version 0.11.5)