##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031792_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 88196 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.939271622295795 33.0 31.0 34.0 16.0 34.0 2 29.988242097147264 33.0 31.0 34.0 16.0 34.0 3 29.973218740078917 34.0 31.0 34.0 16.0 34.0 4 33.03182684021951 37.0 35.0 37.0 17.0 37.0 5 33.11942718490634 37.0 35.0 37.0 17.0 37.0 6 33.31670370538347 37.0 35.0 37.0 17.0 37.0 7 33.339992743435076 37.0 35.0 37.0 17.0 37.0 8 33.341092566556306 37.0 35.0 37.0 17.0 37.0 9 34.90303415120867 39.0 37.0 39.0 17.0 39.0 10-11 34.92360764660529 39.0 37.0 39.0 17.0 39.0 12-13 34.83466937276067 39.0 37.0 39.0 17.0 39.0 14-15 35.86533969794549 40.0 37.0 41.0 17.0 41.0 16-17 35.797870651730236 40.0 36.5 41.0 17.0 41.0 18-19 36.100339017642526 40.5 37.5 41.0 17.5 41.0 20-21 36.11252210984625 41.0 37.5 41.0 18.0 41.0 22-23 35.95484489092476 41.0 36.5 41.0 18.0 41.0 24-25 35.78631683976597 40.5 36.0 41.0 17.0 41.0 26-27 35.54484897274253 40.0 35.0 41.0 16.5 41.0 28-29 35.252171300285724 40.0 35.0 41.0 16.0 41.0 30-31 34.65691187808971 39.0 35.0 41.0 15.0 41.0 32-33 33.95405687332759 37.5 35.0 41.0 14.0 41.0 34-35 33.43788833960724 36.5 34.5 41.0 12.5 41.0 36-37 33.02142954328995 35.5 33.5 41.0 11.0 41.0 38-39 32.539661662660436 35.0 33.0 40.0 8.5 41.0 40-41 32.05848337793097 35.0 31.5 40.0 8.0 41.0 42-43 31.399315161685337 35.0 29.0 40.0 8.0 41.0 44-45 30.628412853190618 35.0 23.5 40.0 7.5 41.0 46-47 29.354919724250536 35.0 17.5 40.0 7.0 41.0 48-49 28.190104993423738 35.0 12.5 40.0 7.0 41.0 50-51 26.891389632182864 34.0 10.0 39.0 7.0 40.0 52-53 26.62555558075196 34.5 10.0 39.0 7.0 40.0 54-55 26.799650777813053 35.0 10.0 40.0 7.0 41.0 56-57 26.509972107578577 35.0 10.0 40.0 7.0 41.0 58-59 26.168408998140507 34.0 8.5 39.0 7.0 41.0 60-61 25.816601659939224 33.5 8.0 39.0 7.0 41.0 62-63 25.418017823937596 33.0 8.0 38.0 7.0 41.0 64-65 24.994398838949614 32.5 8.0 37.0 7.0 41.0 66-67 24.560909791827292 31.5 8.0 36.5 7.0 40.0 68-69 24.079130572815096 31.0 7.5 35.5 7.0 39.0 70-71 23.61323075876457 29.5 7.0 35.0 7.0 39.0 72-73 23.169163000589595 29.0 7.0 35.0 7.0 38.0 74-75 22.794474806113655 27.5 7.0 35.0 7.0 37.0 76-77 22.441425915007482 26.5 7.0 35.0 7.0 36.5 78-79 22.115861263549366 25.5 7.0 35.0 7.0 36.0 80-81 21.77286951789197 24.5 7.0 35.0 7.0 35.5 82-83 21.42682207809878 23.5 7.0 35.0 7.0 35.0 84-85 21.096132477663385 22.5 7.0 35.0 5.5 35.0 86-87 20.811567418023493 20.0 7.0 35.0 5.0 35.0 88-89 20.546425007936868 20.0 7.0 35.0 5.0 35.0 90-91 20.302060184135335 19.0 7.0 35.0 5.0 35.0 92-93 20.03910608190848 18.5 7.0 35.0 5.0 35.0 94-95 19.754246224318564 17.0 7.0 35.0 5.0 35.0 96-97 19.546691459930155 16.5 7.0 35.0 5.0 35.0 98-99 19.37020953331217 15.0 7.0 34.5 5.0 35.0 100-101 18.266831829107893 12.5 7.0 32.5 5.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.3787016694366478 1101 2 1.5975932748843 1101 3 1.1910394105996076 1101 4 1.0655385034769864 1101 5 1.1809012457283075 1101 6 1.3205643104712337 1101 7 1.5989491215545684 1101 8 0.956212415606231 1101 9 0.8808954894389842 1101 10-11 0.8331984693473728 1101 12-13 0.9038776779618374 1101 14-15 1.1025229263476461 1101 16-17 1.436311025232854 1101 18-19 1.4205924937945298 1101 20-21 1.3630551905968744 1101 22-23 1.4046203491506617 1101 24-25 1.094993200974642 1101 26-27 1.3632782686919 1101 28-29 1.8834710110836852 1101 30-31 1.3829558869071903 1101 32-33 1.5620858652667309 1101 34-35 1.8300615699839184 1101 36-37 1.893186909104685 1101 38-39 1.6551411889781775 1101 40-41 1.6627288014675763 1101 42-43 2.47172800549426 1101 44-45 2.4284941716238 1101 46-47 1.737530153263119 1101 48-49 1.477553031858406 1101 50-51 0.9762799595503324 1101 52-53 1.0407070507533938 1101 54-55 0.4742661043447107 1101 56-57 1.0650831843482074 1101 58-59 0.25193761666128367 1101 60-61 1.5062630211458377 1101 62-63 1.5327541467057628 1101 64-65 1.7788909318415378 1101 66-67 1.2161393784690944 1101 68-69 0.998778522683974 1101 70-71 0.3181770906729149 1101 72-73 1.0703759576697962 1101 74-75 1.2472383614235625 1101 76-77 1.7841293965789689 1101 78-79 1.5841831813212472 1101 80-81 1.5571819492541827 1101 82-83 1.5950854348711268 1101 84-85 2.2233149651345308 1101 86-87 2.116938207012531 1101 88-89 0.3640918458623936 1101 90-91 0.2645436174358977 1101 92-93 0.2558551148610704 1101 94-95 1.0358837228187063 1101 96-97 0.36894429641400706 1101 98-99 0.062230974382902815 1101 100-101 0.16046681988906997 1102 1 -0.787194581205366 1102 2 -1.3412870538265373 1102 3 -1.1895461055791294 1102 4 -1.6206453691475673 1102 5 -1.2140653695772912 1102 6 -1.3242739021635863 1102 7 -1.864327714603622 1102 8 -0.8344912310296237 1102 9 -1.3558790354711334 1102 10-11 -1.8897085671189942 1102 12-13 -1.4402414797166472 1102 14-15 -1.7261879108377656 1102 16-17 -1.6305867662412084 1102 18-19 -1.3756324676846674 1102 20-21 -1.7035857955356377 1102 22-23 -1.5451742065760996 1102 24-25 -1.7077802967141125 1102 26-27 -1.4252425325407643 1102 28-29 -1.0438530772573529 1102 30-31 -1.0848150427281453 1102 32-33 -1.5790286699156084 1102 34-35 -1.4846533760869605 1102 36-37 -0.8031407745111352 1102 38-39 -0.481736058068563 1102 40-41 -0.4719656001759738 1102 42-43 -0.3306602841821622 1102 44-45 -0.4511925258595042 1102 46-47 0.3016030859801191 1102 48-49 0.25374717669661706 1102 50-51 -0.12284690228839423 1102 52-53 -0.32164528617521526 1102 54-55 -0.12922644830038266 1102 56-57 0.029002033860276555 1102 58-59 0.4022971441394567 1102 60-61 0.31853831647197595 1102 62-63 0.4942333454730985 1102 64-65 0.4887008958887904 1102 66-67 0.28380758596781064 1102 68-69 0.729646524738417 1102 70-71 0.8695381589831364 1102 72-73 1.4129542832989692 1102 74-75 0.9434376423686075 1102 76-77 1.6566255444988442 1102 78-79 2.0412966892822126 1102 80-81 1.0720766590641482 1102 82-83 0.21591234807092263 1102 84-85 -0.4355191386148256 1102 86-87 -1.0766930205169984 1102 88-89 -1.0795393816671357 1102 90-91 -0.7594676819991335 1102 92-93 -0.6823832004958881 1102 94-95 -0.10959650316999259 1102 96-97 -0.16123033121824903 1102 98-99 -0.8667623486782183 1102 100-101 -0.14153626177502687 1103 1 -1.8023460963568816 1103 2 -1.568559781099264 1103 3 -1.5077279237609496 1103 4 -2.2418574903596884 1103 5 -1.9110350665469866 1103 6 -1.5666981445878285 1103 7 -1.6673580176339229 1103 8 -1.304188200726589 1103 9 -1.3104244900165867 1103 10-11 -2.0184964459068695 1103 12-13 -1.7887263282014985 1103 14-15 -1.9004303350801877 1103 16-17 -2.2745261601806064 1103 18-19 -1.6559354979876986 1103 20-21 -2.226313068262904 1103 22-23 -2.3406287520306464 1103 24-25 -2.3744469633807768 1103 26-27 -2.0691819264801623 1103 28-29 -2.104459137863415 1103 30-31 -1.9029968609099654 1103 32-33 -1.5790286699156084 1103 34-35 -1.8634412548748394 1103 36-37 -2.8258680472384015 1103 38-39 -2.1332512095837117 1103 40-41 -1.4113595395699114 1103 42-43 -1.4821754356973145 1103 44-45 -1.299677374344352 1103 46-47 -1.2741544897774588 1103 48-49 -2.1098891869397463 1103 50-51 -1.0622408416823355 1103 52-53 -1.0034634679933951 1103 54-55 -0.25801432708826155 1103 56-57 0.013850518708760973 1103 58-59 -0.41588467404235985 1103 60-61 -0.5147950168613562 1103 62-63 -0.9072818060420502 1103 64-65 -0.8749354677475765 1103 66-67 -0.5798287776685527 1103 68-69 -0.9748989298070363 1103 70-71 -0.6077345682895938 1103 72-73 -0.8597729894283042 1103 74-75 -0.7459562970253337 1103 76-77 -0.34337445550115575 1103 78-79 -0.8071881592026351 1103 80-81 0.09480393179142155 1103 82-83 -0.3522694701108975 1103 84-85 -1.117337320433009 1103 86-87 -0.5994202932442718 1103 88-89 -0.928024230151987 1103 90-91 -0.19128586381731338 1103 92-93 -0.22783774595043482 1103 94-95 -0.6171722607457504 1103 96-97 -0.8733515433394636 1103 98-99 -0.41979265170851576 1103 100-101 -0.6339605041992691 1104 1 -0.8801792221352116 1104 2 -0.36038211401748654 1104 3 -0.921800152901671 1104 4 -1.394410416885215 1104 5 -0.7524630449612673 1104 6 -1.7632527315533757 1104 7 -0.8551952945122991 1104 8 -1.3639640413243477 1104 9 -1.4924502268368443 1104 10-11 -1.4671265828931652 1104 12-13 -1.040282990717067 1104 14-15 -1.3206254367821373 1104 16-17 -1.1504082689394224 1104 18-19 -1.3273759297021073 1104 20-21 -1.5382682363824571 1104 22-23 -1.2374739159990966 1104 24-25 -1.8360492879967936 1104 26-27 -1.2132250979205992 1104 28-29 -2.08785473769737 1104 30-31 -2.3190408625704038 1104 32-33 -1.9807513764328348 1104 34-35 -1.5677791544182185 1104 36-37 -1.6535973955156962 1104 38-39 -2.174554654996747 1104 40-41 -2.265759705613913 1104 42-43 -1.4654672580302304 1104 44-45 -1.175870815606288 1104 46-47 -0.5849681053855953 1104 48-49 -1.2011096103519492 1104 50-51 -0.5454288865142125 1104 52-53 -0.17614922972025582 1104 54-55 -0.8635560456436799 1104 56-57 -1.1508443754381865 1104 58-59 -0.6268643336521578 1104 60-61 -0.2783898694973068 1104 62-63 -0.8108725075779581 1104 64-65 -0.04720611947028175 1104 66-67 -0.5587619449578867 1104 68-69 0.24500559489201024 1104 70-71 0.5891313511790663 1104 72-73 -0.1937289877678623 1104 74-75 0.5677630886118621 1104 76-77 0.8891909243245024 1104 78-79 1.013484319004089 1104 80-81 -0.1624604932812268 1104 82-83 -0.022049461808698823 1104 84-85 0.29227247616308816 1104 86-87 -0.0551073003011453 1104 88-89 0.3906764755350203 1104 90-91 0.933454692430093 1104 92-93 -0.3790415649624741 1104 94-95 -0.5216969597910008 1104 96-97 0.026710723161158967 1104 98-99 1.0330923628203301 1104 100-101 0.949995494140289 1105 1 0.20363476488713772 1105 2 0.33175919816708443 1105 3 -0.1811728998375024 1105 4 0.36460183978385174 1105 5 0.4362536096890537 1105 6 0.4041949973579477 1105 7 0.4470757941683061 1105 8 -0.23959489929118405 1105 9 0.14093117186538962 1105 10-11 0.27097723830365794 1105 12-13 0.006130131128649907 1105 14-15 -0.08603639568624999 1105 16-17 0.2585679387029671 1105 18-19 -0.19381428586648752 1105 20-21 -0.2502365132389883 1105 22-23 -0.26487117627306844 1105 24-25 0.2054972152476111 1105 26-27 0.03109718037789122 1105 28-29 -0.2846824233498566 1105 30-31 -0.0020797955192151107 1105 32-33 0.14605107490097424 1105 34-35 0.936997341616383 1105 36-37 0.995104839523961 1105 38-39 0.37492263411644444 1105 40-41 -0.5433372110212709 1105 42-43 0.48811962012405985 1105 44-45 0.716072226931562 1105 46-47 1.9299922406850634 1105 48-49 1.9397519613856105 1105 50-51 0.8940988712363271 1105 52-53 1.823490280012983 1105 54-55 2.171411510232314 1105 56-57 2.0228218105747935 1105 58-59 2.810590605064501 1105 60-61 2.2607233244464595 1105 62-63 1.3058362162864974 1105 64-65 1.3698811191742735 1105 66-67 1.7517102972915737 1105 68-69 2.4800452488213516 1105 70-71 2.4189799452670258 1105 72-73 1.6603625767598942 1105 74-75 1.6986210554467576 1105 76-77 1.145859994259613 1105 78-79 0.9194864819456896 1105 80-81 0.5782568823496312 1105 82-83 0.23525046609324818 1105 84-85 0.5048716109864486 1105 86-87 0.8429640767716791 1105 88-89 1.0506440314110144 1105 90-91 1.3764972302815721 1105 92-93 1.0590442317210176 1105 94-95 1.1623333213914115 1105 96-97 1.0327489351294368 1105 98-99 1.0672488155961055 1105 100-101 1.0564302454020051 1106 1 0.8924590984483132 1106 2 0.9790592665197835 1106 3 0.6675967515637282 1106 4 1.4919087434065474 1106 5 1.6647225092105842 1106 6 1.461440383550702 1106 7 1.7374038871279822 1106 8 1.163344266805879 1106 9 1.5965019169098227 1106 10-11 1.1860490086385838 1106 12-13 1.0554295159543514 1106 14-15 0.5032493185994582 1106 16-17 0.7146513289968865 1106 18-19 0.9782636362114303 1106 20-21 0.7282323862825493 1106 22-23 0.8325314211295236 1106 24-25 0.7532586643248536 1106 26-27 1.2857964284981946 1106 28-29 1.205064671660395 1106 30-31 1.54397283605973 1106 32-33 1.5887202478333151 1106 34-35 1.3172946758610848 1106 36-37 1.7498895285191765 1106 38-39 1.7606881843556863 1106 40-41 1.5226231444127727 1106 42-43 0.7440150404931636 1106 44-45 1.0823572576902798 1106 46-47 1.055932090309124 1106 48-49 1.0232276961771376 1106 50-51 0.9572396777981886 1106 52-53 1.0203460558161268 1106 54-55 0.6283493092753751 1106 56-57 1.0495648044230492 1106 58-59 1.9553274471697613 1106 60-61 1.9181054160390794 1106 62-63 1.4357917870315404 1106 64-65 0.9194368266247182 1106 66-67 2.406101958262184 1106 68-69 2.363845658937553 1106 70-71 1.7829580724030478 1106 72-73 1.9952053655500528 1106 74-75 1.4997146986456613 1106 76-77 1.8157164535897543 1106 78-79 1.381123529109054 1106 80-81 2.0547606417481283 1106 82-83 2.3382067203652923 1106 84-85 1.5958661427704577 1106 86-87 1.4471165032925235 1106 88-89 1.5708935187657609 1106 90-91 1.3823072097757603 1106 92-93 1.0784393103266225 1106 94-95 1.579797436223945 1106 96-97 1.8117133917254726 1106 98-99 0.8226315907157229 1106 100-101 0.17015204991328403 1107 1 -1.4277776268517002 1107 2 -1.1932241462699587 1107 3 -1.3955299951073243 1107 4 -1.706184302787765 1107 5 -1.275438595507481 1107 6 -2.3651254556733683 1107 7 -2.703990161861004 1107 8 -1.7069499958934493 1107 9 -1.6282227255363395 1107 10-11 -2.103939483881554 1107 12-13 -2.507943819570883 1107 14-15 -1.7679984210027015 1107 16-17 -2.262251514994567 1107 18-19 -2.627358589663956 1107 20-21 -3.1549666931190643 1107 22-23 -2.233225606652816 1107 24-25 -2.464972471627803 1107 26-27 -2.635541727017184 1107 28-29 -3.0171940822439325 1107 30-31 -2.56995121457318 1107 32-33 -2.2367770396892936 1107 34-35 -2.628209187364291 1107 36-37 -2.2029298040469953 1107 38-39 -1.7818894911333878 1107 40-41 -2.3251493362710995 1107 42-43 -2.5800849101890684 1107 44-45 -2.1939044552802933 1107 46-47 -3.1068165534521803 1107 48-49 -3.050337978654362 1107 50-51 -4.054185605778997 1107 52-53 -3.2338048565626316 1107 54-55 -2.815264039401267 1107 56-57 -2.835689182096761 1107 58-59 -2.7725206540960627 1107 60-61 -2.9246530912763937 1107 62-63 -3.0483443300159685 1107 64-65 -3.1497724451161986 1107 66-67 -3.0120305421488 1107 68-69 -3.214158615269252 1107 70-71 -2.719261227284605 1107 72-73 -2.746616104119518 1107 74-75 -2.269067152414671 1107 76-77 -2.11063567529402 1107 78-79 -3.319271013057641 1107 80-81 -2.3005738971307146 1107 82-83 -2.2231939043264717 1107 84-85 -1.811334251771715 1107 86-87 -2.6309699671990074 1107 88-89 -2.345841644804846 1107 90-91 -2.2710710575265587 1107 92-93 -1.7070283865334765 1107 94-95 -2.553689330174212 1107 96-97 -1.8161018310264616 1107 98-99 -2.3189104115857724 1107 100-101 -1.3452186553308394 1108 1 1.9249266309158486 1108 2 1.4579553704158883 1108 3 1.5036357126026871 1108 4 2.0876879641857684 1108 5 1.8124497819378576 1108 6 2.232544279654597 1108 7 1.9841571338812258 1108 8 1.923084526546134 1108 9 2.060787631195538 1108 10-11 2.4587762813658642 1108 12-13 2.2055918536166885 1108 14-15 2.220781786131937 1108 16-17 2.359943536789096 1108 18-19 2.391412986860786 1108 20-21 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2313 38-39 -0.8523226580129588 2313 40-41 -0.5698944297561788 2313 42-43 -0.0556394334732353 2313 44-45 -1.5983290285005936 2313 46-47 -1.7120888795467089 2313 48-49 -1.7656439826027999 2313 50-51 -1.7991048950601503 2313 52-53 -4.830541588649499 2313 54-55 -3.4792403487730006 2313 56-57 -2.2344814245773073 2313 58-59 -1.3107275987017992 2313 60-61 -0.8456262651237978 2313 62-63 -1.109603184968936 2313 64-65 -0.7730450034717862 2313 66-67 -0.9427006153110327 2313 68-69 -0.49943326397439947 2313 70-71 -0.9858969258097474 2313 72-73 -1.1897702093337799 2313 74-75 -1.2639574090631456 2313 76-77 -1.9082201602551123 2313 78-79 -1.4335434552826989 2313 80-81 -1.451137130204689 2313 82-83 -1.9976684136749796 2313 84-85 -1.511485221461644 2313 86-87 -2.3437210994716864 2313 88-89 -2.481888561539254 2313 90-91 -2.0109967339869 2313 92-93 -1.6814796697758432 2313 94-95 -1.2479757080629632 2313 96-97 -0.679648457434542 2313 98-99 -0.6994701607438252 2313 100-101 0.028605523045658998 2314 1 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0.8044653782042452 2314 60-61 0.6291443770780383 2314 62-63 0.2693117153790574 2314 64-65 0.5089464549274503 2314 66-67 0.39417853685600335 2314 68-69 0.20940096569975708 2314 70-71 0.09184745887863954 2314 72-73 1.132912486015794 2314 74-75 0.6500729610728904 2314 76-77 0.8929108109565291 2314 78-79 1.2284701480073998 2314 80-81 -0.10595364396615636 2314 82-83 0.3827096312475149 2314 84-85 0.06748504111348907 2314 86-87 1.345459539566594 2314 88-89 1.161578695657834 2314 90-91 0.8583484099548926 2314 92-93 1.1386721809043188 2314 94-95 1.4234494738414973 2314 96-97 0.9914603688862442 2314 98-99 1.7403431894618073 2314 100-101 1.7612850548393943 2315 1 0.42644178243099873 2315 2 0.4155311279916454 2315 3 0.007423591390566031 2315 4 0.22556675206455168 2315 5 0.17002553951361676 2315 6 -0.02124359913328533 2315 7 0.15233895206304737 2315 8 -0.7966124431508348 2315 9 -0.2217881263802255 2315 10-11 -0.5184964459068695 2315 12-13 -1.385317237292405 2315 14-15 -0.17353639568624857 2315 16-17 0.19365565800121232 2315 18-19 0.2811857141335139 2315 20-21 0.4752020832522419 2315 22-23 0.3892954903936001 2315 24-25 -0.04187120580501613 2315 26-27 0.06377261897438302 2315 28-29 0.3716772257729488 2315 30-31 0.0898061693930643 2315 32-33 -0.3892559426428832 2315 34-35 -0.6467745882081744 2315 36-37 -0.1323074411778009 2315 38-39 -0.26279666412916924 2315 40-41 0.7481101573997826 2315 42-43 0.8170669885451112 2315 44-45 0.3700195953526162 2315 46-47 0.48152732840435775 2315 48-49 0.8882168736663125 2315 50-51 1.322607643166151 2315 52-53 0.41471835018842285 2315 54-55 0.8722887032147675 2315 56-57 -0.44713432977608747 2315 58-59 0.659494113836427 2315 60-61 0.43747771041137185 2315 62-63 0.2332484969882529 2315 64-65 0.17317059285848302 2315 66-67 0.5273681920284155 2315 68-69 0.31601016110205293 2315 70-71 0.22446240140737928 2315 72-73 0.3724239802686675 2315 74-75 0.34116491509588087 2315 76-77 0.7664740293473287 2315 78-79 0.8280391135246354 2315 80-81 0.5690463560338443 2315 82-83 0.4503820450406195 2315 84-85 0.5822838916882027 2315 86-87 0.5377009188769399 2315 88-89 0.2265212243934691 2315 90-91 0.25719898466753577 2315 92-93 0.10663195101926348 2315 94-95 -0.14292983650332758 2315 96-97 -0.1203212403091598 2315 98-99 -0.49327750019336847 2315 100-101 0.2713425261037621 2316 1 -0.06939155090233484 2316 2 0.02108668354719967 2316 3 0.04214581361279102 2316 4 0.3616778631756645 2316 5 0.11169220618028675 2316 6 -0.9517991546888354 2316 7 0.06622784095193168 2316 8 0.18949866796027948 2316 9 0.12265631806422306 2316 10-11 0.1898368874264591 2316 12-13 -0.2221227928479621 2316 14-15 -0.3777030623529143 2316 16-17 -0.7896776753321149 2316 18-19 -0.6278420636442661 2316 20-21 -0.2866034723033124 2316 22-23 0.19346215706026015 2316 24-25 0.12618434975053816 2316 26-27 -0.17997738102562266 2316 28-29 -1.0463783297826055 2316 30-31 -0.9976938306069414 2316 32-33 -0.5774503870873247 2316 34-35 -0.4051079215415072 2316 36-37 -0.7441129967333495 2316 38-39 -1.2301577752402792 2316 40-41 -0.5824453981557731 2316 42-43 0.1469280996562219 2316 44-45 0.5207140397970598 2316 46-47 0.26277732840435775 2316 48-49 0.21113354033298037 2316 50-51 0.17955208761059538 2316 52-53 0.314718350188425 2316 54-55 0.8174275921036589 2316 56-57 0.6480045591128025 2316 58-59 0.2087996693919827 2316 60-61 0.2471999326335954 2316 62-63 -0.3611959474561921 2316 64-65 -0.9219682960303999 2316 66-67 -0.9886040301938053 2316 68-69 -0.7589898388979428 2316 70-71 -1.1033153763704 2316 72-73 -0.6504926863979996 2316 74-75 -0.922723973793012 2316 76-77 -0.9592204150971142 2316 78-79 -0.5448775531420296 2316 80-81 -0.9253980884105992 2316 82-83 -1.1773957327371605 2316 84-85 -0.5232716638673516 2316 86-87 -1.022715747789725 2316 88-89 -0.7790343311620873 2316 90-91 -1.158773237554687 2316 92-93 -1.263506937869625 2316 94-95 -1.210290947614439 2316 96-97 -1.4661545736424912 2316 98-99 -1.955777500193367 2316 100-101 -1.565463029451795 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 23.0 7 769.0 8 1865.0 9 1278.0 10 1048.0 11 1119.0 12 1203.0 13 1087.0 14 873.0 15 825.0 16 1069.0 17 1547.0 18 1898.0 19 2746.0 20 3851.0 21 5051.0 22 4554.0 23 3594.0 24 2617.0 25 1986.0 26 1684.0 27 1492.0 28 1551.0 29 1458.0 30 1534.0 31 1671.0 32 1797.0 33 2059.0 34 2699.0 35 4219.0 36 6825.0 37 10864.0 38 9611.0 39 1727.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.92761576488729 17.285364415619757 13.151390085718173 15.635629733774776 2 19.5054197469273 23.06907342736632 38.2523016916867 19.173205134019682 3 19.265046033833734 33.61376933194249 30.047847974964853 17.073336659258924 4 11.87355435620663 21.859267994013333 34.23284502698535 32.034332622794686 5 11.00730191845435 39.53240509773686 30.03424191573314 19.42605106807565 6 23.739171844528094 38.089028980906164 17.306907342736633 20.86489183182911 7 22.796952242732097 41.77173567962266 17.57562701256293 17.855685065082316 8 28.413987028890197 30.27915098190394 17.716222957957278 23.590639031248585 9 32.36768107397161 13.686561748832146 16.763798811737495 37.18195836545875 10-11 25.621910290716134 28.691777404870972 21.245861490317022 24.440450814095875 12-13 30.782008254342603 30.05124948977278 22.954555762166084 16.212186493718537 14-15 27.06528640754683 26.924123543017824 28.313642342056326 17.696947707379017 16-17 17.762710326998956 31.70608644382965 27.74105401605515 22.79014921311624 18-19 17.344324005623836 25.94392035919996 37.480157830287084 19.231597804889113 20-21 22.424486371264003 23.516939543743483 38.1423193795637 15.916254705428818 22-23 21.21127942310309 25.490951970610915 36.9313801079414 16.366388498344598 24-25 18.482130708875687 26.742142500793687 37.84638759127398 16.92933919905665 26-27 18.08982266769468 26.955304095423827 37.78345956732731 17.171413669554177 28-29 17.71452220055331 27.767699215383917 37.68708331443602 16.83069526962674 30-31 18.208875685972153 27.83062723933058 37.608281554719035 16.35221551997823 32-33 17.103950292530275 28.59936958592226 37.62869064356661 16.66798947798086 34-35 16.954283640981448 29.27457027529593 37.17969068892013 16.591455394802484 36-37 17.411220463513086 29.226382148850288 36.31627284684112 17.0461245407955 38-39 17.420858088802213 29.089187718263865 36.0192072202821 17.47074697265182 40-41 17.190688920132434 29.22411447231167 36.061726155381194 17.523470452174703 42-43 17.184452809651233 29.50927479704295 35.450587328223506 17.855685065082316 44-45 17.16064220599574 29.723003310807744 34.591704839221734 18.524649643974783 46-47 17.377205315433805 29.712798766383962 33.18121003220101 19.728785885981225 48-49 18.106263322599663 30.250238106036555 31.46004353938954 20.18345503197424 50-51 18.788267041589187 30.604562565195703 29.97188081092113 20.635289582293982 52-53 18.494036010703432 30.577350446732275 29.296680121547464 21.63193342101683 54-55 18.641434985713637 30.234364370266224 29.21674452356116 21.907456120458978 56-57 18.681119325139463 29.732640936096878 29.14077735951744 22.445462379246226 58-59 18.52294888657082 29.130572815093654 29.403260918862532 22.94321737947299 60-61 18.53655494580253 29.022291260374622 29.5761712549322 22.86498253889065 62-63 18.544491813687696 28.348791328404914 30.311465372579256 22.795251485328134 64-65 18.557530953784752 27.794911333847338 30.706608009433534 22.940949702934375 66-67 18.4532178330083 28.072134790693454 30.43335298653 23.041294389768243 68-69 18.918091523425097 28.63225089573223 29.830717946392127 22.61893963445054 70-71 18.86423420563291 28.35842895369404 29.559730600027212 23.217606240645836 72-73 19.03317610775999 28.195156242913512 29.474125810694364 23.29754183863214 74-75 19.211755635176196 27.74955780307497 29.558596761757904 23.48008979999093 76-77 19.041112975645156 27.423579300648555 29.5336523198331 24.001655403873194 78-79 19.536033380198646 27.461562882670414 29.001882171527054 24.000521565603883 80-81 20.021883078597668 27.34534446006622 28.014875958093338 24.61789650324278 82-83 20.71579209941494 26.971744750328813 27.50464873690417 24.80781441335208 84-85 20.827475168941902 26.536350854914055 27.171300285727245 25.464873690416802 86-87 21.308789514263683 26.051634994784344 26.87253390176425 25.767041589187716 88-89 22.303732595582566 25.500589595900042 26.200734727198512 25.994943081318883 90-91 22.95512268130074 25.154768923760717 25.881559254387952 26.008549140550592 92-93 24.131479885709105 24.636037915551725 25.915007483332573 25.317474715406597 94-95 24.184203365231983 24.331035421107533 26.29370946528187 25.191051748378612 96-97 24.468229851693955 24.20064402013697 26.269898861626377 25.0612272665427 98-99 25.206358565014288 23.96820717492857 25.792552950247178 25.03288130980997 100-101 27.290353304004718 23.1217969068892 25.566919134654633 24.02093065445145 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 76.0 1 56.5 2 49.5 3 58.5 4 55.0 5 53.0 6 81.5 7 151.5 8 247.5 9 325.0 10 321.0 11 284.5 12 291.5 13 316.0 14 337.5 15 376.0 16 412.5 17 460.5 18 511.5 19 573.0 20 651.0 21 713.5 22 783.5 23 872.0 24 991.0 25 1105.0 26 1259.0 27 1451.0 28 1611.5 29 1770.0 30 1934.0 31 2074.5 32 2224.5 33 2344.0 34 2377.0 35 2418.0 36 2438.0 37 2381.0 38 2213.0 39 2149.5 40 2082.5 41 1890.5 42 1771.0 43 1785.5 44 1841.0 45 1841.0 46 1858.5 47 1903.5 48 2005.5 49 2201.5 50 2399.0 51 2431.0 52 2458.0 53 2280.0 54 1888.5 55 1642.5 56 1516.5 57 1491.0 58 1534.0 59 1569.0 60 1617.0 61 1572.5 62 1398.5 63 1195.0 64 1000.0 65 820.5 66 657.5 67 526.0 68 420.0 69 350.0 70 283.5 71 233.0 72 186.5 73 138.5 74 120.5 75 110.0 76 82.5 77 59.5 78 48.5 79 42.0 80 34.0 81 31.0 82 27.5 83 21.0 84 13.5 85 10.5 86 12.0 87 9.5 88 5.5 89 5.5 90 5.0 91 2.0 92 1.5 93 1.5 94 2.0 95 1.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 88196.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.54773459113792 #Duplication Level Percentage of deduplicated Percentage of total 1 93.26841535807235 72.32754319923806 2 4.400970845395795 6.825706381241779 3 1.046875456911425 2.435484602476303 4 0.4284001520601223 1.3288584516304593 5 0.2105447846302307 0.8163635539026712 6 0.10673450887504753 0.4966211619574584 7 0.09649969295552241 0.5238332804208807 8 0.05702254583735415 0.3537575400244909 9 0.06287101207708279 0.43879541022268587 >10 0.26464309734772057 4.064810195473718 >50 0.036552913998303946 2.1316159463014195 >100 0.016083282159253736 1.783527597623475 >500 0.0 0.0 >1k 0.004386349679796474 6.473082679486598 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2363 2.679259830377795 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2215 2.5114517665200236 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1131 1.2823710825887795 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 221 0.25057825751734775 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.22449997732323462 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 149 0.1689419021270806 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 144 0.16327271078053426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 140 0.1587373577032972 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 131 0.14853281327951381 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 126 0.1428636219329675 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 122 0.13832826885573044 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 120 0.1360605923171119 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 119 0.1349267540478026 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 103 0.11678534173885435 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 99 0.11224998866161731 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 98 0.11111615039230804 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 97 0.10998231212299879 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 96 0.10884847385368951 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 96 0.10884847385368951 No Hit GTACATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA 90 0.1020454442378339 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.669191346546329E-4 0.0 12-13 0.0 0.0 0.0 0.0011338382693092657 0.0 14-15 0.0 0.0 0.0 0.0011338382693092657 0.0 16-17 0.0 0.0 0.0 0.0011338382693092657 0.0 18-19 0.0 0.0 0.0 0.0022676765386185314 0.0 20-21 0.0 0.0 0.0 0.00396843394258243 0.0 22-23 0.0 0.0 0.0 0.02777903759807701 0.0 24-25 0.0 0.0 0.0 0.13095832010522018 0.0 26-27 0.0 0.0 0.0 0.28516032473128033 0.0 28-29 0.0 0.0 0.0 0.5255340378248446 0.0 30-31 0.0 0.0 0.0 1.0300920676674679 0.0 32-33 0.0 0.0 0.0 1.4433761168306953 0.0 34-35 0.0 0.0 0.0 1.8334164814730827 0.0 36-37 0.0 0.0 0.0 2.409973241416844 0.0 38-39 0.0 0.0 0.0 3.2132976552224592 0.0 40-41 0.0 0.0 0.0 4.27060184135335 0.0 42-43 0.0 0.0 0.0 4.869835366683296 0.0 44-45 0.0 0.0 0.0 5.318268402195111 0.0 46-47 0.0 0.0 0.0 5.853440065309084 0.0 48-49 0.0 0.0 0.0 6.455508186312304 0.0 50-51 0.0 0.0 0.0 6.9895460111569685 0.0 52-53 0.0 0.0 0.0 7.417569957821216 0.0 54-55 0.0 0.0 0.0 7.821783300829969 0.0 56-57 0.0 0.0 0.0 8.223162048165449 0.0 58-59 0.0 0.0 0.0 8.658555943580208 0.0 60-61 0.0 0.0 0.0 9.068438477935507 0.0 62-63 0.0 0.0 0.0 9.42729829017189 0.0 64-65 0.0 0.0 0.0 9.769717447503288 0.0 66-67 0.0 0.0 0.0 10.122341149258471 0.0 68-69 0.0 0.0 0.0 10.534491360152387 0.0 70-71 0.0 0.0 0.0 10.95571227720078 0.0 72-73 0.0 0.0 0.0 11.32761122953422 0.0 74-75 0.0 0.0 0.0 11.623543017823938 0.0 76-77 0.0 0.0 0.0 11.919474806113655 0.0 78-79 0.0 0.0 0.0 12.191029071613226 0.0 80-81 0.0 0.0 0.0 12.5 0.0 82-83 0.0 0.0 0.0 12.801600979636264 0.0 84-85 0.0 0.0 0.0 13.138917864755772 0.0 86-87 0.0 0.0 0.0 13.439385006122727 0.0 88-89 0.0 0.0 0.0 13.777268810376889 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAGG 20 0.0020677103 71.25 1 GGTATCA 1215 0.0 57.469135 1 TATAACT 25 0.0050059496 57.0 4 ATGGGAC 120 8.367351E-11 39.583336 5 GTATCAA 2075 0.0 38.457832 1 GTAGAAG 55 0.002566779 34.545456 3 TGGGACC 70 2.0380455E-4 33.92857 6 AACGCAG 2380 0.0 33.32983 6 CAACGCA 2460 0.0 32.439022 5 TCAACGC 2455 0.0 32.31161 4 ATCAACG 2480 0.0 31.985888 3 TATCAAC 2470 0.0 31.923075 2 TATGTGT 60 0.003931365 31.666668 6 CGCAGAG 2580 0.0 30.746126 8 ACGCAGA 2585 0.0 30.502901 7 GCAGAGT 2665 0.0 29.409004 9 GAGTACT 1655 0.0 29.13142 12-13 GTACATG 1465 0.0 28.856655 1 TACATGG 1435 0.0 28.4669 2 CAGAGTA 2635 0.0 27.220114 10-11 >>END_MODULE