##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031792_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 88196 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.60404099959182 34.0 31.0 34.0 31.0 34.0 2 32.66633407410767 34.0 31.0 34.0 31.0 34.0 3 32.6263889518799 34.0 31.0 34.0 30.0 34.0 4 36.16700303868656 37.0 35.0 37.0 35.0 37.0 5 36.21972651820944 37.0 37.0 37.0 35.0 37.0 6 36.36560614993877 37.0 37.0 37.0 35.0 37.0 7 36.29541022268584 37.0 37.0 37.0 35.0 37.0 8 36.348938727379924 37.0 37.0 37.0 35.0 37.0 9 38.09404054605651 39.0 39.0 39.0 37.0 39.0 10-11 38.094964624246 39.0 39.0 39.0 37.0 39.0 12-13 38.05939611773776 39.0 39.0 39.0 35.0 39.0 14-15 39.25440496167627 41.0 39.0 41.0 36.0 41.0 16-17 39.1879450315207 40.5 38.5 41.0 36.0 41.0 18-19 39.498979545557624 41.0 39.5 41.0 36.5 41.0 20-21 39.62559526509139 41.0 40.0 41.0 37.0 41.0 22-23 39.485152387863394 41.0 39.0 41.0 35.5 41.0 24-25 39.31342124359381 41.0 39.0 41.0 35.0 41.0 26-27 38.9879302916232 41.0 38.5 41.0 35.0 41.0 28-29 38.70482221415937 40.5 38.0 41.0 35.0 41.0 30-31 38.13914463240963 39.5 36.5 41.0 35.0 41.0 32-33 36.95025284593406 38.5 35.0 41.0 33.0 41.0 34-35 36.59561658125085 38.0 35.0 41.0 33.0 41.0 36-37 36.289480248537345 38.0 35.0 41.0 32.5 41.0 38-39 35.60835525420654 38.0 35.0 41.0 29.5 41.0 40-41 35.30055217923716 38.0 35.0 41.0 26.5 41.0 42-43 34.73966506417525 38.0 35.0 41.0 21.5 41.0 44-45 33.777127080593225 37.5 34.0 40.0 14.0 41.0 46-47 32.492029116966755 37.0 32.0 40.0 8.5 41.0 48-49 31.344148260692094 37.0 26.5 40.0 7.0 41.0 50-51 30.448727833461835 36.0 22.5 40.0 7.0 41.0 52-53 30.225418386321376 36.0 20.5 40.0 7.0 41.0 54-55 29.909871195972606 36.0 19.0 40.0 7.0 41.0 56-57 29.225594131253118 35.0 18.0 40.0 7.0 41.0 58-59 28.734460746519115 35.0 16.0 39.0 7.0 41.0 60-61 27.97213025534038 35.0 12.5 39.0 7.0 41.0 62-63 27.176221143816047 35.0 10.0 38.5 7.0 41.0 64-65 26.526282371082587 34.0 10.0 37.0 7.0 41.0 66-67 25.694532631865393 33.5 10.0 36.5 7.0 40.0 68-69 24.638776134972105 32.0 8.0 36.0 7.0 39.0 70-71 23.73934758945984 30.0 7.5 35.0 7.0 39.0 72-73 23.033113746655175 28.5 7.0 35.0 7.0 38.0 74-75 22.707617125493222 27.0 7.0 35.0 7.0 37.0 76-77 21.776305047847977 24.5 7.0 34.5 7.0 36.0 78-79 22.294951018186765 26.0 7.0 35.0 7.0 36.0 80-81 22.1940224046442 25.0 7.0 35.0 7.0 36.0 82-83 21.99866774003356 25.0 7.0 35.0 7.0 35.0 84-85 21.78629416300059 24.5 7.0 35.0 7.0 35.0 86-87 21.633526463785206 24.0 7.0 35.0 7.0 35.0 88-89 21.452333439158238 24.0 7.0 35.0 7.0 35.0 90-91 21.245152841398703 23.0 7.0 35.0 7.0 35.0 92-93 21.092300104313118 21.5 7.0 35.0 7.0 35.0 94-95 20.922711914372535 20.0 7.0 35.0 7.0 35.0 96-97 20.77934940360107 20.0 7.0 35.0 7.0 35.0 98-99 20.64302235929067 19.5 7.0 35.0 6.0 35.0 100-101 19.796623429633996 18.0 7.0 34.0 5.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14892087050555602 1101 2 0.02798359577952425 1101 3 0.19447394220197367 1101 4 -0.12936903258253807 1101 5 0.03357124027714775 1101 6 0.020122170109132753 1101 7 0.0871875509499418 1101 8 0.13794511864606562 1101 9 0.2037398773133674 1101 10-11 0.2640027611468554 1101 12-13 -0.18758329612945346 1101 14-15 0.3687865210053758 1101 16-17 0.4667437557783174 1101 18-19 0.366259820222524 1101 20-21 0.1955479796969115 1101 22-23 0.3761610113688576 1101 24-25 0.0023876614025653 1101 26-27 0.3667340286420284 1101 28-29 0.31665424284266663 1101 30-31 0.47940510427520877 1101 32-33 -0.042589156672768524 1101 34-35 0.5692636875832093 1101 36-37 0.8648295737929317 1101 38-39 0.4801738685680661 1101 40-41 0.860113492745036 1101 42-43 1.1576258281370926 1101 44-45 1.1249876012926805 1101 46-47 1.7987470388687257 1101 48-49 1.721208150423319 1101 50-51 1.4052521727868097 1101 52-53 0.8769304496192838 1101 54-55 1.0616481789926304 1101 56-57 0.6049113223702847 1101 58-59 -0.13631577904102699 1101 60-61 -0.3660937868489462 1101 62-63 0.4320196917083621 1101 64-65 0.04276897220234588 1101 66-67 1.3662287952116365 1101 68-69 1.06121995787122 1101 70-71 1.0034466081996811 1101 72-73 2.008776564075635 1101 74-75 2.5809606715051423 1101 76-77 1.4215212670613973 1101 78-79 0.9379714100934038 1101 80-81 1.5644879697235687 1101 82-83 1.1255454783264227 1101 84-85 2.213819684557347 1101 86-87 2.0749131732807164 1101 88-89 2.3844541322062547 1101 90-91 3.152076160776833 1101 92-93 3.5065920461052116 1101 94-95 3.07939597454639 1101 96-97 1.7722554726597544 1101 98-99 1.8024428293205013 1101 100-101 1.3151658586904134 1102 1 0.24984536973514082 1102 2 0.5112912485787007 1102 3 0.2124503161105551 1102 4 0.31978350979034076 1102 5 0.26058716220832423 1102 6 0.2648576606073334 1102 7 -0.016561807036701737 1102 8 0.16157121006876451 1102 9 0.1580285265172705 1102 10-11 0.010535889035921286 1102 12-13 0.2046868630898544 1102 14-15 0.07050711679377031 1102 16-17 0.08602983692880883 1102 18-19 0.0806922804177006 1102 20-21 -0.19736419287627882 1102 22-23 -0.11973010316632582 1102 24-25 0.03371791307179706 1102 26-27 -0.014621903561362615 1102 28-29 -0.2047890031768631 1102 30-31 -0.6807130261819054 1102 32-33 -0.4462871535911006 1102 34-35 -0.8292982025041056 1102 36-37 -0.5680158293914559 1102 38-39 -0.9954296239845704 1102 40-41 -1.473861853942175 1102 42-43 -0.5157177774099111 1102 44-45 -0.4935280638331463 1102 46-47 -0.6659422266679975 1102 48-49 -1.0298190709428852 1102 50-51 -0.9859907650662976 1102 52-53 -1.0845487491480483 1102 54-55 -1.2976224938373626 1102 56-57 -2.3312725502542655 1102 58-59 -2.1427359125798056 1102 60-61 -0.777110736001486 1102 62-63 -0.48207892154279364 1102 64-65 -1.186301392461239 1102 66-67 -1.6850038704278099 1102 68-69 -2.9558575973419288 1102 70-71 -2.013630947013464 1102 72-73 -0.7839815252926279 1102 74-75 -0.7320850398456535 1102 76-77 -0.5755254715107689 1102 78-79 -0.1936413274515374 1102 80-81 -1.9579824976621545 1102 82-83 -2.3237354667172383 1102 84-85 -2.0913934638761447 1102 86-87 -1.8775778385323285 1102 88-89 -1.7112058575215308 1102 90-91 -2.6289972855508523 1102 92-93 -2.7202954731551863 1102 94-95 -2.5640298087099005 1102 96-97 -2.5980578298569377 1102 98-99 -1.473366107505388 1102 100-101 -2.366780725798545 1103 1 0.1892393091290785 1103 2 0.2537154910029429 1103 3 0.015480619140852525 1103 4 -0.013549823542994943 1103 5 0.13937504099619957 1103 6 -0.02302112727144845 1103 7 0.10465031417541582 1103 8 0.14641969491724893 1103 9 0.14287701136575492 1103 10-11 0.02568740418743687 1103 12-13 0.15165656005955697 1103 14-15 0.2447495410361924 1103 16-17 0.01784801874699582 1103 18-19 -0.04809559837018185 1103 20-21 -0.2806975262096074 1103 22-23 -0.33185131528752976 1103 24-25 -0.04203966268577375 1103 26-27 0.07628718734773088 1103 28-29 0.2876352392473862 1103 30-31 -0.24889484436372555 1103 32-33 0.34916739186344614 1103 34-35 -0.10202547523137895 1103 36-37 0.08349932212369993 1103 38-39 -0.07118719974214827 1103 40-41 0.6928048127244892 1103 42-43 0.3933731316810025 1103 44-45 0.5595022391971511 1103 46-47 1.0007244399986632 1103 48-49 0.8110900199662048 1103 50-51 0.5670395379640034 1103 52-53 1.0745421599428582 1103 54-55 0.14934720313233285 1103 56-57 0.3202426012608868 1103 58-59 1.0390822692383779 1103 60-61 0.23804077915002964 1103 62-63 0.2527695633056908 1103 64-65 0.033395577235729945 1103 66-67 -0.04106447648841183 1103 68-69 -0.4179788094631398 1103 70-71 -0.9227218561043742 1103 72-73 -1.0036784949895967 1103 74-75 -0.5805698883304977 1103 76-77 -0.3406769866622845 1103 78-79 -0.2845504183606309 1103 80-81 -0.6094976491773032 1103 82-83 -1.6116142545960237 1103 84-85 -1.3035146759973557 1103 86-87 -0.23363844459293404 1103 88-89 -0.1884785847942574 1103 90-91 -0.13657304312660656 1103 92-93 -0.5309015337612486 1103 94-95 -0.9276661723462638 1103 96-97 -1.279876011675121 1103 98-99 -0.8597297438690212 1103 100-101 -0.4576898167076351 1104 1 0.13963366363302043 1104 2 0.395060447416391 1104 3 0.06259560461200664 1104 4 0.1896465234889675 1104 5 -0.1860712022582618 1104 6 -0.04419173748316041 1104 7 0.17355857486450077 1104 8 0.24915942094464327 1104 9 0.5868371608053593 1104 10-11 0.33992567732981627 1104 12-13 0.042275073965733156 1104 14-15 0.23354157092411043 1104 16-17 0.4906583134750946 1104 18-19 0.1855075564658506 1104 20-21 0.31384377723580315 1104 22-23 0.3424948864559312 1104 24-25 0.3604094863387175 1104 26-27 0.2662000142468557 1104 28-29 0.1545924829169607 1104 30-31 0.42275314235275374 1104 32-33 0.282230903694078 1104 34-35 0.2732339685212182 1104 36-37 0.9451639132403429 1104 38-39 0.9352470053221893 1104 40-41 0.9822402631188467 1104 42-43 1.2140456098877266 1104 44-45 1.5408222890103573 1104 46-47 1.6160212436516304 1104 48-49 2.3700356405556597 1104 50-51 1.1361553536551625 1104 52-53 1.432885871026297 1104 54-55 1.2544738116835994 1104 56-57 1.3435925389943861 1104 58-59 1.495910828806661 1104 60-61 1.9865878941355 1104 62-63 1.422964665007644 1104 64-65 0.6080116004818876 1104 66-67 1.1160546600827779 1104 68-69 1.612531775841969 1104 70-71 0.06845705630741605 1104 72-73 0.04789892302617815 1104 74-75 -0.23166992984149815 1104 76-77 -0.16217968487730872 1104 78-79 -0.5191913482070376 1104 80-81 -0.9350476699328034 1104 82-83 -0.830688559286763 1104 84-85 -0.37231915918539826 1104 86-87 -0.42822125903876085 1104 88-89 -0.9871502327809729 1104 90-91 -0.5684950024458288 1104 92-93 0.11002831264805124 1104 94-95 -0.11145612668416405 1104 96-97 0.314976624273406 1104 98-99 0.4955006421832806 1104 100-101 -0.006984129700576602 1105 1 -0.15326467811175348 1105 2 -0.056093121437250204 1105 3 0.11396546762570381 1105 4 -0.10126912178860437 1105 5 -0.10982751083793119 1105 6 0.02123724593428733 1105 7 0.15409210045930877 1105 8 0.0016828528119887665 1105 9 -0.3343957159069717 1105 10-11 0.058382778988075756 1105 12-13 -0.4946751783774488 1105 14-15 0.24534762716059078 1105 16-17 -0.1547995091636949 1105 18-19 0.09763805394241842 1105 20-21 -0.12559385794804712 1105 22-23 -0.230774760263607 1105 24-25 -0.29901733413713316 1105 26-27 0.03143072801758251 1105 28-29 -0.32161515947669983 1105 30-31 -0.18629516334299012 1105 32-33 -0.20226801483512702 1105 34-35 -0.6895454114355246 1105 36-37 -0.3863970096147398 1105 38-39 -0.048260564973411135 1105 40-41 -0.07733075346370555 1105 42-43 -0.18258380611803915 1105 44-45 -0.22458866989375537 1105 46-47 -0.22734573543992553 1105 48-49 0.6015605143840723 1105 50-51 1.2925180068635242 1105 52-53 0.761743116880659 1105 54-55 1.1112690532120801 1105 56-57 1.3381851849929447 1105 58-59 0.8773996536083963 1105 60-61 1.0452576850511441 1105 62-63 0.24100720285912303 1105 64-65 0.3942408722915509 1105 66-67 0.7121253161750651 1105 68-69 0.058097745560782954 1105 70-71 -0.2957282356897011 1105 72-73 -0.6697470755318626 1105 74-75 -0.09053799040386323 1105 76-77 0.07060690488476595 1105 78-79 -0.2709937995408538 1105 80-81 0.3460446155754866 1105 82-83 0.538904086711792 1105 84-85 0.2778250369213069 1105 86-87 -0.18579155464078312 1105 88-89 0.2513140786826149 1105 90-91 0.4850378021684456 1105 92-93 1.1887954359357202 1105 94-95 1.2280355820396984 1105 96-97 0.9452037331414687 1105 98-99 1.292184131729062 1105 100-101 1.9539927989223465 1106 1 0.19140381129357564 1106 2 0.18228691957437348 1106 3 0.2990304026906401 1106 4 0.18341987342670762 1106 5 0.13504603666719817 1106 6 0.19775809350777251 1106 7 -0.0468648373397329 1106 8 -0.04189199339443661 1106 9 -0.4740061055173612 1106 10-11 0.004042382542408518 1106 12-13 0.03693794534093087 1106 14-15 -0.06693877065212206 1106 16-17 0.30572680662577767 1106 18-19 0.23220743193284932 1106 20-21 0.21822022270814045 1106 22-23 -0.06561754905376915 1106 24-25 -0.31909593974205563 1106 26-27 0.10226121332175353 1106 28-29 -0.15825220664006423 1106 30-31 0.04764195217307332 1106 32-33 0.6511154438114914 1106 34-35 0.7204853472794426 1106 36-37 0.536962525586901 1106 38-39 0.38552275696780924 1106 40-41 0.334579704499383 1106 42-43 -0.07415933585146206 1106 44-45 0.15041133010624463 1106 46-47 0.3849235741977992 1106 48-49 -0.5157498068736217 1106 50-51 -0.3680253971009293 1106 52-53 -0.571561736161037 1106 54-55 -0.13744933366420398 1106 56-57 0.12868415970244484 1106 58-59 0.48496971512582476 1106 60-61 0.19366848477773502 1106 62-63 -0.16065035011422069 1106 64-65 -0.06508927124912134 1106 66-67 -0.06487400029793733 1106 68-69 0.39370950222517465 1106 70-71 0.004767321384804291 1106 72-73 -0.030734772045871495 1106 74-75 -0.058924866685476474 1106 76-77 -0.4012830472683433 1106 78-79 0.021726637916426483 1106 80-81 0.6383378486581925 1106 82-83 0.8764809834992136 1106 84-85 0.4789528564701797 1106 86-87 -0.022599483553971567 1106 88-89 0.39160799529232193 1106 90-91 0.4565005499469841 1106 92-93 0.6725616697019525 1106 94-95 1.0257970311169373 1106 96-97 0.904106672307563 1106 98-99 0.24741311327383286 1106 100-101 0.46979936078153983 1107 1 0.38620900609877395 1107 2 0.3751194035460941 1107 3 0.32599078408139803 1107 4 0.23750502877768298 1107 5 0.23910653313275532 1107 6 0.22093744580104868 1107 7 0.2626863709456515 1107 8 0.06414121390459115 1107 9 0.3333258030803705 1107 10-11 0.2349317463431646 1107 12-13 0.35725686692568104 1107 14-15 0.21828233735379143 1107 16-17 0.45762554080299367 1107 18-19 0.32656876680051283 1107 20-21 0.04429288422383593 1107 22-23 0.26481151555251614 1107 24-25 0.18024648998012083 1107 26-27 -0.09057127064997417 1107 28-29 -0.17937665181514006 1107 30-31 -0.0022511926567787555 1107 32-33 0.6728152629796682 1107 34-35 0.12332933873103258 1107 36-37 0.5160846692660073 1107 38-39 0.4163463637407787 1107 40-41 0.7157238767827963 1107 42-43 0.9319231892184021 1107 44-45 1.7833227225113077 1107 46-47 0.5629607269185044 1107 48-49 0.904108815516647 1107 50-51 1.1142202053978352 1107 52-53 0.7238517111511449 1107 54-55 0.5928646561434583 1107 56-57 0.25091003509287546 1107 58-59 -0.9789461158786139 1107 60-61 -0.7549588372673064 1107 62-63 -0.5980167811515393 1107 64-65 -0.6703827119855958 1107 66-67 -1.2370752168029533 1107 68-69 -0.5491640798889144 1107 70-71 -1.6385829991807057 1107 72-73 -1.864533884325997 1107 74-75 -1.903162907126049 1107 76-77 -2.157324474195846 1107 78-79 -2.4087353052037415 1107 80-81 -1.2043384623725508 1107 82-83 -1.961345746732576 1107 84-85 -2.678370832499077 1107 86-87 -2.689622334121129 1107 88-89 -2.1485859879396365 1107 90-91 -2.319925555593045 1107 92-93 -2.1244957033047847 1107 94-95 -1.1544594212914099 1107 96-97 -1.5293965333475406 1107 98-99 -1.7817665678045813 1107 100-101 -0.9238386467805135 1108 1 -1.5463026485917908E-4 1108 2 0.09462458191203638 1108 3 -0.18148907782884294 1108 4 0.16069260069943425 1108 5 -0.19774617112501147 1108 6 -0.3525665818169088 1108 7 -0.10368301915791278 1108 8 -0.1149439414463842 1108 9 -0.16394117045242496 1108 10-11 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-1.023346382364199 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 5.0 14 37.0 15 112.0 16 413.0 17 928.0 18 1609.0 19 2428.0 20 3249.0 21 4908.0 22 5565.0 23 4383.0 24 3175.0 25 3065.0 26 3547.0 27 3545.0 28 3856.0 29 3643.0 30 1878.0 31 1434.0 32 1484.0 33 1677.0 34 2106.0 35 3376.0 36 5893.0 37 11660.0 38 12197.0 39 2018.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.98324383847787 17.13419687354547 14.256360189358247 20.62619909861841 2 16.395301374211982 20.50773277699669 42.58243004217878 20.514535806612543 3 16.08916504149848 32.71803709918817 36.641117510998235 14.551680348315116 4 8.977731416390766 24.769830831330218 31.02521656310944 35.227221189169576 5 8.548006712322556 37.94729919724251 34.65803437797633 18.846659712458617 6 21.410268039366866 39.76824345775319 17.321647240237652 21.499841262642295 7 18.627828926481925 40.53811964261418 16.744523561159237 24.08952786974466 8 26.644065490498438 32.063812417796726 19.65848791328405 21.63363417842079 9 27.325502290353302 11.015238786339516 15.446278742800127 46.21298018050705 10-11 23.104222413714908 27.74445553086308 23.207968615356705 25.94335344006531 12-13 28.58406276928659 27.536963127579483 24.114472311669463 19.764501791464465 14-15 25.053290398657534 25.12982448183591 26.91221824119008 22.904666878316476 16-17 18.12894008798585 32.090457617125494 26.570366002993335 23.210236291895324 18-19 17.694680030840402 22.299764161639985 36.54417433897229 23.461381468547327 20-21 20.279831284865526 20.970905710009525 37.931992380606836 20.81727062451812 22-23 18.670347861581025 26.69848972742528 37.10429044401107 17.52687196698263 24-25 19.019003129393624 27.850469409043495 37.698988616263776 15.431538845299105 26-27 18.819447593995193 28.398680212254522 37.98074742618713 14.801124767563154 28-29 15.783028708784979 24.514150301600978 39.16844301328858 20.53437797632546 30-31 19.636944986167173 26.40425869653952 39.085105900494355 14.873690416798949 32-33 18.606852918499705 26.955871014558486 40.52621434078643 13.91106172615538 34-35 16.29098825343553 28.237675178012605 40.8714680937911 14.599868474760761 36-37 17.808630776905982 26.771055376661074 38.14402013696767 17.276293709465282 38-39 15.360673953467277 24.63660483468638 40.41623202866343 19.586489183182913 40-41 14.25461472175609 24.4863712640029 41.91913465463286 19.339879359608144 42-43 16.947009768072068 23.71662953325283 41.66539109138221 17.67096960729289 44-45 14.17524604290444 26.657671549730143 40.30341512086716 18.863667286498252 46-47 18.15615220644927 23.877500113383824 35.61272620073473 22.353621479432174 48-49 17.72926209805433 24.499410404099958 34.790126536350854 22.981200961494853 50-51 16.70710689827203 26.456982176062404 34.4471404598848 22.38877046578076 52-53 16.00809560524287 28.191754728105582 36.12748877500113 19.672660891650416 54-55 15.627692865889609 28.68724205179373 32.315524513583384 23.369540568733278 56-57 18.03199691595991 30.556374438750055 28.313075422921674 23.098553222368363 58-59 14.349857136378066 32.110299786838404 32.77189441698036 20.767948659803164 60-61 17.707152251802803 34.8995419293392 26.814141230894826 20.579164587963174 62-63 14.364030114744434 37.35600253979772 27.42131162410994 20.85865572134791 64-65 14.171277608961857 36.646786702344784 27.6004580706608 21.58147761803256 66-67 14.45643793369314 36.16830695269627 27.547734591137917 21.827520522472675 68-69 15.331847996779882 35.38899376952339 27.287106485024747 21.992051748671983 70-71 15.661708013968886 34.29293845525874 27.204748514671866 22.840605016100504 72-73 15.79550092974738 32.72994240101592 27.390131071703934 24.084425597532768 74-75 15.804571635901855 31.663567508730555 27.27221189169577 25.25964896367182 76-77 16.221824119007664 31.17091478071568 27.407705564878228 25.19955553539843 78-79 16.668556397115516 30.66805750827702 26.715497301464918 25.94788879314255 80-81 16.624336704612457 30.35228355027439 26.118531452673587 26.904848292439564 82-83 16.481473082679486 29.719034876865162 25.813528958229398 27.98596308222595 84-85 16.71844528096512 28.737697854777995 25.555580751961536 28.988276112295342 86-87 17.090344233298563 28.310807746383055 25.610004988888385 28.988843031429994 88-89 17.92948619827542 28.01843631477797 24.826663491901517 29.225413995045095 90-91 18.282575173477255 27.657149984126267 24.52548868429407 29.53478615810241 92-93 17.956596671050843 27.423579300648555 24.491473536214794 30.128350492085808 94-95 18.535988026667876 26.98591772869518 24.965984851920723 29.512109392716223 96-97 18.741779672547505 27.21495305909565 25.067463377023902 28.97580389133294 98-99 19.358020771917094 27.49160959680711 24.492607374484106 28.657762256791692 100-101 19.88752324368452 26.9394303596535 24.555535398430766 28.61751099823121 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 88.0 1 61.5 2 45.0 3 58.5 4 50.5 5 48.0 6 78.5 7 149.5 8 274.0 9 357.5 10 302.5 11 266.0 12 296.5 13 313.5 14 336.0 15 391.0 16 439.0 17 460.5 18 513.5 19 551.0 20 612.5 21 697.0 22 737.5 23 802.0 24 936.5 25 1034.5 26 1131.5 27 1314.0 28 1450.5 29 1584.5 30 1754.0 31 1885.0 32 1995.0 33 2172.0 34 2330.0 35 2429.5 36 2532.5 37 2673.5 38 2786.5 39 2871.5 40 2882.5 41 2769.5 42 2684.5 43 2625.0 44 2574.0 45 2471.5 46 2404.5 47 2364.0 48 2327.5 49 2369.5 50 2469.0 51 2558.5 52 2404.0 53 1901.0 54 1404.0 55 1197.0 56 1122.5 57 1121.0 58 1193.5 59 1271.0 60 1314.5 61 1203.5 62 1008.0 63 823.5 64 687.5 65 540.0 66 396.5 67 322.5 68 248.0 69 188.5 70 146.0 71 104.0 72 76.5 73 68.5 74 52.5 75 43.5 76 35.0 77 21.0 78 10.5 79 6.5 80 5.5 81 4.0 82 1.5 83 1.5 84 2.0 85 2.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12358837135470996 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 5.669191346546329E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 5.669191346546329E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 5.669191346546329E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 88196.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.90076647467006 #Duplication Level Percentage of deduplicated Percentage of total 1 91.0979228486647 56.390312485827025 2 5.616001758434993 6.952696267404418 3 1.4562039784591714 2.704204272302599 4 0.6099571381470491 1.510272574719942 5 0.2912407956918343 0.9014014241008662 6 0.17218009305051837 0.6394847838904258 7 0.12272410887643331 0.5317701483060456 8 0.08059493717258306 0.39911107079686153 9 0.06410960911455471 0.3571590548324187 >10 0.38465765468732827 4.453716721846796 >50 0.05495109352676118 2.5057825751734772 >100 0.03480235923361542 3.885663748922854 >500 0.0018317031175587059 0.6769014467776316 >1k 0.012821921822910943 18.091523425098643 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 4813 5.457163590185496 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2641 2.9944668692457705 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2546 2.8867522336613907 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 1596 1.8096058778175879 RNA PCR Primer, Index 23 (95% over 23bp) TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 1538 1.7438432581976506 RNA PCR Primer, Index 23 (96% over 30bp) ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT 1484 1.6826159916549503 RNA PCR Primer, Index 23 (96% over 25bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1338 1.5170756043357976 No Hit CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT 597 0.6769014467776316 RNA PCR Primer, Index 23 (96% over 28bp) CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA 448 0.507959544650551 RNA PCR Primer, Index 23 (96% over 30bp) CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG 311 0.35262370175518165 Illumina PCR Primer Index 1 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 304 0.3446868338700168 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 236 0.26758583155698673 No Hit ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC 232 0.26305047847974966 Illumina PCR Primer Index 1 (95% over 24bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 163 0.18481563789741032 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 156 0.17687877001224545 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 154 0.1746110934736269 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 154 0.1746110934736269 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 149 0.1689419021270806 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 148 0.16780806385777133 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 133 0.15080048981813235 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 126 0.1428636219329675 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 126 0.1428636219329675 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 125 0.1417297836636582 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 122 0.13832826885573044 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 120 0.1360605923171119 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC 117 0.1326590775091841 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 103 0.11678534173885435 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 100 0.11338382693092658 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 99 0.11224998866161731 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 98 0.11111615039230804 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 98 0.11111615039230804 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96 0.10884847385368951 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 93 0.10544695904576172 No Hit GTACATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA 93 0.10544695904576172 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 92 0.10431312077645244 No Hit TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC 90 0.1020454442378339 No Hit TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT 90 0.1020454442378339 RNA PCR Primer, Index 23 (95% over 22bp) ATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTT 89 0.10091160596852465 Illumina PCR Primer Index 1 (96% over 26bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.669191346546329E-4 0.0 12-13 0.0 0.0 0.0 0.0011338382693092657 0.0 14-15 0.0 0.0 0.0 0.0011338382693092657 0.0 16-17 0.0 0.0 0.0 0.0011338382693092657 0.0 18-19 0.0 0.0 0.0 0.0022676765386185314 0.0 20-21 0.0 0.0 0.0 0.00396843394258243 0.0 22-23 0.0 0.0 0.0 0.02551136105945848 0.0 24-25 0.0 0.0 0.0 0.13039140097056556 0.0 26-27 0.0 0.0 0.0 0.2834595673273165 0.0 28-29 0.0 0.0 0.0 0.5249671186901901 0.0 30-31 0.0 0.0 0.0 1.0334935824753957 0.0 32-33 0.0 0.0 0.0 1.4592498526010251 0.0 34-35 0.0 0.0 0.0 1.8611955190711598 0.0 36-37 0.0 0.0 0.0 2.4513583382466324 0.0 38-39 0.0 0.0 0.0 3.2575173477255204 0.0 40-41 0.0 0.0 0.0 4.334663703569323 0.0 42-43 0.0 0.0 0.0 4.948637126400291 0.0 44-45 0.0 0.0 0.0 5.396503242777451 0.0 46-47 0.0 0.0 0.0 5.935643339834006 0.0 48-49 0.0 0.0 0.0 6.541112975645154 0.0 50-51 0.0 0.0 0.0 7.083087668374983 0.0 52-53 0.0 0.0 0.0 7.528686108213524 0.0 54-55 0.0 0.0 0.0 7.934600208626241 0.0 56-57 0.0 0.0 0.0 8.341648147308266 0.0 58-59 0.0 0.0 0.0 8.782144314934918 0.0 60-61 0.0 0.0 0.0 9.196562202367454 0.0 62-63 0.0 0.0 0.0 9.562791963354346 0.0 64-65 0.0 0.0 0.0 9.913147988570909 0.0 66-67 0.0 0.0 0.0 10.269740124268674 0.0 68-69 0.0 0.0 0.0 10.683591092566557 0.0 70-71 0.0 0.0 0.0 11.10764660528822 0.0 72-73 0.0 0.0 0.0 11.484080910698896 0.0 74-75 0.0 0.0 0.0 11.787949566873781 0.0 76-77 0.0 0.0 0.0 12.084448274298154 0.0 78-79 0.0 0.0 0.0 12.359404054605651 0.0 80-81 0.0 0.0 0.0 12.668374982992425 0.0 82-83 0.0 0.0 0.0 12.977345911379201 0.0 84-85 0.0 0.0 0.0 13.31239511996009 0.0 86-87 0.0 0.0 0.0 13.616830695269627 0.0 88-89 0.0 0.0 0.0 13.961517529139645 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1230 0.0 58.345757 1 ATATTAG 25 0.0049948287 57.032013 1 GTATCAA 2045 0.0 38.1143 1 ATGGGAG 105 4.5749402E-8 36.19026 5 TGGGGCA 110 6.888513E-8 34.545246 6 AACGCAG 2365 0.0 32.73765 6 TATCAAC 2400 0.0 32.260223 2 TCAACGC 2420 0.0 31.993612 4 ATCAACG 2435 0.0 31.796524 3 CAACGCA 2455 0.0 31.53749 5 TATACAC 1820 0.0 29.752567 3 CTTATAC 1790 0.0 29.737362 1 ACGCAGA 2605 0.0 29.721512 7 ATGGGGC 130 2.9836156E-7 29.230595 5 CGCAGAG 2610 0.0 29.1186 8 TTATACA 1860 0.0 28.857353 2 GAGTACT 1845 0.0 28.705791 12-13 CATGGGG 530 0.0 28.679075 4 GCAGAGT 2690 0.0 28.429201 9 TACACAT 1905 0.0 28.175686 5 >>END_MODULE