FastQCFastQC Report
Fri 10 Feb 2017
SRR2031791_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031791_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15282
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1961.2825546394450988No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.8048684727129958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1220.7983248265933779No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.7525193037560529No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.6674519042010209No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.5889281507656066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.40570605941630683No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.3206386598612747No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.2552021986650962No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.19630938358853553No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT290.1897657374689177No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.1635911529904463No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG250.1635911529904463No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG230.15050386075121058No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT220.14396021463159273No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA200.130872922392357No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.11778563015312131No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG170.11124198403350348No Hit
TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.10469833791388562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1100.056.1363641
CGCAGAA352.701414E-454.2857178
GCAGAAA405.221676E-447.4999969
CAGAAAA401.0436594E-535.62510-11
GTATCAA1800.034.3055531
AACGCAG1950.031.6666686
CAACGCA1950.031.6666685
TATCAAC2100.031.6666663
ACGCAGA2050.030.121957
TCAACGC2050.030.121954
ATCAACG2101.8189894E-1229.4047623
GTACTTT1004.6445166E-528.5000023
AGTACTT1108.889793E-525.9090922
GAGTACT1108.889793E-525.9090921
CGCAGAG1751.2965029E-724.4285728
CAGAGTA1600.023.74999810-11
GCAGAGT1601.6676477E-623.7499989
TACTTTT1302.7615728E-421.9230774
GGGAAGC550.002814330921.5909120-21
GAGTACA857.635045E-519.55882512-13
Produced by FastQC (version 0.11.5)