FastQCFastQC Report
Fri 10 Feb 2017
SRR2031791_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031791_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15282
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT10366.779217379924093No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT2511.6424551760240806RNA PCR Primer, Index 23 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1971.2890982855647166No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT1551.014265148540767RNA PCR Primer, Index 23 (96% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1531.0011778563015312No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG1510.9880905640622955RNA PCR Primer, Index 23 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.8441303494307029No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1250.8179557649522313No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.6739955503206387No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.4907734589713388No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT740.484229812851721RNA PCR Primer, Index 23 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT690.4515115822536317No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.34681324433974614No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA320.20939667582777124RNA PCR Primer, Index 23 (96% over 30bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG310.20285302970815342Illumina PCR Primer Index 1 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGT310.20285302970815342No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.19630938358853553No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT270.17667844522968199No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG260.17013479911006413No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC250.1635911529904463Illumina PCR Primer Index 1 (95% over 24bp)
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT240.15704750687082844No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC240.15704750687082844No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT230.15050386075121058No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.15050386075121058No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG200.130872922392357No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA190.12432927627273917No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT190.12432927627273917RNA PCR Primer, Index 23 (95% over 22bp)
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG190.12432927627273917No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC190.12432927627273917No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC180.11778563015312131No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.11124198403350348No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA170.11124198403350348No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA170.11124198403350348No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG170.11124198403350348No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCTTGTTTGCCGAGT160.10469833791388562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAA301.3035497E-679.166679
CAGTACG250.004927038757.0000049
GAGTACT752.1300366E-950.6666681
AGTACTT851.1277734E-1050.294122
GCAGAAA252.7622847E-547.50000410-11
CAGAAAA252.7622847E-547.50000410-11
GTACTTT1101.4078978E-938.8636363
GGTATCA1101.4078978E-938.8636361
AGTACAT756.484428E-638.07
GAGTACA905.2229734E-736.9444436
TATACAC2850.036.6666643
AACGCAG1301.72804E-1036.5384647
CAACGCA1301.72804E-1036.5384646
TTATACA2900.036.034482
CTTATAC2650.035.849061
TCAACGC1352.6011548E-1035.1851845
ATACACA3000.034.8333364
TACACAT3050.034.26235
ACTTTTT1254.9167284E-934.1999975
TACTTTT1254.9167284E-934.1999974
Produced by FastQC (version 0.11.5)