FastQCFastQC Report
Fri 10 Feb 2017
SRR2031789_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031789_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529224
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84680.3348062488731722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64920.2566795190936034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39180.15490917372284937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39350.057.4586221
GTATCAA77450.043.605491
TCAACGC88300.037.601874
ATCAACG88950.037.3270953
CAACGCA89250.037.0951845
AACGCAG91300.036.4703756
TATCAAC92450.036.222232
ACGCAGA101450.032.7278987
CGCAGAG103850.031.9258088
GCAGAGT119600.027.284669
GTACATG70500.024.1879081
GAGTACT77550.023.8877812-13
CAGAGTA113150.023.7184810-11
TACATGG71300.023.2503172
ACATGGG71550.022.1068843
AGAGTAC108500.021.75803410-11
GTACTTT86850.021.1931214-15
AGTACTT84900.019.6377812-13
TACCGTA2302.0598745E-618.586937
CATGGGG41600.018.4975684
Produced by FastQC (version 0.11.5)