FastQCFastQC Report
Fri 10 Feb 2017
SRR2031789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2529224
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108920.4306459214367727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85960.3398670896686098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51480.20354069074150805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43350.060.565851
GTATCAA79250.042.072331
ATCAACG95850.034.490713
TCAACGC96300.034.329544
CAACGCA97550.033.7435655
AACGCAG100000.032.9643486
TATCAAC100600.032.909392
ACGCAGA110500.029.7030337
CGCAGAG112950.028.9746388
GCAGAGT126700.025.6802359
GTACATG63550.024.5491311
TACATGG64800.023.2364232
CAGAGTA123000.023.17027510-11
GAGTACT93550.022.92445412-13
ACATGGG63800.022.4838813
AGAGTAC116150.022.3488910-11
GTACTTT103150.020.95208214-15
AGTACTT100550.019.69876712-13
GTATAGA10750.018.5831221
CATGGGG36500.018.3489534
Produced by FastQC (version 0.11.5)