FastQCFastQC Report
Fri 10 Feb 2017
SRR2031788_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031788_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703565
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50700.29761118595416086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44730.2625670285548247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27990.16430250680191247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.063.2598651
GTATCAA42550.041.081081
TCAACGC49950.033.4734734
CAACGCA51250.032.995125
AACGCAG52100.032.82156
ATCAACG51200.032.7490233
TATCAAC53500.031.3411222
ACGCAGA58950.028.846487
CGCAGAG58700.028.5647358
GCAGAGT68400.024.4444459
GTACATG35000.023.0714281
AGAGTAC62050.022.81224810-11
TACATGG34800.022.6580452
CAGAGTA67100.021.94485910-11
ACATGGG35300.021.9334283
GAGTACT50300.021.34194812-13
AGTACTT54750.020.17123212-13
CATGGGG20650.020.0121084
TATGCCG1250.006032312319.05
GTACTTT56150.018.86464914-15
Produced by FastQC (version 0.11.5)