##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031788_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1703565 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.62538676246577 34.0 31.0 34.0 31.0 34.0 2 32.755340124973216 34.0 33.0 34.0 31.0 34.0 3 32.83041562840279 34.0 33.0 34.0 31.0 34.0 4 36.0170865215005 37.0 37.0 37.0 35.0 37.0 5 36.04724680302777 37.0 37.0 37.0 35.0 37.0 6 36.118684640738685 37.0 37.0 37.0 35.0 37.0 7 36.13835985125311 37.0 37.0 37.0 35.0 37.0 8 36.13419035962819 37.0 37.0 37.0 35.0 37.0 9 37.97556946755774 39.0 39.0 39.0 37.0 39.0 10-11 38.01281371711676 39.0 39.0 39.0 37.0 39.0 12-13 37.9875880873345 39.0 39.0 39.0 37.0 39.0 14-15 39.57783647820893 41.0 40.0 41.0 37.0 41.0 16-17 39.51839407360447 41.0 40.0 41.0 37.0 41.0 18-19 39.52597875631396 41.0 40.0 41.0 37.0 41.0 20-21 39.472201823822395 41.0 40.0 41.0 37.0 41.0 22-23 39.39093107688876 41.0 40.0 41.0 37.0 41.0 24-25 39.35792646596989 41.0 40.0 41.0 37.0 41.0 26-27 39.24673581577457 41.0 40.0 41.0 36.0 41.0 28-29 39.12902765670814 41.0 39.0 41.0 36.0 41.0 30-31 38.98380777956814 41.0 39.0 41.0 35.5 41.0 32-33 38.86310120247833 41.0 39.0 41.0 35.0 41.0 34-35 38.6758471205971 41.0 39.0 41.0 35.0 41.0 36-37 38.5613255144359 40.0 38.0 41.0 35.0 41.0 38-39 38.37880650283376 40.0 38.0 41.0 34.5 41.0 40-41 38.30213640219188 40.0 38.0 41.0 34.0 41.0 42-43 38.14721305027986 40.0 38.0 41.0 34.0 41.0 44-45 38.29533771825554 40.0 38.0 41.0 34.0 41.0 46-47 38.178167548640644 40.0 38.0 41.0 33.5 41.0 48-49 38.01676748465718 40.0 38.0 41.0 33.0 41.0 50-51 36.9802291077828 39.0 37.0 40.0 31.5 40.5 52-53 37.01046951539859 39.5 37.5 40.0 32.5 40.5 54-55 37.79711927634108 40.0 38.0 41.0 33.0 41.0 56-57 37.80293942409007 40.5 38.0 41.0 33.0 41.0 58-59 37.695524679128766 40.0 37.5 41.0 33.0 41.0 60-61 37.51368101598706 40.0 37.0 41.0 33.0 41.0 62-63 37.267506082832185 40.0 36.5 41.0 32.0 41.0 64-65 36.968834767091366 39.5 36.0 41.0 32.0 41.0 66-67 36.7108868754641 39.0 35.5 41.0 31.0 41.0 68-69 36.395052140657974 39.0 35.0 41.0 31.0 41.0 70-71 36.05336456196271 37.5 35.0 40.5 31.0 41.0 72-73 35.648601608978815 37.0 35.0 39.5 30.0 41.0 74-75 35.288264903305716 37.0 35.0 39.0 30.0 41.0 76-77 34.90395758306845 36.0 35.0 39.0 30.0 41.0 78-79 34.5151725939427 36.0 35.0 38.0 29.5 39.5 80-81 34.14509513872379 35.0 35.0 37.0 29.0 39.0 82-83 33.84238435281307 35.0 35.0 37.0 29.0 39.0 84-85 33.5165350309498 35.0 34.0 36.0 29.0 37.5 86-87 33.24105537505173 35.0 34.0 36.0 28.5 37.0 88-89 33.0249611843399 35.0 34.0 36.0 27.5 37.0 90-91 32.845458200890484 35.0 34.0 35.0 27.0 36.0 92-93 32.70812003064162 35.0 34.0 35.0 27.0 36.0 94-95 32.54155403521439 35.0 34.0 35.0 26.5 36.0 96-97 32.44068027929666 35.0 34.0 35.0 26.0 36.0 98-99 32.32559397498775 35.0 34.0 35.0 25.0 35.5 100-101 30.866512284532728 34.0 31.5 35.0 21.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.32253907808415505 1101 2 0.38841210512817526 1101 3 0.3748468192867236 1101 4 0.32138337019695484 1101 5 0.302130633936045 1101 6 0.3447441889157403 1101 7 0.3212347850744308 1101 8 0.34777072947996857 1101 9 0.35830513344205883 1101 10-11 0.3362869483087323 1101 12-13 0.37599498782728347 1101 14-15 0.4268796163575743 1101 16-17 0.5277508501861021 1101 18-19 0.4593295943565039 1101 20-21 0.4856815884250807 1101 22-23 0.4304198031370703 1101 24-25 0.37423972519530224 1101 26-27 0.40674425991403496 1101 28-29 0.5617982463070845 1101 30-31 0.5364055960418739 1101 32-33 0.5237661608723485 1101 34-35 0.5837583784890157 1101 36-37 0.5169433489833963 1101 38-39 0.5514954840078374 1101 40-41 0.6012236253207561 1101 42-43 0.5578331656190656 1101 44-45 0.6408644112883266 1101 46-47 0.8026563088326384 1101 48-49 0.7598088164885652 1101 50-51 0.7895526277797771 1101 52-53 0.831893729783296 1101 54-55 0.8005561457817478 1101 56-57 0.7412291501561725 1101 58-59 0.7197271625612629 1101 60-61 0.7637180701663695 1101 62-63 0.7928190058934277 1101 64-65 0.8831794512545414 1101 66-67 0.8576530846943484 1101 68-69 0.8135467190146599 1101 70-71 0.7956852194816548 1101 72-73 0.8327964929668781 1101 74-75 0.9194463551819752 1101 76-77 0.7262897304447122 1101 78-79 0.8504385900937379 1101 80-81 0.8420032299461511 1101 82-83 0.8366974457825478 1101 84-85 0.8132403444299783 1101 86-87 0.7630350853614871 1101 88-89 0.7309063246889167 1101 90-91 0.6965559930062071 1101 92-93 0.5800423966305601 1101 94-95 0.7844775615468009 1101 96-97 0.6309004635447835 1101 98-99 0.575987939104543 1101 100-101 0.5856731178652055 1102 1 0.1284537701094024 1102 2 0.10742741330327021 1102 3 0.14378331507861475 1102 4 0.11365277155314857 1102 5 0.1162817750234808 1102 6 0.13100533053923158 1102 7 0.08125310875047376 1102 8 0.18875490148427332 1102 9 0.2976642704532324 1102 10-11 0.26504932585984875 1102 12-13 0.3190930427777374 1102 14-15 0.3198897079163885 1102 16-17 0.2966711015843302 1102 18-19 0.42220303266111614 1102 20-21 0.436074729825485 1102 22-23 0.4464895695589348 1102 24-25 0.42574474119331995 1102 26-27 0.3469578520313874 1102 28-29 0.42769157153988147 1102 30-31 0.42865978181600894 1102 32-33 0.37900181016889434 1102 34-35 0.43126873564367685 1102 36-37 0.4447159014812172 1102 38-39 0.5753094943641699 1102 40-41 0.370672360992522 1102 42-43 0.4594752572255274 1102 44-45 0.4336287251711539 1102 46-47 0.5103695801232178 1102 48-49 0.41933444770509 1102 50-51 0.45241621115223296 1102 52-53 0.5557818145747078 1102 54-55 0.636468818087387 1102 56-57 0.529869553970137 1102 58-59 0.5012162428091145 1102 60-61 0.5267711173168053 1102 62-63 0.418197026470736 1102 64-65 0.46814521078012206 1102 66-67 0.386064529194563 1102 68-69 0.331856045841576 1102 70-71 0.44216470741504565 1102 72-73 0.48120527338653574 1102 74-75 0.4228362893975941 1102 76-77 0.3356375126247215 1102 78-79 0.40377705665546415 1102 80-81 0.41565260337281273 1102 82-83 0.455713349585416 1102 84-85 0.41501011697056356 1102 86-87 0.42191581414996193 1102 88-89 0.4447640378789899 1102 90-91 0.37084317547569157 1102 92-93 0.3440324745549148 1102 94-95 0.3875290167466616 1102 96-97 0.3896444145387008 1102 98-99 0.3629191619662606 1102 100-101 0.31402570351241366 1103 1 0.13089371250408988 1103 2 0.11890923871151671 1103 3 0.24439933150571846 1103 4 0.2529590954943757 1103 5 0.19392687457574453 1103 6 0.22146339738987564 1103 7 0.22251400238845775 1103 8 0.24030903870524156 1103 9 0.24180717347605452 1103 10-11 0.2314837693015761 1103 12-13 0.27596026718704536 1103 14-15 0.2570862591544554 1103 16-17 0.32421091595107754 1103 18-19 0.31071844615069466 1103 20-21 0.30401775230231465 1103 22-23 0.26826551047716407 1103 24-25 0.2428202689634702 1103 26-27 0.23648324763193074 1103 28-29 0.2379900466848781 1103 30-31 0.19309780306487312 1103 32-33 0.15110842940272562 1103 34-35 0.3021726768990405 1103 36-37 0.29622455317689145 1103 38-39 0.25965593421356203 1103 40-41 0.22962872249927813 1103 42-43 0.14409562747479754 1103 44-45 0.3261542762653562 1103 46-47 0.2951002491382724 1103 48-49 0.35499495420748417 1103 50-51 0.06801556650753326 1103 52-53 0.29241117729779376 1103 54-55 0.3169591607692297 1103 56-57 0.40856561620247334 1103 58-59 0.47175528261403343 1103 60-61 0.4647167991709935 1103 62-63 0.49391226092464535 1103 64-65 0.5502640224598778 1103 66-67 0.3938094207922376 1103 68-69 0.40930786824166887 1103 70-71 0.2676172738595213 1103 72-73 0.3576118006320712 1103 74-75 0.3443534133173145 1103 76-77 0.3960061052328214 1103 78-79 0.3135522302779279 1103 80-81 0.3829240967367227 1103 82-83 0.2840977705750234 1103 84-85 0.37164119448341637 1103 86-87 0.3727413110393556 1103 88-89 0.3295003744195597 1103 90-91 0.3935859381182709 1103 92-93 0.28808527845417586 1103 94-95 0.4200024847345887 1103 96-97 0.29034282806751577 1103 98-99 0.41267131671586554 1103 100-101 0.3175250372148639 1104 1 0.08893119319915854 1104 2 0.047699668556766994 1104 3 0.07659479583529816 1104 4 0.08254797585538398 1104 5 -0.027489440818236233 1104 6 0.018088232804061022 1104 7 3.368752297561173E-4 1104 8 0.10741210057075534 1104 9 0.19034656222809332 1104 10-11 0.07238905493415615 1104 12-13 0.03367721574250737 1104 14-15 0.0812141719718582 1104 16-17 0.12451791957162328 1104 18-19 0.0434049555382785 1104 20-21 0.17182938709659368 1104 22-23 0.1603099375979582 1104 24-25 0.14744621880615227 1104 26-27 0.20279053789635526 1104 28-29 0.16928660119878458 1104 30-31 0.10436746127554386 1104 32-33 0.08178682435779194 1104 34-35 0.13412369026548987 1104 36-37 0.09695683450043902 1104 38-39 0.1712490713699779 1104 40-41 0.14209317665031307 1104 42-43 0.1080896749264113 1104 44-45 0.19925140969272093 1104 46-47 0.28563296471388355 1104 48-49 0.2373383615994129 1104 50-51 0.08579954150790314 1104 52-53 0.20828588981675722 1104 54-55 0.2762215751946471 1104 56-57 0.25357298991509225 1104 58-59 0.1606381885935022 1104 60-61 0.22089805989502764 1104 62-63 0.2743939565704707 1104 64-65 0.3850982177340043 1104 66-67 0.28824210033644704 1104 68-69 0.2704684276526308 1104 70-71 0.26193998395675067 1104 72-73 0.28311319299797333 1104 74-75 0.32407001093495325 1104 76-77 0.3093793811837955 1104 78-79 0.25583434410233963 1104 80-81 0.2484700394534869 1104 82-83 0.23567036045596268 1104 84-85 0.14354567510214622 1104 86-87 0.2282694523681812 1104 88-89 0.3337069401715098 1104 90-91 0.3487087596583578 1104 92-93 0.28966382892024 1104 94-95 0.4185853388770582 1104 96-97 0.37402207625690664 1104 98-99 0.3346867713745567 1104 100-101 0.407490358664532 1105 1 0.014157945622493173 1105 2 -0.05060514154456541 1105 3 -0.009082926959600002 1105 4 0.07770089611082653 1105 5 0.09526266674322414 1105 6 0.12463590378764167 1105 7 0.05896011511990196 1105 8 0.05973862406035124 1105 9 0.2665956221871326 1105 10-11 0.17265725225758644 1105 12-13 0.15865426004596372 1105 14-15 0.21454646658588672 1105 16-17 0.1840384051936752 1105 18-19 0.24969772480548613 1105 20-21 0.22008039153815417 1105 22-23 0.2391647287945986 1105 24-25 0.1707199712003984 1105 26-27 0.23106967085657715 1105 28-29 0.31831435993337465 1105 30-31 0.3214410981642004 1105 32-33 0.3420591350096558 1105 34-35 0.3412121185169923 1105 36-37 0.3329324619907723 1105 38-39 0.4195416245835659 1105 40-41 0.2614013064985343 1105 42-43 0.3465594752014667 1105 44-45 0.31883750082580065 1105 46-47 0.3426767280354568 1105 48-49 0.533984129233751 1105 50-51 0.437624986806874 1105 52-53 0.44077827600428776 1105 54-55 0.461168039601894 1105 56-57 0.6489070628165692 1105 58-59 0.5296309632620506 1105 60-61 0.4919869701122792 1105 62-63 0.5352529358833351 1105 64-65 0.6003462015798391 1105 66-67 0.43701994320730364 1105 68-69 0.40556446763916654 1105 70-71 0.3490295340250995 1105 72-73 0.508487651305856 1105 74-75 0.42248243235584226 1105 76-77 0.5784542146770946 1105 78-79 0.44581527321597036 1105 80-81 0.5638125467556847 1105 82-83 0.42913868574959935 1105 84-85 0.45145739227133674 1105 86-87 0.5936780222178939 1105 88-89 0.5062643917360319 1105 90-91 0.44964713867456396 1105 92-93 0.4688732388861254 1105 94-95 0.42302087803469846 1105 96-97 0.37977180307373004 1105 98-99 0.38266438489619503 1105 100-101 0.38561098305947894 1106 1 0.19703769856594278 1106 2 0.19372520205784127 1106 3 0.1740863453816459 1106 4 0.154616155555658 1106 5 0.2236521116931769 1106 6 0.24753393387402411 1106 7 0.24675972830623039 1106 8 0.23149610528098208 1106 9 0.3174041100292868 1106 10-11 0.2123023054149158 1106 12-13 0.28719929705323466 1106 14-15 0.32594269850077495 1106 16-17 0.4375193343342971 1106 18-19 0.35495772110279233 1106 20-21 0.4023086273605685 1106 22-23 0.4381328974169989 1106 24-25 0.4051363434238695 1106 26-27 0.5400843595345961 1106 28-29 0.47730246009526667 1106 30-31 0.539025757969064 1106 32-33 0.541233118442058 1106 34-35 0.6768842565094246 1106 36-37 0.5329070551864774 1106 38-39 0.6394889202360545 1106 40-41 0.5367823100870055 1106 42-43 0.486132050033369 1106 44-45 0.5069368749124337 1106 46-47 0.37769419767531076 1106 48-49 0.3865577006426335 1106 50-51 0.401494935481189 1106 52-53 0.41990727560644103 1106 54-55 0.28548530559341145 1106 56-57 0.42754643981808726 1106 58-59 0.49337427181882276 1106 60-61 0.48924126268049406 1106 62-63 0.5073342061447761 1106 64-65 0.4928955085058462 1106 66-67 0.43180662452719787 1106 68-69 0.41823064586494496 1106 70-71 0.44638013610727967 1106 72-73 0.48983373924885143 1106 74-75 0.4435878055995204 1106 76-77 0.3561809162436518 1106 78-79 0.3794397015628732 1106 80-81 0.3296325168977674 1106 82-83 0.2144616278969309 1106 84-85 0.24731470450718263 1106 86-87 0.296089108250456 1106 88-89 0.373133918933128 1106 90-91 0.3385089138420696 1106 92-93 0.35260382434555737 1106 94-95 0.4182252944857012 1106 96-97 0.4504277319189214 1106 98-99 0.5032553195908562 1106 100-101 0.5569003094443445 1107 1 0.03421571750760677 1107 2 0.02617911943762863 1107 3 0.10179171339895277 1107 4 0.07673191793193723 1107 5 0.09690603233762118 1107 6 0.07344741537067279 1107 7 0.011980339100581716 1107 8 -0.0075629474724721035 1107 9 0.12232141970278576 1107 10-11 0.06622688228062401 1107 12-13 0.07418809595394293 1107 14-15 0.10043381140991414 1107 16-17 0.18406687083813011 1107 18-19 0.14321806532336723 1107 20-21 0.1856679109769388 1107 22-23 0.1836561489819033 1107 24-25 0.11847901811713513 1107 26-27 0.1286707238943805 1107 28-29 0.2149578974679045 1107 30-31 0.20484658267240263 1107 32-33 0.23012075835377033 1107 34-35 0.2166519986938269 1107 36-37 0.34337056545287936 1107 38-39 0.291926892752862 1107 40-41 0.23725594448814036 1107 42-43 0.3167227037022613 1107 44-45 0.36851520859634235 1107 46-47 0.1957055490228541 1107 48-49 0.19108009857491481 1107 50-51 0.16931317146293878 1107 52-53 0.18520263953922012 1107 54-55 0.22986117995471744 1107 56-57 0.25575983239004074 1107 58-59 0.2125391586930263 1107 60-61 0.3027036165919057 1107 62-63 0.2665957119141211 1107 64-65 0.19281025653955197 1107 66-67 0.185993078516546 1107 68-69 0.23482428446560988 1107 70-71 0.2107296780727239 1107 72-73 0.22385929035384322 1107 74-75 0.15249630219334165 1107 76-77 0.1666640886258719 1107 78-79 0.1388688917916241 1107 80-81 0.20111610515228762 1107 82-83 0.20884382752380048 1107 84-85 0.1369788866985715 1107 86-87 0.17372341441333816 1107 88-89 0.12546972264437528 1107 90-91 0.10654232014781684 1107 92-93 0.15592423632995178 1107 94-95 0.178854615745756 1107 96-97 0.25045696540432516 1107 98-99 0.20193543514474044 1107 100-101 0.13159446152301868 1108 1 0.10460821340289073 1108 2 0.1821794072693379 1108 3 0.14320661380987332 1108 4 0.1228922360413307 1108 5 0.16342802205732454 1108 6 0.13338560074398487 1108 7 0.1310257341738179 1108 8 0.19074825533886752 1108 9 0.20211307382870558 1108 10-11 0.09392393802517773 1108 12-13 0.11920054675097447 1108 14-15 0.1253656762480233 1108 16-17 0.199144305178244 1108 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-0.6798598586199986 2316 96-97 -0.6298822952107059 2316 98-99 -0.670946278765804 2316 100-101 -0.4531573300679952 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 10.0 7 304.0 8 922.0 9 860.0 10 913.0 11 1112.0 12 1523.0 13 1830.0 14 1951.0 15 2200.0 16 2555.0 17 3247.0 18 4309.0 19 5622.0 20 7441.0 21 9924.0 22 11562.0 23 11970.0 24 11065.0 25 9937.0 26 9417.0 27 9739.0 28 10929.0 29 12640.0 30 15452.0 31 18887.0 32 23661.0 33 32010.0 34 46703.0 35 75941.0 36 145474.0 37 343845.0 38 621725.0 39 247514.0 40 370.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78410274923469 19.71682912010989 13.832521799872621 23.6665463307828 2 14.934915896957262 21.277380082356707 40.61647192798631 23.17123209269972 3 16.96730092482529 26.380149862200735 32.079139921282724 24.57340929169125 4 11.782820144813963 18.27579223569397 40.22405954571736 29.717328073774702 5 12.417254404733603 38.53477853794836 36.515483706227826 12.532483351090216 6 29.310064482423627 39.42890350529625 18.506250128407192 12.754781883872937 7 25.169277368342268 33.841092062821204 23.941851352898187 17.04777921593834 8 24.469509528547487 35.753493409409096 21.717750716879017 18.059246345164407 9 25.630780158080263 15.259587981673725 21.401531494248825 37.70810036599719 10-11 22.99272408155838 26.566054127667567 31.020360244545998 19.420861546228057 12-13 24.21850648493013 25.077469894016374 29.812510822891998 20.8915127981615 14-15 21.644551279229145 26.475743514336113 27.674024765711902 24.205680440722837 16-17 21.03650286311353 29.07620196470343 28.32950313020049 21.557792041982548 18-19 20.84643086703472 28.446992043156555 30.16938009409679 20.537196995711934 20-21 21.734861892560602 27.770880477117103 30.04431882552177 20.44993880480052 22-23 21.44696145666465 27.791927859433436 29.620448436600235 21.140662247301684 24-25 21.25036284278554 27.817281618538576 29.83021158907383 21.102143949602052 26-27 21.32586663849046 28.058248437834777 29.608790976569722 21.00709394710504 28-29 21.27922914593808 28.02801771579012 29.727219096424264 20.965534041847537 30-31 21.51617343629389 27.80560178214509 29.78568472585431 20.89254005570671 32-33 21.239195451890595 27.98408044307085 29.65589807257134 21.120826032467207 34-35 21.53381291585587 28.157569567348474 29.444899372785894 20.86371814400977 36-37 21.523775142128418 27.934214426804964 29.423767217570212 21.118243213496406 38-39 21.39266772914447 28.04574524599883 29.530308500115932 21.031278524740767 40-41 21.592953600244194 27.919216466644947 29.488161590546884 20.999668342563975 42-43 21.37408904268402 28.17168702104117 29.31153199320249 21.14269194307232 44-45 21.56979628015368 28.258504958718923 29.118260823625754 21.053437937501652 46-47 21.546169356613927 28.165347374476468 28.89833965830479 21.39014361060482 48-49 21.559465004270457 28.304232594588406 28.901979085036377 21.234323316104756 50-51 21.607041703721315 28.45344909058357 28.68822146498666 21.251287740708456 52-53 21.577397985988206 28.49979308098018 28.607919275167077 21.314889657864537 54-55 21.54038736414519 28.414530704728026 28.69253594667653 21.352545984450256 56-57 21.534370569951836 28.466187084144135 28.623328138345176 21.376114207558857 58-59 21.54138527147482 28.365251692773686 28.714930161162034 21.378432874589464 60-61 21.56997238144714 28.40983467023565 28.72367652540408 21.296516422913125 62-63 21.36898210517356 28.462635708059274 28.839639227150123 21.32874295961704 64-65 21.398097519026276 28.308693827356162 28.749064461878472 21.54414419173909 66-67 21.46783363123802 28.449574862127363 28.606686566112828 21.47590494052179 68-69 21.425628021237817 28.3771385300825 28.68152961583503 21.51570383284465 70-71 21.3760555071277 28.290261891973596 28.63835544872077 21.69532715217793 72-73 21.468596736843033 28.34846336946345 28.561986187788552 21.62095370590497 74-75 21.412860677461676 28.380513804873896 28.508627495868954 21.69799802179547 76-77 21.313568898163556 28.395247613093716 28.58584791305292 21.705335575689805 78-79 21.346147637454397 28.35450951387238 28.514057285750766 21.78528556292246 80-81 21.343212615896665 28.439654489262225 28.395042161584676 21.822090733256434 82-83 21.36513722693293 28.388056810277273 28.512413673678434 21.734392289111362 84-85 21.435548394102955 28.353540956758327 28.472086477475177 21.73882417166354 86-87 21.30461708241247 28.334874219651145 28.482652555083014 21.877856142853368 88-89 21.44640797386657 28.268572082661947 28.417847279088264 21.86717266438322 90-91 21.43431568504871 28.222492244205533 28.479512082016246 21.86367998872952 92-93 21.433347127934653 28.247792130033194 28.43008631898401 21.888774423048137 94-95 21.414944542767667 28.123904870081272 28.550363502419923 21.910787084731137 96-97 21.295665266661384 28.075829216965598 28.583940149040394 22.044565367332623 98-99 21.320319447746343 28.117183670714063 28.431172276960375 22.13132460457922 100-101 21.61614027055029 27.936415692973267 28.443352616424967 22.00409142005148 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 267.0 1 191.5 2 182.5 3 263.5 4 231.0 5 239.0 6 366.5 7 713.5 8 1287.0 9 1613.5 10 1455.0 11 1304.0 12 1403.0 13 1522.5 14 1648.0 15 1789.0 16 1906.5 17 2085.0 18 2406.0 19 2767.5 20 3251.5 21 3961.0 22 4822.0 23 6026.0 24 7637.5 25 9660.0 26 12401.0 27 15731.0 28 19345.0 29 23053.5 30 26531.5 31 30353.5 32 34590.5 33 38768.5 34 42909.5 35 46692.0 36 50614.5 37 54200.5 38 56230.0 39 57939.5 40 60354.5 41 62401.0 42 63878.5 43 65400.5 44 66253.0 45 66538.0 46 66281.0 47 65525.5 48 64802.0 49 63227.5 50 60066.0 51 56061.0 52 52565.0 53 49203.0 54 44984.5 55 40254.0 56 34978.0 57 29783.5 58 25580.0 59 21654.0 60 17643.5 61 14241.5 62 11290.5 63 8450.5 64 6102.0 65 4364.5 66 3262.0 67 2493.5 68 1912.5 69 1485.5 70 1114.0 71 788.0 72 594.0 73 467.5 74 340.0 75 247.0 76 198.5 77 137.5 78 89.0 79 68.5 80 48.0 81 39.0 82 30.0 83 23.5 84 21.5 85 23.0 86 16.5 87 10.5 88 14.5 89 13.5 90 6.0 91 1.0 92 1.5 93 2.0 94 1.5 95 1.0 96 1.5 97 1.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.1740086230933366E-4 24-25 2.0545150904133393E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1703565.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.6809726093235 #Duplication Level Percentage of deduplicated Percentage of total 1 73.24208747243648 48.106115411267766 2 16.403217445605762 21.54758551499219 3 5.481996597371204 10.801886050690282 4 2.290094545354482 6.016625484247555 5 1.0561525278758277 3.4684562627340014 6 0.54484823838932 2.147169733313225 7 0.32995445639673215 1.5170210729042592 8 0.19374399497319622 1.0180235221644331 9 0.11503684000136429 0.6800158383473464 >10 0.3341617390013576 3.290541286155745 >50 0.005208898299213735 0.22984516470157634 >100 0.0031386247953764128 0.41590503406053086 >500 8.965487492230692E-5 0.03403622003989203 >1k 1.793097498446138E-4 0.4282204016091606 >5k 8.96548749223069E-5 0.29855300277206326 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5070 0.29761118595416086 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4473 0.2625670285548247 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2799 0.16430250680191247 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.9350215577333415E-5 0.0 20-21 0.0 0.0 0.0 1.1740086230933366E-4 0.0 22-23 0.0 0.0 0.0 1.4675107788666706E-4 0.0 24-25 0.0 0.0 0.0 7.924558205880021E-4 0.0 26-27 0.0 0.0 0.0 0.0013207597009800038 0.0 28-29 0.0 0.0 0.0 0.0038448782406306776 0.0 30-31 0.0 0.0 0.0 0.010125824374180028 0.0 32-33 0.0 0.0 0.0 0.021102805000102726 0.0 34-35 0.0 0.0 0.0 0.03897708628669877 0.0 36-37 0.0 0.0 0.0 0.06882625552884686 0.0 38-39 0.0 0.0 0.0 0.11484739355410564 0.0 40-41 0.0 0.0 0.0 0.16306979774766445 0.0 42-43 0.0 0.0 0.0 0.2061265639996126 0.0 44-45 0.0 0.0 0.0 0.26045381303325676 0.0 46-47 0.0 0.0 0.0 0.33388805240774494 0.0 48-49 0.0 0.0 0.0 0.41242922929268916 0.0 50-51 0.0 0.0 0.0 0.4970459008021414 0.0 52-53 0.0 0.0 0.0 0.5747946218664977 0.0 54-55 0.0 0.0 0.0 0.6637844754969725 0.0 56-57 0.0 0.0 0.0 0.7724096233486835 0.0 58-59 0.0 0.0 0.0 0.8985568499000625 0.0 60-61 0.0 0.0 0.0 1.015077205742076 0.0 62-63 0.0 0.0 0.0 1.1268428266605617 0.0 64-65 0.0 0.0 0.0 1.2689565704860102 0.0 66-67 0.0 0.0 0.0 1.4525715191378081 0.0 68-69 0.0 0.0 0.0 1.6626310120247834 0.0 70-71 0.0 0.0 0.0 1.8980197409549973 0.0 72-73 0.0 0.0 0.0 2.1300625453093955 0.0 74-75 0.0 0.0 0.0 2.3764869552966865 0.0 76-77 0.0 0.0 0.0 2.6414607015288527 0.0 78-79 0.0 0.0 0.0 2.943357018957304 0.0 80-81 0.0 0.0 0.0 3.2748090034721304 0.0 82-83 0.0 0.0 0.0 3.6305629664849888 0.0 84-85 0.0 0.0 0.0 3.988518195666147 0.0 86-87 0.0 0.0 0.0 4.3786412611200625 0.0 88-89 0.0 0.0 0.0 4.815225717832897 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2155 0.0 63.259865 1 GTATCAA 4255 0.0 41.08108 1 TCAACGC 4995 0.0 33.473473 4 CAACGCA 5125 0.0 32.99512 5 AACGCAG 5210 0.0 32.8215 6 ATCAACG 5120 0.0 32.749023 3 TATCAAC 5350 0.0 31.341122 2 ACGCAGA 5895 0.0 28.84648 7 CGCAGAG 5870 0.0 28.564735 8 GCAGAGT 6840 0.0 24.444445 9 GTACATG 3500 0.0 23.071428 1 AGAGTAC 6205 0.0 22.812248 10-11 TACATGG 3480 0.0 22.658045 2 CAGAGTA 6710 0.0 21.944859 10-11 ACATGGG 3530 0.0 21.933428 3 GAGTACT 5030 0.0 21.341948 12-13 AGTACTT 5475 0.0 20.171232 12-13 CATGGGG 2065 0.0 20.012108 4 TATGCCG 125 0.0060323123 19.0 5 GTACTTT 5615 0.0 18.864649 14-15 >>END_MODULE