FastQCFastQC Report
Fri 10 Feb 2017
SRR2031788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1703565
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60790.3568399209892197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54660.3208565566914089No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34270.20116637756704323No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT29140.17105305638469914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22800.062.7885631
GTATCAA44450.040.6593781
ATCAACG53800.033.1082883
TCAACGC54000.032.985664
CAACGCA54050.032.955155
TATCAAC55350.032.266952
AACGCAG55700.032.2347536
ACGCAGA61150.028.973447
CGCAGAG61900.028.4689188
GCAGAGT71300.024.0494639
GTACATG32100.022.8173181
TACATGG32800.022.1567122
AGAGTAC65300.021.93117310-11
GAGTACT54550.021.76872412-13
CAGAGTA69500.021.56265610-11
ACATGGG33050.020.2644753
GTACTTT61150.019.14732714-15
AGTACTT59550.018.94390712-13
GTATACG1650.001451717617.2948271
CATGGGG19050.016.7058094
Produced by FastQC (version 0.11.5)