FastQCFastQC Report
Fri 10 Feb 2017
SRR2031787_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031787_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081632
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43710.4041115647466051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38890.3595492736901275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23200.21449069554155203No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17300.063.150291
GTATCAA33400.042.238021
TCAACGC39950.034.59954
CAACGCA40850.033.8372085
ATCAACG40900.033.7958453
AACGCAG42000.033.023816
TATCAAC42350.032.8630452
ACGCAGA47400.029.061187
CGCAGAG47450.028.7302448
ATATCGC700.00839135627.1428558
TACATGG24300.026.388892
GCGTGCG2251.0186341E-1025.3333349
GTACATG26600.025.178571
GCAGAGT52750.024.9431279
ACATGGG24750.023.7979813
GAGTACT40400.022.63304312-13
AGAGTAC49100.021.91191310-11
CAGAGTA51650.021.84172210-11
GGACCGT1100.002877257321.590916
CATGGGG15150.021.00664
Produced by FastQC (version 0.11.5)