##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031787_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1081632 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.62779762433064 34.0 31.0 34.0 31.0 34.0 2 32.76124966716961 34.0 33.0 34.0 31.0 34.0 3 32.82905831188426 34.0 33.0 34.0 31.0 34.0 4 36.01800612407917 37.0 37.0 37.0 35.0 37.0 5 36.04729889648235 37.0 37.0 37.0 35.0 37.0 6 36.11767495784149 37.0 37.0 37.0 35.0 37.0 7 36.14153704772048 37.0 37.0 37.0 35.0 37.0 8 36.1383464986243 37.0 37.0 37.0 35.0 37.0 9 37.98044344102245 39.0 39.0 39.0 37.0 39.0 10-11 38.0219205792728 39.0 39.0 39.0 37.0 39.0 12-13 37.996081384426496 39.0 39.0 39.0 37.0 39.0 14-15 39.581324794384784 41.0 40.0 41.0 37.5 41.0 16-17 39.518801218898844 41.0 40.0 41.0 37.0 41.0 18-19 39.53323126534718 41.0 40.0 41.0 37.0 41.0 20-21 39.476563655661074 41.0 40.0 41.0 37.0 41.0 22-23 39.3887172346972 41.0 40.0 41.0 37.0 41.0 24-25 39.35430072335138 41.0 40.0 41.0 37.0 41.0 26-27 39.23225366853052 41.0 40.0 41.0 36.0 41.0 28-29 39.107718706547146 41.0 39.0 41.0 36.0 41.0 30-31 38.94584387296234 41.0 39.0 41.0 35.5 41.0 32-33 38.80480838214846 41.0 39.0 41.0 35.0 41.0 34-35 38.6047149122807 40.5 38.5 41.0 35.0 41.0 36-37 38.475334494541585 40.0 38.0 41.0 35.0 41.0 38-39 38.28037169758291 40.0 38.0 41.0 34.0 41.0 40-41 38.1848230266856 40.0 38.0 41.0 34.0 41.0 42-43 38.01646585899825 40.0 38.0 41.0 33.5 41.0 44-45 38.138541574213775 40.0 38.0 41.0 34.0 41.0 46-47 37.9833672635425 40.0 38.0 41.0 33.0 41.0 48-49 37.7917193648117 40.0 38.0 41.0 33.0 41.0 50-51 36.73397560353244 39.0 36.5 40.0 31.5 40.5 52-53 36.75323446421704 39.5 37.0 40.0 31.5 40.5 54-55 37.5215928337919 40.0 38.0 41.0 32.5 41.0 56-57 37.512546318895886 40.0 37.5 41.0 32.5 41.0 58-59 37.394132662495196 40.0 37.0 41.0 32.0 41.0 60-61 37.203479094553416 40.0 36.5 41.0 32.0 41.0 62-63 36.948867544599274 40.0 36.0 41.0 31.5 41.0 64-65 36.63812553622674 39.0 35.5 41.0 31.0 41.0 66-67 36.375167801988106 39.0 35.0 41.0 31.0 41.0 68-69 36.052247437206 38.5 35.0 41.0 30.5 41.0 70-71 35.690102548741166 37.0 35.0 40.0 30.0 41.0 72-73 35.2850008135854 37.0 35.0 39.5 29.0 41.0 74-75 34.91700227064288 36.5 35.0 39.0 29.0 41.0 76-77 34.538437749622794 36.0 35.0 39.0 29.0 40.5 78-79 34.1626625321736 35.5 35.0 37.0 29.0 39.5 80-81 33.80146621031922 35.0 35.0 37.0 28.0 39.0 82-83 33.509853628590875 35.0 34.0 36.5 27.5 39.0 84-85 33.190403945149555 35.0 34.0 36.0 27.0 37.0 86-87 32.92461437901245 35.0 34.0 36.0 26.5 37.0 88-89 32.712836713410844 35.0 34.0 35.5 26.0 37.0 90-91 32.536960352504366 35.0 34.0 35.0 25.5 36.0 92-93 32.395878172983046 35.0 34.0 35.0 25.0 36.0 94-95 32.234337094316736 35.0 34.0 35.0 25.0 36.0 96-97 32.12980292742819 35.0 34.0 35.0 25.0 36.0 98-99 32.016464472204966 35.0 34.0 35.0 24.0 35.5 100-101 30.541325515517293 34.0 30.5 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2914472056015853 1101 2 0.2856968023097508 1101 3 0.333081339475811 1101 4 0.28185621339724776 1101 5 0.22211750455218038 1101 6 0.31477382469911674 1101 7 0.29517275311803814 1101 8 0.37402405700814967 1101 9 0.33514436848761875 1101 10-11 0.32355133108627854 1101 12-13 0.29739326187957715 1101 14-15 0.22578306042986895 1101 16-17 0.3227117685454104 1101 18-19 0.4249676801563993 1101 20-21 0.42874183550916456 1101 22-23 0.3894717442917468 1101 24-25 0.40090313321670834 1101 26-27 0.3109668812352169 1101 28-29 0.42634465105757613 1101 30-31 0.38601502025901624 1101 32-33 0.13718436884357743 1101 34-35 0.4764266742183878 1101 36-37 0.27867709143498587 1101 38-39 0.47722729490331517 1101 40-41 0.4804391498390217 1101 42-43 0.35832060700162316 1101 44-45 0.5678295555142867 1101 46-47 0.6980589173068239 1101 48-49 0.6299969411188542 1101 50-51 0.5435838082986848 1101 52-53 0.3718493295433305 1101 54-55 0.490843547402271 1101 56-57 0.49235207024843675 1101 58-59 0.4423035456877926 1101 60-61 0.5646138160303238 1101 62-63 0.5001196448426839 1101 64-65 0.5877882387707771 1101 66-67 0.48934401907537506 1101 68-69 0.5989366342183331 1101 70-71 0.4468827889783782 1101 72-73 0.4345847813498196 1101 74-75 0.3668321846964986 1101 76-77 0.41492391280114305 1101 78-79 0.4369871089002615 1101 80-81 0.45149442951828433 1101 82-83 0.30094764349473735 1101 84-85 0.3265952208879952 1101 86-87 0.4061409467549808 1101 88-89 0.4773605807500232 1101 90-91 0.6338279857990301 1101 92-93 0.3816510079061075 1101 94-95 0.25917422117264977 1101 96-97 0.18778615981557323 1101 98-99 0.21904909014951102 1101 100-101 0.377394911649354 1102 1 0.07079659457664178 1102 2 0.10002933054602181 1102 3 0.14772124502480466 1102 4 0.05386327150056047 1102 5 -0.0500683211095847 1102 6 0.06811317696895003 1102 7 0.07084004482810968 1102 8 0.17361790740413596 1102 9 0.22723178196540061 1102 10-11 0.2172142140539819 1102 12-13 0.3320548767551017 1102 14-15 0.28689841566286844 1102 16-17 0.33906064050925266 1102 18-19 0.35375067177361075 1102 20-21 0.41482818924257714 1102 22-23 0.43176184674228324 1102 24-25 0.4020824846634383 1102 26-27 0.2700786251063221 1102 28-29 0.3419747231047623 1102 30-31 0.3752285201677026 1102 32-33 0.35142249752021115 1102 34-35 0.3190070488843304 1102 36-37 0.32179865594692814 1102 38-39 0.34787031795275425 1102 40-41 0.15409318445562548 1102 42-43 0.09815079068207666 1102 44-45 0.235339259120245 1102 46-47 0.13899874594718398 1102 48-49 0.223512308698389 1102 50-51 0.24341828754493378 1102 52-53 0.1610335064280477 1102 54-55 0.11223507922161247 1102 56-57 0.14606773465929024 1102 58-59 0.1314537695973712 1102 60-61 0.20727161376833436 1102 62-63 0.233329641421669 1102 64-65 0.2670850263563338 1102 66-67 0.2727015310425145 1102 68-69 0.18804390246998537 1102 70-71 0.1635387810354345 1102 72-73 0.3379692756434096 1102 74-75 0.30522554020215864 1102 76-77 0.35024928261848487 1102 78-79 0.1485713752973723 1102 80-81 0.1530638543497389 1102 82-83 0.25327072148626684 1102 84-85 0.19925870317715066 1102 86-87 0.3714349615142396 1102 88-89 0.44707298361749537 1102 90-91 0.40475150672256177 1102 92-93 0.28329091088290426 1102 94-95 0.344536373379718 1102 96-97 0.37117659587980256 1102 98-99 0.21556440885733963 1102 100-101 0.24524067254324322 1103 1 0.18509503090169943 1103 2 0.15638682893872868 1103 3 0.17483281958163133 1103 4 0.22223832630756135 1103 5 0.16962653168960884 1103 6 0.18590335873508934 1103 7 0.17173149629920914 1103 8 0.18226094288272776 1103 9 0.21049662074763376 1103 10-11 0.30762134203088465 1103 12-13 0.26521873435004295 1103 14-15 0.38868949705894096 1103 16-17 0.3539124855457274 1103 18-19 0.3669957913948565 1103 20-21 0.4152808439459861 1103 22-23 0.4462371421321336 1103 24-25 0.34846817600404734 1103 26-27 0.404271718402029 1103 28-29 0.3649385720668974 1103 30-31 0.5175483840418806 1103 32-33 0.49960131487033976 1103 34-35 0.4683819651119734 1103 36-37 0.5002706935859891 1103 38-39 0.47876987972393437 1103 40-41 0.4267287593668456 1103 42-43 0.5653199779027958 1103 44-45 0.73657454642251 1103 46-47 0.7916007437617196 1103 48-49 0.8152967110238052 1103 50-51 0.649565417929928 1103 52-53 0.7623861318253304 1103 54-55 0.9323244288300643 1103 56-57 0.8794546823012865 1103 58-59 0.7996618476379993 1103 60-61 0.7875647204676213 1103 62-63 0.7967052755358139 1103 64-65 0.7977866013902499 1103 66-67 0.71035161135039 1103 68-69 0.7480158231117215 1103 70-71 0.6321653232987288 1103 72-73 0.6572254452645367 1103 74-75 0.6753232954437536 1103 76-77 0.6292010092945617 1103 78-79 0.6372947640851621 1103 80-81 0.7483127406033532 1103 82-83 0.696379351799699 1103 84-85 0.6432215275607547 1103 86-87 0.795303211166086 1103 88-89 0.6465795502344136 1103 90-91 0.46068361192473617 1103 92-93 0.6075319617233532 1103 94-95 0.6273995592055499 1103 96-97 0.5650860331340226 1103 98-99 0.5719433756914327 1103 100-101 0.5637675966948983 1104 1 0.317657359084464 1104 2 0.2867593760995959 1104 3 0.3356000329035922 1104 4 0.18396824278200086 1104 5 0.2200841843370398 1104 6 0.27001454357620247 1104 7 0.2504003538001811 1104 8 0.27757908783590324 1104 9 0.3538509144668893 1104 10-11 0.27646357036216074 1104 12-13 0.3365904283494743 1104 14-15 0.34735593200274195 1104 16-17 0.4657788025215339 1104 18-19 0.4327861243384774 1104 20-21 0.4640953708627009 1104 22-23 0.46100526127981567 1104 24-25 0.4618765032809904 1104 26-27 0.5481832002697189 1104 28-29 0.5940345369292785 1104 30-31 0.5499858978662573 1104 32-33 0.44511431768261644 1104 34-35 0.5651647038655128 1104 36-37 0.568956508987128 1104 38-39 0.5885417380359357 1104 40-41 0.6128870050141515 1104 42-43 0.68955502914514 1104 44-45 0.8075317724892344 1104 46-47 0.7383055393716234 1104 48-49 0.7105295398332743 1104 50-51 0.5103226250374959 1104 52-53 0.675155114667298 1104 54-55 0.7063230174272022 1104 56-57 0.811734203266937 1104 58-59 0.6705011057035364 1104 60-61 0.5697889439327142 1104 62-63 0.7301012793697765 1104 64-65 0.792248425049145 1104 66-67 0.6942305466891767 1104 68-69 0.5811811573719439 1104 70-71 0.6747786305105805 1104 72-73 0.6298884675626013 1104 74-75 0.7067902064727107 1104 76-77 0.6975160681205708 1104 78-79 0.6332812188307386 1104 80-81 0.6805643494972884 1104 82-83 0.634910650185013 1104 84-85 0.6575672791327776 1104 86-87 0.7005001229323398 1104 88-89 0.7345952652574326 1104 90-91 0.8163676683387138 1104 92-93 0.7999377716474143 1104 94-95 0.7063406910663872 1104 96-97 0.6411706541091533 1104 98-99 0.5698820955279658 1104 100-101 0.4035591514499579 1105 1 0.07918975147510565 1105 2 0.06786746762234941 1105 3 0.14772124502480466 1105 4 0.1355983334670583 1105 5 0.09659763124585652 1105 6 0.1883317562039224 1105 7 0.16743278028294384 1105 8 0.11884809419652242 1105 9 0.3061453145915678 1105 10-11 0.2241608907210022 1105 12-13 0.2155507158922063 1105 14-15 0.21939600486248878 1105 16-17 0.2892195747569062 1105 18-19 0.3232227957780438 1105 20-21 0.2837431504911976 1105 22-23 0.36871495221033257 1105 24-25 0.26655978742425646 1105 26-27 0.314196272165141 1105 28-29 0.3227508222511588 1105 30-31 0.2174371704766429 1105 32-33 0.3100817353501313 1105 34-35 0.4666730369553278 1105 36-37 0.500983626838746 1105 38-39 0.5058045298888985 1105 40-41 0.366618631793024 1105 42-43 0.5486133072005188 1105 44-45 0.5595383376587577 1105 46-47 0.4848860990669408 1105 48-49 0.5772302986266027 1105 50-51 0.5274909329965496 1105 52-53 0.6055136664337581 1105 54-55 0.6783713698034219 1105 56-57 0.6694078539492665 1105 58-59 0.6383468729224973 1105 60-61 0.5112824712989621 1105 62-63 0.6342278877876097 1105 64-65 0.7474503965481318 1105 66-67 0.6009364680045479 1105 68-69 0.5767720713331528 1105 70-71 0.6951291949787901 1105 72-73 0.7310904585427949 1105 74-75 0.6565773741962246 1105 76-77 0.5795163993012338 1105 78-79 0.5766848793510846 1105 80-81 0.6748057022728773 1105 82-83 0.6124799789597475 1105 84-85 0.46441103004596584 1105 86-87 0.6094166751468677 1105 88-89 0.5534160672990609 1105 90-91 0.5959815506526951 1105 92-93 0.6147045327979725 1105 94-95 0.7068886002449588 1105 96-97 0.7795929678930875 1105 98-99 0.5746754485373273 1105 100-101 0.5868243005299583 1106 1 0.2000286000108389 1106 2 0.12141198584726709 1106 3 0.3055159218308887 1106 4 0.2263617597002323 1106 5 0.23318093127409156 1106 6 0.2921868368511511 1106 7 0.25219395521355636 1106 8 0.2146705346231741 1106 9 0.326240206628043 1106 10-11 0.1464629942589255 1106 12-13 0.18724718604044455 1106 14-15 0.2501337097805205 1106 16-17 0.36650085191278237 1106 18-19 0.34741146519956345 1106 20-21 0.37676292088766417 1106 22-23 0.3581775545547643 1106 24-25 0.33404111517944557 1106 26-27 0.4265313202528205 1106 28-29 0.4613406781897922 1106 30-31 0.480798779534652 1106 32-33 0.5178271878289493 1106 34-35 0.5681393169161737 1106 36-37 0.5915224728078528 1106 38-39 0.6394375785705577 1106 40-41 0.6352743768311768 1106 42-43 0.67081247047863 1106 44-45 0.5747332549948894 1106 46-47 0.6021646629265689 1106 48-49 0.5751270357045257 1106 50-51 0.5188134265891406 1106 52-53 0.6510970434609433 1106 54-55 0.7372443985386923 1106 56-57 0.6350346987157209 1106 58-59 0.6859665971195952 1106 60-61 0.6580076327217483 1106 62-63 0.6680799251430969 1106 64-65 0.6837672252181406 1106 66-67 0.6828995561756699 1106 68-69 0.781286776298856 1106 70-71 0.7548473788302701 1106 72-73 0.9607512781782575 1106 74-75 0.8406813997745459 1106 76-77 0.7783414320257762 1106 78-79 0.8274846600874142 1106 80-81 0.7937379101993685 1106 82-83 0.7999325421080457 1106 84-85 0.7897109592802636 1106 86-87 0.8822184468559868 1106 88-89 0.6835630802898791 1106 90-91 0.644981755983224 1106 92-93 0.7339057787142806 1106 94-95 0.8198474314742654 1106 96-97 0.825039763453546 1106 98-99 0.846066947853501 1106 100-101 0.8462589632562327 1107 1 0.1501818729618165 1107 2 0.2057555535966884 1107 3 0.2318163090101777 1107 4 0.20455251327486934 1107 5 0.17539598608394158 1107 6 0.24223584248854024 1107 7 0.2448014026802099 1107 8 0.19294296426011215 1107 9 0.22345001331644454 1107 10-11 0.2857649941059961 1107 12-13 0.2549966108324426 1107 14-15 0.21471323446059642 1107 16-17 0.27252819775356585 1107 18-19 0.26837510371173323 1107 20-21 0.3220025966858273 1107 22-23 0.19053917445175017 1107 24-25 0.30126120957611136 1107 26-27 0.24104324495792184 1107 28-29 0.30915558285471434 1107 30-31 0.3520938143251797 1107 32-33 0.31299144360362163 1107 34-35 0.37967267202978405 1107 36-37 0.45348645192657955 1107 38-39 0.5202574324573064 1107 40-41 0.4610228423638816 1107 42-43 0.49468586215531474 1107 44-45 0.5327039697820837 1107 46-47 0.5868356300406532 1107 48-49 0.573932306069679 1107 50-51 0.5502613172613877 1107 52-53 0.5173272934939561 1107 54-55 0.6175403091746503 1107 56-57 0.5674790373481571 1107 58-59 0.4683876493853205 1107 60-61 0.6754949989989214 1107 62-63 0.567342060120609 1107 64-65 0.6184011774359064 1107 66-67 0.6013529591132283 1107 68-69 0.6220810151822178 1107 70-71 0.544790448877059 1107 72-73 0.6603336875873467 1107 74-75 0.5885465265288445 1107 76-77 0.5069596815861175 1107 78-79 0.7112344254859622 1107 80-81 0.624114106666795 1107 82-83 0.6584218130014818 1107 84-85 0.6279567312407011 1107 86-87 0.6607442460649438 1107 88-89 0.6988234705276852 1107 90-91 0.5911216062553635 1107 92-93 0.3818150452979907 1107 94-95 0.43473523984484785 1107 96-97 0.47664791298677756 1107 98-99 0.5357491758374593 1107 100-101 0.6126158980752585 1108 1 0.055806709733815296 1108 2 0.07848015532037778 1108 3 0.10932838788194488 1108 4 0.0856637694775273 1108 5 0.11284212084497369 1108 6 0.11667760273925865 1108 7 0.10338556303538837 1108 8 0.05348096373778333 1108 9 0.15981231417828923 1108 10-11 0.1603650855136749 1108 12-13 0.12948815562219806 1108 14-15 0.167990852637665 1108 16-17 0.22022505071778653 1108 18-19 0.14331552549288062 1108 20-21 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-0.46456791767987937 2316 90-91 -0.36697035810152556 2316 92-93 -0.7519766549632294 2316 94-95 -0.8077440447838953 2316 96-97 -0.7818447031806812 2316 98-99 -0.9075787170293452 2316 100-101 -0.7299283323949233 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 12.0 7 359.0 8 784.0 9 659.0 10 672.0 11 874.0 12 1095.0 13 1202.0 14 1346.0 15 1410.0 16 1675.0 17 2299.0 18 3085.0 19 4086.0 20 5733.0 21 7541.0 22 8733.0 23 8891.0 24 7927.0 25 6768.0 26 6426.0 27 6698.0 28 7212.0 29 8352.0 30 9895.0 31 12132.0 32 15448.0 33 20614.0 34 30264.0 35 49864.0 36 96701.0 37 222552.0 38 383114.0 39 146972.0 40 236.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.86929380787551 19.63523638353895 13.915453684802227 23.58001612378332 2 15.161903493979468 21.435941244341883 40.1124411999645 23.28971406171415 3 16.995891393745747 26.405746131771252 32.15520620691695 24.44315626756605 4 11.921799650897903 18.553075352800214 39.6536899795864 29.87143501671548 5 12.586535901304696 38.25228913345759 36.09240481050857 13.068770154729151 6 29.16019496464602 38.96038578740274 18.603184817017247 13.276234430933995 7 25.02976982929499 34.054465844205794 23.87429365995089 17.041470666548328 8 24.734845122925357 35.073204195142154 21.814628265435935 18.377322416496554 9 26.030110055915507 15.045690216265792 21.453784651341675 37.470415076477025 10-11 23.140263971480135 26.44517728765421 30.66209209786693 19.75246664299873 12-13 24.37044207271974 25.10766138575782 29.71389529895565 20.80800124256679 14-15 21.801222596964585 26.47850655306056 27.726620514185974 23.99365033578888 16-17 20.921533386586198 28.90114197804799 28.475535117304222 21.701789518061595 18-19 20.741388938197094 28.10271885447176 30.504691059436112 20.651201147895033 20-21 21.70701310612112 27.493454335670542 30.40179099730777 20.397741560900563 22-23 21.427224256701223 27.50799487070914 30.039865721304217 21.02491515128542 24-25 21.263186117248967 27.495862725701027 30.18495234044914 21.05599881660087 26-27 21.32693929173693 27.61068459513032 30.044183234223837 21.01819287890891 28-29 21.212528845300437 27.690610115085352 30.111627614567617 20.985233425046594 30-31 21.45739031389604 27.50635151326884 30.093737981124818 20.942520191710305 32-33 21.175085426466673 27.694816721398773 30.022271900239637 21.107825951894917 34-35 21.53509696458685 27.798086595071155 29.80736516671104 20.85945127363096 36-37 21.60341964734771 27.739471465341264 29.59980843762019 21.05730044969084 38-39 21.412643117067542 27.82859604745422 29.756423626519922 21.002337208958316 40-41 21.52219978698855 27.723569568947664 29.7144037898287 21.039826854235084 42-43 21.418652554658145 27.854159270435787 29.519975370551165 21.2072128043549 44-45 21.611000784000474 28.019973521493448 29.220243114109053 21.14878258039703 46-47 21.58053755806041 27.980912177154522 29.05960622466791 21.378944040117158 48-49 21.586593222094024 28.05750939321322 28.968262773290732 21.387634611402028 50-51 21.59366586787373 28.230396290050592 28.74045886216384 21.43547897991184 52-53 21.65912251116831 28.347903908168398 28.55772573296648 21.435247847696814 54-55 21.624545131800836 28.344575604272066 28.55846535605455 21.472413907872546 56-57 21.567917739120144 28.38497751545812 28.51764740688145 21.529457338540283 58-59 21.52543563799888 28.29543689535813 28.622766338274015 21.556361128368984 60-61 21.617195127363097 28.041237685275583 28.723817342682167 21.617749844679153 62-63 21.474078059820716 28.189855699535514 28.756083399899413 21.579982840744357 64-65 21.524511109138782 28.11367452146386 28.738563578000655 21.623250791396703 66-67 21.419761989290258 28.194986834709034 28.575892725067302 21.809358450933406 68-69 21.532230925120558 28.20413967042395 28.51075966687376 21.75286973758173 70-71 21.534126209283748 27.924423463802846 28.598451229253573 21.942999097659833 72-73 21.533386586195675 28.041422591047606 28.486583237182334 21.938607585574392 74-75 21.516745066714 28.104660365077955 28.431388864234787 21.947205703973253 76-77 21.435386527025827 28.066616002485134 28.453069066003966 22.044928404485077 78-79 21.41509311854679 28.073781101150853 28.435595470548208 22.07553030975415 80-81 21.333133635099554 28.12925283275643 28.304127466643 22.233486065501022 82-83 21.438160113606106 28.071793364101655 28.371664299872783 22.118382222419456 84-85 21.424107274932695 27.94018668086743 28.37110958255673 22.264596461643148 86-87 21.389714801337238 27.941018756841512 28.385670912103194 22.283595529718056 88-89 21.451750687849472 27.823788497381734 28.325299177539126 22.399161637229668 90-91 21.471997869885506 27.791429987278484 28.342587867222864 22.393984275613146 92-93 21.446896911333983 27.677158220171 28.3946850684891 22.48125980000592 94-95 21.393690275435638 27.61327327593858 28.452514348687906 22.54052209993787 96-97 21.371362903464394 27.534503417058666 28.498463433034527 22.595670246442413 98-99 21.438714830922162 27.45929299429011 28.390802047276708 22.71119012751102 100-101 21.70123480074554 27.23814569095589 28.437629434040414 22.62299007425816 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 404.0 1 300.0 2 249.0 3 367.0 4 346.0 5 295.5 6 410.5 7 731.5 8 1228.5 9 1535.5 10 1368.5 11 1126.0 12 1138.0 13 1239.5 14 1340.5 15 1418.0 16 1516.0 17 1658.5 18 1844.0 19 2066.0 20 2316.0 21 2767.0 22 3356.5 23 4113.5 24 5167.0 25 6373.5 26 8001.5 27 9941.5 28 11971.0 29 14188.0 30 16458.5 31 18638.5 32 21089.0 33 23465.5 34 25439.5 35 27285.0 36 29368.5 37 31389.5 38 32870.5 39 34564.0 40 36230.5 41 37557.0 42 38751.0 43 39735.5 44 40491.5 45 41104.5 46 41612.5 47 41656.5 48 41406.0 49 40783.5 50 39236.0 51 37022.0 52 34591.0 53 32355.5 54 29831.0 55 26693.5 56 23696.5 57 20689.0 58 17992.0 59 15623.0 60 13065.5 61 10574.5 62 8278.5 63 6166.5 64 4396.5 65 3244.5 66 2439.0 67 1813.0 68 1345.5 69 991.5 70 756.0 71 564.5 72 416.0 73 316.5 74 254.5 75 196.5 76 142.0 77 110.5 78 78.0 79 54.5 80 51.5 81 40.5 82 26.0 83 21.5 84 16.5 85 12.0 86 14.5 87 13.5 88 7.5 89 4.0 90 3.0 91 4.0 92 2.5 93 2.0 94 2.5 95 2.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.245288600928966E-5 24-25 1.8490577201857932E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1081632.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.61749588972744 #Duplication Level Percentage of deduplicated Percentage of total 1 79.80984484792333 58.754009250517804 2 13.945682052348845 20.532923823364737 3 3.6991224236846096 8.169603894636012 4 1.2160428802430905 3.5808812695211203 5 0.5598522298897333 2.0607459616381085 6 0.26649159829323754 1.1771066485199586 7 0.15259485904336575 0.786355598589296 8 0.08556935011138582 0.5039521024089275 9 0.05508625017048231 0.36497806159553814 >10 0.20088340485210154 2.271871584652388 >50 0.004797909991070161 0.23675167544668102 >100 0.003528169934519249 0.532827517245752 >500 1.260058785789015E-4 0.04656671098678615 >1k 3.7801763573670446E-4 0.9814259008768873 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4371 0.4041115647466051 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3889 0.3595492736901275 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2320 0.21449069554155203 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 9.245288600928966E-5 0.0 12-13 0.0 0.0 0.0 9.245288600928966E-5 0.0 14-15 0.0 0.0 0.0 9.245288600928966E-5 0.0 16-17 0.0 0.0 0.0 9.245288600928966E-5 0.0 18-19 0.0 0.0 0.0 9.245288600928966E-5 0.0 20-21 0.0 0.0 0.0 1.3867932901393448E-4 0.0 22-23 0.0 0.0 0.0 3.2358510103251383E-4 0.0 24-25 0.0 0.0 0.0 9.245288600928966E-4 0.0 26-27 0.0 0.0 0.0 0.0024037750362415314 0.0 28-29 0.0 0.0 0.0 0.0060556640336084724 0.0 30-31 0.0 0.0 0.0 0.014099065116416672 0.0 32-33 0.0 0.0 0.0 0.02972360285198663 0.0 34-35 0.0 0.0 0.0 0.0541311647584391 0.0 36-37 0.0 0.0 0.0 0.09296137688234075 0.0 38-39 0.0 0.0 0.0 0.15259348835833259 0.0 40-41 0.0 0.0 0.0 0.2196218307150676 0.0 42-43 0.0 0.0 0.0 0.2798548859501198 0.0 44-45 0.0 0.0 0.0 0.3508124759622496 0.0 46-47 0.0 0.0 0.0 0.44423611727463685 0.0 48-49 0.0 0.0 0.0 0.5445012721517115 0.0 50-51 0.0 0.0 0.0 0.6461994467619301 0.0 52-53 0.0 0.0 0.0 0.745401393449898 0.0 54-55 0.0 0.0 0.0 0.8536637229667761 0.0 56-57 0.0 0.0 0.0 0.9998779621904677 0.0 58-59 0.0 0.0 0.0 1.1549214520280464 0.0 60-61 0.0 0.0 0.0 1.3083932428034672 0.0 62-63 0.0 0.0 0.0 1.4504933285997454 0.0 64-65 0.0 0.0 0.0 1.6283264548386143 0.0 66-67 0.0 0.0 0.0 1.8536341380432533 0.0 68-69 0.0 0.0 0.0 2.121747507470193 0.0 70-71 0.0 0.0 0.0 2.4098307002751396 0.0 72-73 0.0 0.0 0.0 2.7049865388597967 0.0 74-75 0.0 0.0 0.0 3.0177084257862195 0.0 76-77 0.0 0.0 0.0 3.3464708884352534 0.0 78-79 0.0 0.0 0.0 3.734264518801219 0.0 80-81 0.0 0.0 0.0 4.154925150143487 0.0 82-83 0.0 0.0 0.0 4.598190512115027 0.0 84-85 0.0 0.0 0.0 5.0542143723558475 0.0 86-87 0.0 0.0 0.0 5.544538253306115 0.0 88-89 0.0 0.0 0.0 6.095372548149463 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1730 0.0 63.15029 1 GTATCAA 3340 0.0 42.23802 1 TCAACGC 3995 0.0 34.5995 4 CAACGCA 4085 0.0 33.837208 5 ATCAACG 4090 0.0 33.795845 3 AACGCAG 4200 0.0 33.02381 6 TATCAAC 4235 0.0 32.863045 2 ACGCAGA 4740 0.0 29.06118 7 CGCAGAG 4745 0.0 28.730244 8 ATATCGC 70 0.008391356 27.142855 8 TACATGG 2430 0.0 26.38889 2 GCGTGCG 225 1.0186341E-10 25.333334 9 GTACATG 2660 0.0 25.17857 1 GCAGAGT 5275 0.0 24.943127 9 ACATGGG 2475 0.0 23.797981 3 GAGTACT 4040 0.0 22.633043 12-13 AGAGTAC 4910 0.0 21.911913 10-11 CAGAGTA 5165 0.0 21.841722 10-11 GGACCGT 110 0.0028772573 21.59091 6 CATGGGG 1515 0.0 21.0066 4 >>END_MODULE