FastQCFastQC Report
Fri 10 Feb 2017
SRR2031787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081632
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49790.4603229194402533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45980.42509836987071387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27670.2558171355877045No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT26600.2459246767847105No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20100.064.848911
GTATCAA35600.042.4937741
TCAACGC45650.032.9840244
ATCAACG45950.032.6653023
TATCAAC46550.032.142232
CAACGCA46600.032.1077425
AACGCAG47300.031.7329966
ACGCAGA53050.028.293517
CGCAGAG52900.028.0145768
GCAGAGT59900.024.264979
GAGTACT44500.023.85627612-13
CAGAGTA56900.023.16519210-11
AGAGTAC53300.022.3682310-11
GTACTTT50150.021.12122314-15
AGTACTT46750.020.7268712-13
GTACATG23250.020.6655331
GTACTAA3052.0372681E-1020.276441
TACATGG22750.019.2084182
GCACCGT1507.7216665E-418.999636
GTGGTAT7150.018.6294561
Produced by FastQC (version 0.11.5)