FastQCFastQC Report
Fri 10 Feb 2017
SRR2031786_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031786_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130697
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106930.3415533346088746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87760.28032096367039033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56650.1809501206919737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49500.056.8080181
GTATCAA92250.044.127321
ATCAACG114200.035.0634463
TCAACGC114150.035.037194
CAACGCA115800.034.743055
TATCAAC118500.033.9113542
AACGCAG119200.033.7520566
ACGCAGA133350.029.9568427
CGCAGAG134400.029.6874668
GTACATG82750.026.4622041
GCAGAGT153500.025.4983399
TACATGG81300.025.3566762
ACATGGG83000.023.864433
AGAGTAC136300.022.84388410-11
GAGTACT105400.022.69091212-13
CATGGGG51450.021.7881164
CAGAGTA151750.021.56669610-11
GTACTTT116000.020.3103214-15
AGTACTT114000.020.06247512-13
GTGTAGC20650.020.0120831
Produced by FastQC (version 0.11.5)