FastQCFastQC Report
Fri 10 Feb 2017
SRR2031786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130697
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127200.4062993001239021No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107450.3432143065905132No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67280.21490422100893186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51650.060.691581
GTATCAA97200.043.8485371
TCAACGC119050.035.389624
ATCAACG119500.035.176863
CAACGCA121200.034.6442685
TATCAAC124500.033.916742
AACGCAG125250.033.827426
ACGCAGA137550.030.6298437
CGCAGAG138700.030.375888
GCAGAGT155000.026.6912049
GTACATG75150.025.6986581
TACATGG73900.024.9384232
ACATGGG73150.024.1551823
GAGTACT121050.023.48448612-13
AGAGTAC144300.023.17330710-11
CAGAGTA154250.023.00261110-11
AGTACTT129650.021.04743812-13
GTACTTT134300.021.0083614-15
CATGGGG41850.018.8405844
GTATAGG11250.018.1814821
Produced by FastQC (version 0.11.5)