FastQCFastQC Report
Fri 10 Feb 2017
SRR2031785_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031785_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527921
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21970.4161607513245353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20230.3832012744331065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10970.20779624224078982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13300.067.142861
GTATCAA21050.042.4228022
ATCAACG27400.032.76464
TCAACGC27300.032.7106255
TATCAAC27500.032.472733
CAACGCA28200.031.8351046
AACGCAG28400.031.6109147
GTATTAT2201.8189894E-1228.0681821
GTGGTAT4250.027.9411751
ACGCAGA32350.027.4574998
CGCAGAG32450.026.4946089
TGGTATC4700.025.2659592
GGACCGT950.001225675925.0000026
GTACTAC1751.4829675E-724.4285721
GAGTACT21000.023.7512-13
GAGGCGT1201.7091092E-423.756
CAGAGTA33450.023.14648810-11
TAGATAC1252.2536593E-422.8000015
GTGTAGC2303.616151E-922.7173921
GTACTTT21400.022.64018614-15
Produced by FastQC (version 0.11.5)