##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031785_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 527921 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.561201391874924 34.0 31.0 34.0 31.0 34.0 2 32.68418191358177 34.0 31.0 34.0 31.0 34.0 3 32.749847041508104 34.0 33.0 34.0 31.0 34.0 4 35.942906230288244 37.0 37.0 37.0 35.0 37.0 5 35.96887981345694 37.0 37.0 37.0 35.0 37.0 6 36.05176153250202 37.0 37.0 37.0 35.0 37.0 7 36.07669518734811 37.0 37.0 37.0 35.0 37.0 8 36.07465889782752 37.0 37.0 37.0 35.0 37.0 9 37.909933493837144 39.0 39.0 39.0 37.0 39.0 10-11 37.947630421976015 39.0 39.0 39.0 37.0 39.0 12-13 37.9153263461768 39.0 39.0 39.0 37.0 39.0 14-15 39.4764870122613 41.0 40.0 41.0 37.0 41.0 16-17 39.40178738864338 41.0 40.0 41.0 37.0 41.0 18-19 39.41946522301632 41.0 40.0 41.0 37.0 41.0 20-21 39.366914746713995 41.0 40.0 41.0 37.0 41.0 22-23 39.27467840832246 41.0 40.0 41.0 36.0 41.0 24-25 39.22626491463685 41.0 40.0 41.0 36.0 41.0 26-27 39.097432191558966 41.0 39.0 41.0 36.0 41.0 28-29 38.97209336245385 41.0 39.0 41.0 35.0 41.0 30-31 38.792704779692414 41.0 39.0 41.0 35.0 41.0 32-33 38.6263153009636 40.5 38.0 41.0 35.0 41.0 34-35 38.414351768541124 40.0 38.0 41.0 35.0 41.0 36-37 38.28043400433019 40.0 38.0 41.0 34.0 41.0 38-39 38.0813663407972 40.0 38.0 41.0 34.0 41.0 40-41 37.95440321563264 40.0 38.0 41.0 33.0 41.0 42-43 37.75045035147304 40.0 38.0 41.0 33.0 41.0 44-45 37.87687078180258 40.0 38.0 41.0 33.0 41.0 46-47 37.686511807637885 40.0 38.0 41.0 33.0 41.0 48-49 37.44709340980942 40.0 38.0 41.0 32.0 41.0 50-51 36.35038670558663 39.0 36.0 40.0 30.0 40.5 52-53 36.34987052987094 39.0 36.5 40.0 30.5 40.5 54-55 37.06959374603397 40.0 37.0 41.0 31.0 41.0 56-57 37.025646829733994 40.0 36.0 41.0 31.0 41.0 58-59 36.86798592971297 40.0 36.0 41.0 31.0 41.0 60-61 36.644404181686276 39.5 35.0 41.0 31.0 41.0 62-63 36.34808238353845 39.0 35.0 41.0 30.5 41.0 64-65 36.00048965659634 39.0 35.0 41.0 29.5 41.0 66-67 35.698528757143585 37.5 35.0 41.0 29.0 41.0 68-69 35.35522739197721 37.0 35.0 40.0 29.0 41.0 70-71 34.98481875129044 36.5 35.0 39.0 29.0 41.0 72-73 34.59315977201134 36.0 35.0 39.0 28.0 41.0 74-75 34.242315611616135 35.5 35.0 38.5 28.0 40.5 76-77 33.892277443026515 35.0 34.5 37.0 27.0 39.5 78-79 33.550164702673314 35.0 34.0 37.0 27.0 39.0 80-81 33.228075791643064 35.0 34.0 36.5 26.0 39.0 82-83 32.97926867845757 35.0 34.0 36.0 26.0 37.0 84-85 32.714717732387996 35.0 34.0 36.0 25.5 37.0 86-87 32.48598559254131 35.0 34.0 35.0 25.0 36.5 88-89 32.317380820236366 35.0 34.0 35.0 25.0 36.0 90-91 32.16685356331724 35.0 34.0 35.0 24.0 36.0 92-93 32.044381640434835 35.0 34.0 35.0 24.0 36.0 94-95 31.89380513372266 35.0 33.5 35.0 23.5 35.5 96-97 31.80307659668776 35.0 33.0 35.0 23.0 35.0 98-99 31.693063924337164 35.0 33.0 35.0 21.5 35.0 100-101 30.169103900015344 34.0 30.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20379399020117006 1101 2 0.23750741774616557 1101 3 0.1603898004644364 1101 4 0.210965030016375 1101 5 0.2626002811818964 1101 6 0.20369762005531555 1101 7 0.18049062904141522 1101 8 0.29132074204971303 1101 9 0.22871050101953472 1101 10-11 0.30977822837346025 1101 12-13 0.24816641258875194 1101 14-15 0.2832800441706098 1101 16-17 0.3581491518336861 1101 18-19 0.4617165186997383 1101 20-21 0.2978793189169622 1101 22-23 0.3688681386590744 1101 24-25 0.3549723752925189 1101 26-27 0.3602447148476813 1101 28-29 0.18492505484717014 1101 30-31 0.34815512726166986 1101 32-33 0.5564631612890949 1101 34-35 0.45235848070365847 1101 36-37 0.329521533383037 1101 38-39 0.22349877565098097 1101 40-41 0.47211125250913 1101 42-43 0.2166880370300177 1101 44-45 0.4048823971766211 1101 46-47 0.5022299002640622 1101 48-49 0.4011763459799127 1101 50-51 0.5045044037555044 1101 52-53 0.5821117038902344 1101 54-55 0.5921368214746607 1101 56-57 0.7044775650651971 1101 58-59 0.6317509314027845 1101 60-61 0.5567029671766477 1101 62-63 0.4306358529168861 1101 64-65 0.4078922657381199 1101 66-67 0.3982940282812706 1101 68-69 0.3361163589562821 1101 70-71 0.39821185153090255 1101 72-73 0.45590832497217804 1101 74-75 0.16194800792246866 1101 76-77 0.29534936163406655 1101 78-79 0.3632061507274642 1101 80-81 0.36336152919749054 1101 82-83 0.36505497580024127 1101 84-85 0.5871533107288869 1101 86-87 0.42806344834744436 1101 88-89 0.5346041979417109 1101 90-91 0.5322529406973686 1101 92-93 0.6729950157848137 1101 94-95 0.48749765504257425 1101 96-97 0.2576744400641431 1101 98-99 0.38045265050413235 1101 100-101 0.5703245983340786 1102 1 0.18198131213848967 1102 2 0.11010357159231887 1102 3 0.09584207966671698 1102 4 0.10759964540098821 1102 5 0.03850840084001561 1102 6 0.1869597283174187 1102 7 0.08656256636334803 1102 8 0.16801233749131228 1102 9 0.05029169760072705 1102 10-11 0.11177253036776591 1102 12-13 0.22880209634944038 1102 14-15 0.28977933191989536 1102 16-17 0.3600633256228605 1102 18-19 0.23606658992481044 1102 20-21 0.32307518786283396 1102 22-23 0.4002962013371345 1102 24-25 0.2806757228708676 1102 26-27 0.3930972789502363 1102 28-29 0.4350140861862073 1102 30-31 0.24875163723318394 1102 32-33 0.28830076812670313 1102 34-35 0.45111204195721655 1102 36-37 0.4678762342377283 1102 38-39 0.40730397508118443 1102 40-41 0.2609289163267903 1102 42-43 0.33817129913828836 1102 44-45 0.2466291920484167 1102 46-47 0.38404079769996 1102 48-49 0.43790177333033853 1102 50-51 0.5987885917896989 1102 52-53 0.5412908349443697 1102 54-55 0.314180981018815 1102 56-57 0.5366980066606359 1102 58-59 0.4426483673002295 1102 60-61 0.5270555312792027 1102 62-63 0.6181803685864011 1102 64-65 0.6650592885301378 1102 66-67 0.6324464499336955 1102 68-69 0.7661377264776448 1102 70-71 0.6833347147787663 1102 72-73 0.5201444360832923 1102 74-75 0.4188479366974036 1102 76-77 0.29615064368535116 1102 78-79 0.29580942708074076 1102 80-81 0.3833045491405045 1102 82-83 0.26142249716776433 1102 84-85 0.2854706184211935 1102 86-87 0.18879172470071381 1102 88-89 0.3555176594801708 1102 90-91 0.4414409748854027 1102 92-93 0.3587589046736994 1102 94-95 0.49969494848986074 1102 96-97 0.4740205939102964 1102 98-99 0.4341830636095452 1102 100-101 0.6387006667101467 1103 1 0.27576797210162596 1103 2 0.2953411281534031 1103 3 0.2368886692427168 1103 4 0.2441946680254219 1103 5 0.2757507336705842 1103 6 0.13158223543992875 1103 7 0.10936506343055186 1103 8 0.11668838910853907 1103 9 0.3146833516982639 1103 10-11 0.24487438221961355 1103 12-13 0.10344073385572727 1103 14-15 0.2709780079715145 1103 16-17 0.2893567310192111 1103 18-19 0.2584501159848074 1103 20-21 0.3411485496862028 1103 22-23 0.35741451874957164 1103 24-25 0.1447772395459168 1103 26-27 0.05707729403319206 1103 28-29 -0.03028987727952881 1103 30-31 0.030069720021856483 1103 32-33 -0.023287969932617614 1103 34-35 -0.02190622517869656 1103 36-37 0.03215162388077175 1103 38-39 0.22484210144283878 1103 40-41 0.0900411167625208 1103 42-43 0.2057293266227802 1103 44-45 0.18874834740287127 1103 46-47 0.1569951717572664 1103 48-49 0.30028550887584515 1103 50-51 0.20402363452473793 1103 52-53 0.24380570841511684 1103 54-55 0.20497618799049633 1103 56-57 -0.12492005936919526 1103 58-59 -0.08077734914019885 1103 60-61 0.31801518437121246 1103 62-63 0.23401537780376458 1103 64-65 0.2894674919824567 1103 66-67 0.2735768337111324 1103 68-69 0.15879735443139253 1103 70-71 0.4108673945173251 1103 72-73 0.18363739737036866 1103 74-75 0.08113635633876015 1103 76-77 0.18505524398700857 1103 78-79 0.10670162584057863 1103 80-81 0.13329931200291867 1103 82-83 -0.11458303324952368 1103 84-85 0.01864369534427368 1103 86-87 0.11110926282707823 1103 88-89 0.15458440156162112 1103 90-91 0.29766583662496515 1103 92-93 -0.03903835525591148 1103 94-95 0.09595353739551626 1103 96-97 0.07978982467952989 1103 98-99 0.1593695057077511 1103 100-101 0.25909722276846736 1104 1 0.2328485112615084 1104 2 0.16229701768111227 1104 3 0.048283157107796626 1104 4 0.1423512310246693 1104 5 0.2592918163908777 1104 6 0.16126185814280802 1104 7 0.0762511185519017 1104 8 0.20816189508273197 1104 9 0.30701591653425453 1104 10-11 0.17669256403780054 1104 12-13 0.2555888593280642 1104 14-15 0.1913189529362569 1104 16-17 0.28324622046272907 1104 18-19 0.3573423318400799 1104 20-21 0.34887338299243 1104 22-23 0.404397289740551 1104 24-25 0.3624405611764061 1104 26-27 0.4424938610503091 1104 28-29 0.2920461314449909 1104 30-31 0.45330687957912374 1104 32-33 0.31502146345204807 1104 34-35 0.4020555859240176 1104 36-37 0.35969412242189946 1104 38-39 0.21986759578433634 1104 40-41 0.23358669567061696 1104 42-43 0.410451758773398 1104 44-45 0.5530950130491306 1104 46-47 0.752852460843016 1104 48-49 0.6603368802131229 1104 50-51 0.441297988078162 1104 52-53 0.5561903142333833 1104 54-55 0.4483227866722501 1104 56-57 0.5316572894106173 1104 58-59 0.13334163720860914 1104 60-61 0.216647673632977 1104 62-63 0.13671356880564645 1104 64-65 0.17605832932569143 1104 66-67 -0.02060538625767805 1104 68-69 -0.02978663340019949 1104 70-71 0.19397894780671265 1104 72-73 0.1330026695810531 1104 74-75 0.20890940408445147 1104 76-77 0.23869380788548966 1104 78-79 0.33439048964435614 1104 80-81 0.05199484355754436 1104 82-83 0.24218946921380535 1104 84-85 0.35662548100047076 1104 86-87 0.2783770155941525 1104 88-89 0.3681365812560671 1104 90-91 0.43002595256921694 1104 92-93 0.5706231870321687 1104 94-95 0.5783203497896778 1104 96-97 0.6635026235086059 1104 98-99 0.5852039312001835 1104 100-101 0.7803130110841181 1105 1 -0.07574110099628939 1105 2 -0.1374479747994357 1105 3 0.14556525645174645 1105 4 -0.031189014392829506 1105 5 -0.10940110660557423 1105 6 -0.14113782800178853 1105 7 -0.023092836462154764 1105 8 0.12217423134392646 1105 9 0.19722755097988198 1105 10-11 0.19693623788971593 1105 12-13 0.20896389474577148 1105 14-15 0.35154431855601587 1105 16-17 0.2430792668217947 1105 18-19 0.29139782321995966 1105 20-21 0.4554229350946031 1105 22-23 0.4085340783894438 1105 24-25 0.3878802856810992 1105 26-27 0.3482304404622738 1105 28-29 0.32321807625874754 1105 30-31 0.3732170820594547 1105 32-33 0.30061462837870323 1105 34-35 0.3117458716632129 1105 36-37 0.36400309563521915 1105 38-39 0.3455819437715206 1105 40-41 0.21542013434893903 1105 42-43 0.15640106240670804 1105 44-45 0.2743783329418932 1105 46-47 0.4455313475281386 1105 48-49 0.48825257516310216 1105 50-51 0.3903836662455973 1105 52-53 0.41255133513528364 1105 54-55 0.44866933535253395 1105 56-57 0.3065815499977873 1105 58-59 0.28815953568510366 1105 60-61 0.31324539382216443 1105 62-63 0.33963416774638233 1105 64-65 0.40049981926706124 1105 66-67 0.4348424025873783 1105 68-69 0.4067448284249551 1105 70-71 0.5815162726252723 1105 72-73 0.36509002600310936 1105 74-75 0.27137752814451943 1105 76-77 0.16975459557538386 1105 78-79 0.1604404885545847 1105 80-81 0.41193904704046247 1105 82-83 0.2636275372594028 1105 84-85 0.30099896893666056 1105 86-87 0.24749495112301645 1105 88-89 0.15880374195440083 1105 90-91 0.10977574006295043 1105 92-93 -0.01460163369971923 1105 94-95 0.14246890801640433 1105 96-97 0.1691236866938013 1105 98-99 0.3900011239379211 1105 100-101 0.40043463005493507 1106 1 0.0986943678796024 1106 2 0.11523884213340096 1106 3 0.2842095553588422 1106 4 0.21143231073165225 1106 5 0.17091209712147304 1106 6 0.05751101607812359 1106 7 0.12088584254534851 1106 8 0.21011506145776337 1106 9 0.33299599508458755 1106 10-11 0.17122708763043448 1106 12-13 0.2662867879548756 1106 14-15 0.2573580077901525 1106 16-17 0.2943508822175289 1106 18-19 0.17070902293898627 1106 20-21 0.3264267984915037 1106 22-23 0.3903967728505364 1106 24-25 0.29410304046902525 1106 26-27 0.2828977131519821 1106 28-29 0.6278975382755689 1106 30-31 0.5638726199393389 1106 32-33 0.4944352593313326 1106 34-35 0.29420563655381926 1106 36-37 0.38429935826356854 1106 38-39 0.5358759338134576 1106 40-41 0.4045819631611991 1106 42-43 0.049967576878948705 1106 44-45 0.3178549769515655 1106 46-47 0.4129527883479156 1106 48-49 0.12760757382021382 1106 50-51 0.09927567707137541 1106 52-53 0.0745123706082822 1106 54-55 0.25015478046957895 1106 56-57 -0.1118305318979651 1106 58-59 0.18047819361952833 1106 60-61 0.28546818992984413 1106 62-63 0.2904925856130447 1106 64-65 0.2676092959523899 1106 66-67 0.35267394195477664 1106 68-69 0.18892775097107517 1106 70-71 0.07554970030537334 1106 72-73 0.12308643118460338 1106 74-75 0.37536281831248886 1106 76-77 0.23914079064593352 1106 78-79 0.24496004312764796 1106 80-81 0.2421913041319712 1106 82-83 0.45300009681150044 1106 84-85 0.40414546812059626 1106 86-87 0.14069785176967287 1106 88-89 0.18248409234590213 1106 90-91 0.24058787980634833 1106 92-93 0.26189155887099247 1106 94-95 0.04941475848021071 1106 96-97 0.2753732993211173 1106 98-99 0.14022941557272972 1106 100-101 0.09088976713321628 1107 1 0.14385653687779865 1107 2 0.2723608531457202 1107 3 0.20291687290655602 1107 4 0.14412877161458226 1107 5 0.2925806769996768 1107 6 0.33344930041378973 1107 7 0.1876368202288674 1107 8 0.3053190616912431 1107 9 -0.004373890317282303 1107 10-11 0.1560394307633075 1107 12-13 0.23782987412722179 1107 14-15 0.23934333767322613 1107 16-17 0.3377444655006059 1107 18-19 0.33706892721355075 1107 20-21 0.36026729501853794 1107 22-23 0.2889064134910413 1107 24-25 0.4171666434030499 1107 26-27 0.3429556931897224 1107 28-29 0.3035303573116934 1107 30-31 0.39708856639536094 1107 32-33 0.38306883716661133 1107 34-35 0.3922483238702057 1107 36-37 0.3967125341298541 1107 38-39 0.4221008107518003 1107 40-41 0.37613477752779545 1107 42-43 0.40753708482688467 1107 44-45 0.5878104218218851 1107 46-47 0.6660998591886269 1107 48-49 0.6057483213346586 1107 50-51 0.3346435000960639 1107 52-53 0.504584164681873 1107 54-55 0.6179116534388172 1107 56-57 0.6504610415401757 1107 58-59 0.4378142249054022 1107 60-61 0.6253362756157799 1107 62-63 0.6706120478384676 1107 64-65 0.52555236655963 1107 66-67 0.6000210633820942 1107 68-69 0.7338292043632961 1107 70-71 0.7461044019416505 1107 72-73 0.9717760434187781 1107 74-75 0.5220931722241886 1107 76-77 0.4978698993487711 1107 78-79 0.5105455831397094 1107 80-81 0.5325876307658177 1107 82-83 0.5070265964126435 1107 84-85 0.5198759582270185 1107 86-87 0.7469744827014395 1107 88-89 0.7092191157908516 1107 90-91 0.7191041356189487 1107 92-93 0.6683530254935519 1107 94-95 0.6285964227796867 1107 96-97 0.5854283609005932 1107 98-99 0.8519869111463976 1107 100-101 0.8029468910898672 1108 1 0.12454794419110726 1108 2 0.07143534729325296 1108 3 0.05066208658952576 1108 4 0.16659497250379474 1108 5 0.20405876428758063 1108 6 0.17665772070233032 1108 7 0.18789088065895498 1108 8 0.18360596850550337 1108 9 0.05883271525389944 1108 10-11 0.07244447567756396 1108 12-13 0.1116616498281573 1108 14-15 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-0.9750586640929129 2316 100-101 -0.9396313030576557 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 160.0 8 450.0 9 387.0 10 382.0 11 475.0 12 615.0 13 678.0 14 742.0 15 767.0 16 938.0 17 1183.0 18 1589.0 19 2112.0 20 2985.0 21 3976.0 22 4778.0 23 4919.0 24 4382.0 25 3812.0 26 3483.0 27 3522.0 28 3959.0 29 4405.0 30 5403.0 31 6591.0 32 8263.0 33 11350.0 34 17058.0 35 28969.0 36 57468.0 37 124116.0 38 169361.0 39 48593.0 40 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.84080004394597 18.092858590584576 13.605065909482668 23.461275455986787 2 16.813689927091364 20.648544005637206 37.995078809140004 24.542687258131423 3 18.619452531723496 25.132927085681377 30.673149959937184 25.574470422657935 4 13.485919294742962 17.909687244871865 36.447119928928764 32.15727353145641 5 14.037516976971935 36.95818124302689 34.1892063395849 14.815095440416274 6 31.132120146764386 35.99894681211772 17.59164723509768 15.277285806020219 7 26.663080271480013 33.3405945207711 21.703057843881947 18.293267363866942 8 26.313975007624247 33.02350162240184 20.569176069904398 20.093347300069517 9 27.01786820376534 14.95640446203125 19.902220218555428 38.12350711564799 10-11 24.92730919967192 25.443106070794684 28.118695789710962 21.510888939822436 12-13 26.265577614832523 24.275412419661276 27.351156707158836 22.107853258347365 14-15 23.655338582856146 25.29109469030404 25.87157927038326 25.18198745645655 16-17 22.39994241562658 27.75500501021933 26.74860443134484 23.096448142809244 18-19 22.301916385216728 26.465796965833903 29.05027456759629 22.18201208135308 20-21 23.645772757666393 25.6671926292002 28.866156110478652 21.820878502654754 22-23 23.353611007718015 25.74137583215655 28.5520262994144 22.352986860711034 24-25 22.919803985081046 26.038274811097917 28.613480477118646 22.42844072670239 26-27 22.605844435057517 26.25601178964277 28.723426421756287 22.414717353543427 28-29 22.534242812845104 26.089320182375776 28.917868393187618 22.458568611591506 30-31 22.844895353660867 25.95255729550444 28.865208999073726 22.337338351760962 32-33 22.642781779849635 26.18166354435607 28.644721464006924 22.53083321178737 34-35 22.91270853025358 26.322120165706615 28.470737098922 22.29443420511781 36-37 22.980900551408258 26.257621879031145 28.504927820639832 22.25654974892077 38-39 22.79081529243959 26.249287298667795 28.588557757694804 22.37133965119781 40-41 22.97067174823506 26.254496411394886 28.5104210667884 22.264410773581652 42-43 22.797255649993087 26.321551898863653 28.242577961475295 22.63861448966796 44-45 22.846505443049246 26.584754158292622 27.934387910312335 22.634352488345794 46-47 22.829173304339097 26.587500781366906 27.81372591732475 22.769599996969244 48-49 22.899070126022643 26.790277333161587 27.607160919910367 22.7034916209054 50-51 22.96858810314422 26.945887736990954 27.46556776487391 22.61995639499092 52-53 23.04123154790205 26.975816457386614 27.223201956353314 22.75975003835801 54-55 22.977869794912497 27.05821514961519 27.10879089863824 22.855124156834073 56-57 22.88666296661811 27.073274220953515 27.175941097247502 22.86412171518087 58-59 22.745827500705598 26.95725307385006 27.50354693221145 22.793372493232887 60-61 22.843001130851018 26.961704497453216 27.521352626624058 22.673941745071705 62-63 22.66863792120412 27.060867061548983 27.490287372542483 22.78020764470442 64-65 22.685496504211805 26.94494062558602 27.461305763551742 22.908257106650424 66-67 22.67119512199742 27.053006036888092 27.324637587820906 22.95116125329358 68-69 22.644581291518996 27.144402287463464 27.27917624038445 22.93184018063309 70-71 22.559057131654168 26.914822482909372 27.35617639760494 23.169943987831513 72-73 22.655378361535153 26.99797886426189 27.277944995558045 23.06869777864491 74-75 22.681613347451606 27.09894094002701 27.11740961242307 23.10203610009831 76-77 22.65263173846087 27.122618725150165 27.095815472390754 23.128934063998212 78-79 22.620524661833873 27.189674212618936 27.05660506022681 23.13319606532038 80-81 22.55773117568727 27.232862492683562 26.977331835634498 23.232074495994663 82-83 22.544471616018306 27.23267307040258 27.016636958938932 23.206218354640182 84-85 22.62876453105673 27.156430602306024 26.913117682380506 23.301687184256735 86-87 22.42267308934481 27.148096021942674 26.996463486014004 23.43276740269851 88-89 22.516437118432492 27.0811352456144 27.097993828622087 23.30443380733102 90-91 22.642118801866186 27.028286429219524 27.052248347764152 23.27734642115013 92-93 22.611527103487074 26.94503533672652 27.110306276886124 23.333131282900283 94-95 22.499104979722347 26.985476993716862 27.083408312986222 23.43200971357457 96-97 22.583871450463235 26.87797984925775 27.084923691234103 23.453225009044914 98-99 22.53774712504333 26.941815157949765 27.008491800856564 23.511945916150335 100-101 22.721676159879983 26.89985812271154 27.051585369780707 23.32688034762777 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 52.0 1 44.5 2 45.0 3 58.5 4 59.0 5 69.0 6 119.0 7 228.0 8 399.0 9 577.0 10 576.0 11 510.0 12 544.5 13 598.5 14 641.0 15 675.0 16 735.0 17 805.0 18 882.0 19 918.5 20 999.0 21 1143.0 22 1298.0 23 1481.0 24 1742.5 25 2115.0 26 2568.0 27 3111.0 28 3703.0 29 4419.5 30 5158.0 31 6018.5 32 7061.5 33 7992.0 34 8899.5 35 9802.0 36 10933.5 37 12009.0 38 12861.5 39 13748.0 40 14657.0 41 15524.5 42 16291.5 43 17312.5 44 18376.5 45 19220.0 46 19796.0 47 20343.5 48 21049.0 49 21429.5 50 21360.0 51 20650.0 52 19868.0 53 19579.0 54 18643.5 55 17247.0 56 16015.0 57 14299.0 58 12975.5 59 11790.0 60 9934.0 61 8161.0 62 6547.5 63 5045.0 64 3728.5 65 2806.5 66 2227.0 67 1770.5 68 1383.5 69 1048.0 70 769.5 71 598.5 72 484.5 73 372.5 74 280.5 75 213.5 76 154.0 77 97.0 78 64.0 79 48.0 80 37.5 81 27.5 82 25.0 83 20.5 84 15.5 85 11.0 86 9.5 87 10.5 88 5.5 89 5.0 90 4.5 91 2.0 92 2.5 93 2.0 94 2.5 95 2.0 96 0.5 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.471114049261159E-5 24-25 3.7884456197044634E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 527921.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.43337704205163 #Duplication Level Percentage of deduplicated Percentage of total 1 82.08425991870587 62.73977187584212 2 12.975754949918969 19.835615409848483 3 2.9351137900400173 6.730219769263615 4 0.926283916623199 2.8319603138899714 5 0.3965322432517084 1.515414922889418 6 0.22036735969544452 1.0106052894817994 7 0.13377528283177142 0.7157427642111517 8 0.08148765478490703 0.49827013139578735 9 0.053573998254047135 0.36853574473816203 >10 0.18388266920594867 2.123586190979447 >50 0.005231463134002499 0.26619136419306816 >100 0.0029894028432962735 0.3516844297015415 >500 0.0 0.0 >1k 7.473507108194709E-4 1.0124017935654286 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2197 0.4161607513245353 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2023 0.3832012744331065 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1097 0.20779624224078982 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 1.8942228098522317E-4 0.0 0.0 0.0 9 0.0 1.8942228098522317E-4 0.0 0.0 0.0 10-11 0.0 1.8942228098522317E-4 0.0 0.0 0.0 12-13 0.0 1.8942228098522317E-4 0.0 0.0 0.0 14-15 0.0 2.8413342147783473E-4 0.0 0.0 0.0 16-17 0.0 3.7884456197044634E-4 0.0 0.0 0.0 18-19 0.0 3.7884456197044634E-4 0.0 9.471114049261159E-5 0.0 20-21 0.0 3.7884456197044634E-4 0.0 3.7884456197044634E-4 0.0 22-23 0.0 3.7884456197044634E-4 0.0 5.682668429556695E-4 0.0 24-25 0.0 3.7884456197044634E-4 0.0 0.0018942228098522316 0.0 26-27 0.0 3.7884456197044634E-4 0.0 0.0042620013221675215 0.0 28-29 0.0 3.7884456197044634E-4 0.0 0.012786003966502563 0.0 30-31 0.0 3.7884456197044634E-4 0.0 0.025477296792512517 0.0 32-33 0.0 3.7884456197044634E-4 0.0 0.047355570246305795 0.0 34-35 0.0 3.7884456197044634E-4 0.0 0.08637656012926176 0.0 36-37 0.0 3.7884456197044634E-4 0.0 0.14007777678857253 0.0 38-39 0.0 5.682668429556695E-4 0.0 0.20410250776157796 0.0 40-41 0.0 7.576891239408927E-4 0.0 0.2681272387345834 0.0 42-43 0.0 9.471114049261158E-4 0.0 0.3297841911952736 0.0 44-45 0.0 9.471114049261158E-4 0.0 0.39958630173832826 9.471114049261159E-5 46-47 0.0 0.0010418225454187273 0.0 0.49382388652847686 1.8942228098522317E-4 48-49 0.0 0.001136533685911339 0.0 0.5868302264922214 1.8942228098522317E-4 50-51 0.0 0.001136533685911339 0.0 0.6789841661915325 1.8942228098522317E-4 52-53 0.0 0.001136533685911339 0.0 0.7665919711471982 1.8942228098522317E-4 54-55 0.0 0.001136533685911339 0.0 0.8743732490277902 3.7884456197044634E-4 56-57 0.0 0.001136533685911339 0.0 1.0081053794033576 3.7884456197044634E-4 58-59 0.0 0.001136533685911339 0.0 1.1489408453158712 3.7884456197044634E-4 60-61 0.0 0.001136533685911339 0.0 1.2693187048819805 3.7884456197044634E-4 62-63 0.0 0.001136533685911339 0.0 1.3822143843491734 3.7884456197044634E-4 64-65 0.0 0.001136533685911339 0.0 1.5382036327405046 3.7884456197044634E-4 66-67 0.0 0.0013259559668965621 0.0 1.740222495411245 3.7884456197044634E-4 68-69 0.0 0.0013259559668965621 0.0 1.97756861348573 3.7884456197044634E-4 70-71 0.0 0.0013259559668965621 0.0 2.2184190437584412 3.7884456197044634E-4 72-73 0.0 0.0013259559668965621 0.0 2.468172321237458 3.7884456197044634E-4 74-75 0.0 0.0013259559668965621 0.0 2.7287226687326323 3.7884456197044634E-4 76-77 0.0 0.0013259559668965621 0.0 3.0071734217809105 3.7884456197044634E-4 78-79 0.0 0.0013259559668965621 0.0 3.3111014716217007 3.7884456197044634E-4 80-81 0.0 0.0013259559668965621 0.0 3.630183303941309 3.7884456197044634E-4 82-83 0.0 0.0014206671073891737 0.0 3.9834558579787505 3.7884456197044634E-4 84-85 0.0 0.0015153782478817854 0.0 4.345252414660527 3.7884456197044634E-4 86-87 0.0 0.0015153782478817854 0.0 4.757151164662895 3.7884456197044634E-4 88-89 0.0 0.0015153782478817854 0.0 5.247565450133638 3.7884456197044634E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1330 0.0 67.14286 1 GTATCAA 2105 0.0 42.422802 2 ATCAACG 2740 0.0 32.7646 4 TCAACGC 2730 0.0 32.710625 5 TATCAAC 2750 0.0 32.47273 3 CAACGCA 2820 0.0 31.835104 6 AACGCAG 2840 0.0 31.610914 7 GTATTAT 220 1.8189894E-12 28.068182 1 GTGGTAT 425 0.0 27.941175 1 ACGCAGA 3235 0.0 27.457499 8 CGCAGAG 3245 0.0 26.494608 9 TGGTATC 470 0.0 25.265959 2 GGACCGT 95 0.0012256759 25.000002 6 GTACTAC 175 1.4829675E-7 24.428572 1 GAGTACT 2100 0.0 23.75 12-13 GAGGCGT 120 1.7091092E-4 23.75 6 CAGAGTA 3345 0.0 23.146488 10-11 TAGATAC 125 2.2536593E-4 22.800001 5 GTGTAGC 230 3.616151E-9 22.717392 1 GTACTTT 2140 0.0 22.640186 14-15 >>END_MODULE