FastQCFastQC Report
Fri 10 Feb 2017
SRR2031785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences527921
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27450.5199641613044376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24630.4665470780666046No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT14170.2684113721560612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14130.26765368303212034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15250.062.359371
GTATCAA24650.039.1172182
TCAACGC31250.031.1596585
TATCAAC31500.030.6107753
ATCAACG31750.030.519354
GTGGTAT5800.030.3329931
CAACGCA32150.030.1396396
AACGCAG32200.029.9453227
TGGTATC6400.026.7184582
ACGCAGA36100.026.1839228
CGCAGAG36150.026.0163149
GAGTACT25100.024.22284312-13
CAGAGTA36350.024.04375310-11
TGGACCG1000.001652571423.7497395
GCAGAGT38250.023.346159
GTACTTT26000.023.11032514-15
CCTCGCA1050.002194800422.61889
TACATGG20100.021.977372
GTACATG20250.021.6025161
ATAGTAC1353.792476E-421.1108783
Produced by FastQC (version 0.11.5)