##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031785_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 527921 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.94025242413164 34.0 33.0 34.0 31.0 34.0 2 33.07552455765162 34.0 33.0 34.0 31.0 34.0 3 33.11241265265068 34.0 33.0 34.0 31.0 34.0 4 36.45651906251125 37.0 37.0 37.0 35.0 37.0 5 36.45389366969679 37.0 37.0 37.0 35.0 37.0 6 36.52373745314166 37.0 37.0 37.0 35.0 37.0 7 36.48089960429685 37.0 37.0 37.0 35.0 37.0 8 36.52278465906831 37.0 37.0 37.0 35.0 37.0 9 38.33669999867404 39.0 39.0 39.0 37.0 39.0 10-11 38.3502654753268 39.0 39.0 39.0 37.0 39.0 12-13 38.37006105080116 39.0 39.0 39.0 37.0 39.0 14-15 39.954267778701734 41.0 40.0 41.0 38.0 41.0 16-17 39.927229642313904 41.0 40.0 41.0 38.0 41.0 18-19 39.97761312772177 41.0 40.0 41.0 38.0 41.0 20-21 39.974524597430296 41.0 40.0 41.0 38.0 41.0 22-23 39.94014918898851 41.0 40.0 41.0 38.0 41.0 24-25 39.87446133038845 41.0 40.0 41.0 38.0 41.0 26-27 39.75439791180878 41.0 40.0 41.0 37.0 41.0 28-29 39.707416450567415 41.0 40.0 41.0 37.0 41.0 30-31 39.54737830092002 41.0 40.0 41.0 36.5 41.0 32-33 39.07578406617657 40.5 39.0 41.0 35.0 41.0 34-35 39.10991985543292 40.5 39.0 41.0 35.0 41.0 36-37 39.059775042099105 41.0 39.0 41.0 35.0 41.0 38-39 38.960213744101864 41.0 39.0 41.0 35.0 41.0 40-41 38.84346521543943 41.0 39.0 41.0 35.0 41.0 42-43 38.67174444661228 40.0 38.0 41.0 35.0 41.0 44-45 38.391717700186206 40.0 38.0 41.0 35.0 41.0 46-47 38.11957755042895 40.0 38.0 41.0 34.0 41.0 48-49 37.911071921745865 40.0 38.0 41.0 33.5 41.0 50-51 37.87186435091614 40.0 38.0 41.0 34.0 41.0 52-53 37.89992157917567 40.5 38.0 41.0 34.0 41.0 54-55 37.78732613402384 40.0 37.5 41.0 34.0 41.0 56-57 37.61534585667174 40.0 37.0 41.0 33.5 41.0 58-59 37.40905268023056 40.0 36.5 41.0 33.0 41.0 60-61 37.17936016941929 40.0 36.0 41.0 33.0 41.0 62-63 36.89656406924521 39.0 35.5 41.0 33.0 41.0 64-65 36.58356174503382 39.0 35.0 41.0 32.0 41.0 66-67 36.26890955275505 38.5 35.0 41.0 32.0 41.0 68-69 35.85344587542454 37.0 35.0 40.0 31.0 41.0 70-71 35.46875858319711 37.0 35.0 39.5 31.0 41.0 72-73 35.06390634204739 36.0 35.0 39.0 31.0 41.0 74-75 34.66197783380468 36.0 35.0 39.0 30.0 40.5 76-77 33.27869700201356 35.0 33.0 36.5 28.0 39.0 78-79 33.92703359025309 35.0 34.5 37.0 29.5 39.0 80-81 33.78002864064889 35.0 35.0 36.5 30.0 39.0 82-83 33.525080457113845 35.0 35.0 36.0 29.0 37.0 84-85 33.31091489067493 35.0 35.0 36.0 29.0 37.0 86-87 33.10948323707524 35.0 34.5 35.5 29.0 37.0 88-89 32.964799657524516 35.0 34.0 35.0 29.0 36.0 90-91 32.80888333671136 35.0 34.0 35.0 29.0 36.0 92-93 32.69903640885663 35.0 34.0 35.0 29.0 36.0 94-95 32.634789106703465 35.0 34.0 35.0 28.5 36.0 96-97 32.560640701923205 35.0 34.0 35.0 27.0 35.0 98-99 32.505582274620636 35.0 34.0 35.0 27.0 35.0 100-101 31.390366172211372 34.5 32.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.508039367820345 1101 2 0.4348499090763909 1101 3 0.38664968240205155 1101 4 0.17122030385816345 1101 5 0.23617324392027683 1101 6 0.2163489966447827 1101 7 0.20277146349503283 1101 8 0.21442972856431908 1101 9 0.2800815056063186 1101 10-11 0.33240204524378925 1101 12-13 0.25637767777399034 1101 14-15 0.29963079575781393 1101 16-17 0.2643119674218539 1101 18-19 0.34213123758843267 1101 20-21 0.35539792943814064 1101 22-23 0.3693264645071821 1101 24-25 0.3800202711757237 1101 26-27 0.40447221696894076 1101 28-29 0.27203917044805337 1101 30-31 0.4182742714914198 1101 32-33 0.24826578887847006 1101 34-35 0.23519740749830476 1101 36-37 0.48377860654792215 1101 38-39 0.42800499283336535 1101 40-41 0.3845329079290707 1101 42-43 0.36784141579239105 1101 44-45 0.28952623671869304 1101 46-47 0.3937259433849576 1101 48-49 0.4798303055466775 1101 50-51 0.45162736015838334 1101 52-53 0.43574638836441437 1101 54-55 0.49608457690614927 1101 56-57 0.5689405902119589 1101 58-59 0.4247089170629863 1101 60-61 0.47300327077569193 1101 62-63 0.4225858451237272 1101 64-65 0.4243769875431198 1101 66-67 0.4912241422699495 1101 68-69 0.28868831446919074 1101 70-71 0.1573932606053745 1101 72-73 0.3092457725014768 1101 74-75 0.12346666320727451 1101 76-77 0.3205334739980472 1101 78-79 0.2761226506003496 1101 80-81 0.40030217601492524 1101 82-83 0.4059745801273493 1101 84-85 0.2897224897247668 1101 86-87 0.1660037110465069 1101 88-89 0.22614155580600226 1101 90-91 0.24724460173086982 1101 92-93 0.19079743729726317 1101 94-95 0.17225639361135592 1101 96-97 0.3427735554266249 1101 98-99 0.2452966181455949 1101 100-101 0.1783511951097232 1102 1 0.32427868405966365 1102 2 0.2810037552302376 1102 3 0.2727785997809633 1102 4 0.21253084516870047 1102 5 0.21480572255275376 1102 6 0.15776637556216144 1102 7 0.14917459739816508 1102 8 0.09397032685491524 1102 9 0.24268834321315325 1102 10-11 0.21007298541473318 1102 12-13 0.26821884586516376 1102 14-15 0.29486761911963555 1102 16-17 0.19940812126800722 1102 18-19 0.17769035439754788 1102 20-21 0.2098762057914172 1102 22-23 0.12426770382342056 1102 24-25 0.21206265009310954 1102 26-27 0.19587178961852203 1102 28-29 0.28098682002070774 1102 30-31 0.18701536835751398 1102 32-33 0.2731945638072375 1102 34-35 0.2144531055039991 1102 36-37 0.3659456293399401 1102 38-39 0.3916356908390597 1102 40-41 0.45638119852736025 1102 42-43 0.28450808245906245 1102 44-45 0.4192448976873493 1102 46-47 0.5557629804219957 1102 48-49 0.8125404195067958 1102 50-51 0.6883171749731929 1102 52-53 0.5388891946322261 1102 54-55 0.6922651324617064 1102 56-57 0.6669640944854578 1102 58-59 0.7429069227609943 1102 60-61 0.7257632422856588 1102 62-63 0.6150270844399657 1102 64-65 0.671928625719751 1102 66-67 0.5658324043782201 1102 68-69 0.6538058358367138 1102 70-71 0.7543038731409837 1102 72-73 0.8396944904501993 1102 74-75 0.7334648825804919 1102 76-77 0.893939813029391 1102 78-79 0.823576354304052 1102 80-81 0.9779375036502529 1102 82-83 0.679745947648712 1102 84-85 0.6585348116620935 1102 86-87 0.6963188819866772 1102 88-89 0.5966454731849282 1102 90-91 0.6663151145513879 1102 92-93 0.7110965825964115 1102 94-95 0.9503457810757467 1102 96-97 0.7525402933183543 1102 98-99 0.5219169885159616 1102 100-101 0.4469587449672723 1103 1 0.21844660763935053 1103 2 0.24763271450625268 1103 3 0.2818702706373415 1103 4 0.08864111833779731 1103 5 0.07723659233656122 1103 6 0.07833994687103285 1103 7 0.22285064901539187 1103 8 0.1498086878403342 1103 9 0.21560186759726463 1103 10-11 0.1820909592709441 1103 12-13 0.1299636506246742 1103 14-15 0.07472977765826272 1103 16-17 0.15741151493316607 1103 18-19 0.21656562672870194 1103 20-21 0.17659419640646234 1103 22-23 0.2025419622447373 1103 24-25 0.15511925307617958 1103 26-27 0.04120864230831245 1103 28-29 0.1391913197693171 1103 30-31 0.09283592307512833 1103 32-33 0.13288117349384976 1103 34-35 -0.03375055630259993 1103 36-37 0.07731650247551869 1103 38-39 0.037521395548296255 1103 40-41 0.08108268168472677 1103 42-43 -0.040105416786794024 1103 44-45 0.08406809192230469 1103 46-47 -0.24385889824400664 1103 48-49 -0.09143666277910967 1103 50-51 -0.036706915859717526 1103 52-53 -0.2885890188754061 1103 54-55 -0.08090920137438928 1103 56-57 -0.0576148396522953 1103 58-59 -0.05224244040307724 1103 60-61 -0.3083881319392461 1103 62-63 -0.22680612320206706 1103 64-65 -0.14780909842973244 1103 66-67 -0.10323287130470504 1103 68-69 0.0929869570031272 1103 70-71 -0.03894440907788521 1103 72-73 -0.06476440849399268 1103 74-75 -0.015885712358340243 1103 76-77 -0.14418654862639357 1103 78-79 -0.04217486071186727 1103 80-81 0.04099787737239069 1103 82-83 0.024187249810609046 1103 84-85 -0.1843301120851919 1103 86-87 -0.1529301124829061 1103 88-89 -0.10891217722566893 1103 90-91 -0.207223724060988 1103 92-93 -0.023993286684643067 1103 94-95 -0.14045571046103333 1103 96-97 -0.06411275679057127 1103 98-99 0.18350363172025652 1103 100-101 -0.06570989086312551 1104 1 0.23888991262437997 1104 2 0.20832933662558872 1104 3 0.1875268575524771 1104 4 0.12222859788970908 1104 5 0.07970471245174338 1104 6 0.10250934792141919 1104 7 0.04688229452446535 1104 8 -0.05383035437599659 1104 9 0.11692489477528767 1104 10-11 0.06407081252302049 1104 12-13 0.10522505133067028 1104 14-15 0.19025071363063262 1104 16-17 0.11926805784305827 1104 18-19 0.11331354365445634 1104 20-21 0.10297629583917711 1104 22-23 -0.004065042241883532 1104 24-25 0.07402285289632715 1104 26-27 0.1431644840042381 1104 28-29 0.04058131144659427 1104 30-31 0.03633462683374944 1104 32-33 0.38011649631056343 1104 34-35 0.2765151014322811 1104 36-37 0.26192088578032013 1104 38-39 0.09475212651364018 1104 40-41 0.2897512361125152 1104 42-43 0.234054417906556 1104 44-45 0.6467133809697785 1104 46-47 0.6732581843997565 1104 48-49 0.8516793866980876 1104 50-51 1.0922523601583833 1104 52-53 0.8952189652061833 1104 54-55 0.7070716276461084 1104 56-57 0.6217591379432932 1104 58-59 0.7090386861311302 1104 60-61 0.7430166875914281 1104 62-63 0.6881346132160928 1104 64-65 1.0015719582700626 1104 66-67 0.9722169866348906 1104 68-69 0.9348502920265176 1104 70-71 0.8077403698914338 1104 72-73 0.514091072550265 1104 74-75 0.6610057955865258 1104 76-77 0.5248222811211605 1104 78-79 0.6947933694164163 1104 80-81 0.63592483823642 1104 82-83 0.5840726448533218 1104 84-85 0.8029538078252756 1104 86-87 0.7400630093063896 1104 88-89 0.6156484878274782 1104 90-91 0.7267229721976136 1104 92-93 0.6908431764418381 1104 94-95 0.6794145697375669 1104 96-97 0.7870251613788071 1104 98-99 0.6553785420356562 1104 100-101 0.647258741052152 1105 1 0.4196967825705471 1105 2 0.34105530162199216 1105 3 0.27050674029261046 1105 4 0.11392450687164057 1105 5 0.22290996081162717 1105 6 0.16670585627999657 1105 7 0.15720247063222104 1105 8 0.11769598550325355 1105 9 0.2887650900630945 1105 10-11 0.20677258449835278 1105 12-13 0.15270004097779832 1105 14-15 0.1591049234342563 1105 16-17 0.32357049240202684 1105 18-19 0.27948254607375844 1105 20-21 0.2259247930384518 1105 22-23 0.14945384357141478 1105 24-25 0.16939130606866826 1105 26-27 0.15718335891978086 1105 28-29 0.1378525130333017 1105 30-31 0.049026154917264364 1105 32-33 0.32649735113064793 1105 34-35 0.14436671756967456 1105 36-37 0.17915677863356194 1105 38-39 0.12741270496659496 1105 40-41 0.07746510459838163 1105 42-43 -0.018047759465339652 1105 44-45 -0.14608538104499047 1105 46-47 -0.019998283419162988 1105 48-49 -0.09066929794261824 1105 50-51 -0.10465485633646665 1105 52-53 -0.22327670838419778 1105 54-55 -0.22791671175364314 1105 56-57 -0.1563320108982751 1105 58-59 -0.12382894970941294 1105 60-61 -0.1543460551561111 1105 62-63 -0.21616564179142017 1105 64-65 -0.03625197756394272 1105 66-67 -0.1377806158585173 1105 68-69 -0.0379968560304107 1105 70-71 -0.06809494320113174 1105 72-73 -0.15955379133674086 1105 74-75 -0.1352540941281788 1105 76-77 -0.22114227937229458 1105 78-79 -0.1972665246573868 1105 80-81 -0.32396923556318313 1105 82-83 -0.15110313597098468 1105 84-85 -0.13784739528712464 1105 86-87 -0.18997120965135394 1105 88-89 -0.12302329351992825 1105 90-91 -0.016266500568896447 1105 92-93 0.03507281398242412 1105 94-95 -0.23318323763368198 1105 96-97 -0.036823490568615114 1105 98-99 -0.1225513199987347 1105 100-101 -0.15320114430344134 1106 1 0.1126331708109447 1106 2 0.1504927331861481 1106 3 0.26942003079113164 1106 4 0.18189550040510483 1106 5 0.16150467601523388 1106 6 0.2306853616825535 1106 7 0.2431310287794446 1106 8 0.23557297659884568 1106 9 0.3148047983456408 1106 10-11 0.24450991482911633 1106 12-13 0.12637729238783635 1106 14-15 0.2472399563867569 1106 16-17 0.24055291084717112 1106 18-19 0.23846236137446652 1106 20-21 0.16215808436337653 1106 22-23 0.15022239097550738 1106 24-25 0.2194129989389424 1106 26-27 0.19573262239518385 1106 28-29 0.13336518786750418 1106 30-31 0.23089711332894325 1106 32-33 0.4483968201718227 1106 34-35 0.06819995104694243 1106 36-37 0.22306728004876675 1106 38-39 0.3313670053198763 1106 40-41 0.17419877813101436 1106 42-43 0.17927678653386891 1106 44-45 0.19160443156992812 1106 46-47 0.43061221593004717 1106 48-49 0.4886171099246681 1106 50-51 0.4539758070521742 1106 52-53 0.3659553640080162 1106 54-55 0.4014265107738879 1106 56-57 0.34157720960458704 1106 58-59 0.29580206489711713 1106 60-61 0.18310616887958986 1106 62-63 0.31952636077040353 1106 64-65 0.2793007774305849 1106 66-67 0.2552714676900223 1106 68-69 0.4061925537169202 1106 70-71 0.4636716828344447 1106 72-73 0.5268396140306848 1106 74-75 0.4233910311739706 1106 76-77 0.1606584354594247 1106 78-79 0.25677144819554343 1106 80-81 0.26831223459362263 1106 82-83 0.3578591184347317 1106 84-85 0.18296329826492297 1106 86-87 0.3254451901585753 1106 88-89 0.38173880800270865 1106 90-91 0.27513692483862684 1106 92-93 0.3172185487509367 1106 94-95 0.30585773090132307 1106 96-97 0.1369364003472313 1106 98-99 0.4780746742578188 1106 100-101 0.45073249617339073 1107 1 0.2348504208449924 1107 2 0.1681396775603332 1107 3 0.1658925875551489 1107 4 0.14243016196122227 1107 5 0.18473560389040244 1107 6 0.07818618857906756 1107 7 0.14449103033595634 1107 8 0.05037539697357829 1107 9 0.19498068409772884 1107 10-11 0.11355869199510238 1107 12-13 -0.005282142987766747 1107 14-15 -0.057069633667133246 1107 16-17 0.09073093680198951 1107 18-19 0.051317197259827196 1107 20-21 -0.020897346887473134 1107 22-23 -0.05630133609027865 1107 24-25 0.012640230100295469 1107 26-27 0.041799513458862236 1107 28-29 -0.05773351439137997 1107 30-31 0.031080003380886012 1107 32-33 0.15241067312044265 1107 34-35 0.04433669054535727 1107 36-37 0.10999453261214143 1107 38-39 -0.03788606392540572 1107 40-41 0.042944035636317324 1107 42-43 0.1278535517147148 1107 44-45 0.15976988869777387 1107 46-47 0.11587400163738693 1107 48-49 0.3878213436348048 1107 50-51 0.2262329426826568 1107 52-53 0.2507599245854806 1107 54-55 0.17378481962459347 1107 56-57 0.16678879782958234 1107 58-59 0.12497497382175737 1107 60-61 -0.020135265445375694 1107 62-63 0.10745561734179887 1107 64-65 0.10699694646768876 1107 66-67 0.14654527744546186 1107 68-69 0.1350288671204254 1107 70-71 0.17428319712516327 1107 72-73 0.3388247493498753 1107 74-75 0.33449868337157085 1107 76-77 0.19613326862089764 1107 78-79 0.2457221651634569 1107 80-81 0.23462349789692638 1107 82-83 0.24240101776737788 1107 84-85 0.3335168325178941 1107 86-87 0.5041735206058817 1107 88-89 0.5022594609591025 1107 90-91 0.5092965061371402 1107 92-93 0.4293476613450622 1107 94-95 0.4641043211692377 1107 96-97 0.3123007212219946 1107 98-99 0.44462914241744045 1107 100-101 0.3559370800985242 1108 1 0.11005230815104738 1108 2 0.03310655896855508 1108 3 0.00920989088515256 1108 4 0.022586227653988544 1108 5 -0.11808628160093093 1108 6 -0.11736775389439202 1108 7 -0.07939065009231427 1108 8 -0.12489000543999396 1108 9 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sequence quality scores pass #Quality Count 9 2.0 10 0.0 11 1.0 12 3.0 13 2.0 14 33.0 15 79.0 16 205.0 17 526.0 18 949.0 19 1407.0 20 2237.0 21 3408.0 22 4700.0 23 4758.0 24 4166.0 25 3429.0 26 2948.0 27 3003.0 28 3618.0 29 4125.0 30 4453.0 31 5324.0 32 6643.0 33 9035.0 34 13332.0 35 23145.0 36 49267.0 37 122780.0 38 196345.0 39 57933.0 40 65.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.08099156622392 18.352094027308286 13.914592367489556 23.652322038978234 2 16.828654287289197 20.67260063532233 38.23109897124759 24.26764610614088 3 18.149874697161128 25.26324961499921 31.402615164011284 25.184260523828378 4 13.21921272311577 18.268074200495906 36.52345710816581 31.98925596822252 5 13.76247582498139 36.7363677519932 34.716747392128745 14.784409030896667 6 30.76994474552064 36.45261317507733 17.54239744204152 15.235044637360515 7 26.186493812521196 33.25705929485662 21.92809151369239 18.6283553789298 8 25.843828906218924 33.287177437533266 20.815803879747158 20.053189776500652 9 26.575377755383855 14.690834424089969 19.982345843412176 38.751441977114 10-11 24.546949259453594 25.458070430992514 28.524722449002788 21.470257860551108 12-13 25.806986272567293 24.082959382180288 27.92766341933736 22.18239092591505 14-15 23.308979942074668 25.143250599995078 26.19511252630602 25.35265693162424 16-17 22.072241869522145 27.811642272233918 27.102255829944255 23.01386002829969 18-19 21.99012731071505 26.131750773316465 29.670537826682402 22.207584089286087 20-21 22.95324489838442 25.368284269805518 29.6577518227159 22.020719009094165 22-23 22.70898486705397 25.55799068421222 29.418606192972053 22.314418255761755 24-25 22.415380331526876 25.92139733028237 29.524587959183286 22.138634379007467 26-27 22.209004756393476 25.90728537034897 29.69592041233442 22.18778946092313 28-29 21.968154326120764 25.91012670456375 29.879375891468612 22.242343077846876 30-31 22.646570225469343 25.677705565794884 29.632274525923386 22.04344968281239 32-33 22.15767131824648 25.92802711011685 29.53604800718289 22.37825356445377 34-35 22.50980733859801 26.123510904093607 29.444083489764566 21.92259826754382 36-37 22.298696206439978 26.156943936687497 29.399379831452055 22.14498002542047 38-39 22.0830389395383 26.044711235203756 29.60083042727984 22.271419397978107 40-41 22.28126935658934 25.896393589192325 29.594200647445355 22.228136406772983 42-43 22.274334791264536 25.97557949450387 29.287084901603848 22.463000812627744 44-45 22.417937532320174 26.358773377077256 28.955847560525154 22.267441530077416 46-47 22.478974086982877 26.18427034399151 28.679818911956357 22.656936657069256 48-49 22.514169028171363 26.40894694570307 28.38238191213687 22.694502113988698 50-51 22.51619814155864 26.69129813388696 28.19646325974517 22.596040464809235 52-53 22.537664431157463 26.79267727878451 28.116745293070807 22.552912996987224 54-55 22.516247696151506 26.759496212501492 27.80387595871352 22.92038013263348 56-57 22.5471235279521 26.947592537519817 27.583483134787212 22.921800799740872 58-59 22.35135560055387 26.95261222796593 28.0797695109685 22.616262660511705 60-61 22.524961121076828 27.11040098802662 27.82376529821697 22.540872592679587 62-63 22.247078634871507 27.2427124512948 27.892336163933617 22.61787274990008 64-65 22.2932976714319 27.156904158008487 27.70016725987411 22.8496309106855 66-67 22.239880588194065 27.214014975725537 27.68889663415549 22.857207801924908 68-69 22.288541569981646 27.18088260086339 27.56569688038082 22.964878948774146 70-71 22.176329412923522 26.996179352592524 27.650538622255983 23.176952612227968 72-73 22.22472680571525 27.0952472055478 27.43649144474268 23.243534543994272 74-75 22.283353001680176 27.22291782293184 27.237124494005734 23.256604681382253 76-77 22.19896537550126 27.15141091185992 27.427683308676865 23.22194040396196 78-79 22.230030629582835 27.102445252225237 27.33060438967194 23.336919728519987 80-81 22.145548292263424 27.15538877976061 27.236177382600808 23.46288554537516 82-83 22.153125183502834 27.100929873977357 27.25332009902997 23.49262484348984 84-85 22.114388327041357 27.09818325090307 27.212689019758642 23.574739402296935 86-87 22.03255790165574 27.103771208192136 27.23077884759273 23.632892042559398 88-89 22.092320631306578 27.008681223137554 27.18389683304888 23.715101312506984 90-91 22.083228361819287 27.00792353401361 27.10538129758051 23.80346680658659 92-93 21.98936962159111 26.897300921918244 27.18399154418938 23.92933791230127 94-95 21.943529429592683 26.808746005557648 27.140045575000805 24.10767898984886 96-97 21.933679470981453 26.86642509011765 27.227179824254005 23.972715614646887 98-99 21.88348256652037 26.95062329401558 26.985950549419325 24.179943590044722 100-101 21.77647960251591 27.03626777137846 27.050569167137855 24.13668345896778 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 47.0 1 39.5 2 54.0 3 78.5 4 66.5 5 64.0 6 109.0 7 244.5 8 456.0 9 636.5 10 629.5 11 545.5 12 575.5 13 616.0 14 651.0 15 732.0 16 790.0 17 825.5 18 889.5 19 952.5 20 1029.0 21 1118.5 22 1340.0 23 1603.5 24 1841.0 25 2197.0 26 2692.5 27 3220.5 28 3819.5 29 4501.5 30 5243.0 31 6205.5 32 7182.5 33 8038.0 34 8935.0 35 9844.0 36 10988.5 37 12192.5 38 13221.0 39 14166.5 40 15073.5 41 16090.0 42 17008.0 43 18000.5 44 18872.5 45 19699.0 46 20496.0 47 21148.0 48 21866.0 49 22069.5 50 21739.5 51 21179.5 52 20342.5 53 19521.5 54 18503.5 55 17161.0 56 15448.5 57 13529.0 58 12157.0 59 10652.5 60 8735.5 61 7047.0 62 5604.5 63 4247.0 64 3175.0 65 2403.0 66 1822.0 67 1453.0 68 1158.0 69 858.5 70 613.5 71 488.0 72 396.0 73 300.0 74 207.5 75 166.0 76 124.5 77 71.5 78 46.5 79 29.0 80 13.0 81 11.5 82 13.0 83 9.5 84 4.5 85 1.5 86 3.0 87 2.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1430138221438435 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 7.576891239408927E-4 44-45 0.0 46-47 1.8942228098522317E-4 48-49 0.0017048005288670086 50-51 0.0010418225454187273 52-53 4.7355570246305795E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 7.576891239408927E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 9.471114049261159E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 527921.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.0962752567257 #Duplication Level Percentage of deduplicated Percentage of total 1 81.61099713045627 61.286819044845956 2 13.20597626311656 19.834392569775744 3 3.020879500851431 6.805703955400176 4 1.0030734086171922 3.0130830718487505 5 0.44690624733743894 1.6780497282001328 6 0.22798066756315252 1.027229937872075 7 0.12870481770095163 0.6765676691856156 8 0.08274964726931355 0.4971352230986665 9 0.05797179834405827 0.39181195130155155 >10 0.20261009786576192 2.271222786306209 >50 0.006328348433009119 0.3262014709374262 >100 0.00455640452207891 0.565719426842643 >500 2.531335845597786E-4 0.0980884490089656 >1k 0.0010125343382391145 1.5279747153760956 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2745 0.5199641613044376 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2463 0.4665470780666046 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 1417 0.2684113721560612 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1413 0.26765368303212034 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.8942228098522317E-4 0.0 0.0 0.0 3 0.0 1.8942228098522317E-4 0.0 0.0 0.0 4 0.0 1.8942228098522317E-4 0.0 0.0 0.0 5 0.0 1.8942228098522317E-4 0.0 0.0 0.0 6 0.0 1.8942228098522317E-4 0.0 0.0 0.0 7 0.0 1.8942228098522317E-4 0.0 0.0 0.0 8 0.0 1.8942228098522317E-4 0.0 0.0 0.0 9 0.0 1.8942228098522317E-4 0.0 0.0 0.0 10-11 0.0 1.8942228098522317E-4 0.0 0.0 0.0 12-13 0.0 1.8942228098522317E-4 0.0 0.0 0.0 14-15 0.0 1.8942228098522317E-4 0.0 0.0 0.0 16-17 0.0 1.8942228098522317E-4 0.0 0.0 0.0 18-19 0.0 2.8413342147783473E-4 0.0 9.471114049261159E-5 0.0 20-21 0.0 3.7884456197044634E-4 0.0 3.7884456197044634E-4 3.7884456197044634E-4 22-23 0.0 3.7884456197044634E-4 0.0 5.682668429556695E-4 3.7884456197044634E-4 24-25 0.0 3.7884456197044634E-4 0.0 0.0018942228098522316 3.7884456197044634E-4 26-27 0.0 3.7884456197044634E-4 0.0 0.004356712462660133 3.7884456197044634E-4 28-29 0.0 3.7884456197044634E-4 0.0 0.013164848528473012 3.7884456197044634E-4 30-31 0.0 3.7884456197044634E-4 0.0 0.026329697056946023 3.7884456197044634E-4 32-33 0.0 3.7884456197044634E-4 0.0 0.04877623735369496 3.7884456197044634E-4 34-35 0.0 3.7884456197044634E-4 0.0 0.0874183826746805 3.7884456197044634E-4 36-37 0.0 3.7884456197044634E-4 0.0 0.140456621350543 3.7884456197044634E-4 38-39 0.0 4.735557024630579E-4 0.0 0.20561788600945974 3.7884456197044634E-4 40-41 0.0 5.682668429556695E-4 0.0 0.27011617268492827 3.7884456197044634E-4 42-43 0.0 7.576891239408927E-4 0.0 0.33082601374069226 3.7884456197044634E-4 44-45 0.0 7.576891239408927E-4 0.0 0.4011963911267027 3.7884456197044634E-4 46-47 0.0 7.576891239408927E-4 0.0 0.49647579846226997 3.7884456197044634E-4 48-49 0.0 7.576891239408927E-4 0.0 0.589860982987985 3.7884456197044634E-4 50-51 0.0 7.576891239408927E-4 0.0 0.6815413669848329 3.7884456197044634E-4 52-53 0.0 7.576891239408927E-4 0.0 0.7697174387834543 3.7884456197044634E-4 54-55 0.0 7.576891239408927E-4 0.0 0.8774987166640463 3.7884456197044634E-4 56-57 0.0 7.576891239408927E-4 0.0 1.0128409364279882 3.7884456197044634E-4 58-59 0.0 7.576891239408927E-4 0.0 1.1561388919933095 3.7884456197044634E-4 60-61 0.0 7.576891239408927E-4 0.0 1.2787898189312417 5.682668429556695E-4 62-63 0.0 7.576891239408927E-4 0.0 1.3935797212082868 7.576891239408927E-4 64-65 0.0 7.576891239408927E-4 0.0 1.5515579035499631 7.576891239408927E-4 66-67 0.0 9.471114049261158E-4 0.0 1.7574599229809005 7.576891239408927E-4 68-69 0.0 9.471114049261158E-4 0.0 1.9959425747412967 7.576891239408927E-4 70-71 0.0 9.471114049261158E-4 0.0 2.2393502058073085 7.576891239408927E-4 72-73 0.0 9.471114049261158E-4 0.0 2.490050594691251 8.524002644335043E-4 74-75 0.0 9.471114049261158E-4 0.0 2.7513586313103664 9.471114049261158E-4 76-77 0.0 0.001136533685911339 0.0 3.0327454297139154 9.471114049261158E-4 78-79 0.0 0.001136533685911339 0.0 3.338378280083573 9.471114049261158E-4 80-81 0.0 0.0012312448264039505 0.0 3.6621009582873194 9.471114049261158E-4 82-83 0.0 0.0013259559668965621 0.0 4.014047556357864 9.471114049261158E-4 84-85 0.0 0.0013259559668965621 0.0 4.377833046989985 9.471114049261158E-4 86-87 0.0 0.0013259559668965621 0.0 4.793141398050087 0.0010418225454187273 88-89 0.0 0.0013259559668965621 0.0 5.284786928347234 0.001136533685911339 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1525 0.0 62.35937 1 GTATCAA 2465 0.0 39.117218 2 TCAACGC 3125 0.0 31.159658 5 TATCAAC 3150 0.0 30.610775 3 ATCAACG 3175 0.0 30.51935 4 GTGGTAT 580 0.0 30.332993 1 CAACGCA 3215 0.0 30.139639 6 AACGCAG 3220 0.0 29.945322 7 TGGTATC 640 0.0 26.718458 2 ACGCAGA 3610 0.0 26.183922 8 CGCAGAG 3615 0.0 26.016314 9 GAGTACT 2510 0.0 24.222843 12-13 CAGAGTA 3635 0.0 24.043753 10-11 TGGACCG 100 0.0016525714 23.749739 5 GCAGAGT 3825 0.0 23.34615 9 GTACTTT 2600 0.0 23.110325 14-15 CCTCGCA 105 0.0021948004 22.6188 9 TACATGG 2010 0.0 21.97737 2 GTACATG 2025 0.0 21.602516 1 ATAGTAC 135 3.792476E-4 21.110878 3 >>END_MODULE