FastQCFastQC Report
Fri 10 Feb 2017
SRR2031783_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031783_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574799
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46780.2970537827367175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40120.25476267129963887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24150.15335290408490226No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21200.055.566041
GTATCAA36200.041.0704421
TCAACGC43000.034.133724
ATCAACG43600.033.663993
CAACGCA44100.033.605445
AACGCAG45750.032.3934446
TATCAAC46300.031.8034552
ACGCAGA50700.028.7623277
CGCAGAG51450.028.6200228
GTACATG31950.026.1658841
TACATGG32450.024.738062
GCAGAGT60400.024.064579
ACATGGG34050.022.3201163
GAGTACT43250.022.07514612-13
AGAGTAC52900.021.95415710-11
CAGAGTA58450.021.81993110-11
CATGGGG21950.020.3416884
AGTACTT46000.019.92934812-13
GTACTTT49500.018.8080814-15
CGGTTCG901.2341855E-418.47222330-31
Produced by FastQC (version 0.11.5)