##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031783_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1574799 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.442712371547096 34.0 31.0 34.0 31.0 34.0 2 32.58166470768651 34.0 31.0 34.0 31.0 34.0 3 32.65648568483978 34.0 31.0 34.0 31.0 34.0 4 35.8671792400173 37.0 37.0 37.0 35.0 37.0 5 35.8906171517762 37.0 37.0 37.0 35.0 37.0 6 35.963265788205355 37.0 37.0 37.0 35.0 37.0 7 35.983530596603124 37.0 37.0 37.0 35.0 37.0 8 35.97914273504111 37.0 37.0 37.0 35.0 37.0 9 37.79787388739769 39.0 39.0 39.0 35.0 39.0 10-11 37.82338984213224 39.0 39.0 39.0 35.0 39.0 12-13 37.793532063456986 39.0 39.0 39.0 35.0 39.0 14-15 39.347013491880546 41.0 40.0 41.0 37.0 41.0 16-17 39.28150449676435 41.0 40.0 41.0 36.0 41.0 18-19 39.28451630970048 41.0 40.0 41.0 36.5 41.0 20-21 39.2282729414992 41.0 40.0 41.0 36.0 41.0 22-23 39.137417219594376 41.0 39.5 41.0 36.0 41.0 24-25 39.098701485078415 41.0 39.0 41.0 36.0 41.0 26-27 38.98279748717138 41.0 39.0 41.0 35.5 41.0 28-29 38.86141374232521 41.0 39.0 41.0 35.0 41.0 30-31 38.70979375780655 40.5 39.0 41.0 35.0 41.0 32-33 38.58637515009852 40.0 38.0 41.0 35.0 41.0 34-35 38.3990293364423 40.0 38.0 41.0 34.0 41.0 36-37 38.27766305414215 40.0 38.0 41.0 34.0 41.0 38-39 38.100355664437174 40.0 38.0 41.0 33.5 41.0 40-41 38.01163926316946 40.0 38.0 41.0 33.0 41.0 42-43 37.849000412116084 40.0 38.0 41.0 33.0 41.0 44-45 38.015107007306966 40.0 38.0 41.0 33.0 41.0 46-47 37.898626110379794 40.0 38.0 41.0 33.0 41.0 48-49 37.735698651065945 40.0 38.0 41.0 33.0 41.0 50-51 36.68528205821822 39.0 36.5 40.0 31.0 40.5 52-53 36.72021667527094 39.5 37.0 40.0 31.0 40.5 54-55 37.51716790523743 40.0 38.0 41.0 32.0 41.0 56-57 37.52281211760993 40.0 37.0 41.0 32.0 41.0 58-59 37.40888202240413 40.0 37.0 41.0 32.0 41.0 60-61 37.21725661497118 40.0 36.5 41.0 31.5 41.0 62-63 36.95962119610185 39.5 36.0 41.0 31.0 41.0 64-65 36.64781314948765 39.0 35.5 41.0 31.0 41.0 66-67 36.383306377512305 39.0 35.0 41.0 30.5 41.0 68-69 36.059844780191 38.5 35.0 41.0 30.0 41.0 70-71 35.70609646056417 37.0 35.0 40.0 30.0 41.0 72-73 35.304785245609125 37.0 35.0 39.5 29.0 41.0 74-75 34.95036445921035 36.0 35.0 39.0 29.0 41.0 76-77 34.56410849892589 36.0 35.0 39.0 29.0 40.5 78-79 34.190652267368726 35.0 34.5 37.0 29.0 39.0 80-81 33.83651723172291 35.0 34.0 37.0 28.0 39.0 82-83 33.54253685708462 35.0 34.0 36.5 28.0 39.0 84-85 33.24034019579642 35.0 34.0 36.0 27.0 37.0 86-87 32.98005554994637 35.0 34.0 36.0 26.5 37.0 88-89 32.776613078875464 35.0 34.0 35.5 26.0 37.0 90-91 32.6029867938702 35.0 34.0 35.0 26.0 36.0 92-93 32.47602805183392 35.0 34.0 35.0 26.0 36.0 94-95 32.32006021085866 35.0 34.0 35.0 25.0 36.0 96-97 32.22078912927935 35.0 34.0 35.0 25.0 36.0 98-99 32.11215685303331 35.0 33.5 35.0 25.0 35.0 100-101 30.60666821607075 34.0 30.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2871174373100729 1101 2 0.18796424289674718 1101 3 0.21466104934676622 1101 4 0.2624015045573955 1101 5 0.1800764941136137 1101 6 0.21045524254627423 1101 7 0.25849581101215335 1101 8 0.2103599959406921 1101 9 0.23849910387426831 1101 10-11 0.3173088680369247 1101 12-13 0.31966992123080473 1101 14-15 0.38103539367446615 1101 16-17 0.4446991914914946 1101 18-19 0.5252591240276274 1101 20-21 0.42789417165926835 1101 22-23 0.4560479804085631 1101 24-25 0.3745201292430238 1101 26-27 0.2748252466404608 1101 28-29 0.43272408945745156 1101 30-31 0.46900031952147714 1101 32-33 0.3562610953691703 1101 34-35 0.4692648094643772 1101 36-37 0.44811360300777636 1101 38-39 0.45547305688554474 1101 40-41 0.3762678717550543 1101 42-43 0.3427494295757114 1101 44-45 0.5640246835571006 1101 46-47 0.5457037478479521 1101 48-49 0.5251224299300006 1101 50-51 0.5145251836647162 1101 52-53 0.5770478864005 1101 54-55 0.6235738787756588 1101 56-57 0.6004978230586033 1101 58-59 0.5495114670443542 1101 60-61 0.4863677138962075 1101 62-63 0.5078938625380047 1101 64-65 0.5816777355091247 1101 66-67 0.5113247765552913 1101 68-69 0.512434676204343 1101 70-71 0.4892277062431205 1101 72-73 0.5605901783231033 1101 74-75 0.6056856710085157 1101 76-77 0.5304105339766991 1101 78-79 0.6675566164193611 1101 80-81 0.7593143717090385 1101 82-83 0.5580291376167992 1101 84-85 0.5498984881912818 1101 86-87 0.49768000863957695 1101 88-89 0.4417253412538784 1101 90-91 0.4824283074526363 1101 92-93 0.49768419339351766 1101 94-95 0.4975230609948085 1101 96-97 0.2468756523392699 1101 98-99 0.2968643265562889 1101 100-101 0.36159160216994124 1102 1 0.0951294253220567 1102 2 0.21148294641545107 1102 3 0.22389514858086557 1102 4 0.24698802914392104 1102 5 0.1400565140936365 1102 6 0.21300047509150488 1102 7 0.18887654139287946 1102 8 0.2891090246897221 1102 9 0.36109206646722924 1102 10-11 0.38016268089074146 1102 12-13 0.4284022999631887 1102 14-15 0.45278808542715865 1102 16-17 0.4838861156784233 1102 18-19 0.4605649293334295 1102 20-21 0.40353186729696944 1102 22-23 0.42537920473979085 1102 24-25 0.43850336822625735 1102 26-27 0.5240277108429225 1102 28-29 0.42170455343791247 1102 30-31 0.41622143174258497 1102 32-33 0.47854991765799326 1102 34-35 0.46896344416300906 1102 36-37 0.40911593401011004 1102 38-39 0.5360924375049265 1102 40-41 0.4227558282430053 1102 42-43 0.37708953391581446 1102 44-45 0.5012013957338084 1102 46-47 0.6576345947787985 1102 48-49 0.43479275960032737 1102 50-51 0.6063972005367333 1102 52-53 0.6409295602821743 1102 54-55 0.6950252052269903 1102 56-57 0.6096181471789279 1102 58-59 0.6285396610725513 1102 60-61 0.6304686129970989 1102 62-63 0.6038323684765174 1102 64-65 0.7298884137197987 1102 66-67 0.714733589964105 1102 68-69 0.6607108444805121 1102 70-71 0.6308538578692762 1102 72-73 0.6049952177281384 1102 74-75 0.5884911988140402 1102 76-77 0.31268492625110156 1102 78-79 0.27751721137995844 1102 80-81 0.5220577394524071 1102 82-83 0.5048772894649574 1102 84-85 0.6111749894677772 1102 86-87 0.6855970915566587 1102 88-89 0.5867819513104848 1102 90-91 0.5434589434832731 1102 92-93 0.4479833386926657 1102 94-95 0.5651160235877697 1102 96-97 0.4427714232350439 1102 98-99 0.5071623618543271 1102 100-101 0.4798810904594326 1103 1 0.351571691807365 1103 2 0.3337560981003662 1103 3 0.35427146762289397 1103 4 0.3250943640651798 1103 5 0.4245130905157808 1103 6 0.35033715403506704 1103 7 0.3936574929457137 1103 8 0.3856772898376164 1103 9 0.43817463637620335 1103 10-11 0.3881343839844007 1103 12-13 0.3813386112208761 1103 14-15 0.4066870877479687 1103 16-17 0.47960317559920895 1103 18-19 0.526355029556818 1103 20-21 0.4642124588240435 1103 22-23 0.6094742026798414 1103 24-25 0.5158483490863048 1103 26-27 0.4893584107503841 1103 28-29 0.5238315831115798 1103 30-31 0.4486035641964534 1103 32-33 0.4391768883280065 1103 34-35 0.4514704159095828 1103 36-37 0.4572660144413945 1103 38-39 0.4607649672707126 1103 40-41 0.5192697479276589 1103 42-43 0.5989551463840073 1103 44-45 0.6234248601380514 1103 46-47 0.45790052524558433 1103 48-49 0.5214396134638193 1103 50-51 0.39316164763116035 1103 52-53 0.5692717027563106 1103 54-55 0.5984326140145981 1103 56-57 0.5354702322849576 1103 58-59 0.5631672445523606 1103 60-61 0.6483746667711614 1103 62-63 0.6498748800857328 1103 64-65 0.713083761334083 1103 66-67 0.6712453151271873 1103 68-69 0.6279881471236664 1103 70-71 0.5762920479272609 1103 72-73 0.5808202853753031 1103 74-75 0.5707838941626875 1103 76-77 0.47664053066581147 1103 78-79 0.5069689329407225 1103 80-81 0.5878757887711714 1103 82-83 0.6004631956677855 1103 84-85 0.4775731377884469 1103 86-87 0.6556542940385413 1103 88-89 0.6005251426003113 1103 90-91 0.5835953971778309 1103 92-93 0.632145621491965 1103 94-95 0.5812229837406377 1103 96-97 0.4526669557274232 1103 98-99 0.5786406205635757 1103 100-101 0.630428375153059 1104 1 0.333753566410266 1104 2 0.25500262545874364 1104 3 0.25742048703433085 1104 4 0.2890839665170688 1104 5 0.29174500356446487 1104 6 0.17908105205503944 1104 7 0.1879224418761538 1104 8 0.2546473649713903 1104 9 0.30667580055199295 1104 10-11 0.3523086047820456 1104 12-13 0.27285741135874986 1104 14-15 0.36249382572837874 1104 16-17 0.4218326090663922 1104 18-19 0.4356124120811202 1104 20-21 0.41569441385376393 1104 22-23 0.3492868052387692 1104 24-25 0.33249017083728916 1104 26-27 0.4911157220787814 1104 28-29 0.5026748607942579 1104 30-31 0.4201615368120528 1104 32-33 0.35068798948805835 1104 34-35 0.4102796570397942 1104 36-37 0.4826178940623791 1104 38-39 0.5065760948468991 1104 40-41 0.42814856464190143 1104 42-43 0.521301527717128 1104 44-45 0.5054333604380545 1104 46-47 0.6017056959340863 1104 48-49 0.5918280583194004 1104 50-51 0.538440573545202 1104 52-53 0.5859732799665451 1104 54-55 0.6600101974193677 1104 56-57 0.6700079057206381 1104 58-59 0.563243105016241 1104 60-61 0.6376621381577721 1104 62-63 0.6944162655285879 1104 64-65 0.6630740394105601 1104 66-67 0.6472969768396055 1104 68-69 0.6132555049307129 1104 70-71 0.5917165179105766 1104 72-73 0.5316302983673253 1104 74-75 0.5515902147878009 1104 76-77 0.5698340057821056 1104 78-79 0.5681723695915863 1104 80-81 0.5207125183946602 1104 82-83 0.6222204186150648 1104 84-85 0.587298056453271 1104 86-87 0.7254225980146174 1104 88-89 0.6580816304130437 1104 90-91 0.6146230634153511 1104 92-93 0.5110109453547693 1104 94-95 0.603273337412439 1104 96-97 0.6388766527078289 1104 98-99 0.492621974110655 1104 100-101 0.45581526351886126 1105 1 0.04659855986206196 1105 2 -0.05230226225164358 1105 3 0.0538453820744067 1105 4 0.08995652171871171 1105 5 0.03830786903790795 1105 6 0.1606490644723806 1105 7 0.08750853057605212 1105 8 0.17091925295664367 1105 9 0.23334667100530027 1105 10-11 0.10453378223034093 1105 12-13 0.1450414132558464 1105 14-15 0.1288427831353971 1105 16-17 0.1793488987396259 1105 18-19 0.24994921084369537 1105 20-21 0.2202351771105171 1105 22-23 0.29379480051759543 1105 24-25 0.1916208762650271 1105 26-27 0.18565807522919187 1105 28-29 0.22357306987336045 1105 30-31 0.2853500065798258 1105 32-33 0.22541110546406884 1105 34-35 0.31548831037291336 1105 36-37 0.3130469767206776 1105 38-39 0.38222956809087805 1105 40-41 0.2024126325061033 1105 42-43 0.28208720466151505 1105 44-45 0.4003461049178867 1105 46-47 0.49061996024447296 1105 48-49 0.5722130820600171 1105 50-51 0.59396693613796 1105 52-53 0.507402217109167 1105 54-55 0.5929328531346414 1105 56-57 0.6473492262485934 1105 58-59 0.4675470010641476 1105 60-61 0.4717270920508625 1105 62-63 0.5329079953867861 1105 64-65 0.4788430898398346 1105 66-67 0.41528096472407583 1105 68-69 0.41334119750455756 1105 70-71 0.4784453521143135 1105 72-73 0.5578675859548383 1105 74-75 0.4449224971744741 1105 76-77 0.51843193514771 1105 78-79 0.47667129439231104 1105 80-81 0.4934775051635114 1105 82-83 0.4779786631568754 1105 84-85 0.4983751689907976 1105 86-87 0.517277222449394 1105 88-89 0.4564346083647166 1105 90-91 0.5164026664269983 1105 92-93 0.42589235006624193 1105 94-95 0.5246409693331913 1105 96-97 0.47070969231504733 1105 98-99 0.2621177083451087 1105 100-101 0.24096067142090405 1106 1 0.22255187334035753 1106 2 0.18208739953286113 1106 3 0.1919687416544562 1106 4 0.18473709642666591 1106 5 0.18977557741373374 1106 6 0.1724141903342371 1106 7 0.1420350696809436 1106 8 0.16340901945604003 1106 9 0.2758904948666938 1106 10-11 0.21871381024498504 1106 12-13 0.22043018428382055 1106 14-15 0.28303120874023335 1106 16-17 0.3506019415911368 1106 18-19 0.32312978963145156 1106 20-21 0.3835368579884886 1106 22-23 0.3727797460522524 1106 24-25 0.2957237960425374 1106 26-27 0.36761719562412054 1106 28-29 0.39447677977231876 1106 30-31 0.4023960947307188 1106 32-33 0.4095741683065981 1106 34-35 0.5879017165986937 1106 36-37 0.47996693102691523 1106 38-39 0.5958377440118952 1106 40-41 0.6324695457287461 1106 42-43 0.40537198836406674 1106 44-45 0.4612048350118627 1106 46-47 0.4190636521923068 1106 48-49 0.3616987551591748 1106 50-51 0.3727057336846471 1106 52-53 0.4071385599756283 1106 54-55 0.4515252538254799 1106 56-57 0.387854339599059 1106 58-59 0.3575385694756079 1106 60-61 0.4983316437487346 1106 62-63 0.47615711482976053 1106 64-65 0.5108746267008328 1106 66-67 0.48988097424361143 1106 68-69 0.6543766849727746 1106 70-71 0.5188600298780344 1106 72-73 0.4734718044689785 1106 74-75 0.3741073529535157 1106 76-77 0.514397182043659 1106 78-79 0.49555482287612307 1106 80-81 0.5251792581179728 1106 82-83 0.5590375074852716 1106 84-85 0.4191352358995246 1106 86-87 0.49785737013817766 1106 88-89 0.4222713755304781 1106 90-91 0.44818159561525306 1106 92-93 0.4041937589574971 1106 94-95 0.4245388043188427 1106 96-97 0.44945137972069915 1106 98-99 0.417235988573033 1106 100-101 0.3578231685310449 1107 1 0.12806136164169857 1107 2 0.16045917581828206 1107 3 0.16452206662308555 1107 4 0.09384248515772953 1107 5 0.16944431575245744 1107 6 0.21527813608936697 1107 7 0.16508783880869515 1107 8 0.17117941491569155 1107 9 0.22042748916526733 1107 10-11 0.20163470531132788 1107 12-13 0.1672502706179344 1107 14-15 0.16208258989104962 1107 16-17 0.22276260235917533 1107 18-19 0.23910720818938813 1107 20-21 0.3249919421447487 1107 22-23 0.2775367363201582 1107 24-25 0.24138384896119192 1107 26-27 0.13283943446017332 1107 28-29 0.29591538373406223 1107 30-31 0.2641875601353121 1107 32-33 0.232291401324197 1107 34-35 0.38739674653608347 1107 36-37 0.3267141241929963 1107 38-39 0.4014193941116915 1107 40-41 0.46356542990580607 1107 42-43 0.41742658429050294 1107 44-45 0.41736654245102756 1107 46-47 0.38400748300864507 1107 48-49 0.4444719135705739 1107 50-51 0.3482357328395054 1107 52-53 0.2901344610554517 1107 54-55 0.3506702015244372 1107 56-57 0.28031009986586497 1107 58-59 0.1483904362558235 1107 60-61 0.34376034009059 1107 62-63 0.2925179335736203 1107 64-65 0.238628802008499 1107 66-67 0.41097635458829984 1107 68-69 0.39094833318725364 1107 70-71 0.3736153136169307 1107 72-73 0.4721870319086605 1107 74-75 0.35399712743164 1107 76-77 0.23295275963059936 1107 78-79 0.19141193926465405 1107 80-81 0.19772234391249555 1107 82-83 0.18111040390383693 1107 84-85 0.2182857095019628 1107 86-87 0.1983030180016101 1107 88-89 0.2803881151174039 1107 90-91 0.25500576136634834 1107 92-93 0.2539919822169949 1107 94-95 0.2647902166747613 1107 96-97 0.25089794748452476 1107 98-99 0.35889260567992665 1107 100-101 0.38322850980434353 1108 1 0.10904521613271356 1108 2 -0.012065849260480377 1108 3 0.052910061947855525 1108 4 0.1559166446740079 1108 5 0.24115040797481413 1108 6 0.207180839687517 1108 7 0.19255637147858096 1108 8 0.1891732364898715 1108 9 0.2502482105133481 1108 10-11 0.20596788628029827 1108 12-13 0.1950056368599249 1108 14-15 0.30064889753002433 1108 16-17 0.27043833010349516 1108 18-19 0.29032118910191684 1108 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-0.7186628117411331 2316 54-55 -0.7879477763647742 2316 56-57 -0.660605797078162 2316 58-59 -0.7223289301275315 2316 60-61 -0.8064080462552496 2316 62-63 -1.015336829421969 2316 64-65 -0.8073127215476319 2316 66-67 -0.6571402390920511 2316 68-69 -0.5447035998842082 2316 70-71 -0.6101552864547912 2316 72-73 -0.4785314459090202 2316 74-75 -0.8988147732985539 2316 76-77 -1.0276441737465163 2316 78-79 -0.69732593197147 2316 80-81 -0.6184515364159893 2316 82-83 -0.6239345064739155 2316 84-85 -0.6156453050320678 2316 86-87 -0.5276661645242768 2316 88-89 -0.5602487343942286 2316 90-91 -0.4189731916007986 2316 92-93 -0.7894322536953027 2316 94-95 -0.8538865514921525 2316 96-97 -0.8615922609628939 2316 98-99 -0.9412543227502042 2316 100-101 -0.9003258726204599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 11.0 7 344.0 8 924.0 9 866.0 10 876.0 11 1297.0 12 1801.0 13 2103.0 14 2252.0 15 2567.0 16 2860.0 17 3691.0 18 4639.0 19 5902.0 20 7495.0 21 9701.0 22 11077.0 23 11653.0 24 11084.0 25 10153.0 26 9902.0 27 10404.0 28 11904.0 29 13344.0 30 16093.0 31 19861.0 32 25029.0 33 32939.0 34 48582.0 35 77235.0 36 146307.0 37 334292.0 38 545576.0 39 191806.0 40 229.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8620414414792 19.611518676351714 13.817509409137294 23.7089304730318 2 15.032458110527122 21.21966041380519 40.14207527436835 23.60580620129934 3 17.398601345314546 25.98788797808482 31.760307188409442 24.85320348819119 4 12.07430281578792 18.100849695738948 39.62423140984976 30.200616078623366 5 12.785949190976117 38.19262013755406 36.140866231182514 12.880564440287301 6 29.84222113425269 38.81435027581298 18.339737325207853 13.003691264726482 7 25.61101448502317 33.73357488796983 23.34520151460599 17.31020911240101 8 24.82862892343721 35.292884996751965 21.505792167762362 18.372693912048458 9 25.813199017779414 15.442859691935288 21.053226475251762 37.69071481503354 10-11 23.274875079295835 26.48766604500003 30.593745614519698 19.64371326118444 12-13 24.445500663894247 24.829009924441152 29.585489957766036 21.13999945389856 14-15 21.84926457281215 26.349489680905307 27.25138255739304 24.549863188889503 16-17 21.246425734331808 29.113461463970957 27.908736289520125 21.731376512177107 18-19 21.0631642514378 28.3457444410366 29.817043317909143 20.774047989616452 20-21 21.97413765185271 27.67680192837308 29.687788727323294 20.661271692450907 22-23 21.66731172273786 27.673953207346848 29.363534445835448 21.295200624079843 24-25 21.528305403269567 27.748950419943146 29.400782894805772 21.321961281981512 26-27 21.515190192526155 27.86606417707911 29.319487756850236 21.299257873544498 28-29 21.469501822137303 27.85923790909189 29.461759881737287 21.20950038703352 30-31 21.781605144529557 27.714616278013892 29.44448783622545 21.059290741231106 32-33 21.534081492304733 27.90635503324551 29.291484183060824 21.268079291388933 34-35 21.884951666847645 27.921817323988652 29.14108403675644 21.052146972407275 36-37 21.792019171970516 27.86574667624249 29.115366468990644 21.226867682796343 38-39 21.676226616857136 27.989159251434625 29.123399240157 21.21121489155124 40-41 21.79186042155221 27.811104782261104 29.17727913213051 21.219755664056176 42-43 21.66857484669472 27.874827200169673 29.074853362238613 21.381744590896997 44-45 21.799480441630966 28.124446357916156 28.725189690874835 21.350883509578047 46-47 21.809894469071924 28.002780037325397 28.621843168556747 21.565482325045927 48-49 21.7424255412913 28.173055736001864 28.59720510363545 21.487313619071386 50-51 21.76611110370276 28.259479463728386 28.42432589809874 21.55008353447011 52-53 21.83640578892925 28.375145018507126 28.286498784924298 21.501950407639324 54-55 21.85485258753657 28.295960309855417 28.318852120175336 21.530334982432677 56-57 21.73766302874208 28.40753010384182 28.28656228509162 21.568244582324475 58-59 21.71108820871743 28.31637561364974 28.425468901110555 21.547067276522274 60-61 21.800750444977425 28.24144541620867 28.458552488285804 21.499251650528098 62-63 21.678131621876823 28.263511724353396 28.47026826915689 21.588088384612895 64-65 21.64498453453425 28.28951504287214 28.42191289174047 21.643587530853146 66-67 21.567101579312663 28.26951249016541 28.364349990062227 21.799035940459703 68-69 21.61266294936687 28.25582820410732 28.317042365406632 21.814466481119176 70-71 21.602534672678864 28.092188272916097 28.431977668261155 21.87329938614388 72-73 21.529350729839173 28.10114179650863 28.426294403285752 21.94321307036644 74-75 21.52366746486377 28.164641963831578 28.31288310444698 21.998807466857677 76-77 21.510808680980876 28.140194399412245 28.34917345007204 21.999823469534842 78-79 21.466517314273123 27.998938277202363 28.416039126263097 22.11850528226142 80-81 21.505474666925746 28.148862172251825 28.24522367616439 22.10043948465804 82-83 21.46600931293454 28.143496408113034 28.288594290445957 22.10189998850647 84-85 21.48829787166489 28.07691648267493 28.26328947376776 22.171496171892414 86-87 21.434290979356728 27.975887716464133 28.348792449068107 22.241028855111033 88-89 21.42882996496696 27.96010792488438 28.315740611976516 22.295321498172147 90-91 21.439593243328193 27.773322182703954 28.50811436888136 22.27897020508649 92-93 21.414542427319297 27.721283795582803 28.540372453881417 22.323801323216486 94-95 21.308306647388015 27.67543667477564 28.542372709152087 22.47388396868426 96-97 21.33043645570006 27.630065805223396 28.53227618254774 22.5072215565288 98-99 21.287002341251167 27.506494479612954 28.54484921567768 22.661653963458196 100-101 21.449531019514236 27.418705498288986 28.573583041391316 22.55818044080546 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 382.0 1 248.0 2 191.5 3 278.5 4 242.0 5 247.0 6 375.5 7 702.0 8 1284.5 9 1638.5 10 1468.0 11 1266.0 12 1335.0 13 1484.0 14 1584.0 15 1648.0 16 1785.0 17 1952.5 18 2136.5 19 2420.5 20 2792.0 21 3306.0 22 3975.0 23 4935.5 24 6239.0 25 8012.5 26 10287.0 27 13174.0 28 16263.0 29 19007.5 30 22305.5 31 25903.5 32 29769.0 33 33975.0 34 37750.5 35 40672.5 36 43588.5 37 46915.0 38 49449.5 39 51618.5 40 53712.0 41 55567.0 42 57231.5 43 59098.5 44 60927.0 45 62206.5 46 63047.0 47 63207.0 48 62600.0 49 61242.5 50 58712.0 51 55404.0 52 52081.0 53 48964.5 54 44961.5 55 39827.5 56 34509.0 57 29551.5 58 25918.5 59 22006.0 60 17582.0 61 13957.5 62 10662.0 63 7780.0 64 5625.0 65 4064.5 66 3003.5 67 2252.0 68 1704.5 69 1297.5 70 918.0 71 680.5 72 539.0 73 402.0 74 287.5 75 209.5 76 140.5 77 92.0 78 83.5 79 69.0 80 48.5 81 40.0 82 35.5 83 27.0 84 20.0 85 17.0 86 15.0 87 13.0 88 12.0 89 10.0 90 4.5 91 2.5 92 3.5 93 3.5 94 2.0 95 0.5 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.1750083661470444E-5 24-25 1.5875041830735223E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1574799.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.316118914300326 #Duplication Level Percentage of deduplicated Percentage of total 1 71.62923520318715 44.63655938662197 2 16.400170797096028 20.439899872133434 3 5.891690763586024 11.014419066897348 4 2.6134048215330163 6.5142898251942905 5 1.3400749510718362 4.175413500253387 6 0.7205036148926561 2.6939393366300406 7 0.42303445442470655 1.8453305756743323 8 0.2682647288171318 1.3373773393184731 9 0.17633330036395098 0.9889566222627905 >10 0.5255160539781831 4.862203547283273 >50 0.007984027056504427 0.32689510871708055 >100 0.003480235416202178 0.4564361200602311 >500 0.0 0.0 >1k 3.070485766233467E-4 0.7082796989533259 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4678 0.2970537827367175 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4012 0.25476267129963887 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2415 0.15335290408490226 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.35001673229409E-5 0.0 10-11 0.0 0.0 0.0 6.35001673229409E-5 0.0 12-13 0.0 0.0 0.0 6.35001673229409E-5 1.270003346458818E-4 14-15 0.0 0.0 0.0 6.35001673229409E-5 2.540006692917636E-4 16-17 0.0 0.0 0.0 1.270003346458818E-4 3.1750083661470445E-4 18-19 0.0 0.0 0.0 1.5875041830735223E-4 3.1750083661470445E-4 20-21 0.0 0.0 0.0 1.9050050196882268E-4 3.8100100393764536E-4 22-23 6.35001673229409E-5 0.0 0.0 4.4450117126058626E-4 4.4450117126058626E-4 24-25 6.35001673229409E-5 0.0 0.0 0.001206503179135877 4.4450117126058626E-4 26-27 6.35001673229409E-5 0.0 0.0 0.0018732549360267565 4.4450117126058626E-4 28-29 6.35001673229409E-5 0.0 0.0 0.005842015393710562 4.4450117126058626E-4 30-31 6.35001673229409E-5 0.0 0.0 0.014065287062031409 4.4450117126058626E-4 32-33 6.35001673229409E-5 0.0 0.0 0.026638320191973708 4.4450117126058626E-4 34-35 6.35001673229409E-5 0.0 0.0 0.0464186223130698 4.4450117126058626E-4 36-37 6.35001673229409E-5 0.0 0.0 0.08150246475899464 4.4450117126058626E-4 38-39 6.35001673229409E-5 0.0 0.0 0.13617610882404674 4.4450117126058626E-4 40-41 6.35001673229409E-5 0.0 0.0 0.1950090138487515 4.4450117126058626E-4 42-43 6.35001673229409E-5 0.0 0.0 0.24714265122088597 4.4450117126058626E-4 44-45 6.35001673229409E-5 0.0 0.0 0.3114683207190251 5.397514222449976E-4 46-47 6.35001673229409E-5 0.0 0.0 0.3984635499514541 6.350016732294089E-4 48-49 6.35001673229409E-5 0.0 0.0 0.48717328370160257 7.302519242138203E-4 50-51 6.35001673229409E-5 0.0 0.0 0.5801692787460495 7.620020078752907E-4 52-53 6.35001673229409E-5 0.0 0.0 0.6727525227028973 7.620020078752907E-4 54-55 6.35001673229409E-5 0.0 0.0 0.7735272882444045 7.620020078752907E-4 56-57 6.35001673229409E-5 0.0 0.0 0.9049408845192307 7.937520915367612E-4 58-59 6.35001673229409E-5 0.0 0.0 1.0504197678560883 8.255021751982317E-4 60-61 6.35001673229409E-5 0.0 0.0 1.1833891182303264 8.255021751982317E-4 62-63 6.35001673229409E-5 0.0 0.0 1.309881451537625 8.255021751982317E-4 64-65 6.35001673229409E-5 0.0 0.0 1.476664641011329 8.890023425211725E-4 66-67 6.35001673229409E-5 0.0 0.0 1.679801676277417 8.890023425211725E-4 68-69 6.35001673229409E-5 0.0 0.0 1.927357078585902 8.890023425211725E-4 70-71 6.35001673229409E-5 0.0 0.0 2.1837707542359377 8.890023425211725E-4 72-73 6.35001673229409E-5 0.0 0.0 2.450979458330873 9.207524261826429E-4 74-75 6.35001673229409E-5 0.0 0.0 2.7323169496551625 9.525025098441135E-4 76-77 6.35001673229409E-5 0.0 0.0 3.0278784784597907 9.525025098441135E-4 78-79 6.35001673229409E-5 0.0 0.0 3.365064366944607 0.0010160026771670544 80-81 6.35001673229409E-5 0.0 0.0 3.7351433421027065 0.0010160026771670544 82-83 6.35001673229409E-5 0.0 0.0 4.131289135946874 0.0010160026771670544 84-85 6.35001673229409E-5 0.0 0.0 4.536610703969205 0.0010160026771670544 86-87 6.35001673229409E-5 0.0 0.0 4.966665587163822 0.0011112529281514657 88-89 6.35001673229409E-5 0.0 0.0 5.4631416453782355 0.001206503179135877 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2120 0.0 55.56604 1 GTATCAA 3620 0.0 41.070442 1 TCAACGC 4300 0.0 34.13372 4 ATCAACG 4360 0.0 33.66399 3 CAACGCA 4410 0.0 33.60544 5 AACGCAG 4575 0.0 32.393444 6 TATCAAC 4630 0.0 31.803455 2 ACGCAGA 5070 0.0 28.762327 7 CGCAGAG 5145 0.0 28.620022 8 GTACATG 3195 0.0 26.165884 1 TACATGG 3245 0.0 24.73806 2 GCAGAGT 6040 0.0 24.06457 9 ACATGGG 3405 0.0 22.320116 3 GAGTACT 4325 0.0 22.075146 12-13 AGAGTAC 5290 0.0 21.954157 10-11 CAGAGTA 5845 0.0 21.819931 10-11 CATGGGG 2195 0.0 20.341688 4 AGTACTT 4600 0.0 19.929348 12-13 GTACTTT 4950 0.0 18.80808 14-15 CGGTTCG 90 1.2341855E-4 18.472223 30-31 >>END_MODULE