FastQCFastQC Report
Fri 10 Feb 2017
SRR2031783_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031783_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574799
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49810.3162943334355686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45640.28981476366190223No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT27530.17481596064005628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26560.16865644440973102No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22250.057.1000441
GTATCAA40200.040.599741
TCAACGC47400.034.0708434
CAACGCA48300.033.337645
ATCAACG49150.032.954383
AACGCAG49950.032.4265826
TATCAAC50950.031.8833682
GACCGCG600.003951896431.6658427
ACGCAGA56700.028.4825047
CGCAGAG56150.028.2539398
GCAGAGT66150.023.9109429
AGAGTAC58850.021.79212610-11
CAGAGTA62950.021.50460210-11
GAGTACT48250.021.46057912-13
GTATAAG6100.019.501381
GTACTTT53650.019.3447914-15
GTACATG28850.019.2972411
AGTACTT53250.019.08870512-13
TACATGG29900.018.2687532
TACACCG1300.007565476518.2687535
Produced by FastQC (version 0.11.5)