FastQCFastQC Report
Fri 10 Feb 2017
SRR2031781_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031781_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739387
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79810.29134255218412003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67880.24779266310309567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40640.14835435811004433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41350.061.1124531
GTATCAA74700.041.0776441
ATCAACG85200.035.457753
TCAACGC85100.035.4435924
CAACGCA85800.035.0990685
AACGCAG89600.033.875566
TATCAAC91650.033.0660132
ACGCAGA99700.030.4438327
CGCAGAG100350.030.246648
GCAGAGT118750.025.289
GTACATG70100.024.5292431
CAGAGTA112700.023.60248410-11
GAGTACT81000.023.28086512-13
TACATGG72200.022.434212
ACATGGG70550.021.4776743
GTACTTT87150.021.36546114-15
AGAGTAC105700.021.16603510-11
CATGGGG39650.020.0063064
AGTACTT87350.018.76073312-13
TATCCCG1650.001464512717.2727285
Produced by FastQC (version 0.11.5)