FastQCFastQC Report
Fri 10 Feb 2017
SRR2031781_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031781_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739387
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98350.35902192716837744No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83500.30481271904991886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52790.19270734657060137No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT30300.11060868727200647No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40650.058.7440871
GTATCAA75150.043.612521
TCAACGC92100.035.2247124
ATCAACG92350.035.1293533
CAACGCA93200.034.8599325
AACGCAG95700.033.998916
TATCAAC98000.033.2009772
ACGCAGA107750.029.9762977
CGCAGAG107750.029.9322158
GCAGAGT124600.025.7319139
GTACATG60100.023.9822061
GAGTACT92700.022.44299112-13
AGAGTAC114250.022.40881510-11
CAGAGTA120850.022.40343310-11
TACATGG62950.022.3348122
ACATGGG61400.021.9703183
GTACGGG1656.256883E-520.1806451
AGTACTT101550.019.6919412-13
GTACTTT104650.019.63058514-15
GTATAGC11100.018.42751
Produced by FastQC (version 0.11.5)