FastQCFastQC Report
Fri 10 Feb 2017
SRR2031779_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031779_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17431
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1290.7400608111984395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.5564798347771213No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.5048476851586254No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.4130571969479663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.3901095748953015No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.3384774252768057No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.32126670873730706No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT530.3040559921978085No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG460.26389765360564515No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT440.2524238425793127No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.20652859847398314No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT360.20652859847398314No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG340.19505478744765076No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.16637025988181975No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.13768573231598874No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA230.13194882680282258No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT210.12047501577649017No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA210.12047501577649017No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACC200.11473811026332396No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGTCTCT190.10900120475015776No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACAC190.10900120475015776No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC190.10900120475015776No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT180.10326429923699157No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.10326429923699157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2050.032.4390221
GTATCAA2700.026.3888871
AACGCAG2850.024.9999986
CAACGCA3050.024.9180325
TATCAAC2900.024.5689662
TCAACGC2950.024.1525424
ATCAACG2950.024.1525423
GTACTTT1452.16826E-522.9310343
GAGTACT1452.16826E-522.9310341
TACTTTT1452.16826E-522.9310344
AGTACTT1502.8179786E-522.1666682
ACGCAGA3300.021.590917
CGCAGAG3051.6370905E-1020.2459019
ACTTTTT1655.877633E-520.1515165
AAAAACT800.001159806217.812538-39
CAGAGTA2850.017.510-11
GAGTACA1901.8189894E-1017.512-13
GCAGAGT3006.481605E-817.4166689
GGTCGCC850.001741020416.76470694-95
CTCGGTG1459.609121E-716.3793188-89
Produced by FastQC (version 0.11.5)