##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031779_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17431 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.85084045665768 31.0 15.0 34.0 7.0 34.0 2 24.928460788250817 31.0 15.0 34.0 7.0 34.0 3 24.923297573288966 31.0 15.0 34.0 7.0 34.0 4 27.319029315587173 35.0 17.0 37.0 7.0 37.0 5 27.240720555332455 35.0 17.0 37.0 7.0 37.0 6 27.564167288164764 35.0 17.0 37.0 8.0 37.0 7 27.572198955883195 35.0 17.0 37.0 8.0 37.0 8 27.559405656588837 35.0 17.0 37.0 8.0 37.0 9 28.68521599449257 37.0 17.0 39.0 8.0 39.0 10-11 28.61579943778326 37.0 17.0 39.0 8.0 39.0 12-13 28.62254030176123 37.0 17.0 39.0 8.0 39.0 14-15 29.40126785611841 37.5 17.0 41.0 8.0 41.0 16-17 29.26969192817394 37.5 17.0 41.0 8.0 41.0 18-19 29.30239228959899 37.0 16.5 41.0 8.0 41.0 20-21 29.220584016981242 37.0 16.0 41.0 8.0 41.0 22-23 29.031352188629455 35.5 16.0 41.0 8.0 41.0 24-25 28.836297401181803 35.0 15.0 41.0 8.0 41.0 26-27 28.466266995582583 35.0 14.0 40.5 8.0 41.0 28-29 28.03508117721301 35.0 13.0 40.0 8.0 41.0 30-31 27.67273822500143 35.0 13.0 40.0 7.5 41.0 32-33 27.252481211634446 35.0 13.0 39.5 7.0 41.0 34-35 26.93732430726866 34.0 12.0 39.5 7.0 41.0 36-37 26.47883081865642 33.5 11.5 39.5 7.0 41.0 38-39 25.95617004187941 33.0 9.5 39.0 7.0 41.0 40-41 25.566289943204637 31.5 9.0 39.0 7.0 41.0 42-43 25.006826917560666 30.0 8.0 38.5 7.0 41.0 44-45 24.630772761172622 28.5 8.0 39.0 7.0 41.0 46-47 24.094113934943493 26.5 8.0 39.0 7.0 41.0 48-49 23.68220411909816 24.5 8.0 39.0 7.0 41.0 50-51 22.943491480695315 23.5 8.0 37.5 7.0 40.0 52-53 22.84381274740405 23.0 8.0 37.5 7.0 39.5 54-55 23.09365498250244 23.0 8.0 38.0 7.0 41.0 56-57 22.999770523779475 23.0 8.0 37.5 7.0 41.0 58-59 22.713527623200044 22.0 8.0 37.0 7.0 41.0 60-61 22.488009867477484 22.0 7.5 36.5 7.0 41.0 62-63 22.06803969938615 21.0 7.0 35.5 7.0 40.5 64-65 21.699214043944696 20.0 7.0 35.0 7.0 39.5 66-67 21.313579255349666 19.5 7.0 35.0 7.0 39.0 68-69 20.903304457575583 19.0 7.0 35.0 7.0 37.5 70-71 20.508232459411392 18.0 7.0 35.0 7.0 37.0 72-73 20.073403706040963 17.0 7.0 35.0 7.0 36.0 74-75 19.773679077505594 16.5 7.0 35.0 6.0 35.0 76-77 19.520366014571742 15.0 7.0 34.0 6.0 35.0 78-79 19.296712753140955 15.0 7.0 34.0 5.0 35.0 80-81 19.151884573461075 14.0 7.0 34.0 5.0 35.0 82-83 18.88067236532614 13.0 7.0 34.0 5.0 35.0 84-85 18.629252481211637 12.5 7.0 34.0 5.0 35.0 86-87 18.39403935517182 11.5 7.0 33.0 5.0 35.0 88-89 18.297200390109573 11.0 7.0 33.0 5.0 35.0 90-91 18.097928977109746 11.0 7.0 33.0 5.0 35.0 92-93 17.834174746141933 10.0 7.0 33.0 5.0 35.0 94-95 17.639693649245597 10.0 7.0 32.0 5.0 35.0 96-97 17.490419367793013 10.0 7.0 31.5 5.0 35.0 98-99 17.305404164993405 10.0 7.0 31.0 5.0 35.0 100-101 16.269433767425852 10.0 7.0 28.0 5.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6812646273129168 1101 2 1.9684408737464132 1101 3 2.6367271255092923 1101 4 2.4669312171856816 1101 5 1.6698686808317191 1101 6 1.7859919997931897 1101 7 1.2238406503264478 1101 8 2.2096342394904696 1101 9 1.998148239665749 1101 10-11 2.5043349458027713 1101 12-13 4.474624732328341 1101 14-15 3.530033503572948 1101 16-17 3.804243820856531 1101 18-19 2.6581086789087074 1101 20-21 3.3036306831202644 1101 22-23 4.4029873747082995 1101 24-25 4.703670553691037 1101 26-27 4.7544001267804 1101 28-29 5.188228585093462 1101 30-31 4.002013962521115 1101 32-33 3.9157465592149663 1101 34-35 2.6207260775726056 1101 36-37 4.969221136096003 1101 38-39 5.262731780251556 1101 40-41 6.095817580570454 1101 42-43 7.606705463889593 1101 44-45 4.916072341784563 1101 46-47 5.945590516865071 1101 48-49 4.245163905926216 1101 50-51 7.547676859688732 1101 52-53 7.118989177631356 1101 54-55 6.85603298374091 1101 56-57 7.162960316100328 1101 58-59 8.613268634508447 1101 60-61 7.255686069822115 1101 62-63 7.70087478057431 1101 64-65 7.445680984190915 1101 66-67 6.410239835254263 1101 68-69 8.29538035287247 1101 70-71 6.9074343837161365 1101 72-73 7.634690629843792 1101 74-75 6.689091201849571 1101 76-77 8.993932851780972 1101 78-79 7.494276451544895 1101 80-81 6.154392567504438 1101 82-83 5.597040879109706 1101 84-85 6.808460119321218 1101 86-87 7.2572241374097 1101 88-89 7.746302551711164 1101 90-91 9.37072564522801 1101 92-93 7.12472547787214 1101 94-95 5.075840132456268 1101 96-97 7.551827358916018 1101 98-99 5.340890020244686 1101 100-101 5.82331044519287 1102 1 4.4812646273129175 1102 2 4.901774207079747 1102 3 5.5033937921759595 1102 4 6.400264550519012 1102 5 6.203202014165054 1102 6 6.78599199979319 1102 7 5.090507316993115 1102 8 5.9429675728238 1102 9 5.931481572999086 1102 10-11 6.3710016124694455 1102 12-13 6.674624732328336 1102 14-15 7.463366836906285 1102 16-17 6.904243820856525 1102 18-19 6.191442012242039 1102 20-21 6.603630683120262 1102 22-23 6.369654041374961 1102 24-25 6.9036705536910326 1102 26-27 6.087733460113743 1102 28-29 6.454895251760135 1102 30-31 6.568680629187785 1102 32-33 7.582413225881631 1102 34-35 8.387392744239275 1102 36-37 8.502554469429334 1102 38-39 9.296065113584888 1102 40-41 9.495817580570456 1102 42-43 10.373372130556252 1102 44-45 8.582739008451227 1102 46-47 8.778923850198407 1102 48-49 8.145163905926214 1102 50-51 9.547676859688732 1102 52-53 9.452322510964692 1102 54-55 10.28936631707424 1102 56-57 10.396293649433659 1102 58-59 9.413268634508448 1102 60-61 9.455686069822118 1102 62-63 8.300874780574311 1102 64-65 7.7790143175242505 1102 66-67 7.476906501920933 1102 68-69 7.195380352872469 1102 70-71 5.84076771704947 1102 72-73 4.634690629843792 1102 74-75 6.122424535182908 1102 76-77 5.627266185114308 1102 78-79 5.094276451544896 1102 80-81 5.4543925675044385 1102 82-83 5.397040879109703 1102 84-85 4.941793452654554 1102 86-87 5.057224137409701 1102 88-89 3.612969218377831 1102 90-91 3.604058978561344 1102 92-93 4.05805881120547 1102 94-95 4.47584013245627 1102 96-97 3.2851606922493524 1102 98-99 4.840890020244686 1102 100-101 4.223310445192869 1103 1 3.2229312939795847 1103 2 2.610107540413079 1103 3 2.2950604588426273 1103 4 2.6794312171856802 1103 5 2.8615353474983856 1103 6 0.5901586664598568 1103 7 1.7405073169931136 1103 8 0.5429675728238017 1103 9 1.5981482396657505 1103 10-11 1.7522516124694398 1103 12-13 1.3725413989950077 1103 14-15 2.3842001702396196 1103 16-17 0.8688271541898622 1103 18-19 0.8601920122420417 1103 20-21 2.145297349786933 1103 22-23 0.6925707080416323 1103 24-25 1.2432538870243697 1103 26-27 1.031483460113737 1103 28-29 3.1798952517601293 1103 30-31 3.0311806291877836 1103 32-33 3.307413225881632 1103 34-35 2.062392744239272 1103 36-37 3.64838780276267 1103 38-39 2.3898151135848877 1103 40-41 2.2333175805704535 1103 42-43 2.6796221305562575 1103 44-45 2.7348223417845645 1103 46-47 3.7164238501984066 1103 48-49 1.7139139059262156 1103 50-51 1.3226768596887304 1103 52-53 2.360655844298023 1103 54-55 1.6622829837409085 1103 56-57 0.002543649433661699 1103 58-59 2.029935301175115 1103 60-61 1.5286027364887822 1103 62-63 3.23212478057431 1103 64-65 2.522764317524249 1103 66-67 2.664406501920933 1103 68-69 4.172463686205802 1103 70-71 3.7741010503828036 1103 72-73 3.6721906298437936 1103 74-75 3.522424535182907 1103 76-77 2.7730995184476406 1103 78-79 1.150526451544895 1103 80-81 2.377309234171104 1103 82-83 2.0199575457763714 1103 84-85 1.7980434526545537 1103 86-87 2.575974137409702 1103 88-89 1.3567192183778296 1103 90-91 1.0207256452280085 1103 92-93 0.7747254778721384 1103 94-95 2.611256799122934 1103 96-97 0.4768273589160188 1103 98-99 1.2846400202446873 1103 100-101 1.9566437785262032 1104 1 -1.3187353726870832 1104 2 -0.2982257929202561 1104 3 -0.7632728744907062 1104 4 -2.2664021161476526 1104 5 -0.3967979858349473 1104 6 -0.8140080002068082 1104 7 -2.6428260163402193 1104 8 -1.5903657605095312 1104 9 -1.2685184270009167 1104 10-11 -0.2956650541972259 1104 12-13 -0.02537526767165943 1104 14-15 1.1633668369062882 1104 16-17 1.3709104875231937 1104 18-19 1.8247753455753752 1104 20-21 0.703630683120263 1104 22-23 0.8029873747082981 1104 24-25 0.8370038870243697 1104 26-27 1.5210667934470727 1104 28-29 1.7882285850934636 1104 30-31 1.8353472958544508 1104 32-33 0.4490798925482977 1104 34-35 -0.14594058909406016 1104 36-37 2.135887802762671 1104 38-39 -0.2372682197484437 1104 40-41 1.5291509139037878 1104 42-43 1.2733721305562575 1104 44-45 0.6827390084512324 1104 46-47 -1.4544094831349277 1104 48-49 -0.42150276074045223 1104 50-51 -0.7189898069779339 1104 52-53 -0.5810108223686434 1104 54-55 1.6893663170742421 1104 56-57 -1.1370396838996726 1104 58-59 0.946601967841783 1104 60-61 -0.6443139301778835 1104 62-63 -3.132458552759026 1104 64-65 -6.220985682475751 1104 66-67 -4.089760164745734 1104 68-69 -3.5046196471275337 1104 70-71 -3.125898949617195 1104 72-73 -4.031976036822872 1104 74-75 -2.477575464817093 1104 76-77 -4.039400481552359 1104 78-79 -3.3057235484551057 1104 80-81 -3.278940765828896 1104 82-83 -4.269625787556961 1104 84-85 -4.024873214012114 1104 86-87 -3.5427758625902985 1104 88-89 -3.787030781622171 1104 90-91 -2.0959410214386587 1104 92-93 -2.708607855461196 1104 94-95 -4.190826534210398 1104 96-97 -3.3815059744173155 1104 98-99 -3.892443313088645 1104 100-101 -3.3100228881404625 1105 1 4.847931293979585 1105 2 4.806536111841652 1105 3 4.81291760169977 1105 4 3.0812169314713955 1105 5 6.59367820464124 1105 6 6.652658666459857 1105 7 3.0619358884216865 1105 8 3.8286818585380864 1105 9 3.312433953951466 1105 10-11 3.47100161246944 1105 12-13 5.448434256137869 1105 14-15 4.268128741668189 1105 16-17 3.9090057256184316 1105 18-19 3.4271562979563264 1105 20-21 5.627440206929794 1105 22-23 4.357749279470202 1105 24-25 4.622718172738654 1105 26-27 3.544876317256591 1105 28-29 3.090609537474414 1105 30-31 4.468680629187784 1105 32-33 4.539556083024486 1105 34-35 2.5088213156678414 1105 36-37 3.121602088476955 1105 38-39 4.296065113584888 1105 40-41 5.260103294856169 1105 42-43 4.594800701984827 1105 44-45 4.65892948464171 1105 46-47 3.957495278769837 1105 48-49 0.35230676306907327 1105 50-51 2.6976768596887304 1105 52-53 3.4499415585837383 1105 54-55 1.148890126598051 1105 56-57 2.810579363719377 1105 58-59 2.5656495868894 1105 60-61 7.47949559363164 1105 62-63 5.45087478057431 1105 64-65 3.429014317524249 1105 66-67 2.584049359063787 1105 68-69 2.5072851147772326 1105 70-71 -0.29732752104576576 1105 72-73 0.09183348698665128 1105 74-75 0.3081388208971916 1105 76-77 0.3177423755904982 1105 78-79 1.244276451544895 1105 80-81 0.3639163770282501 1105 82-83 1.3637075457763714 1105 84-85 0.10607916694026898 1105 86-87 -0.3570615768760135 1105 88-89 -2.3084593530507433 1105 90-91 0.48501135951372376 1105 92-93 -0.5109888078421463 1105 94-95 -1.107493200877066 1105 96-97 -1.933886926798266 1105 98-99 -2.130538551183884 1105 100-101 -2.3290705071880815 1106 1 0.46557835280311366 1106 2 1.7056957757071949 1106 3 2.8171192823720403 1106 4 1.2198723936562672 1106 5 1.2071235827925015 1106 6 1.2997174899892698 1106 7 0.2552131993460556 1106 8 1.6606146316473307 1106 9 1.7157952984892795 1106 10-11 0.6768839654106138 1106 12-13 1.9295266931126527 1106 14-15 0.6672884055337356 1106 16-17 -0.16242284581013777 1106 18-19 1.0385008357714547 1106 20-21 0.9761797027281105 1106 22-23 -0.31662046842895464 1106 24-25 -0.2512314070932753 1106 26-27 0.12890993070197254 1106 28-29 1.2901893694071873 1106 30-31 1.0863276880113126 1106 32-33 2.5059426376463385 1106 34-35 1.275628038356917 1106 36-37 0.18882897923325714 1106 38-39 -1.5568760628856992 1106 40-41 -3.0865353606060175 1106 42-43 -3.167804340031978 1106 44-45 -1.6309864817448485 1106 46-47 -0.2798996792133579 1106 48-49 -0.3724831528973134 1106 50-51 -1.1405584344289146 1106 52-53 -0.22022650864315096 1106 54-55 0.8736800425644375 1106 56-57 -0.9625298799781064 1106 58-59 -0.4847705811778269 1106 60-61 -0.17176491057004029 1106 62-63 -0.034419337072748135 1106 64-65 0.23783784693601362 1106 66-67 -0.34662290984377364 1106 68-69 1.416948980323447 1106 70-71 0.5093951680298616 1106 72-73 0.2935141592555581 1106 74-75 0.3459539469476134 1106 76-77 -0.2746945991994174 1106 78-79 -1.09395884257275 1106 80-81 -2.543646648181838 1106 82-83 -0.4598218659883315 1106 84-85 -1.2699712532278014 1106 86-87 -0.4663052743550047 1106 88-89 -1.1635013698574639 1106 90-91 0.03543152758095047 1106 92-93 0.9659019484603739 1106 94-95 -0.6221990832300079 1106 96-97 0.9694744177395478 1106 98-99 -0.47087468563766777 1106 100-101 -0.7786503391208548 1107 1 -3.546805548125679 1107 2 -3.5543661437974485 1107 3 -2.6720448043152665 1107 4 -5.0278056249195835 1107 5 -4.473990968291087 1107 6 -3.189446596698037 1107 7 -3.877913735638465 1107 8 -3.9833482166498833 1107 9 -4.454483339281619 1107 10-11 -3.923735229635824 1107 12-13 -5.027129653636571 1107 14-15 -2.7769840402866954 1107 16-17 -2.8992649510732953 1107 18-19 -3.819084303547431 1107 20-21 -4.334965808107803 1107 22-23 -4.200521397221525 1107 24-25 -4.8208908498177365 1107 26-27 -4.259634960938893 1107 28-29 -2.4977363271872406 1107 30-31 -2.9260562129174765 1107 32-33 -3.0807446688552105 1107 34-35 -2.707344097865988 1107 36-37 -4.032533249868909 1107 38-39 -2.9670927811519547 1107 40-41 -2.546287682587444 1107 42-43 -2.5687331326016363 1107 44-45 -1.8523487108470142 1107 46-47 -1.0105498340121208 1107 48-49 -0.939046620389572 1107 50-51 -0.6839020876796909 1107 52-53 -1.0932915241230319 1107 54-55 -1.0860722794169888 1107 56-57 -0.27212740319791706 1107 58-59 -0.4042752251406725 1107 60-61 0.22761589438352203 1107 62-63 0.7008747805743099 1107 64-65 0.6790143175242491 1107 66-67 -0.4967777086053822 1107 68-69 -0.171286313794198 1107 70-71 -1.0680042127750902 1107 72-73 -0.3442567385772577 1107 74-75 -0.9249438858697232 1107 76-77 0.06586267634237686 1107 78-79 -0.2688814431919475 1107 80-81 -1.3578881342499507 1107 82-83 -1.1099766647499436 1107 84-85 -1.6213644420822888 1107 86-87 -0.45856533627451057 1107 88-89 -1.2765044658326978 1107 90-91 -0.17664277582462162 1107 92-93 -0.264748206338389 1107 94-95 -1.4890721482454872 1107 96-97 -1.96396211476819 1107 98-99 -1.0064784008079428 1107 100-101 -0.4644088530527455 1108 1 -2.494173969178309 1108 2 -2.606997722744815 1108 3 -3.987834277999479 1108 4 -3.5014898354458985 1108 5 -3.1055699156595082 1108 6 -2.399972912487513 1108 7 -3.3515979461647802 1108 8 -4.246506111386726 1108 9 -3.980799128755301 1108 10-11 -4.6342615454252964 1108 12-13 -5.44818228521552 1108 14-15 -3.592773513970908 1108 16-17 -3.8729491615996103 1108 18-19 -3.871715882494801 1108 20-21 -4.38759738705517 1108 22-23 -2.5426266603794225 1108 24-25 -3.5051013761335277 1108 26-27 -4.075424434623105 1108 28-29 -4.129315274555658 1108 30-31 -2.794477265549059 1108 32-33 -3.659692037276262 1108 34-35 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10-11 1.78052542199325 2312 12-13 0.4365294942331026 2312 14-15 0.7205096940491451 2312 16-17 1.9566247732374826 2312 18-19 3.0342991550991805 2312 20-21 -0.18208360259401957 2312 22-23 0.46489213661306295 2312 24-25 1.2417657917862748 2312 26-27 3.2591620315423064 2312 28-29 0.031085727950603825 2312 30-31 1.7782044387115938 2312 32-33 2.3490798925483 2312 34-35 3.1397736966202245 2312 36-37 1.8120782789531447 2312 38-39 1.0817793992991724 2312 40-41 2.081531866284738 2312 42-43 1.4876578448419728 2312 44-45 0.8970247227369441 2312 46-47 1.421780993055549 2312 48-49 3.0784972392595478 2312 50-51 2.376248288260161 2312 52-53 1.9142272728694536 2312 54-55 1.803652031359956 2312 56-57 3.2629603161003295 2312 58-59 2.5656495868894 2312 60-61 1.7652098793459245 2312 62-63 2.8913509710504997 2312 64-65 2.0123476508575813 2312 66-67 2.3578588828733125 2312 68-69 2.233475590967707 2312 70-71 4.012196288478041 2312 72-73 4.163262058415224 2312 74-75 4.308138820897192 2312 76-77 3.127266185114305 2312 78-79 3.2799907372591797 2312 80-81 2.1258211389330093 2312 82-83 3.316088498157324 2312 84-85 2.7370315478926486 2312 86-87 2.666747946933512 2312 88-89 2.8463025517111653 2312 90-91 2.794535169037534 2312 92-93 3.1199635731102333 2312 94-95 2.7139353705515035 2312 96-97 3.0423035493922086 2312 98-99 2.7980328773875414 2312 100-101 0.7899771118595389 2313 1 2.6336455796938694 2313 2 2.330345635651174 2313 3 2.836727125509295 2313 4 1.1050264552809175 2313 5 2.0698686808317177 2313 6 2.081230095031284 2313 7 2.347650174135971 2313 8 2.2096342394904696 2313 9 2.1695768110943234 2313 10-11 2.9471920886599143 2313 12-13 2.0793866370902485 2313 14-15 2.50622397976343 2313 16-17 2.266148582761293 2313 18-19 1.0819182027182315 2313 20-21 1.4845830640726483 2313 22-23 3.179177850898771 2313 24-25 2.5750991251196105 2313 26-27 2.4734477458280217 2313 28-29 1.48346668033156 2313 30-31 1.9210615815687362 2313 32-33 4.1586037020721065 2313 34-35 3.2350117918583194 2313 36-37 2.9073163741912396 2313 38-39 3.3198746373944132 2313 40-41 1.9862937710466433 2313 42-43 2.820991178175305 2313 44-45 2.135119960832185 2313 46-47 2.8503524216269795 2313 48-49 2.768973429735741 2313 50-51 2.900057812069683 2313 52-53 -0.966725108082926 2313 54-55 -1.672538444830522 2313 56-57 1.239150792290804 2313 58-59 2.4466019678417794 2313 60-61 1.5747336888697347 2313 62-63 1.4865890662885946 2313 64-65 1.6790143175242491 2313 66-67 -0.5230934980790671 2313 68-69 0.47157082906294434 2313 70-71 1.5598153360970883 2313 72-73 1.6394525346056987 2313 74-75 0.47480548756385943 2313 76-77 -0.420352862504739 2313 78-79 1.1609431182115593 2313 80-81 2.221059234171104 2313 82-83 1.6732313553001816 2313 84-85 1.6417934526545537 2313 86-87 2.0477003278858916 2313 88-89 3.179635885044494 2313 90-91 1.4850113595137238 2313 92-93 1.2152016683483282 2313 94-95 1.8091734657895984 2313 96-97 -0.10055359346493375 2313 98-99 0.5123185916732567 2313 100-101 -0.5433562214737968 2314 1 1.9034868495351418 2314 2 0.17955198485752177 2314 3 2.7256160143981845 2314 4 2.0891534394079017 2314 5 2.2920909030539427 2314 6 2.3748808886820783 2314 7 -0.1761593496735543 2314 8 -0.12369909384286615 2314 9 -2.4574073158898067 2314 10-11 1.0543349458027755 2314 12-13 -0.7975974898938816 2314 14-15 1.585589059128509 2314 16-17 0.6153549319676408 2314 18-19 1.0858864566864845 2314 20-21 -0.09081376132417773 2314 22-23 0.619654041374968 2314 24-25 -0.4129961129756303 2314 26-27 0.7988445712248478 2314 28-29 -1.08399363712876 2314 30-31 0.6909028514100051 2314 32-33 -0.9286978852294787 2314 34-35 -0.3403850335385066 2314 36-37 1.6414433583182273 2314 38-39 -0.2594904419706694 2314 40-41 0.21248424723711778 2314 42-43 -0.8377389805548532 2314 44-45 -0.5394832137709926 2314 46-47 -1.998853927579372 2314 48-49 -0.8381694274071165 2314 50-51 -1.3856564736446018 2314 52-53 -1.3754552668130877 2314 54-55 -0.24396701625909145 2314 56-57 0.31851587165588313 2314 58-59 -1.025620254380442 2314 60-61 0.28901940315545005 2314 62-63 -0.2713474416479116 2314 64-65 1.7901254286353634 2314 66-67 1.0880176130320436 2314 68-69 -0.14350853601641944 2314 70-71 -1.087010060728307 2314 72-73 -3.181976036822874 2314 74-75 -2.8942421314837574 2314 76-77 -1.9005115926634701 2314 78-79 -1.6446124373439943 2314 80-81 -3.19560743249556 2314 82-83 -3.3307368986680714 2314 84-85 -3.4970954362343356 2314 86-87 -4.142775862590298 2314 88-89 -1.514808559399949 2314 90-91 -0.1737187992164344 2314 92-93 -2.8919411887945294 2314 94-95 -1.7186043119881766 2314 96-97 -1.5370615299728705 2314 98-99 -2.0591099797553127 2314 100-101 -2.1266895548071307 2315 1 -0.015705069656778647 2315 2 2.9169257222312623 2315 3 2.1700604588426273 2315 4 2.685113035367497 2315 5 0.19108080204383882 2315 6 -2.1655231517219597 2315 7 -0.14585631937052312 2315 8 0.22478575464198514 2315 9 0.8708755123930239 2315 10-11 1.1528197942876233 2315 12-13 2.0231095808131876 2315 14-15 1.3785183520577995 2315 16-17 1.4966680632807723 2315 18-19 0.6414420122420417 2315 20-21 -0.34333901384943033 2315 22-23 0.763593435314359 2315 24-25 -0.11754156752108358 2315 26-27 0.02864255102283053 2315 28-29 1.282167979032856 2315 30-31 0.6959533564605103 2315 32-33 0.6369586804270853 2315 34-35 -0.6762436193970913 2315 36-37 0.7449787118535767 2315 38-39 -0.1584803409605655 2315 40-41 1.7503630351159067 2315 42-43 2.887008494192621 2315 44-45 2.288799614511838 2315 46-47 3.1652874865620433 2315 48-49 3.586072996835309 2315 50-51 3.743131405143277 2315 52-53 3.5765649352071165 2315 54-55 4.437851165559088 2315 56-57 3.800839103979115 2315 58-59 3.461753482993295 2315 60-61 2.758716372852419 2315 62-63 3.9963293260288566 2315 64-65 1.7926506811606124 2315 66-67 0.6359974110118429 2315 68-69 2.328713686205802 2315 70-71 2.046828323110077 2315 72-73 2.2119633571165203 2315 74-75 3.0451518079101803 2315 76-77 3.5287813366294607 2315 78-79 3.8806400879085317 2315 80-81 1.652877415989284 2315 82-83 3.0682530003218247 2315 84-85 4.005429816290917 2315 86-87 3.357224137409702 2315 88-89 2.6493328547414663 2315 90-91 2.5889074634098286 2315 92-93 1.8883618415085053 2315 94-95 3.3470522536683873 2315 96-97 4.4881909952796555 2315 98-99 4.622708202062867 2315 100-101 3.25209832398075 2316 1 -0.6920687060204145 2316 2 -1.64489245958692 2316 3 -3.189939541157372 2316 4 -1.4930687828143192 2316 5 -1.6234646525016139 2316 6 -1.187341333540143 2316 7 -2.6294926830068874 2316 8 -0.3370324271761973 2316 9 0.6781482396657488 2316 10-11 -0.6289983875305616 2316 12-13 -2.358708601004995 2316 14-15 -2.703299829760379 2316 16-17 -2.642422845810138 2316 18-19 -0.7085579877579598 2316 20-21 -0.6297026502130656 2316 22-23 -1.283679291958368 2316 24-25 -1.562996112975629 2316 26-27 -1.8722665398862617 2316 28-29 -3.045104748239872 2316 30-31 -1.0713193708122155 2316 32-33 -2.817586774118368 2316 34-35 -1.9926072557607277 2316 36-37 -1.704112197237329 2316 38-39 -1.2239348864151118 2316 40-41 -2.764182419429549 2316 42-43 -2.326627869443744 2316 44-45 -1.383927658215434 2316 46-47 -3.241076149801593 2316 48-49 -4.274836094073784 2316 50-51 -3.072323140311269 2316 52-53 -4.914344155701976 2316 54-55 -3.2839670162590906 2316 56-57 -3.6637063505663363 2316 58-59 -4.420064698824888 2316 60-61 -3.4776472635112192 2316 62-63 -5.95912521942569 2316 64-65 -4.700985682475753 2316 66-67 -3.6030934980790654 2316 68-69 -3.3112863137941986 2316 70-71 -4.285898949617199 2316 72-73 -3.625309370156206 2316 74-75 -2.7975754648170934 2316 76-77 -3.5394004815523594 2316 78-79 -1.5657235484551073 2316 80-81 -2.338940765828898 2316 82-83 -1.9562924542236289 2316 84-85 -2.4582065473454477 2316 86-87 -2.862775862590299 2316 88-89 -2.1070307816221714 2316 90-91 -1.369274354771992 2316 92-93 -2.115274522127862 2316 94-95 -3.097493200877066 2316 96-97 -1.988172641083981 2316 98-99 -1.5791099797553123 2316 100-101 -0.3033562214737948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 21.0 7 480.0 8 1184.0 9 785.0 10 616.0 11 624.0 12 668.0 13 542.0 14 485.0 15 407.0 16 441.0 17 484.0 18 558.0 19 621.0 20 624.0 21 447.0 22 344.0 23 246.0 24 240.0 25 183.0 26 215.0 27 188.0 28 220.0 29 205.0 30 237.0 31 258.0 32 280.0 33 386.0 34 484.0 35 874.0 36 1351.0 37 1749.0 38 913.0 39 70.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.53026217658195 21.6223968791234 12.076186105214846 18.771154839079802 2 21.03149561126728 24.817853249956972 32.31025185015203 21.840399288623715 3 22.328036257242843 29.705696747174574 27.81825483334289 20.148012162239688 4 15.592909184785727 22.930411336125296 30.067121794504043 31.409557684584932 5 14.508634042797317 36.371980953473695 30.021226550398715 19.098158453330274 6 26.73397969135448 33.95100682691756 15.191325798864092 24.123687682863864 7 22.052664792610866 38.01273593023923 20.23406574493718 19.700533532212724 8 26.70529516378865 31.237450519189945 19.87264069760771 22.18461361941369 9 27.198669037920947 18.794102461132464 18.71378578394814 35.29344271699845 10-11 24.92398600195055 27.44822442774367 23.211519706270437 24.41626986403534 12-13 27.548620274224085 28.22844357753428 23.940106706442545 20.282829441799095 14-15 25.3054902185761 28.57265790832425 24.74040502552923 21.38144684757042 16-17 20.452068154437498 32.19264528713212 25.06167173426654 22.293614824163846 18-19 20.988468819918534 30.769892719866903 27.85841317193506 20.3832252882795 20-21 24.353163903390513 28.92834605014055 27.049509494578622 19.66898055189031 22-23 23.002122655039873 30.259308129195112 26.326659399919684 20.411909815845334 24-25 22.442774367506164 30.89610464115656 26.151683781768114 20.50943720956916 26-27 22.47432734782858 29.98680511731972 26.3237909471631 21.2150765876886 28-29 22.746830359703978 29.103321668292125 27.64041076243474 20.50943720956916 30-31 23.816763237909473 29.10619012104871 27.20440594343411 19.87264069760771 32-33 21.27818254833343 30.56049566863634 27.953072112902298 20.208249670127934 34-35 20.486489587516495 30.21341288508978 27.247432734782855 22.052664792610866 36-37 21.51913257988641 30.224886696116116 26.533187998393664 21.72279272560381 38-39 22.94475359990821 28.54397338075842 26.782743388216396 21.728529631116974 40-41 21.484711146807413 28.618553152429584 27.594515518329416 22.302220182433597 42-43 21.576501635018072 28.3259709712581 28.0219149790603 22.07561241466353 44-45 20.89954678446446 27.786701853020478 27.99036199873788 23.323389363777178 46-47 20.684412827720728 27.614594687625495 27.12982617176295 24.571166312890828 48-49 20.847914634845964 27.02369341976938 27.852676266421895 24.27571567896277 50-51 21.177786701853023 25.784521828925477 28.039125695599793 24.99856577362171 52-53 20.893809878951295 25.810337903734727 27.643279215191324 25.652573002122654 54-55 20.265618725259596 25.784521828925477 27.85841317193506 26.09144627387987 56-57 20.69301818599048 25.299753313062933 28.744765073719236 25.262463427227356 58-59 21.516264127129826 25.139119958694277 27.471172049796337 25.873443864379553 60-61 21.73139808387356 26.036945671504792 26.09144627387987 26.140209970741786 62-63 22.164534450117607 25.491939647754002 25.7472319430899 26.596293959038498 64-65 21.659686764958984 25.718547415524068 26.18610521484711 26.43566060466984 66-67 21.501921863346908 25.666915265905573 25.058803281509956 27.772359589237567 68-69 22.838620847914633 25.669783718662153 23.77660489931731 27.714990534105905 70-71 23.174229820434856 24.86088004130572 23.512707245711663 28.45218289254776 72-73 23.423785210257584 24.66582525385807 23.914290631633296 27.99609890425105 74-75 23.77660489931731 24.014686478113706 23.40944294647467 28.799265676094315 76-77 24.086397797028283 24.356032356147097 23.403706040961506 28.153863805863118 78-79 24.531007974298664 23.960185875738627 23.6762090528369 27.83259709712581 80-81 26.31231713613677 24.0835293442717 22.609144627387987 26.995008892203547 82-83 27.020824967012793 24.2871894899891 22.689461304572315 26.002524238425796 84-85 26.70816361654523 23.504101887441912 23.105386954276863 26.68234754173599 86-87 26.80569101026906 24.00321266708737 22.571854741552407 26.619241581091156 88-89 27.304801789914517 22.72961964316448 23.504101887441912 26.46147667947909 90-91 28.254259652343528 22.38827376513109 23.266020308645516 26.09144627387987 92-93 28.936951408410305 22.241982674545348 23.363547702369342 25.457518214675005 94-95 28.016178073547128 23.131203029086112 23.845447765475303 25.00717113189146 96-97 28.18828523894211 22.612013080144568 24.224083529344274 24.975618151569044 98-99 27.244564282026275 22.921805977855545 24.215478171074523 25.618151569043658 100-101 29.547931845562502 22.54890711949974 23.182835178704604 24.720325856233146 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 98.0 1 57.0 2 19.5 3 22.5 4 16.0 5 13.5 6 20.0 7 30.0 8 34.5 9 30.0 10 27.5 11 23.5 12 17.5 13 13.0 14 13.5 15 18.5 16 19.0 17 21.0 18 28.5 19 32.0 20 32.0 21 32.5 22 42.5 23 52.0 24 53.0 25 62.5 26 84.5 27 104.5 28 129.5 29 176.0 30 201.5 31 214.5 32 262.5 33 281.5 34 298.5 35 334.0 36 344.5 37 348.0 38 338.0 39 315.0 40 286.0 41 272.5 42 286.5 43 297.0 44 302.5 45 325.0 46 337.5 47 350.0 48 413.0 49 537.0 50 710.0 51 881.0 52 984.5 53 894.5 54 692.0 55 586.5 56 554.5 57 522.0 58 496.5 59 473.5 60 418.5 61 364.5 62 324.5 63 271.0 64 225.5 65 194.0 66 164.5 67 144.0 68 127.5 69 111.0 70 93.0 71 81.5 72 77.0 73 68.5 74 57.0 75 49.0 76 43.0 77 36.5 78 30.0 79 31.0 80 26.0 81 14.0 82 12.0 83 13.0 84 9.0 85 8.5 86 6.0 87 3.5 88 5.0 89 4.0 90 1.5 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 17431.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.52360736618668 #Duplication Level Percentage of deduplicated Percentage of total 1 93.461089360533 78.06207331765246 2 3.9288412665705064 6.56301990706213 3 1.140188199738993 2.8569789455567665 4 0.453327838450443 1.5145430554758763 5 0.30908716257984753 1.2908037404623947 6 0.192320901160794 0.9638001262119213 7 0.082423243354626 0.4819000631059607 8 0.10989765780616802 0.7343239056852734 9 0.041211621677313 0.30979289771097474 >10 0.2266639192252215 3.654408811886868 >50 0.0480802252901985 2.8282944179909357 >100 0.006868603612885501 0.7400608111984395 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 129 0.7400608111984395 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 97 0.5564798347771213 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 88 0.5048476851586254 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 72 0.4130571969479663 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68 0.3901095748953015 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 59 0.3384774252768057 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56 0.32126670873730706 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 53 0.3040559921978085 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 46 0.26389765360564515 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 44 0.2524238425793127 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36 0.20652859847398314 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT 36 0.20652859847398314 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 34 0.19505478744765076 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29 0.16637025988181975 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 24 0.13768573231598874 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA 23 0.13194882680282258 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT 21 0.12047501577649017 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA 21 0.12047501577649017 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACC 20 0.11473811026332396 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTACCTGTCTCT 19 0.10900120475015776 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACAC 19 0.10900120475015776 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC 19 0.10900120475015776 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18 0.10326429923699157 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.10326429923699157 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.017210716539498596 0.0 22-23 0.0 0.0 0.0 0.08031667718432678 0.0 24-25 0.0 0.0 0.0 0.21800240950031552 0.0 26-27 0.0 0.0 0.0 0.4130571969479663 0.0 28-29 0.0 0.0 0.0 0.9121679765934254 0.0 30-31 0.0 0.0 0.0 1.5690436578509552 0.0 32-33 0.005736905513166198 0.0 0.0 2.2976306580230625 0.0 34-35 0.005736905513166198 0.0 0.0 3.38764270552464 0.0 36-37 0.005736905513166198 0.0 0.0 4.641156560151455 0.0 38-39 0.005736905513166198 0.0 0.0 5.897538867534852 0.0 40-41 0.005736905513166198 0.0 0.0 7.882508175090356 0.0 42-43 0.005736905513166198 0.0 0.0 8.932361883999771 0.0 44-45 0.005736905513166198 0.0 0.0 9.463025643967644 0.0 46-47 0.005736905513166198 0.0 0.0 9.933451896047274 0.0 48-49 0.005736905513166198 0.0 0.0 10.46698410877173 0.0 50-51 0.005736905513166198 0.0 0.0 11.009121679765933 0.0 52-53 0.005736905513166198 0.0 0.0 11.267282427858413 0.0 54-55 0.005736905513166198 0.0 0.0 11.840972979175032 0.0 56-57 0.005736905513166198 0.0 0.0 12.497848660432563 0.0 58-59 0.005736905513166198 0.0 0.0 12.833457632952785 0.0 60-61 0.005736905513166198 0.0 0.0 13.025643967643854 0.0 62-63 0.005736905513166198 0.0 0.0 13.177671963742757 0.0 64-65 0.005736905513166198 0.0 0.0 13.289541621249498 0.0 66-67 0.005736905513166198 0.0 0.0 13.470254144914232 0.0 68-69 0.005736905513166198 0.0 0.0 13.739888704033046 0.0 70-71 0.005736905513166198 0.0 0.0 13.94928575526361 0.0 72-73 0.005736905513166198 0.0 0.0 14.112787562388847 0.0 74-75 0.005736905513166198 0.0 0.0 14.307842349836498 0.0 76-77 0.005736905513166198 0.0 0.0 14.451264987665652 0.0 78-79 0.005736905513166198 0.0 0.0 14.600424531007974 0.0 80-81 0.005736905513166198 0.0 0.0 14.743847168837128 0.0 82-83 0.005736905513166198 0.0 0.0 14.93890195628478 0.0 84-85 0.005736905513166198 0.0 0.0 15.111009121679766 0.0 86-87 0.005736905513166198 0.0 0.0 15.291721645344502 0.0 88-89 0.005736905513166198 0.0 0.0 15.558487751706728 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 205 0.0 32.439022 1 GTATCAA 270 0.0 26.388887 1 AACGCAG 285 0.0 24.999998 6 CAACGCA 305 0.0 24.918032 5 TATCAAC 290 0.0 24.568966 2 TCAACGC 295 0.0 24.152542 4 ATCAACG 295 0.0 24.152542 3 GTACTTT 145 2.16826E-5 22.931034 3 GAGTACT 145 2.16826E-5 22.931034 1 TACTTTT 145 2.16826E-5 22.931034 4 AGTACTT 150 2.8179786E-5 22.166668 2 ACGCAGA 330 0.0 21.59091 7 CGCAGAG 305 1.6370905E-10 20.245901 9 ACTTTTT 165 5.877633E-5 20.151516 5 AAAAACT 80 0.0011598062 17.8125 38-39 CAGAGTA 285 0.0 17.5 10-11 GAGTACA 190 1.8189894E-10 17.5 12-13 GCAGAGT 300 6.481605E-8 17.416668 9 GGTCGCC 85 0.0017410204 16.764706 94-95 CTCGGTG 145 9.609121E-7 16.37931 88-89 >>END_MODULE