FastQCFastQC Report
Fri 10 Feb 2017
SRR2031779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17431
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT270515.518329413114564No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT10566.058172221903505RNA PCR Primer, Index 33 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT10445.989329355745511RNA PCR Primer, Index 33 (95% over 22bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG7074.055992197808502RNA PCR Primer, Index 33 (96% over 29bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT4282.4553955596351327RNA PCR Primer, Index 33 (96% over 27bp)
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG2381.3653835121335551RNA PCR Primer, Index 15 (95% over 24bp)
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC1530.8777465435144284RNA PCR Primer, Index 15 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.7457977167116058No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA1170.6712179450404452RNA PCR Primer, Index 33 (96% over 29bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.665481039527279No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.6195857954219495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.5909012678561184No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.5163214961849578No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC840.4819000631059607No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT730.41879410246113247No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT620.35568814181630426RNA PCR Primer, Index 33 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.3442143307899719No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC580.3327405197636395No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.2237393150134817No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT390.2237393150134817No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG380.21800240950031552No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.21226550398714936No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT370.21226550398714936No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGG340.19505478744765076No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACAAGAGGC320.18358097642131835No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT290.16637025988181975No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTCTTCT290.16637025988181975Illumina PCR Primer Index 4 (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG290.16637025988181975No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT270.15489644885548737RNA PCR Primer, Index 15 (96% over 27bp)
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.14915954334232115No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.14915954334232115No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA250.14342263782915496No Hit
GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA220.12621192128965636No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA220.12621192128965636No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT220.12621192128965636No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC210.12047501577649017No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC200.11473811026332396No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT200.11473811026332396No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTC190.10900120475015776No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCA255.1306553E-576.000011
ACCCATG255.1306553E-576.000013
TACCCAT255.1306553E-576.000012
CGCAGAA504.0029408E-757.0000049
TATACAC5600.044.955363
TTATACA5500.044.9090922
CTTATAC5400.044.861111
ATACACA6300.039.206354
TACACAT6400.038.593755
GGTATCA2600.032.8846171
ACACATC7900.031.2658216
CACATCT7950.031.0691817
ACATCTC8150.030.8895688
CATCTCC8700.028.936789
ACACCCC350.00828217427.14285978-79
CATGGGA705.538968E-727.14285916-17
GTATCAA3300.025.9090922
AACGCAG3300.024.4696987
TATCAAC3350.024.1044793
ATCAACG3350.024.1044794
Produced by FastQC (version 0.11.5)