##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031779_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17431 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.40904136308875 34.0 31.0 34.0 30.0 34.0 2 32.56686363375595 34.0 31.0 34.0 31.0 34.0 3 32.51643623429522 34.0 31.0 34.0 30.0 34.0 4 36.054443233319944 37.0 35.0 37.0 35.0 37.0 5 36.074350295450635 37.0 35.0 37.0 35.0 37.0 6 36.17331191555275 37.0 37.0 37.0 35.0 37.0 7 36.08484883253973 37.0 36.0 37.0 35.0 37.0 8 36.15351959153233 37.0 37.0 37.0 35.0 37.0 9 37.82892547759739 39.0 38.0 39.0 35.0 39.0 10-11 37.84481670586885 39.0 38.0 39.0 35.0 39.0 12-13 37.807268659285185 39.0 38.0 39.0 35.0 39.0 14-15 39.19995984166141 41.0 39.0 41.0 36.0 41.0 16-17 38.99979920830704 41.0 38.5 41.0 35.5 41.0 18-19 39.10897252022259 41.0 39.0 41.0 35.5 41.0 20-21 39.18452756583099 41.0 39.0 41.0 35.0 41.0 22-23 38.95582582754862 41.0 38.0 41.0 35.0 41.0 24-25 38.71619528426367 40.5 38.0 41.0 35.0 41.0 26-27 38.335695026102925 40.0 37.5 41.0 34.0 41.0 28-29 37.90032126670874 40.0 36.0 41.0 33.0 41.0 30-31 37.520738913430094 39.5 35.5 41.0 33.0 41.0 32-33 36.507457977167114 38.5 35.0 40.5 30.5 41.0 34-35 36.19950088922036 38.0 35.0 40.5 30.0 41.0 36-37 36.10266192415811 39.0 35.0 41.0 30.0 41.0 38-39 34.79811829499168 38.0 34.5 40.0 23.5 41.0 40-41 34.93112844931444 38.0 35.0 40.0 23.5 41.0 42-43 34.74189662096265 38.0 34.5 40.0 21.5 41.0 44-45 34.127187195226895 38.0 33.5 40.0 16.0 41.0 46-47 33.6933337157937 38.0 33.0 40.0 11.0 41.0 48-49 33.66393207503872 38.5 33.0 40.5 10.0 41.0 50-51 32.871980953473695 38.0 32.0 40.0 8.0 41.0 52-53 32.97710974700247 38.0 33.0 39.0 8.0 41.0 54-55 32.80219149790603 38.0 33.0 39.0 8.0 41.0 56-57 31.932964259078652 36.5 31.5 38.5 8.0 41.0 58-59 31.137427571567898 35.0 29.5 37.0 7.0 41.0 60-61 29.80741208192301 35.0 24.0 37.0 7.0 41.0 62-63 28.235299179622512 34.5 19.0 36.5 7.0 40.0 64-65 26.81415294590098 33.5 14.5 36.0 7.0 39.0 66-67 24.997332338936378 31.5 10.0 35.0 7.0 39.0 68-69 23.035941713039986 25.5 8.0 35.0 7.0 37.0 70-71 21.4142906316333 21.5 7.5 35.0 6.0 37.0 72-73 20.253571223681945 17.0 7.0 35.0 6.0 36.0 74-75 20.177815386380587 17.0 7.0 35.0 7.0 35.0 76-77 19.77887097699501 18.0 7.0 33.5 7.0 35.0 78-79 20.342722735356546 19.0 7.0 34.5 7.0 35.0 80-81 20.380987895129365 19.0 7.0 35.0 7.0 35.0 82-83 20.340886925592336 19.0 7.0 35.0 7.0 35.0 84-85 20.07698927198669 18.5 7.0 35.0 7.0 35.0 86-87 19.960214560266195 18.0 7.0 35.0 7.0 35.0 88-89 19.816935345074867 17.5 7.0 35.0 7.0 35.0 90-91 19.74757615742069 17.0 7.0 35.0 7.0 35.0 92-93 19.730652286156847 17.0 7.0 34.5 7.0 35.0 94-95 19.66416155125925 17.0 7.0 34.0 7.0 35.0 96-97 19.56680626470082 17.0 7.0 34.0 7.0 35.0 98-99 19.478343181687798 17.0 7.0 34.0 7.0 35.0 100-101 18.633985428259997 13.5 7.0 32.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.03980554451086249 1101 2 0.1357382348597369 1101 3 -0.12888839389075457 1101 4 0.12588030354007174 1101 5 0.5480317668596015 1101 6 -0.009228527599233871 1101 7 -0.44899343165779726 1101 8 -0.23040905249288102 1101 9 0.048637528292871934 1101 10-11 -0.9270145657775046 1101 12-13 -0.7077375144425133 1101 14-15 -0.1945247431080972 1101 16-17 0.43787480365192977 1101 18-19 0.1534039204594535 1101 20-21 0.8961009257765511 1101 22-23 0.937423551943148 1101 24-25 0.6449048430741513 1101 26-27 -0.3346261074688144 1101 28-29 1.0502498957058322 1101 30-31 0.8853084759245959 1101 32-33 0.25309274712845564 1101 34-35 0.3792594830144438 1101 36-37 -0.34200823826446225 1101 38-39 -0.2994733059769885 1101 40-41 0.7472270236946486 1101 42-43 -1.2657576056612996 1101 44-45 0.4032134428483971 1101 46-47 0.525763309806905 1101 48-49 0.9266079484407825 1101 50-51 2.370412364924441 1101 52-53 2.014361400537794 1101 54-55 2.341694385892829 1101 56-57 2.445455246725583 1101 58-59 2.9301541226673216 1101 60-61 2.6604695578628004 1101 62-63 2.8939888167552006 1101 64-65 3.410087196401406 1101 66-67 3.832134375751906 1101 68-69 2.8474875521137086 1101 70-71 4.515139517168944 1101 72-73 5.901609394113283 1101 74-75 4.850544556289588 1101 76-77 6.1086659805431935 1101 78-79 6.347682852827656 1101 80-81 4.907097685621228 1101 82-83 6.49043873757304 1101 84-85 6.584266886145539 1101 86-87 6.870017478490681 1101 88-89 6.930517961366892 1101 90-91 5.839737648503871 1101 92-93 6.5876219706842996 1101 94-95 4.976640607530175 1101 96-97 5.0471435031820455 1101 98-99 5.4655941686134994 1101 100-101 6.0320218586556145 1102 1 0.8268611221558047 1102 2 0.8024049015264012 1102 3 0.8044449394425754 1102 4 0.3258803035400746 1102 5 0.21469843352626583 1102 6 0.3907714724007647 1102 7 0.3510065683421999 1102 8 0.23625761417378754 1102 9 0.5153041949595405 1102 10-11 0.20631876755582113 1102 12-13 0.2255958188908167 1102 14-15 0.3054752568919028 1102 16-17 0.9045414703185912 1102 18-19 0.1534039204594535 1102 20-21 -0.1705657408901189 1102 22-23 0.0374235519431565 1102 24-25 0.21157150974081418 1102 26-27 0.19870722586451706 1102 28-29 1.0835832290391707 1102 30-31 -1.2146915240754055 1102 32-33 -1.580240586204873 1102 34-35 0.07925948301443952 1102 36-37 1.2579917617355463 1102 38-39 0.7338600273563429 1102 40-41 1.0805603570279843 1102 42-43 1.6675757276720233 1102 44-45 2.1365467761817314 1102 46-47 2.6924299764735693 1102 48-49 1.4932746151074454 1102 50-51 1.5037456982577737 1102 52-53 1.2810280672044598 1102 54-55 1.0083610525594935 1102 56-57 1.1787885800589173 1102 58-59 1.2968207893339887 1102 60-61 3.060469557862799 1102 62-63 4.660655483421863 1102 64-65 5.043420529734739 1102 66-67 3.3321343757519095 1102 68-69 5.280820885447042 1102 70-71 4.781806183835609 1102 72-73 3.76827606077995 1102 74-75 5.617211222956257 1102 76-77 5.875332647209859 1102 78-79 4.481016186160989 1102 80-81 3.8737643522878926 1102 82-83 4.390438737573042 1102 84-85 3.950933552812206 1102 86-87 4.703350811824013 1102 88-89 4.59718462803356 1102 90-91 5.006404315170535 1102 92-93 5.5876219706842996 1102 94-95 4.809973940863504 1102 96-97 4.447143503182044 1102 98-99 4.398927501946833 1102 100-101 6.165355191988951 1103 1 -0.0023055445108610684 1103 2 0.4649049015264026 1103 3 -0.15805506055742313 1103 4 0.10921363687340602 1103 5 0.1980317668596001 1103 6 0.2282714724007633 1103 7 0.3885065683422013 1103 8 -0.10124238582621103 1103 9 0.8069708616262048 1103 10-11 0.42090210088915825 1103 12-13 0.4401791522241538 1103 14-15 -0.8174414097747658 1103 16-17 -0.08920852968140736 1103 18-19 0.6034039204594563 1103 20-21 0.04818425910988111 1103 22-23 -0.27299311472351206 1103 24-25 0.19073817640748558 1103 26-27 0.7299572258645171 1103 28-29 0.24399989570583358 1103 30-31 0.5582251425912617 1103 32-33 0.3301760804617899 1103 34-35 -0.008240516985559054 1103 36-37 0.2329917617355406 1103 38-39 -1.02030663931032 1103 40-41 0.6034770236946514 1103 42-43 -1.057424272327971 1103 44-45 0.5719634428484 1103 46-47 2.002846643140238 1103 48-49 -0.47339205155922315 1103 50-51 -0.5691709684088906 1103 52-53 -0.2210552661288716 1103 54-55 -0.5541389474405065 1103 56-57 1.2621219133922459 1103 58-59 0.8301541226673201 1103 60-61 0.5438028911961332 1103 62-63 1.4210721500885342 1103 64-65 2.5955038630680747 1103 66-67 3.9446343757519067 1103 68-69 2.3870708854470415 1103 70-71 3.48805618383561 1103 72-73 5.005776060779947 1103 74-75 4.544294556289589 1103 76-77 3.3815826472098607 1103 78-79 3.6622661861609878 1103 80-81 2.9862643522878933 1103 82-83 2.2779387375730415 1103 84-85 2.0467668861455373 1103 86-87 1.780434145157347 1103 88-89 2.0909346280335583 1103 90-91 3.1689043151705363 1103 92-93 2.804288637350968 1103 94-95 2.1349739408635067 1103 96-97 2.4617268365153784 1103 98-99 1.8635108352801666 1103 100-101 2.8236885253222823 1104 1 0.8935277888224746 1104 2 0.9357382348597341 1104 3 0.7377782727759126 1104 4 0.12588030354007174 1104 5 0.6813651001929344 1104 6 0.5241048057340976 1104 7 0.3510065683421999 1104 8 0.5695909475071232 1104 9 0.7819708616262062 1104 10-11 0.7063187675558211 1104 12-13 0.25892915222414814 1104 14-15 0.5054752568919056 1104 16-17 0.3045414703185898 1104 18-19 0.3200705871261249 1104 20-21 -0.37056574089012173 1104 22-23 -0.22924311472350922 1104 24-25 -0.6550951569258459 1104 26-27 0.26537389253117993 1104 28-29 0.8835832290391679 1104 30-31 -0.4813581907420712 1104 32-33 -0.5469072528715415 1104 34-35 -3.8540738503188905 1104 36-37 -4.408674904931125 1104 38-39 -2.33280663931032 1104 40-41 -1.9194396429720157 1104 42-43 -1.865757605661308 1104 44-45 -1.5634532238182715 1104 46-47 -3.340903356859762 1104 48-49 -3.5400587182258896 1104 50-51 -3.229587635075557 1104 52-53 -2.452305266128871 1104 54-55 -4.524972280773838 1104 56-57 -3.2878780866077513 1104 58-59 -3.2031792106660113 1104 60-61 -2.072863775470534 1104 62-63 -4.172677849911466 1104 64-65 -4.689912803598592 1104 66-67 -5.001198957581426 1104 68-69 -5.585845781219625 1104 70-71 -1.9181938161643899 1104 72-73 -3.965057272553384 1104 74-75 -5.482788777043744 1104 76-77 -4.658000686123472 1104 78-79 -4.485650480505679 1104 80-81 -5.092902314378772 1104 82-83 -5.176227929093624 1104 84-85 -5.349066447187797 1104 86-87 -3.8633158548426536 1104 88-89 -3.036148705299773 1104 90-91 -3.126929018162798 1104 92-93 -3.8790446959823655 1104 94-95 -5.190026059136493 1104 96-97 -5.252856496817955 1104 98-99 -5.067739164719832 1104 100-101 -4.301311474677718 1105 1 0.34590874120342363 1105 2 -0.24045224133074328 1105 3 -0.38126934627170783 1105 4 0.0020707797305519193 1105 5 -0.3376825188546846 1105 6 0.3622000438293327 1105 7 -0.3347077173720834 1105 8 -0.09231381439764164 1105 9 0.4676851473404895 1105 10-11 0.46108067231772765 1105 12-13 -0.16249941920441557 1105 14-15 -0.48261998120333516 1105 16-17 -0.20974424396712266 1105 18-19 -0.7894532223976896 1105 20-21 -0.4562800266044036 1105 22-23 -0.17924311472351206 1105 24-25 -0.5860475378782297 1105 26-27 -0.015578488421198244 1105 28-29 0.007392752848687678 1105 30-31 0.6876894283055535 1105 32-33 1.7721403661760817 1105 34-35 0.03640234015730215 1105 36-37 -1.07057966683589 1105 38-39 -0.22566378216745875 1105 40-41 0.3400841665517902 1105 42-43 0.21043287052916781 1105 44-45 0.24607058570553875 1105 46-47 -0.8051890711454703 1105 48-49 -1.2948206229877925 1105 50-51 -0.8414923969803212 1105 52-53 -2.8594481232717293 1105 54-55 -2.0987818045833606 1105 56-57 -1.3093066580363235 1105 58-59 -0.41984587733267986 1105 60-61 -1.3133399659467209 1105 62-63 -1.1726778499114658 1105 64-65 -1.5161032797890712 1105 66-67 -1.3678656242480933 1105 68-69 -0.41203625741010086 1105 70-71 1.0460918981213254 1105 72-73 2.0682760607799473 1105 74-75 2.307687413432447 1105 76-77 2.082475504352715 1105 78-79 3.1310161861609878 1105 80-81 3.209478638002178 1105 82-83 1.5904387375730415 1105 84-85 2.2342668861455373 1105 86-87 2.936684145157347 1105 88-89 4.461470342319274 1105 90-91 1.892118600884821 1105 92-93 3.840002923065253 1105 94-95 4.58140251229208 1105 96-97 2.609048265086809 1105 98-99 2.760832263851597 1105 100-101 2.05583138246514 1106 1 0.5601944554891389 1106 2 0.5788754897616997 1106 3 0.40444493944257687 1106 4 0.27097834275576105 1106 5 0.6539141198007741 1106 6 0.14371264887135027 1106 7 0.3039477448127883 1106 8 0.600963496526731 1106 9 0.6525590969203208 1106 10-11 0.37494621853621624 1106 12-13 0.7765762110476828 1106 14-15 -0.10236788036299771 1106 16-17 0.5339532350244767 1106 18-19 -0.17600784424642768 1106 20-21 1.035316612051055 1106 22-23 1.041345120570604 1106 24-25 0.6429440587604276 1106 26-27 0.6104719317468721 1106 28-29 1.2678969545293626 1106 30-31 -0.053907210349912305 1106 32-33 1.751131962814732 1106 34-35 2.149847718308557 1106 36-37 2.0050505852649536 1106 38-39 1.34366394892497 1106 40-41 1.4178152589887674 1106 42-43 -0.5500713311515 1106 44-45 -0.24972773362218703 1106 46-47 -0.8703151215656462 1106 48-49 -3.002803816265107 1106 50-51 -2.6923327331147746 1106 52-53 -2.693481736717107 1106 54-55 -3.315168359205213 1106 56-57 -2.370231027784225 1106 58-59 -0.8757282302738574 1106 60-61 0.44086171472554625 1106 62-63 -0.29032490873499484 1106 64-65 0.8473420983621907 1106 66-67 2.1027226110460226 1106 68-69 2.7161150030940995 1106 70-71 1.787688536776784 1106 72-73 1.8623937078387698 1106 74-75 1.4505445562895893 1106 76-77 0.6223914707392737 1106 78-79 1.8516044214551037 1106 80-81 2.614940822876129 1106 82-83 1.149262266984806 1106 84-85 1.4842668861455373 1106 86-87 -0.1221393842544174 1106 88-89 -1.5322271366723257 1106 90-91 -1.3347721554176992 1106 92-93 -1.4457113626490319 1106 94-95 -0.5488495885482614 1106 96-97 -1.2606996340728571 1106 98-99 -1.7853862235433624 1106 100-101 -0.6542526511483047 1107 1 0.8759839291733513 1107 2 0.8234575331053477 1107 3 0.8781291499688919 1107 4 0.4381610052944609 1107 5 0.7375054510701275 1107 6 0.001297788190235849 1107 7 0.4773223578158863 1107 8 0.3625734036474739 1107 9 0.33986559846830744 1107 10-11 0.4291257850996857 1107 12-13 0.26419231011889366 1107 14-15 0.3124928007515493 1107 16-17 0.5571730492659626 1107 18-19 0.8270881309857714 1107 20-21 1.2504868906888262 1107 22-23 0.9918095168554331 1107 24-25 0.9045539658811705 1107 26-27 1.4618651206013595 1107 28-29 1.3344604220216283 1107 30-31 1.5203961952228369 1107 32-33 1.38116292256705 1107 34-35 2.705575272488126 1107 36-37 2.700097024893431 1107 38-39 3.27245651858442 1107 40-41 3.4735428131683364 1107 42-43 4.3044178329351865 1107 44-45 3.2611081796905097 1107 46-47 3.132780853666553 1107 48-49 3.552923737914462 1107 50-51 4.073921136854274 1107 52-53 3.537168418081656 1107 54-55 2.6136242104542333 1107 56-57 2.880542966023821 1107 58-59 2.38278570161469 1107 60-61 -0.31146026669860305 1107 62-63 -1.3568883762272534 1107 64-65 -1.5442987685108704 1107 66-67 -0.288918255827042 1107 68-69 1.770294569657569 1107 70-71 -0.12872013195386245 1107 72-73 -0.9054081497463677 1107 74-75 -1.654718601605147 1107 76-77 -0.6825620896322455 1107 78-79 -1.0137206559442724 1107 80-81 -0.8525514371857916 1107 82-83 -1.5148244203216947 1107 84-85 -1.1999436401702503 1107 86-87 -0.14226322326370777 1107 88-89 0.0235004175072433 1107 90-91 -0.13043779009262124 1107 92-93 -0.39307978370166197 1107 94-95 -0.09528921703122961 1107 96-97 0.864687362831166 1107 98-99 0.06910294054332411 1107 100-101 0.17237273584859736 1108 1 0.1391418239101938 1108 2 0.3497733225790327 1108 3 0.35181336049520695 1108 4 0.1750031105576184 1108 5 0.3164528194911824 1108 6 0.001297788190235849 1108 7 -0.25951974744727124 1108 8 -0.5321634384577933 1108 9 -0.5548712436369527 1108 10-11 -0.018242635952944397 1108 12-13 0.2905080995925786 1108 14-15 0.2861770112778643 1108 16-17 -0.5480901086287773 1108 18-19 0.3270881309857714 1108 20-21 0.11890794332040855 1108 22-23 -0.2713483778814094 1108 24-25 -1.9638670867504047 1108 26-27 -1.3539243530828529 1108 28-29 -0.6129079990310018 1108 30-31 -0.08486696267189586 1108 32-33 0.0653734488828448 1108 34-35 -1.373372095932929 1108 36-37 -3.273587185632884 1108 38-39 -2.6485961129945323 1108 40-41 -2.0527729763053486 1108 42-43 -1.5376874302227108 1108 44-45 -2.94941813609897 1108 46-47 -1.8145875673860772 1108 48-49 -1.2102341568223807 1108 50-51 -0.2944999157773154 1108 52-53 0.14243157597638856 1108 54-55 -0.17584947375630122 1108 56-57 0.09106928181330076 1108 58-59 -1.11721429838531 1108 60-61 -0.4430392140670243 1108 62-63 0.19574320272011292 1108 64-65 -0.517982979037189 1108 66-67 0.8952922704887492 1108 68-69 0.45450509597335653 1108 70-71 -2.444509605638075 1108 72-73 -1.5106713076411076 1108 74-75 -2.4178764963419894 1108 76-77 -4.366772615948033 1108 78-79 -2.171615392786382 1108 80-81 0.12113277334052341 1108 82-83 -2.646403367690116 1108 84-85 -3.831522587538675 1108 86-87 -3.694894802211074 1108 88-89 -2.5028153719664417 1108 90-91 -3.3672798953557788 1108 92-93 -3.6562376784385044 1108 94-95 -2.4637102696628084 1108 96-97 -2.582681058221464 1108 98-99 -1.2730023226145697 1108 100-101 -1.7749956852040327 1109 1 0.736665043724436 1109 2 0.8141696074087577 1109 3 0.9338567041484609 1109 4 0.506272460402819 1109 5 0.7127376492125421 1109 6 0.6731244135772343 1109 7 0.7157124506951433 1109 8 0.600963496526731 1109 9 0.24079439103797284 1109 10-11 0.6396521008891582 1109 12-13 0.9236350345770958 1109 14-15 1.0741027078722922 1109 16-17 1.2104238232597666 1109 18-19 1.1181098028123984 1109 20-21 1.0941401414628231 1109 22-23 1.5119333558647199 1109 24-25 1.7900028822898335 1109 26-27 1.639883696452749 1109 28-29 1.5914263662940655 1109 30-31 2.0637398484736096 1109 32-33 2.898190786344138 1109 34-35 2.826318306543847 1109 36-37 3.2697564676178885 1109 38-39 2.431899243042622 1109 40-41 2.7119329060475934 1109 42-43 2.008752198260261 1109 44-45 1.691448736966052 1109 46-47 2.74733193725789 1109 48-49 2.350137360205487 1109 50-51 2.6311966786499354 1109 52-53 2.9241653221064254 1109 54-55 3.1848316407947834 1109 56-57 3.600357207509891 1109 58-59 4.241918828549675 1109 60-61 4.882038185313785 1109 62-63 5.562616267735592 1109 64-65 5.641459745421017 1109 66-67 6.426252022810729 1109 68-69 6.6278797089764545 1109 70-71 6.993570889717965 1109 72-73 4.274158413721125 1109 74-75 3.6858386739366473 1109 76-77 4.063567941327506 1109 78-79 3.7339573626315747 1109 80-81 2.1737643522878933 1109 82-83 3.7963210905142155 1109 84-85 4.307796297910244 1109 86-87 3.289625321627934 1109 88-89 2.8795375692100293 1109 90-91 3.4299337269352392 1109 92-93 2.9954651079392036 1109 94-95 2.1276209996870357 1109 96-97 2.4157709541624364 1109 98-99 1.2146137764566376 1109 100-101 3.022217937086989 1110 1 0.06019445548913893 1110 2 -0.8475950984735974 1110 3 -0.15805506055742313 1110 4 0.10921363687340602 1110 5 0.1355317668596001 1110 6 0.1657714724007633 1110 7 -0.17399343165779868 1110 8 0.08625761417378897 1110 9 0.3694708616262048 1110 10-11 -0.42284789911084175 1110 12-13 -1.4348208477758462 1110 14-15 -0.5674414097747658 1110 16-17 -0.8392085296814074 1110 18-19 -1.1465960795405437 1110 20-21 -0.8893157408901189 1110 22-23 -0.14799311472351206 1110 24-25 -0.18426182359251442 1110 26-27 -1.082542774135483 1110 28-29 -3.0372501042941664 1110 30-31 -3.5355248574087383 1110 32-33 -4.85732391953821 1110 34-35 -5.101990516985559 1110 36-37 -3.6732582382644594 1110 38-39 -4.14530663931032 1110 40-41 -4.084022976305349 1110 42-43 -3.494924272327971 1110 44-45 -4.1155365571516 1110 46-47 -5.059653356859762 1110 48-49 -4.567142051559223 1110 50-51 -3.4441709684088906 1110 52-53 -3.5960552661288716 1110 54-55 -3.3666389474405065 1110 56-57 -2.925378086607754 1110 58-59 -3.04484587733268 1110 60-61 -4.018697108803867 1110 62-63 -3.422677849911466 1110 64-65 -5.310746136931925 1110 66-67 -5.336615624248093 1110 68-69 -3.9566791145529585 1110 70-71 -2.94944381616439 1110 72-73 -5.494223939220053 1110 74-75 -5.580705443710411 1110 76-77 -6.024667352790139 1110 78-79 -5.243983813839012 1110 80-81 -5.294985647712107 1110 82-83 -3.1908112624269585 1110 84-85 -2.6094831138544627 1110 86-87 -4.063315854842653 1110 88-89 -3.1590653719664417 1110 90-91 -3.5810956848294637 1110 92-93 -3.070711362649032 1110 94-95 -1.3025260591364933 1110 96-97 -3.2882731634846216 1110 98-99 -3.6052391647198334 1110 100-101 -3.1450614746777177 1111 1 -2.344567449272766 1111 2 -0.7880712889497872 1111 3 -0.30984077484313843 1111 4 -1.3312625536027838 1111 5 -0.5281587093308744 1111 6 -1.0663713847420908 1111 7 -0.3347077173720834 1111 8 -0.40183762392145184 1111 9 -0.1989815193261748 1111 10-11 -0.19368123244417745 1111 12-13 -1.0077375144425176 1111 14-15 -0.923096171679525 1111 16-17 -1.0430775773004584 1111 18-19 -0.47992941287387225 1111 20-21 -1.3610419313663087 1111 22-23 -0.7625764480568407 1111 24-25 -0.7884284902591858 1111 26-27 -2.1346261074688186 1111 28-29 -0.5878453423894072 1111 30-31 -2.7527867621706434 1111 32-33 -1.2992882052524948 1111 34-35 -0.27312146936650805 1111 36-37 -1.1420082382644594 1111 38-39 -1.4280447345484149 1111 40-41 -2.981344404876779 1111 42-43 -2.8133766532803506 1111 44-45 -2.2301198904849358 1111 46-47 -2.8885224044788096 1111 48-49 -2.8543444325116027 1111 50-51 -2.139111444599365 1111 52-53 -2.633257647081251 1111 54-55 -2.277353233154791 1111 56-57 -1.3926399913696592 1111 58-59 -2.003179210666012 1111 60-61 -1.8966732992800566 1111 62-63 -0.7441064213400352 1111 64-65 -0.5994366131223998 1111 66-67 0.15594389956143218 1111 68-69 -0.16203625741010086 1111 70-71 1.177044279073705 1111 72-73 -1.074581082077195 1111 74-75 -1.692312586567553 1111 76-77 -2.6437149718377597 1111 78-79 -0.8332695281247275 1111 80-81 -0.3738546953311541 1111 82-83 -0.9809898338555278 1111 84-85 -1.5871616852830321 1111 86-87 -2.7299825215093207 1111 88-89 0.2590893899383211 1111 90-91 -0.3697861610199382 1111 92-93 -0.4457113626490319 1111 94-95 -0.7281212972317341 1111 96-97 2.3471435031820427 1111 98-99 1.046546549565882 1111 100-101 -0.7060733794396228 1112 1 0.8101944554891389 1112 2 0.35240490152640547 1112 3 0.5544449394425754 1112 4 0.20921363687340744 1112 5 0.29803176685960153 1112 6 0.2907714724007633 1112 7 0.4510065683422013 1112 8 -0.06374238582620961 1112 9 0.48197086162620195 1112 10-11 0.6646521008891568 1112 12-13 0.38392915222414814 1112 14-15 0.08880859022523424 1112 16-17 -0.9704585296814017 1112 18-19 -0.7465960795405451 1112 20-21 -0.2955657408901189 1112 22-23 0.2457568852764851 1112 24-25 0.8032381764074827 1112 26-27 0.6737072258645185 1112 28-29 -0.5247501042941636 1112 30-31 0.3769751425912631 1112 32-33 1.2614260804617885 1112 34-35 1.729259483014438 1112 36-37 2.3579917617355406 1112 38-39 1.2171933606896772 1112 40-41 1.6472270236946542 1112 42-43 1.5925757276720276 1112 44-45 1.6782134428484028 1112 46-47 1.9340966431402435 1112 48-49 3.251607948440771 1112 50-51 3.7620790315911066 1112 52-53 3.2476947338711284 1112 54-55 3.4583610525594963 1112 56-57 2.6121219133922473 1112 58-59 1.405154122667323 1112 60-61 1.8688028911961325 1112 62-63 1.6023221500885327 1112 64-65 1.726753863068076 1112 66-67 2.482134375751908 1112 68-69 3.0558208854470443 1112 70-71 1.0068061838356108 1112 72-73 -0.4067239392200541 1112 74-75 0.10054455628958792 1112 76-77 -1.2246673527901386 1112 78-79 -0.793983813839013 1112 80-81 -0.6262356477121038 1112 82-83 -1.5095612624269599 1112 84-85 -0.840733113854462 1112 86-87 0.011684145157346393 1112 88-89 0.5221846280335569 1112 90-91 0.5564043151705356 1112 92-93 -0.020711362649031173 1112 94-95 -0.915026059136494 1112 96-97 -0.8695231634846188 1112 98-99 -0.7927391647198334 1112 100-101 -0.8763114746777205 1113 1 -0.018752912931915944 1113 2 -0.17654246689465225 1113 3 -0.911344534241632 1113 4 -0.24604952102133382 1113 5 0.3164528194911824 1113 6 -0.4197548433887093 1113 7 -0.31215132639464116 1113 8 -0.0584792279314712 1113 9 -0.4496080857422129 1113 10-11 -0.7550847412161019 1113 12-13 -0.7621234793547913 1113 14-15 -0.08224404135371088 1113 16-17 -0.5217743191550923 1113 18-19 -0.8834381848037012 1113 20-21 -0.565302582995379 1113 22-23 0.1497042536975428 1113 24-25 -0.5428144551714595 1113 26-27 -0.8802401425565378 1113 28-29 -0.24448694639942659 1113 30-31 0.7309225110123165 1113 32-33 1.7758997646723103 1113 34-35 2.284522640909181 1113 36-37 0.5158864985776503 1113 38-39 1.693509150163365 1113 40-41 0.4472270236946514 1113 42-43 1.6728388855667689 1113 44-45 1.8926871270589274 1113 46-47 3.001201906298128 1113 48-49 1.473976369493407 1113 50-51 0.6265527158016297 1113 52-53 0.7476947338711284 1113 54-55 0.5083610525594935 1113 56-57 -0.2510359813445966 1113 58-59 0.7775225437199538 1113 60-61 2.2674871017224483 1113 62-63 3.7483747816674793 1113 64-65 4.771490705173335 1113 66-67 3.3163449020676943 1113 68-69 2.191347201236514 1113 70-71 -0.8918780266907049 1113 72-73 -0.1159344655358403 1113 74-75 -0.49682386476304075 1113 76-77 -0.1299305106848756 1113 78-79 1.3020688177399329 1113 80-81 0.33165908912999953 1113 82-83 1.9851755796783053 1113 84-85 2.352687938777116 1113 86-87 2.1208946714731347 1113 88-89 1.2603425227704008 1113 90-91 -0.3935956848294637 1113 92-93 -0.6825534679121894 1113 94-95 0.00997394086350667 1113 96-97 -0.4247863213793579 1113 98-99 0.14805030896437898 1113 100-101 0.7513201042696522 1114 1 0.5601944554891389 1114 2 0.0024049015264040463 1114 3 -0.045555060557425975 1114 4 0.20921363687340744 1114 5 -0.8019682331403999 1114 6 -0.15922852759923956 1114 7 -0.04899343165779868 1114 8 0.08625761417378897 1114 9 0.5819708616262034 1114 10-11 -0.26034789911084033 1114 12-13 -0.4410708477758476 1114 14-15 0.16380859022523708 1114 16-17 0.3045414703185898 1114 18-19 0.20340392045945066 1114 20-21 0.3544342591098797 1114 22-23 0.5207568852764908 1114 24-25 0.5032381764074856 1114 26-27 0.9487072258645171 1114 28-29 0.375249895705835 1114 30-31 0.8769751425912631 1114 32-33 0.5364260804617871 1114 34-35 0.6292594830144438 1114 36-37 0.2579917617355463 1114 38-39 2.5171933606896815 1114 40-41 1.1222270236946486 1114 42-43 2.642575727672032 1114 44-45 2.028213442848397 1114 46-47 2.8090966431402435 1114 48-49 1.1766079484407754 1114 50-51 1.4620790315911094 1114 52-53 1.022694733871127 1114 54-55 0.9333610525594906 1114 56-57 1.1121219133922402 1114 58-59 2.355154122667326 1114 60-61 -0.006197108803867479 1114 62-63 -0.34767784991146655 1114 64-65 -1.9982461369319253 1114 66-67 -1.3428656242480947 1114 68-69 1.6808208854470443 1114 70-71 0.13180618383561082 1114 72-73 -0.4067239392200541 1114 74-75 -0.6744554437104107 1114 76-77 -0.2746673527901393 1114 78-79 -0.5189838138390108 1114 80-81 -0.6012356477121052 1114 82-83 -2.434561262426957 1114 84-85 -0.9157331138544613 1114 86-87 -1.063315854842653 1114 88-89 -1.1278153719664417 1114 90-91 0.2064043151705377 1114 92-93 -0.020711362649031173 1114 94-95 0.6599739408635052 1114 96-97 0.48047683651537554 1114 98-99 -0.09273916471983412 1114 100-101 -1.151311474677719 1115 1 0.10564900094368568 1115 2 -0.46123146210996424 1115 3 -1.1410096060119699 1115 4 0.08648636414613264 1115 5 0.08439540322323325 1115 6 -0.3910467094174166 1115 7 -1.1853570680214318 1115 8 0.10898488690106234 1115 9 0.04560722526257166 1115 10-11 -0.5194388082017483 1115 12-13 0.11347460676960708 1115 14-15 0.2706267720434141 1115 16-17 -0.06364034786322037 1115 18-19 0.26249482955036285 1115 20-21 0.12488880456442786 1115 22-23 -0.4746976601780588 1115 24-25 -0.34903455086524104 1115 26-27 0.13052540768269694 1115 28-29 0.8547953502512868 1115 30-31 -0.1434794028632851 1115 32-33 -0.37721028317456984 1115 34-35 -1.9798314260764656 1115 36-37 -2.801099147355366 1115 38-39 -3.5146248211285 1115 40-41 -2.0982275217598954 1115 42-43 -0.3187879086916112 1115 44-45 -0.03315019351524029 1115 46-47 -1.704539720496122 1115 48-49 -2.1779375061046764 1115 50-51 -3.4811027865907107 1115 52-53 -2.1159416297652385 1115 54-55 -2.42345712925869 1115 56-57 -1.5560599047895742 1115 58-59 -1.6471186046054065 1115 60-61 -1.3880152906220466 1115 62-63 -0.8772233044569191 1115 64-65 -0.009609773295562007 1115 66-67 -0.2996838060662732 1115 68-69 -0.5191791145529585 1115 70-71 -4.622739270709843 1115 72-73 -7.386269393765506 1115 74-75 -6.390364534619502 1115 76-77 -6.683758261881049 1115 78-79 -6.618983813839012 1115 80-81 -7.871690193166652 1115 82-83 -7.705015807881503 1115 84-85 -6.583914932036281 1115 86-87 -7.267861309388108 1115 88-89 -6.07099719014826 1115 90-91 -6.302686593920374 1115 92-93 -4.627529544467212 1115 94-95 -5.671844240954675 1115 96-97 -6.88770498166644 1115 98-99 -5.667739164719833 1115 100-101 -5.801311474677718 1116 1 0.36971826501294913 1116 2 0.3071668062883077 1116 3 -0.30984077484313843 1116 4 0.0020707797305519193 1116 5 0.1385079573357899 1116 6 -0.06637138474209081 1116 7 0.14148275881839112 1116 8 -0.8304090524928753 1116 9 0.0867327663881099 1116 10-11 -0.24130028006322135 1116 12-13 0.06369105698605892 1116 14-15 0.43404668546332914 1116 16-17 -0.49545852968140736 1116 18-19 -0.6942151271595947 1116 20-21 -0.8610419313663087 1116 22-23 -0.07210025758065086 1116 24-25 -0.12176182359251442 1116 26-27 -0.27748325032595744 1116 28-29 -1.1116548661989256 1116 30-31 -1.4194534288373077 1116 32-33 -1.2516691576334509 1116 34-35 0.25068805444301034 1116 36-37 0.07227747602124879 1116 38-39 1.262431455927775 1116 40-41 -0.005153928686297604 1116 42-43 0.8294804895767882 1116 44-45 1.3413086809436408 1116 46-47 -0.19804621400262334 1116 48-49 0.8123222341550687 1116 50-51 0.09898379349586861 1116 52-53 0.8667423529187488 1116 54-55 1.2464562906547343 1116 56-57 0.7025981038684357 1116 58-59 -0.6698458773326799 1116 60-61 -3.1823875849943413 1116 62-63 -5.077439754673371 1116 64-65 -4.075627089312878 1116 66-67 -3.4869132432957137 1116 68-69 -2.2572743526481958 1116 70-71 -0.2515271494977256 1116 72-73 -1.550771558267673 1116 74-75 -3.216122110377075 1116 76-77 -1.858000686123475 1116 78-79 -3.1904123852675816 1116 80-81 -0.8262356477121067 1116 82-83 0.16186730900161095 1116 84-85 0.10331450519315766 1116 86-87 -0.7537920453188427 1116 88-89 -2.145672514823584 1116 90-91 -2.512643303877084 1116 92-93 0.8400029230652528 1116 94-95 1.4623548932444592 1116 96-97 -0.5576184015798589 1116 98-99 -2.143929640910308 1116 100-101 -0.5870257603920024 1201 1 0.39352778882247463 1201 2 0.9024049015264026 1201 3 0.48777827277591257 1201 4 0.359213636873406 1201 5 0.7813651001929358 1201 6 0.20743813906742758 1201 7 0.4510065683422013 1201 8 0.5029242808404533 1201 9 0.6819708616262048 1201 10-11 0.9313187675558297 1201 12-13 0.24226248555748953 1201 14-15 0.21380859022523424 1201 16-17 1.171208136985264 1201 18-19 1.4784039204594563 1201 20-21 1.3294342591098811 1201 22-23 1.1540902186098165 1201 24-25 1.044904843074157 1201 26-27 0.6570405591978457 1201 28-29 -0.5164167709608378 1201 30-31 -0.13969152407540975 1201 32-33 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52-53 0.9381709243473182 2312 54-55 1.2940753382737782 2312 56-57 0.5359314372017714 2312 58-59 0.6872969798101778 2312 60-61 1.579517176910418 2312 62-63 0.9701792929456801 2312 64-65 1.4719919583061696 2312 66-67 2.965467709085239 2312 68-69 2.0760589806851364 2312 70-71 2.367520469549895 2312 72-73 3.044466536970422 2312 74-75 1.4743540800991148 2312 76-77 1.189618361495576 2312 78-79 2.785778090922893 2312 80-81 0.6499548284783678 2312 82-83 1.3761530232873262 2312 84-85 2.912838314716968 2312 86-87 2.2462079546811573 2312 88-89 2.044803675652606 2312 90-91 0.892118600884821 2312 92-93 1.649526732589063 2312 94-95 0.43854536943493727 2312 96-97 0.4661911222296631 2312 98-99 0.6655941686135023 2312 100-101 0.34154566817942467 2313 1 -1.2017103064156238 2313 2 -0.3118808127593127 2313 3 -0.690793155795518 2313 4 -0.4265006488408787 2313 5 -0.1472063283784948 2313 6 0.2193429009721939 2313 7 0.23672085405648602 2313 8 0.26482904274521957 2313 9 -0.3894577098023646 2313 10-11 -0.40796694672989275 2313 12-13 -0.10297560968060537 2313 14-15 -0.20881045739381676 2313 16-17 -0.37641091063378695 2313 18-19 -0.5513579843024488 2313 20-21 -0.7658038361282138 2313 22-23 -0.42924311472351206 2313 24-25 -0.12176182359251442 2313 26-27 0.03204055919785276 2313 28-29 -0.7545120090560715 2313 30-31 0.36626085687697696 2313 32-33 -1.3230977290620203 2313 34-35 -3.0826452788903183 2313 36-37 -2.3562939525501676 2313 38-39 -1.9280447345484149 2313 40-41 -2.267058690591064 2313 42-43 -0.5514718913755914 2313 44-45 -1.1825008428658847 2313 46-47 -0.6504271663835723 2313 48-49 -0.9257730039401721 2313 50-51 -1.1629209684088906 2313 52-53 -2.514210028033631 2313 54-55 -1.944019899821459 2313 56-57 -0.8688304675601337 2313 58-59 -0.5031792106660156 2313 60-61 -0.6585780611848158 2313 62-63 0.6844650072313918 2313 64-65 -0.07562708931287432 2313 66-67 -0.15357990996237803 2313 68-69 0.957011361637516 2313 70-71 0.7246633266927525 2313 72-73 1.258752251256137 2313 74-75 1.4981636039086368 2313 76-77 -0.21514354326632912 2313 78-79 0.9762542813990827 2313 80-81 2.2928119713355137 2313 82-83 1.4237720709063737 2313 84-85 3.1271240290026796 2313 86-87 1.9128746213478216 2313 88-89 1.354327485176416 2313 90-91 0.6064043151705363 2313 92-93 2.2209553040176324 2313 94-95 0.5099739408635067 2313 96-97 1.4900006460391886 2313 98-99 2.5227370257563564 2313 100-101 1.55583138246514 2314 1 -1.7175833222886396 2314 2 -0.8198173206958188 2314 3 -1.4844439494463124 2314 4 -0.3074530297932583 2314 5 -1.0519682331403999 2314 6 -0.26478408315479385 2314 7 0.006562123897758454 2314 8 -0.4415201636039896 2314 9 0.4041930838484262 2314 10-11 0.16742987866693682 2314 12-13 -0.28551529222028904 2314 14-15 0.3943641457807914 2314 16-17 -0.49545852968140736 2314 18-19 -0.7299294128738794 2314 20-21 -1.226121296445676 2314 22-23 -1.623687559167955 2314 24-25 -1.7884284902591858 2314 26-27 -1.4679594408021543 2314 28-29 -0.7941945487386093 2314 30-31 -1.7091359685198526 2314 32-33 -3.76357391953821 2314 34-35 -3.0985182947633376 2314 36-37 -2.225341571597788 2314 38-39 -3.138362194865877 2314 40-41 -0.5805507540831272 2314 42-43 -0.6268687167724138 2314 44-45 -0.17456433492937862 2314 46-47 -0.8964589124153193 2314 48-49 -1.667836496003666 2314 50-51 -0.9684765239644477 2314 52-53 -1.5578608216844287 2314 54-55 0.3139166081150506 2314 56-57 -0.01565586438553268 2314 58-59 -0.6976236551104584 2314 60-61 -1.372863775470531 2314 62-63 -2.03378896102258 2314 64-65 -2.5954683591541468 2314 66-67 -1.8123100686925362 2314 68-69 -2.102512447886294 2314 70-71 -3.41819381616439 2314 72-73 -2.2095017169978313 2314 74-75 -3.021677665932632 2314 76-77 -2.6357784639012536 2314 78-79 -2.8689838138390122 2314 80-81 -1.8540134254898852 2314 82-83 -0.5206723735380692 2314 84-85 -1.8768442249655735 2314 86-87 -1.7022047437315422 2314 88-89 0.35829573914466906 2314 90-91 -0.9213734626072423 2314 92-93 -2.834600251537921 2314 94-95 0.34330727419684237 2314 96-97 -0.43063427459573234 2314 98-99 -0.695516942497612 2314 100-101 -1.2457559191221605 2315 1 -0.0761691808744942 2315 2 -0.824867825746324 2315 3 -1.186464151466513 2315 4 -0.2771499994902271 2315 5 0.08439540322323325 2315 6 -0.16377398214468997 2315 7 -1.1853570680214318 2315 8 -0.7546514767353045 2315 9 1.5267980802491365E-4 2315 10-11 -0.01943880820174826 2315 12-13 -2.204707211412206 2315 14-15 -2.7066459552293125 2315 16-17 -1.9954585296814074 2315 18-19 -2.032959715904184 2315 20-21 -0.8523839227083059 2315 22-23 -0.7019703874507854 2315 24-25 -2.417216369047061 2315 26-27 -1.7103836832263895 2315 28-29 -2.008841013385073 2315 30-31 -1.4843884937723786 2315 32-33 -0.46811937408366333 2315 34-35 0.3156231193780812 2315 36-37 -0.07382642008263929 2315 38-39 0.4626479061442268 2315 40-41 0.5835906600582916 2315 42-43 -1.2733333632370645 2315 44-45 -0.21496837533342017 2315 46-47 0.5681875522311444 2315 48-49 1.2084261302589638 2315 50-51 0.8370790315911094 2315 52-53 0.6340583702347686 2315 54-55 1.4629065071049467 2315 56-57 2.1030310043013394 2315 58-59 2.2392450317582266 2315 60-61 3.1119847093779534 2315 62-63 3.8955039682703543 2315 64-65 4.467662953977165 2315 66-67 3.90486164847918 2315 68-69 1.9126390672652214 2315 70-71 0.05907891110833674 2315 72-73 0.0682760607799473 2315 74-75 1.2914536471986828 2315 76-77 -0.411030989153776 2315 78-79 0.5173798225246209 2315 80-81 1.8328552613788034 2315 82-83 0.7949841921184948 2315 84-85 2.075175977054627 2315 86-87 1.4594114178846205 2315 88-89 1.792639173488105 2315 90-91 2.288222496988716 2315 92-93 2.554288637350968 2315 94-95 1.7599739408635067 2315 96-97 0.6122950183335583 2315 98-99 0.1958971989165299 2315 100-101 0.9714157980495557 2316 1 0.040194455489135805 2316 2 -0.15759509847359965 2316 3 -0.1555550605574254 2316 4 -0.380786363126596 2316 5 -0.17196823314039733 2316 6 -0.5692285275992361 2316 7 0.031006568342199614 2316 8 0.1962576141737884 2316 9 -0.6580291383737986 2316 10-11 -0.34034789911084573 2316 12-13 0.27892915222415837 2316 14-15 -0.4211914097747709 2316 16-17 -1.0154585296814034 2316 18-19 -0.746596079540538 2316 20-21 0.3294342591098811 2316 22-23 -0.16924311472351405 2316 24-25 -0.1017618235925184 2316 26-27 -0.46129277413547953 2316 28-29 -1.1097501042941715 2316 30-31 -0.9280248574087366 2316 32-33 -0.9935739195382141 2316 34-35 -0.8407405169855551 2316 36-37 -2.1820082382644586 2316 38-39 -0.9328066393103214 2316 40-41 -2.2527729763053443 2316 42-43 -2.3524242723279727 2316 44-45 -1.4567865571516023 2316 46-47 -4.460903356859763 2316 48-49 -3.773392051559224 2316 50-51 -4.482920968408891 2316 52-53 -3.4723052661288705 2316 54-55 -3.5516389474405052 2316 56-57 -4.387878086607753 2316 58-59 -3.749845877332678 2316 60-61 -3.246197108803866 2316 62-63 -5.272677849911467 2316 64-65 -4.503246136931924 2316 66-67 -3.747865624248096 2316 68-69 -2.4991791145529554 2316 70-71 -3.57819381616439 2316 72-73 -2.991723939220055 2316 74-75 -3.789455443710409 2316 76-77 -2.1446673527901368 2316 78-79 -2.9789838138390117 2316 80-81 -3.446235647712104 2316 82-83 -2.2295612624269587 2316 84-85 -2.9357331138544644 2316 86-87 -1.603315854842652 2316 88-89 -2.802815371966439 2316 90-91 -2.453595684829466 2316 92-93 -1.485711362649031 2316 94-95 -1.9900260591364933 2316 96-97 0.7404768365153807 2316 98-99 -0.4077391647198354 2316 100-101 -1.381311474677716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 8.0 15 44.0 16 95.0 17 241.0 18 397.0 19 448.0 20 485.0 21 564.0 22 449.0 23 347.0 24 387.0 25 695.0 26 1315.0 27 1461.0 28 1698.0 29 1548.0 30 576.0 31 255.0 32 199.0 33 310.0 34 391.0 35 643.0 36 1097.0 37 2184.0 38 1509.0 39 82.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.16152564765351 22.66643690045379 12.619909242345914 32.55212820954679 2 11.70902415237221 18.025357122368195 45.832138144684755 24.433480580574837 3 13.82594228673054 31.811141070506572 41.804830474442085 12.55808616832081 4 6.9416556709311 31.552980322414093 21.00281108370145 40.50255292295336 5 6.999024726062762 35.27049509494579 41.01313751362515 16.717342665366303 6 18.553152429579484 37.74310137112042 17.136136767827434 26.567609431472665 7 11.657392002753715 34.18048304744421 16.14365211404968 38.01847283575239 8 21.50765876886008 34.68533073260283 27.43388216396076 16.37312833457633 9 16.189547358155014 12.11634444380701 13.303883885032414 58.39022431300557 10-11 18.989157248580117 25.07601399804945 27.852676266421895 28.08215248694854 12-13 22.895989903046296 21.6396075956629 25.701336698984566 29.763065802306237 14-15 20.136538351213357 23.16562446216511 17.945040445183867 38.75279674143767 16-17 23.243072686592853 34.69680454362917 17.560667775801733 24.49945499397625 18-19 24.99282886810854 25.8820492226493 20.15661752050944 28.96850438873272 20-21 22.302220182433597 24.858011588549136 19.39074063450175 33.44902759451552 22-23 18.673627445355976 38.1360793987723 19.52842636681774 23.661866789053985 24-25 24.0835293442717 39.6965176983535 19.03218403992886 17.18776891744593 26-27 25.67552062417532 41.414720899546786 19.654638288107396 13.255120188170503 28-29 21.631002237393147 31.426768401124434 23.742183466238313 23.200045895244106 30-31 27.299064884401353 30.058516436234296 24.37037461993001 18.272044059434343 32-33 21.347025414491423 32.50817509035626 29.45040445183868 16.694395043313637 34-35 20.294303252825426 22.534564855716827 31.389478515288854 25.781653376168894 36-37 16.100625322700935 35.884343984854574 25.575124777694914 22.439905914749584 38-39 18.323676209052834 20.22259193391085 33.664161551259255 27.78957030577706 40-41 15.730594917101715 18.805576272158795 38.506109804371526 26.957719006367963 42-43 23.191440536974355 16.27846939360909 38.70116459181917 21.828925477597384 44-45 13.550570822098559 23.99173885606104 38.83885032413516 23.618839997705237 46-47 23.922895989903044 15.033560897252022 29.524984223509836 31.51855888933509 48-49 21.2322873042281 14.233262578165338 32.2643566060467 32.270093511559864 50-51 16.714474212609716 16.65136825196489 38.62371636739143 28.01044116803396 52-53 14.617635247547472 20.558200906431072 45.36171189260513 19.462451953416327 54-55 13.679651196144798 22.873042280993634 34.95496529172165 28.49234123113992 56-57 21.702713556307728 29.866330101543227 23.495496529172165 24.93545981297688 58-59 11.063622282141013 37.284148930067126 35.40818082726177 16.24404796053009 60-61 19.04939475646836 46.69554242441627 17.26234868911709 16.99271412999828 62-63 11.011990132522516 55.84877517067294 15.851069932878206 17.28816476392634 64-65 10.99477941598302 54.417417245137976 15.833859216338709 18.753944122540304 66-67 11.571338420056222 52.57300212265504 15.673225861970053 20.182433595318685 68-69 13.163329699959842 49.32591360220297 16.02317709827319 21.487579599563993 70-71 14.921691239745282 46.97091388904825 15.971544948654696 22.135849922551778 72-73 16.135046755779932 41.74459296655384 17.239401067064424 24.8809592106018 74-75 16.03178245654294 40.50828982846652 17.425850496242326 26.03407721874821 76-77 17.606563019907064 39.530147438471694 17.54058860650565 25.3227009351156 78-79 18.220411909815844 38.70690149733234 17.793012449084962 25.27967414376685 80-81 17.488956456887156 38.133210946015716 17.600826114393897 26.777006482703232 82-83 17.560667775801733 38.22500143422638 18.573231598875566 25.641099191096323 84-85 18.690838161895474 36.29166427628937 19.141185244679022 25.876312317136136 86-87 19.829613906258963 35.31639033905112 18.74247031151397 26.111525443175953 88-89 19.617348402271816 34.65377775228042 19.410819803797832 26.318054041649937 90-91 19.53990017784407 33.69858298433825 20.440594343411163 26.320922494406517 92-93 19.57719006367965 32.60857093683667 20.98273191440537 26.83150708507831 94-95 20.27135563077276 31.051001090012047 21.711318914577475 26.966324364637718 96-97 19.603006138488897 30.864551660834145 22.51161723366416 27.020824967012793 98-99 19.44524123687683 31.248924330216283 23.01933337157937 26.28650106132752 100-101 19.258791807698927 31.484137456256096 22.83288394240147 26.424186793643507 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 111.0 1 66.0 2 18.5 3 20.0 4 17.0 5 9.0 6 14.0 7 20.0 8 30.0 9 36.5 10 25.0 11 18.5 12 14.0 13 15.0 14 19.5 15 20.5 16 21.5 17 21.5 18 31.0 19 39.5 20 39.5 21 38.5 22 54.5 23 63.0 24 72.5 25 96.0 26 113.5 27 144.5 28 161.5 29 173.0 30 209.0 31 232.5 32 239.0 33 267.0 34 300.0 35 350.5 36 422.5 37 508.0 38 590.0 39 633.0 40 689.5 41 758.0 42 760.5 43 713.5 44 664.5 45 594.0 46 536.5 47 548.0 48 575.0 49 638.5 50 857.5 51 947.0 52 666.0 53 379.0 54 311.5 55 320.5 56 312.0 57 288.0 58 281.5 59 252.0 60 203.0 61 162.5 62 131.5 63 115.0 64 92.5 65 72.0 66 62.0 67 47.5 68 33.5 69 31.0 70 24.0 71 20.5 72 16.0 73 14.5 74 18.5 75 15.5 76 11.5 77 10.5 78 13.0 79 10.0 80 4.0 81 2.0 82 1.5 83 2.5 84 2.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12621192128965636 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 17431.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.47604841948253 #Duplication Level Percentage of deduplicated Percentage of total 1 84.88654781199352 36.05645115024956 2 8.860075634792004 7.526820033274053 3 2.82279848730416 3.597039756755206 4 1.1345218800648298 1.9276002524238427 5 0.6212857914640735 1.3194882680282256 6 0.25661804430037816 0.6540072285009466 7 0.25661804430037816 0.7630084332511043 8 0.24311183144246357 0.8261143938959326 9 0.09454349000540249 0.3614250473294705 >10 0.5807671528903295 4.905054213757099 >50 0.08103727714748785 2.4496586541219667 >100 0.10804970286331712 7.991509379840514 >500 0.01350621285791464 4.055992197808502 >1k 0.04051863857374392 27.565830990763583 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 2705 15.518329413114564 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 1056 6.058172221903505 RNA PCR Primer, Index 33 (95% over 24bp) ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 1044 5.989329355745511 RNA PCR Primer, Index 33 (95% over 22bp) TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 707 4.055992197808502 RNA PCR Primer, Index 33 (96% over 29bp) CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT 428 2.4553955596351327 RNA PCR Primer, Index 33 (96% over 27bp) CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG 238 1.3653835121335551 RNA PCR Primer, Index 15 (95% over 24bp) ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC 153 0.8777465435144284 RNA PCR Primer, Index 15 (96% over 25bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 130 0.7457977167116058 No Hit CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA 117 0.6712179450404452 RNA PCR Primer, Index 33 (96% over 29bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 116 0.665481039527279 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 108 0.6195857954219495 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 103 0.5909012678561184 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 0.5163214961849578 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC 84 0.4819000631059607 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 73 0.41879410246113247 No Hit TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT 62 0.35568814181630426 RNA PCR Primer, Index 33 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60 0.3442143307899719 No Hit TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC 58 0.3327405197636395 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39 0.2237393150134817 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT 39 0.2237393150134817 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 38 0.21800240950031552 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 37 0.21226550398714936 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT 37 0.21226550398714936 No Hit GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGG 34 0.19505478744765076 No Hit CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACAAGAGGC 32 0.18358097642131835 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 29 0.16637025988181975 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTCTTCT 29 0.16637025988181975 Illumina PCR Primer Index 4 (95% over 21bp) TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 29 0.16637025988181975 No Hit ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT 27 0.15489644885548737 RNA PCR Primer, Index 15 (96% over 27bp) GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.14915954334232115 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26 0.14915954334232115 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA 25 0.14342263782915496 No Hit GCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA 22 0.12621192128965636 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA 22 0.12621192128965636 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT 22 0.12621192128965636 No Hit GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC 21 0.12047501577649017 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATACAC 20 0.11473811026332396 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCT 20 0.11473811026332396 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTC 19 0.10900120475015776 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.017210716539498596 0.0 22-23 0.0 0.0 0.0 0.09179048821065916 0.0 24-25 0.0 0.0 0.0 0.23808157879639721 0.0 26-27 0.0 0.0 0.0 0.4446101772703803 0.0 28-29 0.0 0.0 0.0 0.9465894096724227 0.0 30-31 0.0 0.0 0.0 1.6149389019562848 0.0 32-33 0.0 0.0 0.0 2.3521312603981412 0.0 34-35 0.0 0.0 0.0 3.447880213412885 0.0 36-37 0.0 0.0 0.0 4.6727095404738686 0.0 38-39 0.0 0.0 0.0 5.920486489587516 0.0 40-41 0.0 0.0 0.0 7.9083242498996045 0.0 42-43 0.0 0.0 0.0 8.989730939131432 0.0 44-45 0.0 0.0 0.0 9.53473696288222 0.0 46-47 0.0 0.0 0.0 10.016637025988182 0.0 48-49 0.0 0.0 0.0 10.57024840800872 0.0 50-51 0.0 0.0 0.0 11.103780620733176 0.0 52-53 0.0 0.0 0.0 11.370546727095405 0.0 54-55 0.0 0.0 0.0 11.944237278412025 0.0 56-57 0.0 0.0 0.0 12.603981412426137 0.0 58-59 0.0 0.0 0.0 12.925248121163445 0.0 60-61 0.0 0.0 0.0 13.100223739315012 0.0 62-63 0.0 0.0 0.0 13.243646377144168 0.0 64-65 0.0 0.0 0.0 13.335436865354827 0.0 66-67 0.0 0.0 0.0 13.498938672480065 0.0 68-69 0.0 0.0 0.0 13.75423096781596 0.0 70-71 0.0 0.0 0.0 13.949285755263611 0.0 72-73 0.0 0.0 0.0 14.115656015145431 0.0 74-75 0.0 0.0 0.0 14.304973897079915 0.0 76-77 0.0 0.0 0.0 14.43118581836957 0.0 78-79 0.0 0.0 0.0 14.56887155068556 0.0 80-81 0.0 0.0 0.0 14.712294188514715 0.0 82-83 0.0 0.0 0.0 14.898743617692617 0.0 84-85 0.0 0.0 0.0 15.065113877574436 0.0 86-87 0.0 0.0 0.0 15.257300212265505 0.0 88-89 0.0 0.002868452756583099 0.0 15.524066318627732 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 25 5.1306553E-5 76.00001 1 ACCCATG 25 5.1306553E-5 76.00001 3 TACCCAT 25 5.1306553E-5 76.00001 2 CGCAGAA 50 4.0029408E-7 57.000004 9 TATACAC 560 0.0 44.95536 3 TTATACA 550 0.0 44.909092 2 CTTATAC 540 0.0 44.86111 1 ATACACA 630 0.0 39.20635 4 TACACAT 640 0.0 38.59375 5 GGTATCA 260 0.0 32.884617 1 ACACATC 790 0.0 31.265821 6 CACATCT 795 0.0 31.069181 7 ACATCTC 815 0.0 30.889568 8 CATCTCC 870 0.0 28.93678 9 ACACCCC 35 0.008282174 27.142859 78-79 CATGGGA 70 5.538968E-7 27.142859 16-17 GTATCAA 330 0.0 25.909092 2 AACGCAG 330 0.0 24.469698 7 TATCAAC 335 0.0 24.104479 3 ATCAACG 335 0.0 24.104479 4 >>END_MODULE