FastQCFastQC Report
Fri 10 Feb 2017
SRR2031778_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031778_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51475
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12322.39339485186984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8211.594949004371054No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7681.4919864011656145No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7311.4201068479844585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.9286061194754734No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.6779990286546868No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.5925206410879067No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.48372996600291407No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.3050024283632831No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1390.27003399708596404No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.2156386595434677No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.21369596891694995No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.20592520641087908No Hit
TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.12433220009713453No Hit
GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT620.12044681884409907No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT610.11850412821758136No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT600.11656143759106363No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG570.11073336571151043No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCT250.004994128457.09
GGTATCA5950.056.6806761
TACCCAT459.5394626E-442.222222
ACCCATG459.5394626E-442.222223
GTATCAA8400.040.148812
CGCAGAA1357.2759576E-1238.70379
GCAGAGA603.7234713E-935.62510-11
CAACGCA9900.034.065666
TATCAAC10050.033.5572173
TCAACGC10050.033.5572175
ATCAACG10100.033.391094
AACGCAG10050.033.084587
GTACCCA600.003916275731.6666681
AGCCTTT300.00393440131.66666836-37
ACGCAGA10650.030.7746498
TAGATTT650.00579301129.2307664
CGCAGAG9500.029.09
ATGGGGA350.0083567327.14285918-19
GAGTACT5250.026.69047412-13
GTACTTT5550.025.24774714-15
Produced by FastQC (version 0.11.5)